Mini Review ARTICLE
GIP/MZT1 proteins orchestrate nuclear shaping
- Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Conventionné avec l’Université de Strasbourg, Strasbourg, France
The functional organization of the nuclear envelope (NE) is only just emerging in plants with the recent characterization of NE protein complexes and their molecular links to the actin cytoskeleton. The NE also plays a role in microtubule nucleation by recruiting γ-Tubulin Complexes (γ-TuCs) which contribute to the establishment of a robust mitotic spindle. γ-tubulin Complex Protein 3 (GCP3)-interacting proteins (GIPs) have been identified recently as integral components of γ-TuCs. GIPs have been conserved throughout evolution and are also named MZT1 (mitotic-spindle organizing protein 1). This review focuses on recent data investigating the role of GIP/MZT1 at the NE, including insights from the study of GIP partners. It also uncovers new functions for GIP/MZT1 during interphase and highlights a current view of NE-associated components which are critical for nuclear shaping during both cell division and differentiation.
The nuclear envelope (NE) primarily appeared as a selective barrier between the nucleoplasm and the cytoplasm of Eukaryote cells. However, recent data revealed a rather complex interplay between both compartments, with the NE not only controlling fluxes through nuclear pore complexes (NPCs), but also participating in signal transduction cascades through the establishment of a nucleocytoplasmic continuum (Rothballer and Kutay, 2013). At its outside periphery, the NE allows the anchoring of cytoskeletal proteins and inside, the NE binds to the lamina and chromatin through specific integral membrane proteins. Bridging the inner (INM) and the outer (ONM) nuclear membranes, LInker of the Nucleoskeleton and the Cytoskeleton (LINC) complexes are considered as force transducers between the nuclear lamina and the cytoskeleton (Wang et al., 2012). LINC complexes have been characterized in animal cells (Rothballer and Kutay, 2013) and more recently in plants (Zhou and Meier, 2013). According to cell type specificity, NE composition and function diverge and appear to be critical during differentiation and development (Gomez-Cavazos and Hetzer, 2012).
The LINC complexes are mainly composed of Sad1/UNC84 domain proteins (SUNs) which are generally located at the INM (Tzur et al., 2006), interacting in the perinuclear space with Klarsicht/ANC-1/Syne (KASH) domain proteins anchored at the ONM. In animals, KASH domain proteins connect intermediate filaments, actin, microtubules (MTs), and/or motor proteins (dynein, kinesin). On the nucleoplasmic side, SUNs interact with lamins and/or lamin-associated proteins which constitute the lamina meshwork (Méjat and Misteli, 2010). Closely associated to the INM, the lamina contributes to the mechanical stability and elasticity of the nucleus (Funkhouser et al., 2013). Lamins interact with chromatin and play a role in transcriptional regulation, as well as genome and chromatin organization (Dechat et al., 2008). While SUN proteins have been evolutionary conserved in a remarkable way, KASH proteins have diverged in their sequence and function (Fridkin et al., 2009; Sosa et al., 2013). Altogether, animal LINC complexes are main actors in fundamental functions such as the determination of the NE structure and nuclear shaping, the organization of the cytoskeleton, force transmission at the NE, nuclear anchoring and movement, and genome organization, which leads to controlled gene expression programs (Razafsky and Hodzic, 2009; Starr and Fridolfsson, 2010; Rothballer and Kutay, 2013). It is therefore not surprising that any disruption of these connections causes diverse diseases. Nesprin (KASH domain protein), lamin and lamin-binding protein defects are symptomatic of envelopathies, including muscular dystrophies, cardiomyopathies, and premature aging. At the cellular level, these pathologies are characterized by a lobulation of the nucleus and modifications in the NE structure and its protein organization (Bonne and Quijano-Roy, 2013). It is thus essential – from both a medical and purely biological point of view – to understand how the nuclear shape is regulated and to identify the proteins which contribute to nuclear morphology.
In plant cells, similar nucleocytoplasmic crosstalks have been suspected for a long time, but genetic divergences throughout evolution in LINC complex genes and lamin counterparts delayed their characterization. Nevertheless, the association of several proteins with the nuclear membranes has been described. Among them, the Ran GTPase-activating protein RanGAP1 of Arabidopsis is targeted to the NE through its N-terminal, plant-specific WPP domain (Meier, 2000; Rose and Meier, 2001; Pay et al., 2002). WPP-domain interacting proteins (WIPs) are transmembrane proteins associated with the ONM (Xu et al., 2007), and are the only KASH-like members characterized so far (Meier and Brkljacic, 2009; Zhou et al., 2012). WIPs also interact with WPP domain-Interacting Tail-anchored proteins (WITs) which link the ONM to the actin cytoskeleton through MyosinXI-i (Tamura et al., 2013). SUN proteins are present (Graumann and Evans, 2013; Zhou and Meier, 2013) at the INM where a plant lamina meshwork, called plamina, does also exist (Fiserova et al., 2009; Goldberg, 2013). LITTLE NUCLEI (LINC)/Nuclear Matrix Constituent Proteins (NM) are located in the plamina (Masuda et al., 1997; Dittmer et al., 2007; Kimura et al., 2010), and are good candidates for functional homologues of animal lamins (Ciska and Moreno Diaz de la Espina, 2013; Graumann and Evans, 2013; Sakamoto and Takagi, 2013). However, the connections between INM proteins or plamina components and chromatin have not been described so far.
In the various Eukaryotic cell types, the nuclear surface plays a central role in establishing fully functional MT nucleation and organizing sites. MTs are nucleated from gamma-Tubulin Complexes (γ-TuCs) which are concentrated at the centrosome, positioned close to the nucleus in animal cells, at spindle pole bodies (SPBs) in yeast, or dispersed at various MT nucleating sites located at the plasma membrane, on preexisting MTs and at the NE in plant cells. On the cytoplasmic side, direct links between γ-TuCs and the NE remain poorly understood. Caenorhabditis elegans KASH domain proteins have been reported to participate in centrosome attachment to the NE (Minn et al., 2009) and in Schizosaccharomyces pombe, they link the NE to the SPB where MTs are nucleated (King et al., 2008). In parallel, a novel concept of NE shaping linked to chromatin-bound MT regulation is only just emerging. Developmental pluripotency-associated 2 (Dppa2), a chromatin-binding factor, regulates NE assembly in Xenopus egg extracts through temporally and spatially restricted MT polymerization (Xue et al., 2013). MT dynamics also induce NE and chromatin fiber reshaping in mouse melanoma cells (Gerlitz et al., 2013). In plants, the recent discovery of GIP proteins (Janski et al., 2008, 2012, Nakamura et al., 2012) as integral components of the γ-TuC and the GIP’s partnership (Batzenschlager et al., 2013) tend to ascribe GIP a major role in NE shaping in both cycling and differentiated cells. This review will summarize our current view of a plant nucleocytoplasmic continuum based on genetic and cellular analyses.
Role of GIPs at the ONM in γ-TuC Recruitment and Anchoring
In plants, the whole nuclear surface has been characterized as a MT nucleating site (Erhardt et al., 2002; Seltzer et al., 2003; Canaday et al., 2005; Seltzer et al., 2007); perinuclear MT arrays may connect the NE to the cellular periphery and participate in signal transduction. The γ-tubulin complex proteins GCP2 and GCP3 have been detected at the nuclear periphery (Seltzer et al., 2007). However, no transmembrane domain has been identified within the GCP sequences, indicating that the association of GCPs with the NE requires specific anchoring proteins. In order to identify such components, GCP3 was used as a bait in a yeast two-hybrid screen which allowed the identification of GCP3 Interacting Proteins (GIPs; Janski et al., 2008, 2012). GIPs are small proteins of about 8 kDa encoded by two gene copies in plants. These proteins are mainly structured in three alpha helices (Batzenschlager et al., 2013). GIP1 or GIP2 complemented gip1gip2 knock down mutants at the organism level (Janski et al., 2012), suggesting that both genes mostly shared redundant functions. GCP localization studies performed in gip knock down mutants revealed the role of GIPs in γ-TuC recruitment at the NE and mitotic MT arrays (Janski et al., 2012). In S. pombe, while the GIP homologue Mzt1/Tam4 was dispensable for the assembly of the γ-TuC core, it was required for its recruitment at the SPB (Dhani et al., 2013; Masuda et al., 2013). Therefore, considering their strong conservation throughout evolution, GIPs appear as new actors regulating γ-TuC attachment at MT nucleation sites.
A further yeast two-hybrid screen of an Arabidopsis library, using GIP1 as a bait, led to the identification of TSA1 (At1g52410; Batzenschlager et al., 2013) – earlier described as TonSoku (TSK)-Associating protein 1 (Suzuki et al., 2005). TSA1 is an 84 kDa protein possessing an acidic repeat sequence, rich in alpha helices and coiled-coil domains, and able to mediate the multimerization of the protein. TSA1 also possesses a putative transmembrane domain (Suzuki et al., 2005) and is located, like GIPs, at the NE (Batzenschlager et al., 2013), suggesting its role in anchoring γ-TuCs at the ONM via a GCP3–GIP interaction.
GIP Functions on Both Sides of the NE in Arabidopsis
Confocal observations of the NE region showed that chromocentres, GIPs and MTs were co-aligned (Batzenschlager et al., 2013). At the electron microscopy resolution, GIPs were localized on both sides of the NE and associated with heterochromatin. In addition, gip1gip2 knock down mutants exhibited strongly affected nuclear morphology phenotypes (Janski et al., 2012; Batzenschlager et al., 2013). The flow cytometry analysis of cell DNA contents in these mutants showed that the depletion of GIP proteins led to a significant increase in ploidy levels. This was confirmed by the observation of highly enlarged nuclei in all the tissues analyzed, whether undifferentiated or differentiated. Moreover, the nucleus became lobulated, resembling the phenotypes observed in laminopathies (Mattout et al., 2006). The distribution of NPCs was also affected in gip mutants, showing an increase in NPC density and an NPC misshaping. Simultaneously, the distribution of AtSUN1 became heterogeneous, suggesting a severe modification of the NE organization (Batzenschlager et al., 2013). These nuclear phenotypes are the strongest ever observed in mutated plant cells, suggesting that GIPs play a key role in nuclear shaping and organization, in addition to their role during mitosis (Janski et al., 2012).
Ploidy abnormalities may be the consequence of defects in replication and/or genome maintenance after NE reformation at the chromatin level or in mitosis regulation at the MT level. GIPs are present at the NE during interphase (Figure 1A, arrows), on spindle fibers during mitosis (Figures 1A–Q) and on the reforming NE of daughter nuclei (Figures 1G,I,J,M,N,Q,R, arrows). gip1gip2 mutants show either unstable spindles associated with lagging chromosomes and micronuclei formation or metaphase arrest and polyploid restitution nuclei which may explain both aneu- and polyploid resulting cells (Janski et al., 2012). Similarly, MOZART1, the human GIP homologue (Hutchins et al., 2010; Teixidó-Travesa et al., 2010), is a γ-TuC component whose depletion leads to the accumulation of prometaphase cells with monopolar spindles and closely spaced centrosome pairs (Hutchins et al., 2010), as observed for γ-tubulin (TUBG1) depletion. S. pombe temperature-sensitive or deletion mzt1/Tam4 mutants display defects in MT organization during both interphase and mitosis, showing abnormal anaphases and cytokinesis defects (Dhani et al., 2013; Masuda et al., 2013). These phenotypes suggest a common function of GIPs/MZT1 in mitotic regulation. Furthermore, NE proteins remain present at the spindle matrix during division. Indeed, the INM proteins SUN1 and SUN2 were found in the spindle and close to the spindle poles of tobacco BY-2 and Arabidopsis cells (Graumann and Evans, 2011; Oda and Fukuda, 2011). A localization in the close proximity of chromatin was suggested for SUNs throughout division, but a SUN/chromatin complex has not been identified in plants. Interestingly, TSA1 was also found at the mitotic spindle poles where its partner, the chromatin-remodeling factor TSK, is accumulated (Suzuki et al., 2005). A similar pattern of GIP accumulation was observed within the spindle and at poles in telophase, with GIP1 harboring a punctuated distribution (Figure 1, arrows). This suggests a close connection between GIP and chromatin during NE reformation (Batzenschlager et al., 2013), which may be linked to the nuclear function of TSK in chromatin organization and epigenetic gene silencing (Takeda et al., 2004; Ohno et al., 2011). Ploidy may be controlled at the chromatin level by the coordinated activity of proteins which ensure controlled gene expression and replication, chromatin remodeling and chromosome organization. The presence of GIPs in heterochromatin suggests a role of these proteins in such processes. Unraveling the molecular and genetic relationships between GIP, TSA1 and TSK may clarify this functional aspect.
FIGURE 1. GIP dynamics throughout the cell cycle. Time-lapse images of the expression of pGIP1::AtGIP1-GFP in an Arabidopsis root tip. Three cells were followed for 37 min, using confocal microscopy (A–R). (A) GIP1 localizes in a dotted pattern (arrows) at the nuclear periphery of an interphase cell (a), on a prophase spindle (b) and on a metaphase spindle (c). In end-anaphase-telophase transition, GIP1 remains associated with remnant kinetochore fibers and redistributes in a dotted pattern to the newly built NE (arrows in G, I, J, M and N of the (c) cell and in P–R of the (b) cell). (S) Larger view of a GIP1-GFP expressing root tip in which the nuclear periphery is labeled in interphase and telophase cells (arrows). (T–V) DAPI staining of GIP1–GFP cells confirming that GIP is located at the nuclear periphery. The arrows indicate that some GIP1–GFP signals are located close to chromocentres which are under the inner nuclear membrane. Bar = 10 μm.
Interestingly, the NPC biogenesis is altered in gip mutants which exhibit misshaped NPCs (Batzenschlager et al., 2013). Whether the function of GIP in telophase is required for NPC assembly and NE reformation, which are tightly coordinated processes in metazoans (Güttinger et al., 2009), remains to be determined.
Nuclear Shape Phenotypes in Differentiated/Undifferentiated Cells of Arabidopsis Mutants
In cells lacking the major LINC components characterized so far, defects in the localization of NE-associated proteins and nuclear shaping were often restricted either to the cycling or the differentiated states. Indeed, wip1-1 wip2-1 wip3-1 triple mutants lost the accumulation of RanGAP1 at the NE only in undifferentiated root tip cells of Arabidopsis but did not show peculiar plant growth or NE shape defects. RanGAP1 was also delocalized from the NE in wit1 wit2 double mutant root tips.
Regarding the nuclear shape, the nuclei of trichomes, leaf epidermal cells and mature root hair cells of wip1-1 wip2-1 wip3-1 plants showed a mild change from an elongated to a more roundish form (Zhou et al., 2012), suggesting that other proteins were involved in the control of the nuclear shape. Similarly to wip mutants, the sun1-KO sun2-KD mutant did not show major defects in nuclear positioning or plant development and the nuclei of differentiated cells only became less elongated (Oda and Fukuda, 2011; Zhou et al., 2012). This suggests that defects in plant SUN or KASH domain proteins are less harmful than in their animal SUN-KASH domain counterparts. Concerning plamina components, nmcp1/linc1 and nmcp2/linc2 mutants exhibited a reduced nuclear volume with increasing density of DNA packaging in nuclei of differentiated tissues. However, when expressed under its own promoter, LINC1-GFP preferentially labeled meristematic cell nuclei, but not differentiated root cell nuclei (Dittmer and Richards, 2008). This suggests that in meristematic tissues other NE components may compensate for defects in LINCs/NMCPs for nuclear shaping.
Interestingly, the variations of nucleus morphology described for the wip, sun, and nmcp mutants were observed in differentiated cells (Zhou et al., 2012; Ciska and Moreno Diaz de la Espina, 2013). On the contrary, the dramatic lobulation of gip nuclei was observed in both cycling (root tip) and differentiated cells (cotyledons, leaves and petals; Batzenschlager et al., 2013), suggesting that GIPs were key components involved in the nuclear shape of somatic cells all along the development. Their location at both NE sides and their ability to multimerize are in agreement with (i) their role in γ-TuC recruitment and the regulation of MT array organization and (ii) their interaction with a TSA1–TSK complex, participating in chromatin organization through a nucleocytoplasmic continuum.
Working Model for GIP Functions at the NE
The identification of LINC complex components is emerging in higher plants. At least two types of WIP–SUN complexes are present in Arabidopsis – one which is involved in nuclear shape and movement, including myosin XI-i and WITs (Tamura et al., 2013), and another with RanGAP or WPP proteins for nucleo-cytoplasmic trafficking (Zhou and Meier, 2013; Figure 2A). In addition, other protein complexes may coexist at the NE interface. The model presented in Figures 2B,C illustrates the molecular interplay between GIPs and their identified and possible partners at the NE. WIPs possess a C-terminal PNS tail which terminates in a ϕ-VPT motif (ϕ, hydrophobic amino acid) which shares partial similarity with the PPPX motif of animal KASH domain proteins (Xu et al., 2007). These motifs are essential for interaction with SUN and NE localization of WIP and KASH proteins (Razafsky and Hodzic, 2009; Starr and Fridolfsson, 2010; Zhou et al., 2012). Interestingly, the GIP interacting protein TSA1 harbors a transmembrane domain close to a VIPT motif and is located at the NE (Batzenschlager et al., 2013). Therefore, TSA1 constitutes an attractive candidate for γ-TuC anchoring at the NE in Arabidopsis. The presence of several coiled- coil domains in TSA1 may favor its multimerization as is the case for SUN, KASH (Sosa et al., 2012) and GIP proteins (Batzenschlager et al., 2013). Such multimers may occur between TSA1 or involve additional NE proteins in the perinuclear space. TSA1–TSA1 or TSA1–KASH–SUN complexes may constitute structured platforms, helping γ-TuC attachment and organization. On the nuclear side, LINCs/NMCPs are coiled-coil proteins conserved in multicellular plants (Ciska and Moreno Diaz de la Espina, 2013) and they may interact with SUN proteins (Graumann et al., 2013). Since TSA1 was also shown to interact with the chromatin remodeling factor TSK (Suzuki et al., 2005), TSA1 can possibly locate at both the ONM and the INM. Therefore, a GIP–TSA1 interaction could also occur at the INM interface.
FIGURE 2. Hypothetical model for a new type of nucleocytoplasmic bridges regulating the shape of Arabidopsis thaliana nuclei. (A) A specific plant LINC complex has been characterized, and it plays a role in nuclear movement and shaping (Meier and Brkljacic, 2009; Graumann and Evans, 2010; Zhou et al., 2012; Tamura et al., 2013). Trimeric organization of SUN domain proteins (Sosa et al., 2012) has been omitted for more clarity. A WIT–WIP complex interacts with WWP proteins and RanGAP. WIT also interacts with Myosin XI-i which links the actin cytoskeleton to the ONM. The WIP–SUN complex constitutes the first core LINC identified in plants. NMCPs/LINCs are plamina components (Ciska and Moreno Diaz de la Espina, 2013) which may interact with SUN (Graumann et al., 2013). (B) A new nucleocytoplasmic continuum may consist of GIPs which bind both GCP3, a component of the γ-TuC involved in MT nucleation at the NE, and TSA1, a putative transmembrane protein. TSK localizes in the nucleoplasm (Suzuki et al., 2004; Takeda et al., 2004; Ohno et al., 2011) and may associate with TSA1 at the INM. Both TSK and GIP binding domains of TSA1 are overlapping at its C-terminus. TSA1 possesses coiled-coil domains which may allow multimerization of the protein. Multimerization propensity of GIP/MZT1 proteins have also been reported and could reinforce the interaction with their partners (Batzenschlager et al., 2013; Dhani et al., 2013). Such a model may at least exist in cycling cells. (C) The continuum may also involve a subpopulation of γ-TuCs which does not nucleate MTs in differentiated cells. The GIP–TSA1 complex may interact with a still unknown KASH-like protein associated to SUN in the perinuclear space. The molecular interplay at the INM, plamina and chromatin interface needs to be further characterized.
The loss of the continuum integrity should disturb the forces applied at the NE – originating either from the cytoskeleton and/or the nucleoskeleton – and result in nuclear deformations, as observed in animal cells. This was remarkably observed when GIP genes were mutated in Arabidopsis, and it was probably due to the absence of GIPs on both NE sides. GIP localization studies indicate that GIPs may be involved in molecular links between MT nucleation sites at the ONM and chromocentres at the INM. This is in agreement with the intranuclear localization of GIPs and their possible roles in the intranuclear environment. It may be possible that GIPs regulate chromatin organization and/or gene expression. The phenotypic pleiotropy observed in Arabidopsis gip1gip2 mutants may indicate that these proteins play active roles during the cell cycle for γ-TuC recruitment in close connection with chromatin organization (Batzenschlager et al., 2013).
In the proposed model, γ-TuC recruitment is either linked to MT nucleation (Figure 2B) or does not imply MT assembly. In the latter case, we can consider the existence of a subpopulation of non-nucleating γ-TuCs at the NE (Figure 2C). The presence of such specific γ-TuCs may be linked to differentiation, a status in which nuclei are along a cell edge and perinuclear MT nucleation is switched off. This is in agreement with the absence of nuclear shape alteration in differentiated cells after using MT depolymerization drugs (Sakamoto and Takagi, 2013).
Altogether, considering established and suspected GIP functions, further studies about these small proteins could be of particular interest to decipher physical connections and signaling at the nucleocytoplasmic continuum in plant and other Eukaryotic cells.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by the Centre National de la Recherche Scientifique (CNRS). Morgane Batzenschlager’s PhD was funded by the Ministère de l’Enseignement Supérieur et de la Recherche. The microscopy experiments were carried out using devices from the Strasbourg-Esplanade cellular imaging facilities sponsored by the CNRS, the Université de Strasbourg, and the Région Alsace, the Association de la Recherche sur le Cancer and the Ligue Nationale contre le Cancer (http://ibmp.fr?id=micro). We are very grateful to L. Blech for his valuable advice.
Batzenschlager M., Masoud K., Janski N., Houlné G., Herzog E., Evrard J.-L., et al. (2013). The GIP gamma-tubulin complex-associated proteins are involved in nuclear architecture in Arabidopsis thaliana. Front. Plant Sci. 4:480. doi: 10.3389/fpls.2013.00480
Canaday, J., Brochot, A. L., Seltzer, V., Herzog, E., Evrard, J. L. and Schmit, A. C. (2005) “Microtubule assembly in higher plants,” in Recent Research Developments in Molecular Biology, Vol. 2, ed. S. G. Pandalai (Trivandrum, India: Research Signpost), 103–119.
Dechat, T., Pfleghaar, K., Sengupta, K., Shimi, T., Shumaker, D., Solimando, L., et al. (2008). Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin. Genes Dev. 22, 832–853. doi: 10.1101/gad.1652708
Dhani, D., Goult, B., George, G., Rogerson, D., Bitton, D., Miller, C., et al. (2013). Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3Alp6. Mol. Biol. Cell 24, 3337–3349. doi: 10.1091/mbc.E13-05-0253
Erhardt, M., Stoppin-Mellet, V., Campagne, S., Canaday, J., Mutterer, J., Fabian, T., et al. (2002). The plant Spc98p homologue colocalizes with gamma-tubulin at microtubule nucleation sites and is required for microtubule nucleation. J. Cell Sci. 115, 2423–2431.
Fiserova, J., Kiseleva, E., and Goldberg, M. (2009). Nuclear envelope and nuclear pore complex structure and organization in tobacco BY-2 cells. Plant J. 59, 243–255. doi: 10.1111/j.1365-313X.2009.03865.x
Fridkin, A., Penkner, A., Jantsch, V., and Gruenbaum, Y. (2009). SUN-domain and KASH-domain proteins during development, meiosis and disease. Cell. Mol. Life Sci. 66, 1518–1533. doi: 10.1007/s00018-008-8713-y
Funkhouser, C., Sknepnek, R., Shimi, T., Goldman, A., Goldman, R., and Olvera de la Cruz, M. (2013). Mechanical model of blebbing in nuclear lamin meshworks. Proc. Natl. Acad. Sci. U.S.A. 110, 3248–3253. doi: 10.1073/pnas.1300215110
Hutchins, J., Toyoda, Y., Hegemann, B., Poser, I., Hériché, J.-K., Sykora, M., et al. (2010). Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science 328, 593–599. doi: 10.1126/science.1181348
Janski, N., Herzog, E., and Schmit, A.-C. (2008). Identification of a novel small Arabidopsis protein interacting with gamma-tubulin complex protein 3. Cell Biol. Int. 32, 546–548. doi: 10.1016/j.cellbi.2007.11.006
Janski, N., Masoud, K., Batzenschlager, M., Herzog, E., Evrard, J.-L., Houlné, G., et al. (2012). The GCP3-interacting proteins GIP1 and GIP2 are required for γ-tubulin complex protein localization, spindle integrity, and chromosomal stability. Plant Cell 24, 1171–1187. doi: 10.1105/tpc.111.094904
Kimura, Y., Kuroda, C., and Masuda, K. (2010). Differential nuclear envelope assembly at the end of mitosis in suspension-cultured Apium graveolens cells. Chromosoma 119, 195–204. doi: 10.1007/s00412-009-0248-y
Masuda, H., Mori, R., Yukawa, M., and Toda, T. (2013). Fission yeast MOZART1/Mzt1 is an essential (γ -tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly. Mol. Biol. Cell 24, 2894–2906. doi: 10.1091/mbc.E13-05-0235
Masuda, K., Xu, Z., Takahashi, S., Ito, A., Ono, M., Nomura, K., et al. (1997). Peripheral framework of carrot cell nucleus contains a novel protein predicted to exhibit a long alpha-helical domain. Exp. Cell. Res. 232, 173–181. doi: 10.1006/excr.1997.3531
Minn, I., Rolls, M., Hanna-Rose, W., and Malone, C. (2009). SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional SUN/KASH pair in Caenorhabditis elegans. Mol. Biol. Cell 20, 4586–4595. doi: 10.1091/mbc.E08-10-1034
Nakamura, M., Yagi, N., Kato, T., Fujita, S., Kawashima, N., Ehrhardt, D., et al. (2012). Arabidopsis GCP3-interacting protein 1/MOZART 1 is an integral component of the γ-tubulin-containing microtubule nucleating complex. Plant J. 71, 216–225. doi: 10.1111/j.1365-313X.2012.04988.x
Ohno, Y., Narangajavana, J., Yamamoto, A., Hattori, T., Kagaya, Y., Paszkowski, J., et al. (2011). Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance. Genetics 189, 83–95. doi: 10.1534/genetics.111.130062
Pay, A., Resch, K., Frohnmeyer, H., Fejes, E., Nagy, F., and Nick, P. (2002). Plant RanGAPs are localized at the nuclear envelope in interphase and associated with microtubules in mitotic cells. Plant J. 30, 699–709. doi: 10.1046/j.1365-313X.2002.01324.x
Seltzer, V., Janski, N., Canaday, J., Herzog, E., Erhardt, M., Evrard, J.-L., et al. (2007). Arabidopsis GCP2 and GCP3 are part of a soluble gamma-tubulin complex and have nuclear envelope targeting domains. Plant J. 52, 322–331. doi: 10.1111/j.1365-313X.2007.03240.x
Seltzer, V., Pawlowski, T., Campagne, S., Canaday, J., Erhardt, M., Evrard, J.-L., et al. (2003). Multiple microtubule nucleation sites in higher plants. Cell Biol. Int. 27, 267–269. doi: 10.1016/S1065-6995(02)00345-1
Sosa, B., Rothballer, A., Kutay, U., and Schwartz, T. (2012). LINC complexes form by binding of three KASH peptides to domain interfaces of trimeric SUN proteins. Cell 149, 1035–1047. doi: 10.1016/j.cell.2012.03.046
Starr, D., and Fridolfsson, H. (2010). Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges. Annu. Rev. Cell. Dev. Biol. 26, 421–444. doi: 10.1146/annurev-cellbio-100109-104037
Suzuki, T., Inagaki, S., Nakajima, S., Akashi, T., Ohto, M.-A., Kobayashi, M., et al. (2004). A novel Arabidopsis gene TONSOKU is required for proper cell arrangement in root and shoot apical meristems. Plant J. 38, 673–684. doi: 10.1111/j.1365-313X.2004.02074.x
Suzuki, T., Nakajima, S., Inagaki, S., Hirano-Nakakita, M., Matsuoka, K., Demura, T., et al. (2005). TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis. Plant Cell Physiol. 46, 736–742. doi: 10.1093/pcp/pci082
Takeda, S., Tadele, Z., Hofmann, I., Probst, A. V., Angelis, K. J., Kaya, H., et al. (2004). BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis. Genes Dev. 18, 782–793. doi: 10.1101/gad.295404
Tamura, K., Iwabuchi, K., Fukao, Y., Kondo, M., Okamoto, K., Ueda, H., et al. (2013). Myosin XI-i links the nuclear membrane to the cytoskeleton to control nuclear movement and shape in Arabidopsis. Curr. Biol. 23, 1776–1781. doi: 10.1016/j.cub.2013.07.035
Teixidó-Travesa, N., Villen, J., Lacasa, C., Bertran, M. T., Archinti, M. A., Gygi, C., et al. (2010). The gammaTuRC revisited: a comparative analysis of interphase and mitotic human gamma TuRC redefines the set of core components and identifies the novel subunit GCP8. Mol. Biol. Cell 21, 3963–3972. doi: 10.1091/mbc.E10-05-0408
Xu, X. M., Meulia, T., and Meier, I. (2007). Anchorage of plant RanGAP to the nuclear envelope involves novel nuclear-pore-associated proteins. Curr. Biol. 17, 1157–1163. doi: 10.1016/j.cub.2007.05.076
Xue, J., Woo, E., Postow, L., Chait, B., and Funabiki, H. (2013). Chromatin-bound Xenopus dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Dev. Cell 27, 47–59. doi: 10.1016/j.devcel.2013.08.002
Zhou, X., Graumann, K., Evans, D., and Meier, I. (2012). Novel plant SUN-KASH bridges are involved in RanGAP anchoring and nuclear shape determination. J. Cell Biol. 196, 203–211. doi: 10.1083/jcb.201108098
Keywords: nucleocytoplasmic continuum, gamma-tubulin complex, spindle assembly, nuclear envelope proteins, Arabidopsis thaliana
Citation: Batzenschlager M, Herzog E, Houlné G, Schmit A-C and Chabouté M-E (2014) GIP/MZT1 proteins orchestrate nuclear shaping. Front. Plant Sci. 5:29. doi: 10.3389/fpls.2014.00029
Received: 22 November 2013; Paper pending published: 11 December 2013;
Accepted: 22 January 2014; Published online: 07 February 2014.
Edited by:Katja Graumann, Oxford Brookes University, UK
Reviewed by:Daniel A. Starr, University of California, Davis, USA
David Edgar Evans, Oxford Brookes University, UK
Copyright © 2014 Batzenschlager, Herzog, Houlné, Schmit and Chabouté. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Anne-Catherine Schmit, Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, 12 rue du Gl Zimmer, 67084 Strasbourg, France e-mail: firstname.lastname@example.org