ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
This article is part of the Research TopicModelling Environmental and Crop Production Systems: Evaluating Impacts of Abiotic Stress on Crop Growth and Resource Use EfficiencyView all articles
Genome-wide identification of DNA-binding with one finger transcription factor genes in Chinese chestnut and their response to abiotic stress
Provisionally accepted
- 1Zhejiang Academy of Forestry, Hangzhou, China
- 2Chinese Academy of Forestry Research Institute of Subtropical Forestry, Fuyang, China
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DNA-binding with One Finger (Dof) proteins are unique single zinc finger transcription factors that play important roles in plant growth, development, and abiotic stress responses. However, the Dof genes in Chinese chestnut (Castanea mollissima) have not been studied yet. The transcription factor family in the Chinese chestnut genome was identified and analyzed using bioinformatics. The analysis results revealed that a total of 25 CmDof genes (CmDof1 – CmDof25) were identified in Chinese chestnut. Furthermore, we assessed the physicochemical properties, phylogeny, gene structures, cis-regulatory elements (CREs), and expression profiles.The 25 CmDof genes were categorized into five subfamilies according to the phylogeny analysis. Analysis of cis-acting elements revealed that the promoter region of the Dof gene in Chinese chestnut contains light regulation, plant growth and development, plant hormones, and stress-response elements to adapt to environmental changes. The RNA-seq data analysis indicated the potential roles of CmDof genes in the response to temperature stress, drought stress, and shade stress. Additionally, The expression levels of nine CmDof genes showed significant differences in their response to the three abiotic stresses,confirmed by RT-qPCR assays. Collectively, these data lay a foundation for further functional explorations of CmDof genes, especially concerning the possible application of multiple CmDof genes in breeding tolerant plants.
Keywords: DOF, gene family, IDENTIFICATION, expression analysis, stress response
Received: 23 Sep 2025; Accepted: 23 Oct 2025.
Copyright: © 2025 Xu, Jiang and Cheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shiming Cheng, 864569485@qq.com
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