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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Plant Biotechnology

This article is part of the Research TopicNew Biotechnological Advances for Optimizing Plant-Soil Relationships in Sustainable AgricultureView all articles

Humic field biostimulation as a sustainable agricultural practice to increase yield of main grains: Evidence from on-farm trials

Provisionally accepted
Juan  IzquierdoJuan Izquierdo1Osvin  ArriagadaOsvin Arriagada2Gustavo  García-PintosGustavo García-Pintos3Martín  García-PintosMartín García-Pintos3Marcelo  García-PintosMarcelo García-Pintos4Rodomiro  OrtizRodomiro Ortiz5*
  • 1Servicios para el Desarrollo Rural Integral y la Agricultura (SEDRA), Punta del Este, Uruguay
  • 2Centro de Estudios en Alimentos Procesados, Talca, Chile
  • 3BIOCIS, Paysandú, Uruguay
  • 4BIOCIS, Montevideo, Uruguay
  • 5Swedish University of Agricultural Sciences, Uppsala, Sweden

The final, formatted version of the article will be published soon.

Conventional agriculture relies heavily on synthetic fertilizers and pesticides, raising questions about its long-term sustainability. The use of biostimulants is an environmentally friendly approach to improve crop yields. However, most results have been obtained under controlled conditions, making it necessary to evaluate them under commercial production. Therefore, the objective of this study was to assess the effect of a single foliar spray of a humic biostimulant (HB), at a critical stage of the growth cycle, on the yield and profitability of soybeans (R3-R4), rice (V5-V9), maize (V6), wheat (Z1.6), and barley (Z1.6), as measured by yield responses and net economic returns. For this purpose, on-farm strip trials (OFT) (n=448) were installed on commercial farms from 2014 to 2024 in the main production zones of Uruguay. The HB increased the average yield in all five crops. The overall mean yield response was significant, ranging from 7.6% to 15.7% in rice and maize, respectively. In barley, HB showed greater effectiveness in lower-yielding sites, reaching ~4 t ha-1. In rice, a tendency towards a greater impact in low and high-yield OFTs was observed. While in maize, wheat, and soybeans, HB had a relatively constant effect. The net economic returns were 68 and 164 US$ ha-1 for wheat and rice, respectively. Similarly, the probability of exceeding the break-even cost ranged from 79.7 % for wheat to 87.5% for rice. To our knowledge, this is the first study to demonstrate that a single foliar application of an HB at a critical stage of development increases crop yield and profitability for farmers under OFT conditions over several years and multiple sites. Therefore, this practice can be applied by Uruguayan farmers to increase yields and economic profits sustainably.

Keywords: barley, Economic profitability, humic biostimulant, Maize, rice, Soybean, sustainable agriculture, wheat

Received: 21 Sep 2025; Accepted: 17 Nov 2025.

Copyright: © 2025 Izquierdo, Arriagada, García-Pintos, García-Pintos, García-Pintos and Ortiz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Rodomiro Ortiz, rodomiro.ortiz@slu.se

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