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Front. Plant Sci. | doi: 10.3389/fpls.2018.00544

The two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, are negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea

  • 1Shenyang Agricultural University, China
  • 2Liaoning Academy of Agricultural Sciences, China

Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea. Subcellular localization analysis showed that CsTCTP1 and CsTCTP2 were both localized in the cytoplasm. Expression analysis indicated that the transcript levels of CsTCTP1 and CsTCTP2 were linked to the degree of cucumber resistance to S. fuliginea. Transient overexpression of either CsTCTP1 or CsTCTP2 in cucumber cotyledons impaired resistance to S. fuliginea, whereas silencing of either CsTCTP1 or CsTCTP2 enhanced cucumber resistance to S. fuliginea. The relationship of several defense-related genes and ABA and target of rapamycin (TOR) signaling pathway-related genes to the overexpressing and silencing of CsTCTP1/CsTCTP2 in non-infested cucumber plants was investigated. The results indicated that CsTCTP1 participates in the defense response to S. fuliginea by regulating the expression of certain defense-associated genes and/or ABA signaling pathway-associated genes, and CsTCTP2 participates through regulating the expression of TOR signaling pathway-associated genes. Our findings will guide enhancing the resistance of cucumber to powdery mildew.

Keywords: ABA signaling pathway, Cucumis sativus, Sphaerotheca fuliginea, Translationally Controlled Tumor Protein, Transient transformation, TOR signaling pathway

Received: 24 Jan 2018; Accepted: 09 Apr 2018.

Edited by:

Essaid Ait Barka, Université de Reims Champagne Ardenne, France

Reviewed by:

ISABEL DIAZ, Universidad Politécnica de Madrid (UPM), Spain
MOSTAFA A. ABDELRAHMAN, Tottori University, Japan  

Copyright: © 2018 Meng, Yu, Zhao, Cui, Song, Yang and Fan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Hai y. Fan, Shenyang Agricultural University, Shenyang, China, Hyfan74@163.com