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Front. Plant Sci. | doi: 10.3389/fpls.2018.00269

Expression patterns and identified protein-protein interactions suggest that cassava CBL-CIPK signal networks function in responses to abiotic stresses

  • 1Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/ Institute of Tropical Agriculture and Forestry, Hainan University, China

Cassava is an energy crop that is tolerant of multiple abiotic stresses. It has been reported that the interaction between Calcineurin B-like (CBL) protein and CBL-interacting protein kinase (CIPK) is implicated in plant development and responses to various stresses. However, little is known about their functions in cassava. Herein, 8 CBL (MeCBL) and 26 CIPK (MeCIPK) genes were isolated from cassava by genome searching and cloning of cDNA sequences of Arabidopsis CBLs and CIPKs. Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis showed that the expression levels of MeCBL and MeCIPK genes were different in different tissues throughout the life cycle. The expression patterns of 7 CBL and 26 CIPK genes in response to NaCl, PEG, heat and cold stresses were analyzed by quantitative real-time PCR (qRT-PCR), and it was found that the expression of each was induced by multiple stimuli. Furthermore, we found that many pairs of CBLs and CIPKs could interact with each other via investigating the interactions between 8 CBL and 25 CIPK proteins using a yeast two-hybrid system. Yeast cells co-transformed with cassava MeCIPK24, MeCBL10, and Na+/H+ antiporter MeSOS1 genes exhibited higher salt tolerance compared to those with one or two genes. These results suggest that the cassava CBL-CIPK signal network might play key roles in response to abiotic stresses.

Keywords: Calcineurin B-like protein, CBL-interacting protein kinase, abiotic stress, Signal pathway, cassava

Received: 07 Nov 2017; Accepted: 14 Feb 2018.

Edited by:

Nicholas Provart, University of Toronto, Canada

Reviewed by:

Fouad Lemtiri-Chlieh, King Abdullah University of Science and Technology, Saudi Arabia
David M. Rhoads, California State University, San Bernardino, United States  

Copyright: © 2018 Mo, Wan, Xia, Ren, Zhou and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xingyu Jiang, Hainan University, Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/ Institute of Tropical Agriculture and Forestry, Haikou, 570228, China, jiangxingyu2002@yahoo.com