Reactive Oxygen Species: Balancing Agents in Plants

Pravej Alam¹MOHAMMAD FAIZAN^2*Yamshi Arif³Maged M. Azzam⁴Shamsul Hayat³Shadma Afzal⁵Thamer Albalawi¹

• ¹Prince Sattam bin Abdulaziz University, Al Kharj, Saudi Arabia
• ²Maulana Azad National Urdu University, Hyderabad, India
• ³Aligarh Muslim University, Aligarh, India
• ⁴Other
• ⁵Uniwersytet Przyrodniczy w Poznaniu, Poznań, Poland

Reactive Oxygen Species (ROS) are highly reactive molecules derived from molecular oxygen, playing a dual role in plant systems as both signaling molecules and potential agents of cellular damage. This comprehensive review highlights the fundamental aspects of ROS biology in plants, beginning with the definition and chemical nature of ROS, followed by an in-depth discussion on their various types, including singlet oxygen (1O2), superoxide radicals (O2•–), hydrogen peroxide (H2O2), and hydroxyl radicals (OH•). The review outlines the primary sites of ROS production within plant cells, such as mitochondria, chloroplasts, and peroxisomes, and explains their integral roles throughout the plant life cycle, including growth, development, and senescence. Furthermore, the involvement of ROS in cell cycle regulation, cell division, and programmed cell death is discussed, emphasizing their critical role in maintaining cellular homeostasis. The review also sheds light on ROS-mediated signaling pathways and their interaction with key plant hormones, including strigolactones, salicylic acid, brassinosteroids, jasmonic acid, and karrikins, highlighting the complexity of ROS-hormone crosstalk in regulating stress responses and development. The damaging effects of uncontrolled ROS accumulation on DNA, lipids, proteins, and enzymes are thoroughly examined, underscoring their potential to disrupt cellular function. Methods of ROS detection in plant tissues are briefly presented, offering insights into current techniques used for quantifying and visualizing ROS. Overall, this review provides a detailed understanding of ROS dynamics in plant biology and serves as a valuable reference for future research aiming to manipulate ROS signaling for improved plant resilience and productivity.