ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
This article is part of the Research TopicTrends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding - Volume IIView all 5 articles
A 4NsL chromosome segment from Leymus mollis confers stripe rust and Fusarium head blight resistance in wheat
Provisionally accepted
Wanquan Ji1*
Xin Du1,2Yanlong Jin3Wei Ren1
Dong Dong Ge1Xiaomin Xu1Xiaoqi Zhao1
Yanzhen Wang4Tingting Li5
Jixin Zhao1Changyou Wang1
Tingdong Li1Chunhuan Chen1Xinlun Liu1Pingchuan Deng1*- 1Northwest A&F University, Xianyang, China
- 2Anhui Academy of Agricultural Science, Hefei, China
- 3Shihezi University, Shihezi, China
- 4Shanxi Agricultural University, Taiyuan, China
- 5Yangling Vocational and Technical College, Xianyang, China
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As a tertiary genetic resource for wheat improvement, Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) harbors valuable traits including disease resistance and stress tolerance. Here, we investigated the characterisation of two novel wheat-L. mollis translocation lines (M892 and M956) developed from F4 progenies of a cross between common wheat Norin 26+3C (gametocidal chromosome) and a wheat-L. mollis 4Ns disomic addition line M852. Resistance evaluation revealed delineated that M892 exhibited susceptibility to stripe rust and Fusarium head blight (FHB, Type II), while M956 displayed high resistance to both pathogens. Both lines showed enhanced grain weight, with M892 additionally presenting dwarf. Integrated fluorescence in situ hybridization (GISH, sequential FISH-GISH) and wheat-Psathyrostachys huashanica 45K liquid array (GenoBaits®WheatplusPh) analyses delineated that M892 was a T5DL-4NsL-5DL·5DS insertional translocation line, whereas M956 was a T4NsL-5DL·5DS terminal translocation line. Genetic mapping localized the stripe rust resistance loci to a 98 Mb critical region (648-746 Mb) on chromosome 4NsL. Comparative transcriptomics identified 11,368 differentially expressed unigenes unique to M956, including 47 candidates functionally linked to disease resistance. Consequently, we developed eight 4NsL-specific molecular markers, two of which were associated with disease resistance. These markers enable detection of 4NsL chromatin in wheat breeding programs, providing a foundation for utilization of 4NsL genes resistant to stripe rust and FHB.
Keywords: Leymus mollis, Translocation line, pecific markers, stripe rust, Fusarium head blight
Received: 23 Sep 2025; Accepted: 30 Oct 2025.
Copyright: © 2025 Ji, Du, Jin, Ren, Ge, Xu, Zhao, Wang, Li, Zhao, Wang, Li, Chen, Liu and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Wanquan Ji, jiwanquan2008@126.com
Pingchuan Deng, dengpingchuan@nwafu.edu.cn
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