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Front. Plant Sci. | doi: 10.3389/fpls.2018.00405

Mutation in Rice Abscisic Acid2 Results in Cell Death, Enhanced Disease-resistance, Altered Seed Dormancy and Development

 Liao Yongxiang1*, Bai Que1, Xu Peizhou1, Wu Tingkai1,  Guo Daiming1, Peng Yongbin1,  ZhangHongyu Hongyu1, Deng Xiaoshu1, Chen Xiaoqiong1, Luo Ming2,  Ali Asif1, Wang Wenming1 and Wu Xianjun1
  • 1Sichuan Agricultural University, China
  • 2Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia

Lesion mimic mutants display spontaneous cell death, and thus are valuable for understanding the molecular mechanism of cell death and disease-resistance. Although a lot of such mutants have been characterized in rice, the relationship between lesion formation and abscisic acid (ABA) synthesis pathway is not reported. In the present study, we identified a rice mutant, lesion mimic mutant9150 (lmm9150), exhibiting spontaneous cell death, pre-harvest sprouting, enhanced growth and resistance to rice bacterial and blast diseases. Cell death in the mutant was accompanied with excessive accumulation of H2O2. Enhanced disease-resistance was associated with cell death and up-regulation of defense-related genes. Map-based cloning identified a G-to-A point mutation resulting in a D-to-N substitution at the amino acid position 110 of OsABA2 (LOC_Os03g59610) in lmm9150. Knock-out of OsABA2 through CRISPR/Cas9 led to phenotypes similar to those of lmm9150. Consistent with the function of OsABA2 in ABA biosynthesis, ABA level in the lmm9150 mutant was significantly reduced. Moreover, exogenous application of ABA could rescue all the mutant phenotypes of lmm9150. Taken together, our data linked ABA deficiency to cell death and provided insight into the role of ABA in rice disease-resistance.

Keywords: Abscisic Acid, xanthoxin dehydrogenase, gibberellin, ABA/GA ratio, lesion mimic mutant, Pre-harvest sprouting, defense responses, Oryza sativa L

Received: 09 Nov 2017; Accepted: 14 Mar 2018.

Edited by:

Zhengqing Fu, University of South Carolina, Columbia, United States

Reviewed by:

MARINA GAVILANES-RUIZ, Universidad Nacional Autónoma de México, Mexico
Aardra Kachroo, University of Kentucky, United States  

Copyright: © 2018 Yongxiang, Que, Peizhou, Tingkai, Daiming, Yongbin, Hongyu, Xiaoshu, Xiaoqiong, Ming, Asif, Wenming and Xianjun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Liao Yongxiang, Sichuan Agricultural University, Ya'an, China,