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REVIEW article

Front. Plant Sci.

Sec. Technical Advances in Plant Science

This article is part of the Research TopicAdvancements of Haploid Technology in Crops: New Horizons in Breeding and GeneticsView all 4 articles

Advances in Doubled Haploid Technology for Rice Breeding: Mechanisms, Applications, and Future Perspectives

Provisionally accepted
Yiling  LinYiling Lin1Fangming  MaoFangming Mao2Yuheng  HuangYuheng Huang1Derun  HunagDerun Hunag1Kejian  WangKejian Wang1Song  YanSong Yan2*Chaolei  LiuChaolei Liu3*
  • 1China National Rice Research Institute, Hangzhou, China
  • 2Jiangxi Academy of Agricultural Sciences, Nanchang, China
  • 3China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China

The final, formatted version of the article will be published soon.

Production of homozygous inbred lines is crucial in rice breeding programs. Doubled haploid (DH) technology enables the efficient development of pure lines and significantly shortens the breeding cycle compared to conventional methods. This review summarizes recent advances in the four key steps of DH technology mediated by haploid inducers in rice: (1) exploration of haploid induction genes and development of high-efficiency haploid inducers, (2) rapid identification of haploids using molecular and morphological markers, (3) chromosome doubling of haploid plants through colchicine treatment, and (4) application of DH technology in rice breeding. Furthermore, current challenges and issues associated with each step are discussed. With ongoing advancements and the resolution of existing limitations, DH technology based on haploid inducers is anticipated to become a fundamental and widely adopted tool in rice breeding.

Keywords: DH technology, Haploid inducer, haploid induction, rice, Breeding

Received: 19 Sep 2025; Accepted: 27 Nov 2025.

Copyright: © 2025 Lin, Mao, Huang, Hunag, Wang, Yan and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Song Yan
Chaolei Liu

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