The challenge of feeding the world's growing population necessitates increased agricultural productivity while maintaining food safety. Invasive plant pathogens, including viruses, fungi, nematodes, mycoplasma, and bacteria, pose significant crop threats, reducing productivity and causing economic losses. According to a recent FAO report, plant diseases cost the global economy approximately $220 billion. Traditionally, plant diseases are identified by visible symptoms, often when the disease is already advanced, making treatment difficult. Early identification and quantification of pathogens are crucial for ensuring food security and reducing crop loss.
Recent advancements in real-time diagnostic research have enabled high-sensitivity and high-specificity field-level disease monitoring. Current techniques, including enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), real-time PCR, multiplex PCR, fluorescence in situ hybridization (FISH), and flow cytometric detection (FCM), are often specific, time-consuming, and expensive. There is a growing need for rapid, accurate, and cost-effective diagnostics, particularly for emerging diseases or pathogens with subtle initial symptoms.
Innovative field-use diagnostic tools, such as portable systems interconnected with the Internet of Things (IoT), nanotechnology and biosensor-based diagnostics, isothermal nucleic acid visual detection systems (e.g., loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), and recombinase polymerase assay (RPA)), and CRISPR/Cas technologies, are critical in this context. These technologies enable the detection of pathogens and are suitable for operational contexts.
We propose this Research Topic consisting of research articles, reviews, and methods providing a comprehensive understanding of recent field-level diagnostics for real-time identification and timely management of plant diseases. The collection welcomes contributions from eminent plant pathologists and biologists with extensive crop and disease experience. This collection will be valuable to students, teachers, researchers, and crop stakeholders.
The success of our previous volume underscores the importance of this work, and we aim to continue this momentum with a second volume focused on the latest advancements in diagnostics.
We welcome submissions on, but not limited to, the below:
- Current trends in plant disease diagnosis and management
- Recent advancements in plant pathogen detection enabled by biosensors
- Recent advances in nucleic acid-based assays
- Bio, LFD and magnetic assays in plants
- Recent advances in plant field diagnostics
- Insights into isothermal amplification methods for real-time plant diagnosis
- Application of recombinase polymerase amplification techniques in plant disease diagnosis and management
- Role of DNA and RNA-based probes and next-generation sequencing in molecular diagnostics
- Novel tools in molecular diagnostics for plant pathogen detection
- Future of CRISPR/Cas technologies in plant pathogen detection
Keywords:
plant disease, plant-pathogen, detection, field-level diagnostics, fungi, bacteria, viruses, insects, nematodes, PCR, LAMP, RPA
Important Note:
All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.
The challenge of feeding the world's growing population necessitates increased agricultural productivity while maintaining food safety. Invasive plant pathogens, including viruses, fungi, nematodes, mycoplasma, and bacteria, pose significant crop threats, reducing productivity and causing economic losses. According to a recent FAO report, plant diseases cost the global economy approximately $220 billion. Traditionally, plant diseases are identified by visible symptoms, often when the disease is already advanced, making treatment difficult. Early identification and quantification of pathogens are crucial for ensuring food security and reducing crop loss.
Recent advancements in real-time diagnostic research have enabled high-sensitivity and high-specificity field-level disease monitoring. Current techniques, including enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), real-time PCR, multiplex PCR, fluorescence in situ hybridization (FISH), and flow cytometric detection (FCM), are often specific, time-consuming, and expensive. There is a growing need for rapid, accurate, and cost-effective diagnostics, particularly for emerging diseases or pathogens with subtle initial symptoms.
Innovative field-use diagnostic tools, such as portable systems interconnected with the Internet of Things (IoT), nanotechnology and biosensor-based diagnostics, isothermal nucleic acid visual detection systems (e.g., loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), and recombinase polymerase assay (RPA)), and CRISPR/Cas technologies, are critical in this context. These technologies enable the detection of pathogens and are suitable for operational contexts.
We propose this Research Topic consisting of research articles, reviews, and methods providing a comprehensive understanding of recent field-level diagnostics for real-time identification and timely management of plant diseases. The collection welcomes contributions from eminent plant pathologists and biologists with extensive crop and disease experience. This collection will be valuable to students, teachers, researchers, and crop stakeholders.
The success of our previous volume underscores the importance of this work, and we aim to continue this momentum with a second volume focused on the latest advancements in diagnostics.
We welcome submissions on, but not limited to, the below:
- Current trends in plant disease diagnosis and management
- Recent advancements in plant pathogen detection enabled by biosensors
- Recent advances in nucleic acid-based assays
- Bio, LFD and magnetic assays in plants
- Recent advances in plant field diagnostics
- Insights into isothermal amplification methods for real-time plant diagnosis
- Application of recombinase polymerase amplification techniques in plant disease diagnosis and management
- Role of DNA and RNA-based probes and next-generation sequencing in molecular diagnostics
- Novel tools in molecular diagnostics for plant pathogen detection
- Future of CRISPR/Cas technologies in plant pathogen detection
Keywords:
plant disease, plant-pathogen, detection, field-level diagnostics, fungi, bacteria, viruses, insects, nematodes, PCR, LAMP, RPA
Important Note:
All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.