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Front. Plant Sci. | doi: 10.3389/fpls.2018.00577

Lack of a cytoplasmic RLK, required for ROS homeostasis, induces strong resistance to bacterial leaf blight in rice

  • 1Graduate School of Biotechnology, Kyung Hee University, South Korea
  • 2Max Planck Institut für Pflanzenzüchtungsforschung, Germany
  • 3Seoul National University, South Korea

Many scientific findings have been reported on the beneficial function of reactive oxygen species (ROS) in various cellular processes, showing that they are not just toxic byproducts. The double-edged role of ROS shows the importance of the regulation of ROS level. We report a gene, rrsRLK (required for ROS-scavenging receptor-like kinase), that encodes a cytoplasmic RLK belonging to the non-RD kinase family. The gene was identified by screening rice RLK mutant lines infected with Xanthomonas oryzae pv. oryzae (Xoo), an agent of bacterial leaf blight of rice. The mutant (ΔrrsRLK) lacking the Os01g02290 gene was strongly resistant to many Xoo strains, but not to the fungal pathogen Magnaporthe grisea. ΔrrsRLK showed significantly higher expression of OsPR1a, OsPR1b, OsLOX, RBBTI4, and jasmonic acid-related genes than wild type. We showed that rrsRLK protein interacts with OsVOZ1 (vascular one zinc-finger 1) and OsPEX11 (peroxisomal biogenesis factor 11). In the further experiments, abnormal biogenesis of peroxisomes, hydrogen peroxide (H2O2) accumulation, and reduction of activity of ROS-scavenging enzymes were investigated in ΔrrsRLK. These results suggest that the enhanced resistance in ΔrrsRLK is due to H2O2 accumulation caused by irregular ROS-scavenging mechanism, and rrsRLK is most likely a key regulator required for ROS homeostasis in rice.

Keywords: OsPEX11 (rice peroxisomal biogenesis factor 11), OsVOZ1 (rice vascular one zinc-finger 1), plant defense, Reactive Oxygen Species, ROS-homeostasis, Xanthomonas oryzae pv. oryzae

Received: 12 Feb 2018; Accepted: 13 Apr 2018.

Edited by:

Víctor Flors, Jaume I University, Spain

Reviewed by:

Lucía Jordá, Universidad Politécnica de Madrid (UPM), Spain
Kaijun Zhao, Institute of Crop Science, Chinese Academy of Agricultural Sciences  

Copyright: © 2018 Yoo, Park, Cho, Yang, Kim, Jung, Jeon, An and Lee. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Sang-Won Lee, Graduate School of Biotechnology, Kyung Hee University, Yongin, South Korea,