Impact Factor 3.677
2017 JCR, Clarivate Analytics 2018

The world's most-cited Plant Sciences journal

This article is part of the Research Topic

Biostimulants in Agriculture

Original Research ARTICLE

Front. Plant Sci., 01 February 2019 | https://doi.org/10.3389/fpls.2018.01959

Exposure in vitro to an Environmentally Isolated Strain TC09 of Cladosporium sphaerospermum Triggers Plant Growth Promotion, Early Flowering, and Fruit Yield Increase

Zhijian T. Li1, Wojciech J. Janisiewicz1, Zongrang Liu1, Ann M. Callahan1, Breyn E. Evans1, Wayne M. Jurick II2 and Chris Dardick1*
  • 1Appalachian Fruit Research Station, United States Department of Agriculture – Agricultural Research Service, Kearneysville, WV, United States
  • 2Food Quality Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture – Agricultural Research Service, Beltsville, MD, United States

A growing number of bacteria and fungi have been found to promote plant growth through mutualistic interactions involving elements such as volatile organic compounds (VOCs). Here, we report the identification of an environmentally isolated strain of Cladosporium sphaerospermum (herein named TC09), that substantially enhances plant growth after exposure in vitro beyond what has previously been reported. When cultured on Murashige and Skoog (MS) medium under in vitro conditions, tobacco seedlings (Nicotiana tabacum) exposed to TC09 cultures for 20 days increased stem height and whole plant biomass up to 25- and 15-fold, respectively, over controls without exposure. TC09-mediated growth promotion required >5 g/L sucrose in the plant culture medium and was influenced by the duration of exposure ranging from one to 10 days, beyond which no differences were detected. When transplanted to soil under greenhouse conditions, TC09-exposed tobacco plants retained higher rates of growth. Comparative transcriptome analyses using tobacco seedlings exposed to TC09 for 10 days uncovered differentially expressed genes (DEGs) associated with diverse biological processes including cell expansion and cell cycle, photosynthesis, phytohormone homeostasis and defense responses. To test the potential efficacy of TC09-mediated growth promotion on agricultural productivity, pepper plants (Capsicum annuum L.) of two different varieties, Cayenne and Minisweet, were pre-exposed to TC09 and planted in the greenhouse to monitor growth, flowering, and fruit production. Results showed that treated pepper plants flowered 20 days earlier and yielded up to 213% more fruit than untreated controls. Altogether the data suggest that exposure of young plants to C. sphaerospermum produced VOCs may provide a useful tool to improve crop productivity.

Introduction

Innumerable bacterial and fungal microorganisms colonize various habitats and can influence the survival of plants. Many such microorganisms promote plant growth through mutualistic interactions which have been the subject of intensive studies for more than 150 years (Whipps, 2001; Berg, 2009). In general, beneficial microorganisms are categorized into a number of groups depending on their habitats and functional roles. They include organic matter decomposers, nitrogen fixing bacteria, mycorrhizal fungi, detoxifiers, mutualistic endophytes, and pathogen-antagonists (Barea et al., 2005). These microbes produce and release unique proteins, enzymes, antibiotics, phenolics, lipids, carbohydrates, and phytohormones improving the availability of essential nutrients and plant growth. In addition, they are capable of activating plant defenses and protecting against biotic and abiotic stresses (Wardle et al., 2004; Barea et al., 2005; Saharan and Nehra, 2011; De-la-Peña and Loyola-Vargas, 2014). Over the past several decades, concerted efforts have focused on the study of plant–microbe symbiotic relationships and endophytic interactions to elucidate plant growth promoting (PGP) activities of beneficial microorganisms (Barea et al., 2005; Berg and Smalla, 2009; Lambers et al., 2009; Saharan and Nehra, 2011; Ahemad and Kibret, 2014; De-la-Peña and Loyola-Vargas, 2014). Consequently, a wide range of microbial inoculants and biocontrol products were developed and marketed as biopesticides, plant strengtheners, phytostimulators, and biofertilizers to improve soil conditions, increase crop productivity and control soil borne pests while reducing reliance on agrochemicals. These products have the potential to encourage environmentally friendly and sustainable cultivation practices and protect biodiversity (Thakore, 2006; Berg, 2009; Baez-Rogelio et al., 2016).

The utilization of current microbial products designed to be applied to the rhizosphere or as inoculants for PGP commonly suffer from unpredictability and inconsistency (Berg, 2009; Ahemad and Kibret, 2014). Changes in soil conditions due to tillage and erosion, extreme weather events and capricious mutualistic interactions amongst microorganisms outside or within host plants can influence the biochemical, physiological and metabolic activities of microbial community and the survival of beneficial microorganisms (De Souza et al., 2015; Baez-Rogelio et al., 2016). A number of studies revealed that some beneficial microorganisms under certain growth and environmental conditions can also produce phytotoxic substances such as hydrogen cyanide (HCN) and other nitrogen and sulfur compounds that can adversely affect plant growth (Kremer and Souissi, 2001; Weise et al., 2013; Nadeem et al., 2014). Clearly, a better understanding of the responses of beneficial microorganisms to their environments will help the development of sustainable strategies that can maximize the effectiveness of microbial products and minimize the deleterious effects on plant growth.

Recently, research activities in the utilization of microorganisms that enhance plant growth via microbial volatile organic compounds (MVOCs) have been intensified based on the premise that they might provide an eco-friendly, cost-effective and sustainable strategy to benefit agriculture (Kanchiswamy et al., 2015a,b; Li et al., 2016). MVOCs are small (<300 Da) and vaporous/gaseous semiochemicals with relatively high vapor pressures, low boiling points and high levels of lipophilicity (Schulz and Dickschat, 2007; Lemfack et al., 2014). These compounds are mostly organic/hydrocarbon in nature (Audrain et al., 2015). Microorganisms from many bacterial and fungal species and genera are able to synthesize a plethora of MVOCs with various biological functions. According to a recent tally, 400 out of 10,000 microorganisms have been described for their ability to produce up to 1,000 MVOCs (Schulz and Dickschat, 2007; Lemfack et al., 2014; Peñuelas et al., 2014; Kanchiswamy et al., 2015b; Li et al., 2016). However, among the complex blends of MVOCs, only a handful have been demonstrated to function in PGP. For instance, 141 MVOCs were detected from 20 strains and 11 species of Trichoderma examined. Yet, only 18 compounds or 11% of the detectable pool were able to promote plant growth, whereas the remaining majority compounds were either neutral or inhibitory to plant growth (Lee et al., 2016). In some cases, phytopathogenic microorganisms are also capable of emitting PGP MVOCs but their positive activities are often subdued by strong phytotoxic substances and parasitic or pathogenic behaviors (Sánchez-López et al., 2016b; Cordovez et al., 2017). While specific correlation between microbe-derived volatiles and plant responses requires further examination and the mechanisms of action remain unknown in most cases, a growing number of studies have linked PGP MVOCs to diverse mechanisms. These include regulation of photosynthesis, starch accumulation, phytohormone homeostasis/signaling and cell expansion, activation of innate immunity and abiotic stress responses, reprogramming of developmental controls for promoted reproductive growth, and even altering the behaviors of other microorganisms through inter- and intra- kingdom microbial interactions (Ryu et al., 2003, 2004; Zhang et al., 2007; Minerdi et al., 2011; Pieterse et al., 2014; Bitas et al., 2015; Sánchez-López et al., 2016a,b; Cordovez et al., 2017; Farag et al., 2017; Tahir et al., 2017).

Despite these studies, challenges remain in applying MVOC-mediated PGP technology to crop production. More research is needed to identify and fully exploit novel MVOC-emitting microorganisms and develop effective deployment strategies (Ahmad et al., 2008; Berg, 2009; Kanchiswamy et al., 2015a; Turner and Meadows-Smith, 2016). In this study, we report on the discovery of a non-pathogenic airborne fungus Cladosporium sphaerospermum, strain TC09 that strongly stimulates plant growth through exposure in vitro. By using tobacco (Nicotiana tabacum) and pepper (Capsicum annuum) as models, conditions for fungal exposure and fungal cultivation to achieve optimal plant growth stimulation were investigated. We demonstrated growth stimulation, early flowering and fruit yield increases following a relatively short duration of in vitro MVOC exposure at seedling stage. Comparative transcriptome analysis was conducted to reveal differential gene expression associated with promoted plant growth and development. PGP activities following in vitro exposure using related fungal species and isolates were also compared.

Materials and Methods

Culture Media

Premixed medium powder containing basal salts and vitamins of MS medium (Murashige and Skoog, 1962) were purchased from PhytoTechnology Laboratories (Cat No. M519, Overland Park, KS, United States). For in vitro culture of plants, full strength MS medium supplemented with 30 g/L sucrose (Sigma-Aldrich, St. Louis, MO, United States, S5391) was prepared with pH adjusted to 5.8 using 1N KOH prior to addition of 7 g/L gelling agar (Sigma, A7921). The medium was then autoclaved at 121°C for 20 min and cooled down to 45°C prior to dispensing to Magenta GA7 vessels (100 ml per vessel) and 15 × 100 mm Petri dishes (30 ml/Plate).

Species Identification of TC09

TC09 was an environmentally isolated strain of unknown fungus and was subsequently deposited at the Agricultural Research Service Culture Collection (NRRL) with an assigned #: NRRL 67603. To determine the species identity, single spore cultures were transferred to MS medium in Petri plates and incubated at 25°C. Fungal spores were collected from the cultures and kept in a 1.5 ml microcentrifuge tube at -20°C. Genomic DNA was isolated using the DNEasy Plant Mini Kit (Qiagen, Hilden, Germany). Briefly, conidia were removed from the freezer and liquid nitrogen was added to the microcentrifuge tube. The conidia were ground to a fine powder using a motorized pestle mixer (VWR Pellet Mixer, VWR, Intl., Radnor, PA, United States). The DNA was isolated following the manufacturer’s protocol with one exception; in the final step, DNA was eluted from the spin column using 100 μL hot (65°C) nuclease-free water. DNA concentration was determined using a Qubit® 2.0 fluorometer and the dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, United States). Polymerase chain reaction (PCR) was performed with the genomic DNA as a template. The internal transcribed spacer 1 (ITS1) and ITS2 regions associated with the 5.8S ribosomal RNA (rRNA) gene in fungal organisms were targeted for species identification. Two reactions using primer pairs ITS1/2 and ITS3/4, respectively, were conducted in a Bio-Rad thermocycler using 30 reaction cycles each consisting of 94°C for 1 min, 60°C for 1 min, and 72°C for 3 min. Sequences of the primer pairs are as described by White et al. (1990) (Table 1). Both amplicons were visualized on a 0.8% agarose gel stained with ethidium bromide (EtBr) following electrophoresis. DNA products in single bands were purified from isolated gel blocks using Qiagen PCR Clean Up kit and DNA concentrations were quantified using a Nanodrop spectrophotometer. Purified DNA Products were subject to Sanger sequencing by Eurofins MWG Operon (KY, United States) and sequence data were analyzed using Geneious software (Biomatters Inc., Newark, NJ, United States).

TABLE 1
www.frontiersin.org

Table 1. Oligonucleotide primers and amplicons targeting the internal transcribed spacer 1 (ITS1) and ITS2 regions associated with the 5.8S ribosomal RNA (rRNA) gene in fungi.

To determine phylogenetic relationships, DNA sequences of 145 related fungal species/isolates with over 95% homology to the consensus sequence of the ITS3/4 primer pair-amplified PCR products were retrieved via a BLAST search of GenBank database. The sequences were aligned and analyzed using default settings of the Phylogeny Module of CLC Genomics Workbench software program (Version 10, Qiagen, Redwood City, CA, United States) with Neighbor-Joining method with 1,000 bootstrap replicates.

For microscopic examination, TC09 was grown on PDA (Potato dextrose agar) or MS media for 7–10 days at 22°C under continuous light. Squares of transparent adhesive tape (Scotch Magic tape, 3M, St. Paul, MN, United States) were gently placed along the edge of the colony with forceps. Tape squares were removed from the colony margin and stained for 20 min with 1% aqueous Calcofluor white M2R (Fluorescent brightener 28, Sigma, St. Louis, MO, United States). Tape squares were gently rinsed in sterile distilled water and mounted between drops of 50% glycerol under a glass cover slip. The cover slip was affixed in place using clear nail polish. Mounted specimens were visualized through confocal microscopy (Zeiss LSM-800, Carl Zeiss AG, Oberkochen, Germany) and images were captured using the manufacturer software.

Assay of TC09 Promotion Activities on Tobacco Plant Growth

Tobacco seeds (Nicotiana tabacum cv. “Samsun”) were surface-sterilized by rinsing briefly in 95% ethanol and then immersed in 20% (v/v) bleach containing 8.25% w/v sodium hypochlorite with constant agitation for 10 min. After 3× rinse with sterile water, seeds were then spread evenly on the surface of MS medium in Petri plates. The plates were sealed with plastic wrap and maintained at 25°C under 16-h light (50 μmol m2 s1) and 8-h dark photoperiods for 6 days. Seedlings with fully expanded cotyledons were selected based on a uniform plant size and an appearance free of abnormality (∼80–90% of sowed seeds).

TC09 inoculant in aqueous conidial suspension was prepared by first culturing the fungal conidia on MS plate for 1 week followed by collecting conidia in sterile 0.01% Triton X-100/water solution and adjusting density to 105 conidia per ml prior to use or storage at -80°C. For initial experiments, fungal cultures grown in Eppendorf tubes were placed inside the plant tissue culture vessel but physically separated using sealed biological filters that restrict the movement of conidia but not volatiles. First, aliquots of 300 μl warm MS medium were poured into 1.5 ml Eppendorf microcentrifuge tubes. Tubes were positioned diagonally to form a slant surface while cooling in laminar flow hood. Ten microliters of an aqueous conidial suspension of TC09 was transferred onto the surface of the medium in each tube and thus inoculated tubes were plugged with a sterile aerosol substance- and liquid-resistant filter (Rainin #17001945, Mettler Toledo, Oakland, CA, United States). Two tubes were directly inserted at separate corners of a Magenta GA7 vessel containing three tobacco seedlings each. The vessels were then sealed with plastic wrap and maintained under light conditions with a 16-h photoperiod at 25°C. Plant growth was monitored and compared to controls grown without fungal cultures.

In subsequent experiments open-end culture tube closures (Sigma C5791) were used to reduce premature build-up of condensation water on the surface of the fungal mycelium as found in above microcentrifuge tube setting. Each sterile closure contained 5 ml semi-solid MS medium followed by addition of 10 μl of TC09 suspension as inoculant. One inoculated closure was then placed in each Magenta GA7 vessel that contained three tobacco seedlings for fungal exposure treatment. For controls, a MS-containing closure without fungal inoculant was added. Vessels were placed under above-mentioned light conditions at 25°C. To measure plant growth promotion under in vitro conditions, a 20-day exposure duration was employed which was comparable to previous studies using tobacco (Paul and Park, 2013; Lee et al., 2016).

Three plants were grown in each replicate vessel and three vessels were used per treatment. The experiment was conducted three times unless otherwise stated. Plant growth was determined by measurement of major growth parameters including total plant height, total plant fresh weight, stem length, number of traceable leaves, root system length, and length of largest leaf. Data were analyzed by using SAS statistics program with standard statistical approaches including standard error, t-test (two means), ANOVA and Tukey’s HSD mean separation test (multiple means) with a statistical significance level P < 0.05.

Effect of TC09 Exposure Duration and Sugar Concentration

For experiments to test the effect of exposure time under in vitro conditions, control tube closures with medium-only (CK) and those with fungal cultures were placed in vessels containing tobacco seedlings. Fungal cultures were then removed after an incrementally extended exposure duration from 1, 4, 10, or 20 days. Vessels containing medium only were treated as 0 day and served as a control. All plants were allowed to grow continuously in the vessels until 20 days after introduction of fungal cultures when growth was measured. The experiment was conducted two times.

A total of six sucrose concentrations including 0, 5, 10, 20, 30, and 40 g/L were incorporated in MS medium and used to culture tobacco seedlings. Introduction of TC09 cultures in tube closures to Magenta GA7 vessels was carried out as aforementioned. After a 20-day fungal exposure, plants were collected, measured and analyzed as described above. Experiments were conducted two times.

Plant Growth Under ex vitro/Soil Conditions in the Greenhouse

In vitro grown plants following fungal exposure were transplanted to potting soil mix (Metro Mix 360, Sun Gro Horticulture, Canada) and maintained in a greenhouse to monitor growth. Soil mix was pre-sterilized by autoclaving at 121°C for 90 min prior to use. Plants were maintained in a greenhouse. Six to ten individual tobacco plants from each treatment and untreated controls were transplanted for each test and experiments were conducted three times. Plant growth and development were evaluated via periodic measurement of plant height and total leaf numbers over the course of 70 days beginning from the time the seeds were sown. Experimental data were analyzed using standard statistical approaches as mentioned above.

Effect of TC09 Growth Media on Plant Growth Promotion

A total of six media were examined for fungal cultivation including MS (Murashige and Skoog, 1962), PDA (potato dextrose agar), Czapek (CYA, Czapek-DOX Yeast agar), Malt (Malt extract agar), yeast (Yeast extract extract) and Hutner’s medium. Formula and preparation procedures for the last five culture media were described previously (Sinclair and Dhingra, 1995). Organic potatoes were purchased locally and used to prepare potato infusion and PDA medium. For these experiments, tobacco and closure-mediated method for fungal exposure were employed. Final determination of plant growth was made at the end of an exposure duration of 20 days. There were three plants per replicate vessel and three vessels per treatment. The experiment was repeated twice.

Effect of TC09-Related Fungal Species/Isolates on Plant Growth Promotion

Comparative assays were performed to determine the effectiveness of exposure in vitro using various species and isolates belonging to Cladosporium that were kindly provided by Dr. Frank M. Dugan of USDA (59 Johnson Hall, WSU, Pullman, WA, United States). These include C. sphaerospermum NRRL8131, C. cladosporioides 113d, C. asperulatum 208db, C. subtilissimum WF99-209, C. cladosporioides W99-175a, and C. macrocarpum Clad ex Phyl 8. Tobacco plants were used for growth comparison using MS medium for fungal culture. Three replicate vessels with three plants each were used for each treatment. The experiment was repeated twice.

Comparative Transcriptome Analysis of Tobacco Plants Following Exposure to TC09

Transcriptome analysis was carried out using total RNA isolated from tobacco seedlings grown under in vitro conditions with or without TC09 exposure for 10 days. For RNA isolation, shoot tips carrying the apical meristem and three to four terminal leaves were collected from each replicate plant, placed in 1.5 ml microcentrifuge tubes, and immediately immersed in liquid nitrogen. Samples were pulverized into a fine powder using Geno/Grinder 2010 (SPEX Sample Prep, Metuchen, NJ, United States). Total RNA was then extracted from 100 mg tissue powder using the RNeasy® Plant Mini Kit per manufacturer’s instructions (Qiagen, Redwood City, CA, United States). There were four biological replicates from each treatment. RNA sequencing was performed by Genewiz via Illumina HiSeq platform (50 bp single-end reads) (South Plainfield, NJ, United States). Raw sequences were paired, trimmed and filtered to obtain quality reads. Reads were mapped to the Solanum lycopersicum reference genome with CLC Genomics Workbench (version 10, Qiagen, Redwood City, CA, United States) using default parameters (The Tomato Genome Consortium, 2012). Differentially expressed genes (DEGs) were identified using false discovery rate (FDR) ≤ 0.05. Genes were annotated per referenced tomato (S. lycopersicum) transcriptome dataset available on https://solgenomics.net and associated gene ontology (GO) terms. The RNAseq data was deposited to NCBI GEO under reference ID GSE120288.

Effect of TC09 Exposure in vitro on Pepper Plant Growth and Production

Two different varieties of pepper (Capsicum annuum), cayenne pepper ‘Long Thin’ and sweet bell pepper ‘Minisweet Pepper Mixed,’ were tested to determine growth response to TC09 exposure in vitro. Pepper seeds were acquired from commercial source (Plantation Product LLC., Norton, MA, United States) and sterilized by previously described method prior to in vitro cultivation or seeded directly in the soil as controls. Germinated pepper seedlings were exposed to closure-contained TC09 while culturing on MS medium in GA7 vessels for 20 days and then transplanted to soil in 8-inch pots. Plants were maintained in the greenhouse using standard management practice. Plant growth and fruit production were monitored continuously until fruit ripening. In these experiments each treatment consisted of four to five individual plants. Experiments were conducted two times each in a similar seasonal span from late winter to early spring in 2017 and 2018, respectively.

Results

Isolation and Species Identification of TC09 Fungus

An airborne fungal contaminant on a tissue culture plate containing tobacco seedlings growing on MS medium was isolated and single-spore purified based on the preliminary visual observation that plants in the contaminated plate grew much larger than comparable plants on uncontaminated plates. To identify the species, DNA was extracted and the internal transcribed spacer was amplified by PCR and subsequently sequenced. The sequence of the two fragments generated from the primer pairs are specified in Table 1.

Upon comparison with existing sequences, the 138 bp ITS1/2 amplicon was 100% identical to a GenBank accession KU926349.1 belonging to Cladosporium sphaerospermum isolate UACH-124. The 249 bp ITS3/4 amplicon was 100% identical to a GenBank accession KX982238.1 of Cladosporium sphaerospermum strain 7. Phylogenetic analysis of 148 sequences homologous to the ITS3/4 amplicon that were available in GenBank showed that TC09 clusters with known isolates of C. sphaerospermum (Supplementary Figure S1).

To confirm the results of genetic identification, TC09 was cultured on PDA medium, which is commonly used as a standard condition for fungal species identification. TC09 colonies grew to 20 mm and 35 mm in diameter during 7 and 14 days, respectively, on PDA at 20°C.=. Colonies were olivaceous-gray in the center and olivaceous to iron-gray in the outer region (Supplementary Figure S3A). On the reverse they were iron-gray and turned almost blackish at 14 days. Conidiophores were not much differentiated, occasionally branched with conidia produced in branching chains with variable shapes with smaller size toward the apex (Supplementary Figures S3B,C). Intercalary conidia were 1.7–3.2 × 2.4–8.0 μm and terminal conidia 1.9–2.5 × 2.1–2.9 μm. Hyphae was 2.6–3.2 μm wide, sparsely to profusely branched at 45–90° angles, distinctly septate with cell length averaging 17.5 μm and ranging from 12.4 to 27.3 μm (Supplementary Figure S3D). Collectively, the morphological results were consistent with TC09 being an isolate of C. sphaerospermum as described by Dugan et al. (2008) and Ababutain (2013).

Stimulation of Plant Growth Is due to in vitro Exposure to TC09

Since the fungal mycelium and tobacco plants appeared to be physically separated in the original contaminated culture plate, possible involvement of MVOCs in plant growth promotion was investigated. Tobacco plants were tested with and without the inclusion of biological filter-sealed Eppendorf tubes containing TC09 in Magenta culture vessels. In this manner, the plants were exposed only to volatiles produced by the fungus. Visual observation indicated that plants with fungal exposure for 10 days exhibited more vigorous growth and thicker stems, larger leaves, and a more robust root system relative to plants without fungal exposure (Figure 1A). No conidia from the filter-sealed Eppendorf tubes were visually detected in either the culture vessel headspace or plant culture medium.

FIGURE 1
www.frontiersin.org

Figure 1. Growth promotion of tobacco by exposure to TC09. (A) Control tobacco plants without fungal exposure (left) and plants exposed to physically separated TC09 contained in filter-sealed microcentrifuge tubes (right). Arrows indicated the fungus with dark-colored mycelium. Photograph was taken 10 days after fungal exposure. (B) Tobacco plants exposed to TC09 contained in open-end tube closures. Images were taken 20 days after introduction of fungal cultures. Left and upper row: control plants without TC09 exposure in triplicates per vessel, Left and lower row: plants with exposure to fungal cultures in triplicates per vessel. Arrows indicate TC09 mycelium in closure. Right panel: representative plants of control (left) and TC09-exposed treatment (right). (C) Evaluation of plant growth in greenhouse following in vitro seedling treatment with or without TC09 exposure. Plants with (right three) or without (left two) exposure to TC09 for 20 days were transplanted to soil and maintained in greenhouse using standard management practices. Photograph was taken 70 days after seed sowing.

Next, plant growth stimulation was quantified using a 20-day exposure duration and open-end culture tube closures to prevent excessive condensation in the fungal culture. Exposed plants grew larger and filled up the headspace of culture vessels, produced elongated stems with larger diameters and longer internodes as well as a larger root systems as compared to controls (Figure 1B). Plants were subsequently transplanted to soil and growth differences were maintained (Figure 1C).

As compared to controls, the individual plants following 20-day in vitro exposure treatment exhibited the following growth increase: 25-fold for stem length, 15-fold for shoot biomass, 15-fold for root biomass, 3-fold for base-to-top length of largest leaf, and 10-fold for largest leaf biomass (Figure 2). Results from repeat experiments essentially gave rise to similar increased levels of plant growth with exposure to the fungal culture (data not shown). When planted in soil, the treated plants maintained a near twofold increase in height and in leaf number at 70 days post seed sowing relative to controls (Figures 3A,B).

FIGURE 2
www.frontiersin.org

Figure 2. Quantification of five growth parameters of control and TC09-exposed in vitro tobacco plants. Six-day-old tobacco seedlings following seed sowing were exposed to medium-containing closures (control) or fungal culture containing closures for 20 days and then subject to measurement to determine growth status. Values represent means obtained from triplicate vessels for each treatment. Each vessel contains three individual plants. Data in shaded bars from TC09-exposed plants were converted into fold increase over corresponding values from control plants and labeled in parentheses. Standard errors of the means were marked as vertical lines. Paired treatments sharing a letter on top of bars are not significantly different at P > 0.05 based on Tukey’s test.

FIGURE 3
www.frontiersin.org

Figure 3. Stem growth and leaf production of greenhouse-grown tobacco plants with or without in vitro exposure to TC09. Thirty-day-old plants with or without prior fungal exposure for 20 days were transplanted to soil and maintained in greenhouse using standard management practices. Plant height and the number of visible fully expanded leaves per plant were determined periodically. (A,B) Graphs denote changes in height and leaves, respectively, during the monitored growth period. Values represent average data from nine replicated plants per treatment and two repeated experiments.

Effect of TC09 Exposure Duration on Plant Growth

The potency of PGP activity of TC09 was ascertained using incrementally extended exposure durations. Visual inspection at the end of a 26-day culture period post seed sowing indicated that 1-day exposure of tobacco seedling to the freshly set-up TC09 culture was sufficient to double the plant size as compared to plants without exposure (0 day vs. 1 day) (Figure 4A). Plant growth was incrementally increased after 4-, 10-, and 20-day exposures with 10 days being maximal (Figure 4A). Based on measurements of major growth parameters, 10-day or 20-day exposure duration led to increase in stem length, stem caliper (diameter), and total plant biomass 16-, 3-, and 12-fold, respectively, over the control without TC09 exposure (Figure 4B). Repeated experiments yielded similar results (data not shown).

FIGURE 4
www.frontiersin.org

Figure 4. Effect of MVOC exposure duration on tobacco growth under in vitro conditions. Seedlings 6 days after seed sowing were exposed to TC09 for specified durations from 1, 4, 10 to 20 days as TC09 exposure treatments or maintained without MVOC exposure (0 day as control). Plants were then allowed to grow continuously until the time for the last treatment was met, i.e., 20 days of exposure. (A) Representative plants from each treatment taken at the end of 26-day cultivation. Exposure durations were labeled on each image. (B) Values of seven growth parameters of control and TC09-exposed in vitro tobacco plants determined at the end of 26-day cultivation using various exposure duration. Bar values represent means from triplicate culture vessels for each treatment with each vessel containing three plants. Lines on top denote standard errors. Means sharing a letter in the group label are not significantly different at P > 0.05 according to Tukey’s test.

Effect of Sucrose Concentration and TC09 Exposure on Plant Growth

To investigate whether carbon source availability was a contributing factor for PGP activity, various concentrations of sucrose were tested in conjunction with TC09 exposure for 20 days. Without fungal exposure, tobacco plants did not show any measurable increase in growth rate in response to increasing concentrations of sucrose in the medium (Figure 5). When plants were exposed to TC09, only minimal plant growth promotion was observed in media containing 0 and 5 g/L sucrose whereas significant growth promotion was achieved when plants were maintained on a medium containing > 10 g/L sucrose (Figure 5). These results were also confirmed in a repeat experiment (data not shown).

FIGURE 5
www.frontiersin.org

Figure 5. Effect of sucrose concentration and TC09 exposure on tobacco growth under in vitro conditions. Seedlings 6 days post seed sowing were cultured on MS medium containing various concentrations of sucrose and exposed to freshly prepared TC09 culture in tube closure. (A) Plants were imaged 20 days after inset of fungal exposure. (B) Bars represent average values of growth parameters from three plants in each culture vessel with three replicate vessels per treatment.

Effect of Fungal Culture Media on PGP Activities of TC09

Initial PGP activity of TC09 was characterized using MS medium. Thus, to investigate the influence of culture conditions, several common fungal media were also tested. Variations in visual size and morphology of TC09 mycelium were discernable among various culture media used (Figure 6). Noticeable differences in the level of PGP activity of fungal cultures on various media were also observed (Figure 6). Twenty days post treatment, plants were measured to determine growth differences including total plant height, total plant biomass, stem length, and length of largest leaf. Results revealed that the order of growth stimulation level from highest to lowest among tested fungal culture media was as follows: MS>PDA>Czapek>Yeast>Malt>Hutner’s (Figure 6B). Hutner’s medium contained rich vitamins and the same amount of sucrose as MS medium, but it showed the lowest levels of TC09 growth and PGP activity. On the other hand, Malt and Yeast media, both with reduced PGP activities as compared to MS, encouraged vigorous TC09 growth with comparable colony sizes to that of other media except for Hutner’s medium. In addition, Malt medium seemed to increase hydrophilicity of the mycelium of TC09 and promote hyphal penetration into culture medium (Figure 6A). We conducted a repeat experiment and observed an identical trend of growth stimulation associated with various fungal media (data not shown).

FIGURE 6
www.frontiersin.org

Figure 6. PGP activity of TC09 affected by fungal culture media. (A) Fungal growth or hyphal development in various culture media and corresponding plant growth promotion. TC09 cultures were initiated in closures and used for exposure to 6-day-old tobacco seedlings according to described procedure. Images were taken 2 weeks after exposure initiation. Abbreviated names of fungal culture media are labeled. (B) Major growth parameters of control and TC09-exposed in vitro tobacco plants using various fungal culture media. Measurements were made 20 days after fungal exposure. Values represent means of three plants each of triplicate vessels per treatment. Standard errors of the means were labeled as vertical lines. Means sharing a letter in the group label are not significantly different (P > 0.05) according to Tukey’s test.

Comparison of PGP by TC09 and Other Related Fungi

To investigate whether the observed PGP activity was unique to TC09-or common among related fungal species, we compared a total of seven Cladosporium species/isolates for their ability to promote in vitro tobacco growth. All strains exhibited PGP activity as compared to control without fungal exposure, although there were measurable differences among the species/isolates (Figure 7A). After 20 days of exposure, TC09 gave the highest level or PGP activity in five out of seven growth parameters measured (Table 2). Exposure to C. sphaerospermum NRRL8131 produced the longest stems among all compared fungi (Table 2). Colony morphology and growth pattern of various Cladosporium fungi when cultured on MS medium were also noticeably varied (Figure 7B). Four of the tested fungi including C. sphaerospermum TC09 (1), C. sphaerospermum NRRL8131 (2), C. cladosporioides 113 db (3) and C. asperulatum 208 db (4) produced mycelium in a similarly large diameter and dark greenish brown color. The remaining fungi including C. subtilissimum WF99-209 (5), C. cladosporioides W99-175a (6) and C. macrocarpum Clad ex Phyl 8 (7) formed smaller, black colonies. C. cladosporioides W99-175a (6) is the only fungus which produced dark brownish compounds that penetrated into the culture medium, whereas all other fungi demonstrated hydrophobicity of the mycelium on MS medium (Figure 7B). Subsequently, we conducted a repeat experiment that yielded similar results (data not shown).

FIGURE 7
www.frontiersin.org

Figure 7. Comparison of growth stimulation activity of various isolates/species of Cladosporium. (A) Tobacco plants exposed for 15 days to seven isolates/species of Cladosporium along with controls without exposure (#1). Names of species and isolates of Cladosporium are labeled. F denotes fungal culture in a closure. (B) Mycelium of various compared fungi in closures containing MS medium.

TABLE 2
www.frontiersin.org

Table 2. Fold increase over control of growth parameters from tobacco plants exposed to MVOCs emitted by various Cladosporium fungi.

RNAseq Analysis of Differential Gene Expression (DEG) Associated With TC09 Induced Growth Promotion

To assess host responses at the RNA level, tobacco seedlings treated with and without TC09 exposure for 10 days were subject to RNAseq analysis to identify DEGs. The sampling point was chosen based on the consistent levels of plant growth stimulation achieved following 10 days of MOVC exposure (Figure 4). Experimental design included four replicate TC09 treated plants and four untreated control plants sampled after 10 days of exposure. Approximately 20 million quality reads per sample were obtained. A total of 3,562 DEGs were identified consisting of 1,594 up-regulated and 1,968 down-regulated genes (Supplementary Table S1 and Supplementary Figure S2). Overall, the most highly repressed DEGs included reactive oxygen response genes such as those encoding catalases and superoxide dismutases involved in redox balance as well as photosystem functions including photosystem II D1 core genes along with constituents of the cytochrome and ATP synthase complexes. Among the over-represented DEGs were a number of genes involved in cell wall biosynthesis, cell expansion and multiplication. Numerous genes associated with the biosynthesis and response to phytohormones were differentially expressed with those related to auxin, gibberellin, and ethylene being the most abundant.

JA-inducible genes were notably up-regulated as the result of TC09 exposure. These included genes related to herbivore defense including proteinase inhibitors, Kunitz trypsin inhibitor, and O-methyltransferases – which catalyze multiple reactions in the biosynthesis of furanocoumarins (FCs) that act as insect repellants (Table 3).

TABLE 3
www.frontiersin.org

Table 3. DEGs, associated major biological functions and expression changes from tobacco seedlings with exposure to TC09 for 10 days.

Among cyclin-associated genes, cyclin A and B and two cyclin D genes increased twofold to threefold. Two cyclin-dependent kinases (CDKs) genes increased twofold to eightfold, respectively, in expression. In addition, one CDK regulator gene showed a 2.6-fold up-regulated expression. A number of genes encoding auxin regulators and sugar transporters were also up-regulated. Expression of several gylcosyltransferases functioning in glycosylation was reduced 2- to 10-fold (Table 3).

TC09 Exposure Increases Productivity in Peppers

Two varieties of pepper (Capsicum annuum) were used to investigate whether exposure to TC09 at the seedling stage could positively influence fruit production in a Solanaceous crop plant. Two independent experiments were carried out for each variety. The pepper seedlings were treated with and without TC09 for 20 days under in vitro conditions as described for tobacco. TC09–exposed cayenne and minisweet pepper plants responded to fungal stimulation in a growth pattern similar to tobacco and grew significantly larger than untreated controls that were either started in tissue culture or planted directly to soil. For cayenne pepper, following transplanting to soil, the first flowers among TC09-exposed plants were observed 20 days earlier than control plants along with a denser canopy and more branches (Figure 8A). By 116 days post seed sowing, visual inspection of the root system showed that TC09-exposed plants had more vigorous root growth than both direct soil seeding and tissue culture control plants without fungal exposure (Figure 8B).

FIGURE 8
www.frontiersin.org

Figure 8. Plant growth and fruit production of pepper (Capsicum annuum) varieties cayenne ‘Long Thin’ and sweet bell ‘Minisweet Pepper Mixed’ promoted by exposure in vitro to TC09. TC09 exposure for 20 days at seedling stage was identical to that used for tobacco. (A) Representative cayenne plants from direct seeding, tissue culture and TC09 treatments at 50 days post seed sowing. (B) Representative belowground image of cayenne pepper plants from various treatments at 116 days post seed sowing to depict the density and development of fibrous root mass. (C) Lineup of fruits collected from cayenne pepper (left panel, at 116 days post seed sowing) and minisweet pepper (right panel, at 160 days post seed sowing) from all treatments. Fruits in reddish color indicate vine-ripening.

Cayenne peppers longer than 1 cm in length were collected from all plants at 116 days post seed sowing. Results showed that TC09-exposed plants had the heaviest fruit load and the most vine-ripened fruits (reddish color) among all treatments (Figure 8C, left panel). Similar patterns of fruit production and development were also observed in minisweet pepper when all fruits were collected at 160 days post seed sowing (Figure 8C, right panel). For cayenne pepper, TC09-treated plants produced on average 49 fruit per plant whereas direct seeding and tissue culture-treated plants only put out on average 10 and 18 fruit per plant, respectively. Likewise, for minisweet peppers, the number of fruits per plant from these three treatments were 28, 17, and 17, respectively (Figure 9A). Overall, the number of fruits per plant increased 174% and 62% for these two pepper varieties following exposure to TC09 as compared to tissue culture controls. When fruit weight was determined, cayenne pepper generated 173.1, 34.2, and 55.4 g of collected fruit per plant; and minisweet pepper produced 417.9, 247.8, and 272.0 g per plant for TC09, direct seeding and tissue culture treatments, respectively (Figure 9B). These results indicate that TC09 treatment gave rise to 213% and 54% yield increase over tissue culture control in cayenne and minisweet peppers, respectively. During the period from early 2017 to late 2018, we conducted a total of two experiments with minisweet pepper and three experiments with cayenne pepper. All results were highly consistent (data not shown).

FIGURE 9
www.frontiersin.org

Figure 9. Fruit yield of pepper (Capsicum annuum) varieties cayenne ‘Long Thin’ and sweet bell ‘Minisweet Pepper Mixed’ promoted by exposure in vitro to TC09. Fruits were collected at 116 days and 160 days post seed sowing for cayenne and minisweet peppers, respectively. Greenhouse tests with these two pepper varieties were carried out separately at different time periods of the year due to space limitations. (A) Average number of fruit per plant. (B) Average fresh weight of fruit per plant. Standard errors of the means were labeled as vertical lines on top of data bars. Means sharing a letter in the group label are not significantly different (P > 0.05) according to Tukey’s test.

Discussion

In recent years there have been an increasing number of reports on MVOC-mediated PGP by beneficial and, in some cases, phytopathogenic microorganisms (Ryu et al., 2003; Zou et al., 2010; Minerdi et al., 2011; Paul and Park, 2013; Kai and Piechulla, 2014; Kanchiswamy et al., 2015a; Sánchez-López et al., 2016b; Cordovez et al., 2017; Tahir et al., 2017). Reported MVOCs are able to stimulate photosynthesis, cell expansion and starch accumulation, early flowering and fruit development and enhance host defense and stress tolerance (Ryu et al., 2004; Zhang et al., 2007; Ahemad and Kibret, 2014; Pieterse et al., 2014; Liu and Zhang, 2015; Chung et al., 2016; Sánchez-López et al., 2016a; Cordovez et al., 2017). This work has highlighted potential benefits and opportunities to utilize MVOC-mediated technology to improve agriculture in an environmentally friendly and sustainable fashion (Berg, 2009; Kanchiswamy et al., 2015a; Baez-Rogelio et al., 2016). However, with the relatively low levels of growth enhancement demonstrated thus far and the few plant species studied, the practical application involving direct use of MVOC emitting microorganisms has remained limited (Song and Ryu, 2013; Li et al., 2016; Cordovez et al., 2017; Fincheira and Quiroz, 2018). Furthermore, all effective MVOC species, their interactions and direct correlation between MVOC and plant response need to be determined. In this study, we demonstrated that short term exposure in vitro to an environmentally isolated fungal strain TC09 identified as C. sphaerospermum dramatically promoted tobacco plant growth via MVOCs. We showed in pilot scale experiments under greenhouse conditions that crop productivity of pepper plants could be significantly enhanced by exposure in vitro to TC09 at the seedling stage.

The effects of TC09 exposure on plant growth were largely correlated with the duration of exposure. The stem length of treated tobacco plants doubled after 20 days relative to controls with as little as a 24-h exposure. Exposure duration of 10 days gave maximal PGP as durations beyond 10 days had no significant additive effect. These observed dynamic changes in growth stimulation could potentially be explained by the loss of MVOC emission by TC09 over longer culturing times, saturated plant signaling receptors, or alternatively as a consequence of the depletion of nutrients in the media by rapidly growing plants. To test the latter hypothesis, we performed experiments to test the requirement for sucrose in the tissue culture media and found that sugar concentrations of >5 g/L were necessary for the observed PGP activity but did not enhance PGP beyond 10g/L. These data suggest that endogenous sugar production in young plants is not sufficient to support robust PGP by TC09 and that sustained PGP requires exogenous carbon sources. This finding may also imply that TC09 MVOCs function by activating some signaling pathway(s) in the plant that allows it to better utilize carbon resources available directly through root uptake.

Another contributing factor to PGP by TC09 was the fungal cultivation medium. MS medium supported the highest level of PGP while some common fungal growth media such as Hutner’s medium not only suppressed fungal growth but also reduced PGP activity. MVOC-mediated PGP has been previously shown to vary according to microbial culture conditions. By using rhizobacteria Bacillus mojavensis RRC101, Rath et al. (2018) demonstrated such variability in microbial metabolism in conjunction with PGP activity. When cultured on ½ strength MS medium, the bacteria produced known PGP compounds acetoin and 2,3-butanediol, thus leading to improved growth of Arabidopsis seedlings. However, the use of nutrient agar for bacterial cultivation resulted in the production of relatively high levels of acetone and 2-propanone and correlated with phytotoxicity. In addition, the microbial volatile compound, e.g., β-caryophyllene which is commonly produced by fungi was shown to switch its mode of action from stimulatory to inhibitory depending on concentrations (Wang et al., 2009). Other studies also established that both the diversity and absolute quantity of MVOCs emitted by many microbes are dictated by growth conditions and external stimuli (Sunesson et al., 1995; Weikl et al., 2015; Lee et al., 2016; Nieto-Jacobo et al., 2017). Our findings of altered PGP activities derived from the use of different fungal culture media suggest that culture conditions for TC09 and other PGP microbes can be further exploited and optimized to achieve more effective PGP.

The observed levels of PGP upon exposure of tobacco seedlings to TC09 greatly exceeded those previously reported using other microorganisms (Ryu et al., 2003; Minerdi et al., 2011; Paul and Park, 2013; Bitas et al., 2015; Lee et al., 2016; Sánchez-López et al., 2016b; Cordovez et al., 2017; Tahir et al., 2017). We found that varied levels of PGP activity were also exhibited by diverse species of Cladosporium, however, this activity was largely strain specific. Paul and Park (2013) previously reported an approximately 10-fold increase in fresh weight of tobacco plants exposed to C. cladosporioides CL-1 cultured on PDA for 28 days (4 weeks). The results reported here suggest that differences between various studies could be due to several factors including the specific strain or isolate used, the culture media used for both the plant and fungus, and the availability of sufficient carbon resources to support maximal plant growth rates. Nevertheless, the rapid response to growth stimulants should facilitate further investigation of the mechanisms underlying PGP activity as recently outlined by Fincheira and Quiroz (2018).

A potential limitation for the use of MVOC-mediated PGP is plant damage resulting from the use of phytopathogens or microbes with parasitic behaviors. Phytopathogenic microorganisms can cause adverse effects on target plants in the case of escape and contamination during fungal exposure. In this study, TC09 did not induce any discernable pathogenic responses in all test plants, consistent with its non-pathogenic nature. The non-pathogenic behavior of TC09 may be attributed to its environmental origin and non-adaptive relationship with plants. C. sphaerospermum has long been known as one of the most ubiquitous air-borne, saprobic fungal species and can be found in diverse environments, air and physical structures (Park et al., 2004; Zalar et al., 2007). In contrast, we observed leaf tissue necrosis in tobacco treated with C. sphaerospermum NRRL 8131 (data not shown). This particular isolate was originally referenced as Cladosporium lignicolum Corda based on its sylvan habitat and the ability to degrade and absorb nutrients from lignified woody materials (Dugan et al., 2008). Even though it promoted plant growth similar to TC09, its ability to induce phytotoxic reactions in target plants suggests pathogenic propensity. The ability of TC09 to significantly promote plant growth via MVOCs while lacking harmful phytotoxic effects makes it particularly attractive for further exploitation and the development of practical applications to improve crop productivity.

Transcriptome analyses previously revealed the correlation between differential gene expression and PGP as well as enhanced tolerance to biotic and abiotic stresses following treatment with beneficial microorganisms and MVOCs (e.g., van Loon, 2007; Zhang et al., 2007; Sánchez-López et al., 2016a). Our RNAseq data from untreated and treated tobacco plants provided evidence that diverse metabolic and signaling pathways are altered as a result of exposure to TC09. The most striking observation was that TC09 treatment was accompanied by down-regulation of photosystem functions. This finding is somewhat inconsistent with several previous reports that MVOCs from various microbes caused substantial positive impacts on photosynthesis and chlorophyll production (see review by Fincheira and Quiroz, 2018). The transcriptome data also revealed a potential increase in cell wall metabolism including up-regulation of numerous cellulose synthase genes, endoglucanases, pectinesterases, fasciclins, and xyloglucan endotransglucosylases. A number of putative expansins were also induced which are known to induce slippage of cellulose microfibrils via a non-enzymatic cell wall loosening mechanism and are responsible for cell size increase (Sampedro and Cosgrove, 2005; Choi et al., 2008). In previous MVOC-related studies, Fusarium oxysporum reportedly was able to upregulate expression of an expansin A5 gene in lettuce (Minerdi et al., 2011). Volatiles from rhizobacteria B. subtilis (GB03) also reportedly increased expression of eight expansin genes ∼2-fold (Zhang et al., 2007). Our results indicate that up to 11 expansin genes in treated tobacco plants were up-regulated twofold to fivefold (five genes with more than threefold) over the control.

The rapid growth enhancement following MVOC or microbial exposure may also reflect promoted mitosis and cell cycle that give rise to increased cell numbers. We identified a number of cyclins and CDKs as DEGs in tobacco plants exposed to TC09. Mitotic-specific cyclins and CDKs are known to interact in partner-dependent fashion to form active complexes that function to modulate cell cycle during different phases of cell division (Mironov et al., 1999). This process is also regulated by mitogenic stimulation through protein docking, CDK phosphorylation, and proteolytic degradation of cell cycle proteins (Breyne et al., 2002). Homeostatic alterations in the expression of these proteins has been linked to either faster or slower cell multiplication (Komaki and Sugimoto, 2012). Noticeably, a previous study reported that ectopic over-expression of a cyclin B in Arabidopsis resulted in promoted cell proliferation in roots (Doerner et al., 1996). Our findings provide a direction for further study to address the influence of MVOCs on cell cycle regulation.

Phytohormones including cytokinin, GA3 and auxin can modulate plant growth and development and their activities are dictated by either biosynthesis or homeostasis of active forms. Interestingly, we observed a reduction (2- to 10-fold) in six UDP-glucose glycosyltransferases (UGGTs), which play an important role in the deactivation, homeostasis, storage and secretion of numerous macromolecules including phytohormones via glycosylation modification (Jones and Vogt, 2001; Ostrowski and Jakubowska, 2014). Kim et al. (2015) also reported the down-regulation of a glycosyltransferase in tobacco in response to MVOCs emitted by rhizobacteria B. subtilis although the functional involvement was not elucidated. We postulate that down-regulation of this class of genes may reduce UGGT activity. This consequently leads to minimized deactivation of phytohormones thus potentially increasing the quantity of active phytohormones galvanizing various aspects of plant growth. Early flowering induced by MVOCs was previously attributed to the enhancement of cytokinin action (Sánchez-López et al., 2016b).

Our transcriptome data indicated that the core transcriptional regulator JA-1 in jasmonic acid (JA) signaling pathway was up-regulated more than twofold in tobacco plants exposed to TC09. Also, many herbivore defense genes were up-regulated as well, including proteinase inhibitor II (21-fold), seed storage type protease inhibitor (3.6-fold), Kunitz trypsin inhibitor (2.3-fold) and three cytochrome P450 proteins with sequences homologous to a previously reported protein associated with wound healing and pest resistance (Noordermeer et al., 2001; Dunaevsky et al., 2005). Increased expression levels (threefold to fourfold) of eight latex-like proteins were identified which have been shown to have insecticidal activities against herbivore pests (Konno, 2011). Likewise, seven O-methyltransferases belonging to a class of catalytic enzymes involved in biosynthesis pathway of FCs also showed elevated expression (2- to 15-fold) (Bourgaud et al., 2006, 2014). FCs are secondary metabolites that function mainly as insect repellants (Nitao et al., 2003). These findings prompt future studies to evaluate whether TC09-exposed plants exhibit enhanced resistance to insect pests. Indeed, previous studies have likewise identified MVOC-induced expression of genes associated with JA signaling pathway, broad-spectrum induced systemic resistance (ISR) and herbivore resistance (Song et al., 2013; Naznin et al., 2014; Kanchiswamy et al., 2015a; Chung et al., 2016).

In this study, we demonstrated significant stimulation of plant growth, early reproduction and fruit yield increase in pepper by exposing seedlings to TC09 for a relatively short duration. Although results were achieved under greenhouse conditions, the high level of productivity increase without the additional input of other resources warrants further investigation as to whether such technology could be practically applied at a production scale. Many considerations have to be taken into account including microbe cultivation and conditions, MVOC deployment and dosage control, plant treatment stage and duration, post treatment process and transplanting methodology. In addition, future investigations are needed to study the genome, transcriptome and proteome of TC09 to identify genes, pathways and regulatory mechanisms associated with VOC production and PGP activity. The TC09 strain described here provides a useful tool to carry out such studies and highlights the potential of microbe-based PGP technology to benefit agriculture in an environmentally friendly and sustainable fashion.

Author Contributions

ZTL, WJJ, and CD conceived the concept and designed the experiments. ZTL performed in vitro experiments, analyzed the data, and drafted the manuscript. CD carried out transcriptome assembly and analyses. WJJ, WMJ, and BE conducted fungal identification experiments. ZL and AC provided scientific advice and discussed results. ZTL, CD, WJJ, WMJ, ZL, and AC reviewed the final version of the paper.

Funding

This research was supported in part by grants from AFRS, ARS, USDA and the Oak Ridge Institute for Science and Education (ORISE).

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank Dr. Frank M. Dugan for kindly providing Cladosporium fungi and also gratefully acknowledged Dr. Margaret Pooler, Research Leader, FNPR, ARS-USDA, for providing financial support. We also thank Dr. Tracy C. Leskey, Director, AFRS, ARS-USDA, for excellent scientific advice and support for this research and Mark A. Demuth, Elizabeth Lutton, and Dennis R. Bennett for their excellent technical assistance. The handling and movement of all fungi described in the current study were carried using Standard Operation Procedures in accordance with the Animal and Plant Health Inspection Service of the United States Department of Agriculture. Acquisition and movement of mentioned fungal materials were authorized under a permit (P526P-17-02230) issued by the agency.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2018.01959/full#supplementary-material

FIGURE S1 | Neighbor-joining dendrogram of ITS sequences associated with TC09. The 249 bp ITS3/4 amplicon consensus sequence derived from PCR amplification using DNA samples of the fungal isolate was used to search GenBank database for homologous sequences. More than 148 sequence entries were retrieved and subject to phylogenetic analysis. Neighbor-joining method was used to reconstruct the phylogenetic tree with bootstrap values indicated for each branch. Sequence entries belonging to same species were labeled using identical colors. Asterisk indicates the caudal position of the fungal isolate within the monophyletic group Cladosporium sphaerospermum.

FIGURE S2 | Heat map depicting high (red) and low (blue) relative levels of differentially expressed genes (DEGs) in individual plants with and without exposure to TC09. Six-day-old tobacco seedlings were maintained under in vitro conditions with or without a 10-day exposure to TC09 and then subject to total RNA extraction using shoot tip tissues that include apical meristem and three to four young leaves. Heat map was generated by using CLC Genomics Workbench software program following transcriptome assembly using tomato transcriptome as a reference. Plant treatment type is marked by red (control) and blue (TC09-treated) line on top. There were four replicated individual plants for each treatment. Color-rendered scale of expression changes is marked on bottom.

FIGURE S3 | Colony of Cladosporium sphaerospermum TC09 on PDA medium (A); and confocal microscopy micrographs of branching chains of conidia (B); intercalary and terminal conidia (C); and mycelium (D).

TABLE S1 | List of all tobacco DEGs after exposure in vitro to TC09 for 10 days. Fold change in expression levels was calculated based on means of four biological replicates for both control and MVOC-treated plants. Genes were annotated using GO terms and tomato (Solanum lycopersicum) reference genes available on https://solgenomics.net.

References

Ababutain, I. M. (2013). Effect of some ecological factors on the growth of Aspergillus niger and Cladosprium sphaerospermum. Am. J. Appl. Sci. 10, 159–163. doi: 10.3844/ajassp.2013.159.163

CrossRef Full Text | Google Scholar

Ahemad, M., and Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria, current perspective. J. King Saud. Univ. Sci. 26, 1–20. doi: 10.1016/j.jksus.2013.05.001

CrossRef Full Text | Google Scholar

Ahmad, F., Ahmad, I., and Khan, M. S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microb. Res. 163, 173–181. doi: 10.1016/j.micres.2006.04.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Audrain, B., Létoffé, S., and Ghigo, J. M. (2015). Airborne bacterial interactions: functions out of thin air? Front. Microbiol. 6:1476. doi: 10.3389/fmicb.2015.01476

CrossRef Full Text | Google Scholar

Baez-Rogelio, A., Morales-Garcıa, Y. E., Quintero-Hernandez, V., and Munoz-Rojas, J. (2016). Next generation of microbial inoculants for agriculture and bioremediation. Microb. Biotechnol. 10, 19–21. doi: 10.1111/1751-7915.12448

PubMed Abstract | CrossRef Full Text | Google Scholar

Barea, J. M., Pozo, M. J., Azcon, R., and Azcon-Aguilar, C. (2005). Microbial co-operation in the rhizosphere. J. Exp. Bot. 56, 1761–1778. doi: 10.1093/jxb/eri197

PubMed Abstract | CrossRef Full Text | Google Scholar

Berg, G. (2009). Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl. Microbiol. Biotechnol. 84, 11–18. doi: 10.1007/s00253-009-2092-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Berg, G., and Smalla, K. (2009). Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiol. Ecol. 68, 1–13. doi: 10.1111/j.1574-6941.2009.00654.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Bitas, V., McCartney, N., Li, N., Demers, J., Kim, J. E., Kim, H. S., et al. (2015). Fusarium Oxysporum volatiles enhance plant growth via affecting auxin transport and signaling. Front. Microb. 6:1248. doi: 10.3389/fmicb.2015.01248

PubMed Abstract | CrossRef Full Text | Google Scholar

Bourgaud, F., Hehn, A., Larbat, R., Doerper, S., Gontier, E., Kellner, S., et al. (2006). Biosynthesis of coumarins in plants: a major pathway still to be unravelled for cytochrome P450 enzyme. Phtochem 5, 293–308. doi: 10.1007/s11101-006-9040-2

CrossRef Full Text | Google Scholar

Bourgaud, F., Olry, A., and Hehn, A. (2014). “Recent advances in molecular genetics of furanocoumarin synthesis in higher plants,” in Recent Advances in Redox Active Plant and Microbial Products, eds C. Jacob, G. Kirsch, A. Slusarenko, P. Winyard, and T. Burkholz (Dordrecht: Spinger).

Google Scholar

Breyne, P., Dreesen, R., Vandepoele, K., De Veylder, L., Van Breusegem, F., Callewaert, L., et al. (2002). Transcriptome analysis during cell division in plants. PNAS 99, 14825–14830. doi: 10.1073/pnas.222561199

PubMed Abstract | CrossRef Full Text | Google Scholar

Choi, D., Kim, J. H., and Lee, Y. (2008). Expansins in plant development. Adv. Bot. Res. 47, 47–97. doi: 10.1016/S0065-2296(08)00002-5

CrossRef Full Text | Google Scholar

Chung, J. H., Song, G. C., and Ryu, C. M. (2016). Sweet scents from good bacteria: case studies on bacterial volatile compunds for plant growth and immunity. Plant Mol. Biol. 90, 677–687. doi: 10.1007/s11103-015-0344-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Cordovez, V., Mommer, L., Moisan, K., Lucas-Barbosa, D., Pierik, R., Mumm, R., et al. (2017). Plant phenotypic and transcriptional changes induced by volatiles from the fungal root pathogen Rhizoctonia solani. Front. Plant Sci. 8:1262. doi: 10.3389/fpls.2017.01262

PubMed Abstract | CrossRef Full Text | Google Scholar

De Souza, R., Ambrosini, A., and Passaglia, L. M. P. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genet. Mol. Biol. 38, 401–419. doi: 10.1590/S1415-475738420150053

PubMed Abstract | CrossRef Full Text | Google Scholar

De-la-Peña, C., and Loyola-Vargas, V. M. (2014). Biotic interactions in the rhizosphere: a diverse cooperative enterprise for plant productivity. Plant Physiol. 166, 701–719. doi: 10.1104/pp.114.241810

PubMed Abstract | CrossRef Full Text | Google Scholar

Doerner, P., Jørgensen, J. E., You, R., Steppuhn, J., and Lamb, C. (1996). Control of root growth and development by cyclin expression. Nature 380, 520–523. doi: 10.1038/380520a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Dugan, F. M., Braun, U., Groenwald, J. Z., and Crous, P. W. (2008). Morphological plasticity in Cladosporium sphaerospermum. Persoonia 21, 9–16. doi: 10.3767/003158508X334389

PubMed Abstract | CrossRef Full Text | Google Scholar

Dunaevsky, Y. E., Elpidina, E. N., Vinokurov, K. S., and Belozersky, M. A. (2005). Protease inhibitors in improvement of plant resistance to pathogens and insects. Plant Mol. 39, 702–708. doi: 10.1007/s11008-005-0076-y

CrossRef Full Text | Google Scholar

Farag, M. A., Song, G. C., Park, Y. S., Audrain, B., Lee, S., Ghigo, J. M., et al. (2017). Biological and chemical strategies for exploring inter- and intra-kingdom communication mediated via bacterial volatile signals. Nat. Protoc. 12:1359. doi: 10.1038/nprot.2017.023

PubMed Abstract | CrossRef Full Text | Google Scholar

Fincheira, P., and Quiroz, A. (2018). Microbial volatiles as plant growth inducers. Microbiol. Res. 208, 63–75. doi: 10.1016/j.micres.2018.01.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Jones, P., and Vogt, T. (2001). Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers. Planta 213, 164–174. doi: 10.1007/s004250000492

PubMed Abstract | CrossRef Full Text | Google Scholar

Kai, M., and Piechulla, B. (2014). Impact of volatiles of the rhizobacteria Serratia odorifera on the moss Physcomitrella patens. Plant Signal. Behav. 5, 444–446. doi: 10.4161/psb.5.4.11340

PubMed Abstract | CrossRef Full Text | Google Scholar

Kanchiswamy, C. N., Malnoy, M., and Maffei, M. E. (2015a). Bioprospecting bacterial and fungal volatiles for sustainable agriculture. Trends Plant Sci. 20, 206–211. doi: 10.1016/j.tplants.2015.01.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Kanchiswamy, C. N., Malnoy, M., and Maffei, M. E. (2015b). Chemical diversity of microbial volatiles and their potential for plant growth and productivity. Front. Plant Sci. 6:151. doi: 10.3389/fpls.2015.00151

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, J. S., Lee, J., Seo, S. G., Lee, C., Woo, S. Y., and Kim, S. H. (2015). Gene expression profile affected by volatiles of new plant growth promoting rhizobacteria, Bacillus subtilis strain JS, in tobacco. Genes Genom. 37, 387–397. doi: 10.1007/s13258-015-0267-4

CrossRef Full Text | Google Scholar

Komaki, S., and Sugimoto, K. (2012). Control of the plant cell cycle by developmental and environmental cues. Plant Cell Physiol. 53, 953–964. doi: 10.1093/pcp/pcs070

PubMed Abstract | CrossRef Full Text | Google Scholar

Konno, K. (2011). Plant latex and other exudates as plant defense systems: roles of various defense chemicals and proteins contained therein. Phytochem 72, 1510–1530. doi: 10.1016/j.phytochem.2011.02.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Kremer, R. J., and Souissi, T. (2001). Cyanide production by rhizobacteria and potential for suppression of weed seedling growth. Curr. Microb. 43, 182–186. doi: 10.1007/s002840010284

PubMed Abstract | CrossRef Full Text | Google Scholar

Lambers, H., Mougel, C., Jaillard, B., and Hinsinger, P. (2009). Plant-microbe-soil interactions in the rhizosphere: an evolutionary perspective. Plant Soil 321, 83–115. doi: 10.1007/s11104-009-0042-x

CrossRef Full Text | Google Scholar

Lee, S., Yap, M., Behringer, G., Hung, R., and Bennett, J. W. (2016). Volatile organic compounds emitted by Trichoderma species mediate plant growth. Fungal Biol. Biotechnol. 3:7. doi: 10.1186/s40694-016-0025-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Lemfack, M. C., Nickel, J., Dunkel, M., Preissner, R., and Piechulla, B. (2014). mVOC, a database of microbial volatiles. Nucleic Acids Res. 42, D744–D748. doi: 10.1093/nar/gkt1250

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, N., Alfiky, A., Vaughan, M. M., and Kang, S. (2016). Stop and smell the fungi: fungal volatile metabolites are overlooked signals involved in fungal interaction with plants. Fungal Biol. Rev. 30, 134–144. doi: 10.1016/j.fbr.2016.06.004

CrossRef Full Text | Google Scholar

Liu, X. M., and Zhang, H. (2015). The effects of bacterial volatile emission on plant abiotic stress tolerance. Front. Plant Sci. 6:774. doi: 10.3389/fpls.2015.00774

PubMed Abstract | CrossRef Full Text | Google Scholar

Minerdi, D., Bossi, S., Maffei, M. E., Gullino, M. L., and Garibaldi, A. (2011). Fusarium oxysporum and its bacterial consortium promote lettuce growth and expansin A5 gene expression through microbial volatile organic compound (MVOC) emission. FEMS Microbiol. Ecol. 76, 342–351. doi: 10.1111/j.1574-6941.2011.01051.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Mironov, V., De Veylder, L., Van Maontagu, M., and Inze, D. (1999). Cyclin-dependent kinases and cell division in plants-the nexus. Plant Cell 11, 509–521.

PubMed Abstract | Google Scholar

Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15, 473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x

CrossRef Full Text | Google Scholar

Nadeem, S. M., Ahmad, M., Zahir, Z. A., Javaid, A., and Ashraf, M. (2014). The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotech. Adv. 32, 429–448. doi: 10.1016/j.biotechadv.2013.12.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Naznin, H. A., Kiyohara, D., Kimura, M., Miyazawa, M., Shimizu, M., and Hyakumachi, M. (2014). Systemic resistance induced by volatile organic compounds emitted by plant growth-promoting fungi in Arabidopsis thaliana. PLoS One 9:e86882. doi: 10.1371/journal.pone.0086882

PubMed Abstract | CrossRef Full Text | Google Scholar

Nieto-Jacobo, M. F., Steyaert, J. M., Salazar-Badillo, F. B., Nguyen, D. V., Rostás, M., Braithwaite, M., et al. (2017). Environmental growth conditions of Trichoderma spp. Affects indole acetic acid derivatives, volatile organic compounds, and plant growth promotion. Front. Plant Sci. 8:102. doi: 10.3389/fpls.2017.00102

PubMed Abstract | CrossRef Full Text | Google Scholar

Nitao, J. K., Berhow, M., Duval, S. M., Weisleder, D., Vaughn, S. F., Zangerl, A., et al. (2003). Characterization of furanocoumarin metabolites in parsnip webworm, Depressaria pastinacella. J. Chem. Ecol. 29, 671–682. doi: 10.1023/A:1022872704016

PubMed Abstract | CrossRef Full Text | Google Scholar

Noordermeer, M. A., Veldink, G. A., and Vliegenthart, J. F. G. (2001). fatty acid hydroperoxide lyase: a plant cytochrome P450 enzyme involved in would healing and pest resistance. Chembiochem 2, 494–504. doi: 10.1002/1439-7633(20010803)2:7/8<494::AID-CBIC494>3.0.CO;2-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Ostrowski, M., and Jakubowska, A. (2014). UDP-glycosyltransferases of plant hormones. Adv. Cell Biol. 4, 43–60. doi: 10.2478/acb-2014-0003

CrossRef Full Text | Google Scholar

Park, H. G., Managbanag, J. R., Stamenova, E. K., and Jong, S. C. (2004). Comparative analysis of common indoor Cladosporium species based on molecular data and conidial characters. Mycotaxon 89, 441–451.

Google Scholar

Paul, D., and Park, K. S. (2013). Identification of volatile produced by Cladosporium cladosporioides CL-1, a fungal biocontrol agent that promotes plant growth. Sensors 13, 13969–13977. doi: 10.3390/s131013969

PubMed Abstract | CrossRef Full Text | Google Scholar

Peñuelas, J., Asensio, D., Tholl, D., Wenke, K., Rosenkranz, M., Piechulla, B., et al. (2014). Biogenic volatile emissions from the soil. Plant Cell Environ. 37, 1866–1891. doi: 10.1111/pce.12340

PubMed Abstract | CrossRef Full Text | Google Scholar

Pieterse, C. M. J., Zamioudis, C., Berendsen, R. L., Weller, D. M., VanWees, S. C. M., and Bakker, P. A. H. M. (2014). Induced systemic resistance by beneficial microorganisms. Annu. Rev. Phytopathol. 52, 347–375. doi: 10.1146/annurev-phyto-082712-102340

PubMed Abstract | CrossRef Full Text | Google Scholar

Rath, M., Mitchell, T. R., and Gold, S. E. (2018). Volatiles produced by Bacillus mojavensis RRC101 act as plant growth modulators and are strongly culture-dependent. Microbiol. Res. 208, 76–84. doi: 10.1016/j.micres.2017.12.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Ryu, C. M., Farag, M. A., Hu, C. H., Reddy, M. S., Kloepper, J. W., and Pare, P. W. (2004). Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol. 134, 1017–1026. doi: 10.1104/pp.103.026583

PubMed Abstract | CrossRef Full Text | Google Scholar

Ryu, C. M., Farag, M. A., Hu, C. H., Reddy, M. S., Wei, H. X., Pare, P. W., et al. (2003). Bacterial volatiles promote growth in Arabidopsis. PNAS 100, 4927–4932. doi: 10.1073/pnas.0730845100

PubMed Abstract | CrossRef Full Text | Google Scholar

Saharan, B. S., and Nehra, V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sci. Med. Res. 21, 1–30.

Google Scholar

Sampedro, J., and Cosgrove, D. J. (2005). The expansin superfamily. Genome Biol. 6:242. doi: 10.1186/gb-2005-6-12-242

PubMed Abstract | CrossRef Full Text | Google Scholar

Sánchez-López, A. M., Bahaji, A., De Diego, N., Baslam, M., Li, J., Muñoz, F. J., et al. (2016a). Plant response to fungal volatiles is triggering by mechanisms independent of plastid phosphoglucose isomerase. Plant Physiol. 172, 1989–2001. doi: 10.1104/pp.16.00945

PubMed Abstract | CrossRef Full Text | Google Scholar

Sánchez-López, A. M., Baslam, M., De Diego, N., Muñoz, F. J., Bahaji, A., Almagro, G., et al. (2016b). Volatile compounds emitted by diverse phytopathogenic microorganisms promote plant growth and flowering through cytokinin action. Plant Cell Environ. 39, 2592–2608. doi: 10.1111/pce.12759

PubMed Abstract | CrossRef Full Text | Google Scholar

Schulz, S., and Dickschat, J. S. (2007). Bacterial volatiles: the smell of small organisms. Nat. Prod. Rep. 24, 814–842. doi: 10.1039/b507392h

PubMed Abstract | CrossRef Full Text | Google Scholar

Sinclair, J. B., and Dhingra, O. D. (1995). Basic Plant Pathology Methods. Boca Raton, FL: CRC Press Inc.

Google Scholar

Song, G. C., and Ryu, C. M. (2013). Two volatile organic compounds trigger plant self-defense against a bacterial pathogen and a sucking insect in cucumber under open field conditions. Int. J. Mol. Sci. 14, 9803–9819. doi: 10.3390/ijms14059803

PubMed Abstract | CrossRef Full Text | Google Scholar

Song, G. C., Ryu, S. Y., Kim, Y. S., Lee, J. Y., Choi, J. S., and Ryu, C. M. (2013). Elicitation of induced resistance against Pectobacterium carotovorum and Pseudomonas syringae by specific individual compounds derived from native Korean plant species. Molecules 18, 12877–12895. doi: 10.3390/molecules181012877

PubMed Abstract | CrossRef Full Text | Google Scholar

Sunesson, A. L., Vaes, W. H. J., Nilsson, C. A., Blomquist, G., Andersson, B., and Carlson, R. (1995). Identification of volatile metabolites from five fungal species cultivated on two media. Appl. Environ. Microb. 61, 2911–2918.

PubMed Abstract | Google Scholar

Tahir, H. A. S., Gu, Q., Wu, H., Raza, W., Hanif, A., Wu, L., et al. (2017). Plant growth promotion by volatile organic compounds produced by Bacillus subtilis SYST2. Front. Microbiol. 8:171. doi: 10.3389/fmicb.2017.00171

PubMed Abstract | CrossRef Full Text | Google Scholar

Thakore, Y. (2006). The biopesticide market for global agricultural use. Ind. Biotechnol. 2, 194–208. doi: 10.1089/ind.2006.2.194

CrossRef Full Text | Google Scholar

The Tomato Genome Consortium (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature 485, 635–641. doi: 10.1038/nature11119

PubMed Abstract | CrossRef Full Text | Google Scholar

Turner, S., and Meadows-Smith, H. (2016). Selection for rare biostimulant microorganisms - finding the proverbial needles in the rhizosphere haystack. Acta Hort. 1148, 105–108. doi: 10.17660/ActaHortic.2016.1148.13

CrossRef Full Text

van Loon, L. C. (2007). Plant responses to plant growth-promoting rhizobacteria. Eur. J. Plant Pathol. 119, 243–254. doi: 10.1007/s10658-007-9165-1

CrossRef Full Text | Google Scholar

Wang, R. L., Peng, S. L., Zeng, R. S., Ding, L. W., and Xu, Z. F. (2009). Cloning, expression and wounding induction of beta-caryophyllene synthase gene from Mikania micrantha HBK and allelopathic potential of beta-caryophyllene. Allelopathy J. 24, 35–44.

Google Scholar

Wardle, D. A., Bardgett, R. D., Klironomos, J. N., Setala, H., Van der Putten, W. H., and Wall, D. H. (2004). Ecological linkages between aboveground and belowground biota. Science 304, 1629–1633. doi: 10.1126/science.1094875

PubMed Abstract | CrossRef Full Text | Google Scholar

Weikl, F., Ghirardo, A., Schnitzler, J. P., and Pritsch, K. (2015). Sesquiterpene emissions from Alternaria alternate and Fusarium oxysporum: effects of age, nutrient availability, and co-cultivation. Nature 6:22152.

Google Scholar

Weise, T., Kai, M., and Piechulla, B. (2013). Bacterial ammonia causes significant plant growth inhibition. PLoS One 8:e63538. doi: 10.1371/journal.pone.0063538

PubMed Abstract | CrossRef Full Text | Google Scholar

Whipps, J. (2001). Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52, 487–511. doi: 10.1093/jxb/52.suppl_1.487

CrossRef Full Text | Google Scholar

White, T. J., Bruns, T., Lee, S., and Taylor, J. W. (1990). “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics,” in PCR Protocols: A Guide to Methods and Applications, 1st Edn, eds M. A. Innis, D. H. Gelfandm, J. J. Sninsky, and T. J. White (New York, NY: Academic Press),315–322.

PubMed Abstract | Google Scholar

Zalar, P., de, Hoog GS, Schroers, H. J., Crous, P. W., Groenewald, J. Z., and Gunde-Cimerman, N. (2007). Phylogeny and ecology of the ubiquitous saprobe Cladosporium sphaerospermum, with descriptions of seven new species from hypersaline environments. Stud. Mycol. 58, 157–183. doi: 10.3114/sim.2007.58.06

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, H., Kim, M. S., Krishnamachari, V., Payton, P., Sun, Y., Grimson, M., et al. (2007). Rhizobacterial volatile emissions regulate auxin homeostasis and cell expansion in Arabidopsis. Planta 226, 839–851. doi: 10.1007/s00425-007-0530-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Zou, C., Li, Z., and Yu, D. (2010). Bacillus megaterium strain XTBG34 promotes plant growth by producing 2-pentylfuran. J. Microbiol. 48, 460–466. doi: 10.1007/s12275-010-0068-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: microbial volatile organic compounds, Cladosporium sphaerospermum, plant growth promotion, biostimulant, increased productivity, Nicotiana tabacum L., Capsicum annuum L., expression profiling

Citation: Li ZT, Janisiewicz WJ, Liu Z, Callahan AM, Evans BE, Jurick WM II and Dardick C (2019) Exposure in vitro to an Environmentally Isolated Strain TC09 of Cladosporium sphaerospermum Triggers Plant Growth Promotion, Early Flowering, and Fruit Yield Increase. Front. Plant Sci. 9:1959. doi: 10.3389/fpls.2018.01959

Received: 26 September 2018; Accepted: 17 December 2018;
Published: 01 February 2019.

Edited by:

Giuseppe Colla, Università degli Studi della Tuscia, Italy

Reviewed by:

Philipp Franken, Leibniz-Institut für Gemüse- und Zierpflanzenbau (IGZ), Germany
Paolo Bonini, Ngalab, Spain
Antonio Ferrante, University of Milan, Italy

Copyright © 2019 Li, Janisiewicz, Liu, Callahan, Evans, Jurick and Dardick. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Chris Dardick, chris.dardick@ars.usda.gov