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Original Research ARTICLE

Front. Plant Sci., 26 May 2020 | https://doi.org/10.3389/fpls.2020.00683

Functional Relationships of Wood Anatomical Traits in Norway Spruce

  • 1Department of Geography, Faculty of Earth Sciences and Geography, University of Cambridge, Cambridge, United Kingdom
  • 2Swiss Federal Research Institute for Forest, Snow and Landscape Research (WSL), Birmensdorf, Switzerland
  • 3Department of Chemistry, Life Science and Sustainability, University of Parma, Parma, Italy
  • 4Institute of Biosciences and Bioresources, Italian National Research Council, Florence, Italy
  • 5Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Ancona, Italy
  • 6Global Change Research Institute, Czech Academy of Sciences, Brno, Czechia
  • 7Department of Geography, Faculty of Science, Masaryk University, Brno, Czechia

The quantitative assessment of wood anatomical traits offers important insights into those factors that shape tree growth. While it is known that conduit diameter, cell wall thickness, and wood density vary substantially between and within species, the interconnection between wood anatomical traits, tree-ring width, tree height and age, as well as environment effects on wood anatomy remain unclear. Here, we measure and derived 65 wood anatomical traits in cross-sections of the five outermost tree rings (2008–2012) of 30 Norway spruce [Picea abies (L.) H. Karst.] trees growing along an altitudinal gradient (1,400–1,750 m a.s.l.) in the northern Apennines (Italy). We assess the relationship among each anatomical trait and between anatomical trait groups according to their function for (i) tree-ring growth, (ii) cell growth, (iii) hydraulic traits, and (iv) mechanical traits. The results show that tree height significantly affects wood hydraulic traits, as well as number and tangential diameter of tracheids, and ultimately the total ring width. Moreover, the amount of earlywood and latewood percentage influence wood hydraulic safety and efficiency, as well as mechanical traits. Mechanically relevant wood anatomical traits are mainly influenced by tree age, not necessarily correlated with tree height. An additional level of complexity is also indicated by some anatomical traits, such as latewood lumen diameter and the cell wall reinforcement index, showing large inter-annual variation as a proxy of phenotypic plasticity. This study unravels the complex interconnection of tree-ring tracheid structure and identifies anatomical traits showing a large inter-individual variation and a strong interannual coherency. Knowing and quantifying anatomical variation in cells of plant stem is crucial in ecological and biological studies for an appropriate interpretation of abiotic drivers of wood formation often related to tree height and/or tree age.

Introduction

Every single cell in a tree ring is derived from the division of a cambial cell (periclinal growth), which then expands to its final size, thickens its cell wall and eventually lignifies before programmed cell death (Plomion et al., 2001). These steps are triggered and guided by the complex interplay of genetic factors, ontogenesis (organism development over time), hormone regulation, and environmental conditions (Aloni, 2013). Wood cell formation, therefore, represents a unique and complex combination of interacting processes resulting in a complete tree ring (Vaganov et al., 2006; Fonti and Jansen, 2012). An extensive literature faced the task of relating the wood cellular structure to its functioning (e.g., Tyree and Ewers, 1991; Domec et al., 2009; Fonti et al., 2013; Hacke et al., 2015; Beeckman, 2016; De Micco et al., 2019). For example, tracheids diameter and density, earlywood–latewood percentage, and cell wall thickness (CWT) have been demonstrated to play a central role in regulating tree hydraulic efficiency and mechanical functioning (e.g., McCulloh et al., 2010; Rosner, 2013; Prendin et al., 2018), but also to be strongly influenced by climate (e.g., Larson, 1994; Carrer et al., 2016; Castagneri et al., 2017). Differently, some hydraulic parameters, such as hydraulic diameter and specific hydraulic conductivity, are influenced by tree height (e.g., Anfodillo et al., 2013; Rosell et al., 2017). However, inter- and intra-individual variation of multiple wood anatomical traits have been rarely analyzed on large numbers of trees growing under different environmental conditions (Rungwattana and Hietz, 2017; Prendin et al., 2018). As a consequence, it is still unclear how relevant is the contribution of each factor balancing the trade-off at the wood cell, and consequently, at the tree-ring level. Dendrophenotypic traits (e.g., Evans et al., 2018; Heer et al., 2018) and the development of optimal strategies for quantifying their variation are important for assessing the effect of potential drivers of individual variation in growth traits. Recently, several studies brought a new perspective on the inter-individual variation of wood anatomical traits highlighting the role of tree height as the driver of wood anatomical variation along plant stem, as opposed to the classical pith-to-bark age trend (Enquist, 2002; Olson et al., 2014; Kašpar et al., 2019). This is especially true for conduits in woody species, designed to transport water from roots to leaves. According to fluid dynamics, the increase in plant height would be accompanied with a concomitant increase in hydrodynamic resistance along the conduit (West et al., 1997). This effect could constrain height growth of a plant, unless it is compensated by a dimensional increase of wood conduits during ontogenesis. It has been repeatedly observed that conduit diameter becomes larger (and longer) toward the plant base as the distance to the tree-top increases, a pattern called “conduit widening” (Anfodillo et al., 2013; Lazzarin et al., 2016; Rosell et al., 2017). The conduit widening, when viewed on a wood cross-section from the stem base, is represented by an increase in conduit size from pith-to-bark (as described by Malpighi, 1675) and it is often interpreted as an age-related trend (Gartner, 1995; Lachenbruch et al., 2011). In a short plant bearing narrow conduits, the increase in conduit size from pith-to-bark is a consequence of the conduits getting larger to compensate for a height increase (Carrer et al., 2015) (Figure 1).

FIGURE 1
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Figure 1. Sketch of the wood structure scaling from pith-to-bark in relation to tree height (modified from Rosell et al., 2017) in a hypothetical 5-years-old tree, supposing the same radial (tree-ring width) and longitudinal (internode length) growth. The change in number and size of tracheids is shown from pith to bark. There are more and smaller tracheids in short trees (or close to the pith), and fewer and wider tracheids in tall trees (or close to the bark) compared to the previously formed ring.

To investigate the synergy of tree height, age, and environmental growing conditions on wood anatomical traits we quantified wood anatomical variation in cross-sections of Norway spruce, a conifer species featuring a simple wood structure where tracheids have both conductive and mechanical functions (Evert, 2006). The aims of this paper are to investigate (1) how tracheid anatomy in radial and tangential directions is related to ontogenesis, (2) how wood anatomical variation reflects functional constraints and trade-offs, and (3) how wood anatomical traits vary at the inter-individual and inter-annual level. To minimize the climate influence on wood anatomical traits, considered “noise” in this study, only the five outermost tree rings (2008–2012) were analyzed. The results provided a summary of wood anatomical trait variability and suggested possible implications for related research fields such as plant ecology and evolutionary genetics.

Materials and Methods

Study Site and Samples Collection

Field sampling was carried out in 2012 in the Campolino Natural Reserve (northern Apennines) (Table 1), which is the southernmost known autochthonous population of Norway spruce [Picea abies (L.) H. Karst.] in Italy (Chiarugi, 1936; Magri et al., 2015; Avanzi et al., 2019). Three plots were established along an altitudinal transect on the same slope. The high altitude plot (CAM-H, ∼1,730 m a.s.l.) is a pure, dense and relatively young spruce forest recolonizing an old pasture. The intermediate plot (CAM-E, ∼1,615 m a.s.l.) is a mature mixed forest with Norway spruce, silver fir (Abies alba Mill.) and European beech (Fagus sylvatica L.). The lowest plot (CAM-L, ∼1,475 m a.s.l.) is a similar mixed forest, but with less spruce (Table 1). In each plot sampled, trees were labeled and georeferenced with a geographic positioning system device (GPS; Garmin, 2 m resolution). Stem diameter at breast height (DBH) and tree height were measured, and one increment core was collected with a 5 mm Pressler borer at DBH in cross-slope direction to avoid compression wood.

TABLE 1
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Table 1. Geographic coordinates and stand characteristics of the three Norway spruce [Picea abies (L.) H. Karst.] plots.

The three plots fall in the same spatial grid, so monthly mean temperatures and total precipitation for the 2008–2012 period were obtained from the CRU TS V.4.0 (land) 0.5° grid (KNMI Climate Explorer1).

Tree Selection and Wood Anatomical Traits

A total of 10 trees per plot (Table 1, Supplementary Figure S1) were selected to maximize variation in tree height, age and DBH (Supplementary Figure S2). Wood microsections were prepared to collect high-resolution anatomical images following the standard protocols (Gärtner and Schweingruber, 2013; von Arx et al., 2016; Prendin et al., 2017). From each core, 12–15 μm thick cross-section from the last five tree rings (dated 2008–2012) were cut using a WSL lab-microtome (Gärtner et al., 2015a, b). All sections were double-stained using Safranin and Astra blue, dehydrated with ethanol, and permanently fixed on a microscope slide with Canada balsam. Overlapping images of cross-sections were captured at 100 times magnification using a digital camera (Canon EOS 650D, Canon Inc., Tokyo, Japan) through an Olympus BX41 microscope (Olympus Corp., Tokyo, Japan). PTGUI v10 (New House Internet Services B.V., Rotterdam, NL, United States) was used to stitch single images and produce a high-resolution composite image (2.36 pixels/μm). These images were then processed with the software ROXAS (v3.0.88, von Arx and Carrer, 2014), which provides a large set of measurements of tree-ring width (TRW) and wood anatomical traits (Table 2). Image analysis and anatomical data collection was restricted to a width of one tangential millimeter within each tree ring.

TABLE 2
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Table 2. List of all wood anatomical traits used in this study, dividing by their function.

The wood anatomical traits used in this study were direct measurements and derived from ROXAS output (Table 2). The analysis was performed within a tree ring, and in the earlywood (EW) and latewood (LW). TRW, EW and LW width (EWW and LWW), and their percentage (EW% and LW%) calculated as the ratio of EWW (LWW) to TRW, as well as their ratio (LW/EW) were measured. To tell apart EW and LW tracheids, ROXAS automatically computes the Mork’s index which is the ratio between twice the double CWT and the lumen diameter, both measured in radial direction (Denne, 1988) (Table 2). Additionally, the ratio between radial and tangential diameter of tracheid lumen was applied to discriminate between EW (ratio > 1) and LW (ratio < 1) tracheid shape. The number of tracheids in tangential direction was manually counted in the LW, and tracheid number in radial direction was computed by dividing the total number of tracheids in each tree ring (ROXAS output) by the number of tracheids in tangential direction within 1 mm strip. Wood anatomical traits were grouped in traits related to (i) tree-ring growth, (ii) cell growth, (iii) hydraulic efficiency and hydraulic safety, and (iv) mechanical support and carbon investment (Table 2).

Statistical Analysis

Univariate and Multivariate Analysis of Wood Anatomical Traits

In order to assess the variability of wood anatomical traits, their attributed functions and the ontogenetic effect, univariate and multivariate analyses were performed. For assessing the pairwise relationships among wood anatomical traits, and the effect of tree height and tree age, linear, exponential and logarithmic functions were fitted, and the function with the highest R2 was chosen. The R2 of the logarithmic and exponential regressions were calculated by log-transforming the x- and y-axes, respectively. A principal component analysis (PCA) was carried out on each single year dataset using the prcomp function in R (R Core Team, 2018) to simultaneously describe the relationships among the 65 anatomical traits scored.

Identifying the Main Sources of Wood Anatomical Variation

The effects of tree height, age and plot (the latter used as a proxy for environmental variation along the altitudinal transect) on principal component (PC) scores summarizing wood anatomical variation, were assessed using a multiple regression approach through the lm function in R. The first three PC scores calculated on the 2012 dataset (when tree height data was available) were used as dependent variables in the multiple regression. For each PC, the Anova function in the R package car (Fox and Weisberg, 2011) was used to perform a backward model selection. A variable was dropped when the Likelihood Ratio Test on nested models indicated a simpler model structure as more parsimonious. Additionally, Mantel tests were performed between the matrix of pairwise geographic distances and the matrix of individual differences in PC scores to test whether spatial proximity determines more similar wood anatomical traits. Such analysis was performed at the plot level using the R package vegan. The effect of climatic variation was minimized by confining all analyses to the same five calendar years (2008–2012).

Assessing Inter-Annual Stability of Wood Anatomical Variation

A PCA was performed on each tree-ring dataset of wood anatomical traits relative to each year of the selected time period. To quantify the correlation of individual wood anatomical traits among years, a Procrustes test was performed on PC scores of different years using the protest function of the R package vegan (Oksanen et al., 2018). This statistical test measures the correlation between two point configurations (i.e., PC scores). Pairwise correlations between all possible combinations of years were visualized using the procrustes function, showing the amplitude and the direction of PC scores changes. Additionally, a coefficient of variation (CV) among years was calculated for each wood anatomical trait at the individual level. Individual CVs were then averaged among individuals to rank wood anatomical traits in terms of their stability through time.

Results

Inter-Individual Variation of Wood Anatomical Traits and Their Relationships: Univariate Analysis

Tree-ring width was positively correlated with the number of tracheids per radial row (r = 0.99). TRW is EW width dependent (R2 = 0.993, P < 0.001), showing on average an EW% higher than 80% (Figure 2A). TRW was also positively related to the ratio between radial and tangential diameter (Drad/Dtan) of tracheid lumen (R2 = 0.435, P < 0.001) (Figure 2B). The lumen size maintained a proportional increase in radial and tangential diameter only in EW but not in LW tracheids (Supplementary Figure S3).

FIGURE 2
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Figure 2. (A) Tree-ring width (TRW) of the five outermost tree rings as a function of earlywood percentage (EW%). (B) TRW as a function of the ratio between radial diameter (Drad) and tangential diameter (Dtan) of the tracheid lumen. Values of Drad/Dtan ratio >1 indicate that the shape of the tracheid is more EW-like. The inset figure represents a graphical sketch of earlywood and latewood tracheids as seen in a cross-section, describing the radial and tangential diameter, and their ratio. The dashed line represents the Drad/Dtan ratio equal to one. The three symbols (circle, diamond, and triangle) correspond to trees from CAM-H (red), CAM-E (blue), and CAM-L (green) plots. Both relationships were best described by an exponential function (***P < 0.001).

Because tree height measurements prior to the year of sampling were not available, only Drad and Dtan of the outermost ring (2012) can be related to tree height. Dtan was more related to tree height than Drad, and these relationships were strongest for EW (EW Dtan: R2 = 0.536, EW Drad: R2 = 0.390, and LW Dtan: R2 = 0.357, P < 0.001) (Figure 3A). The Drad/Dtan scaled with tree height: taller trees had proportionally larger Dtan in EW (R2 = 0.530, P < 0.001), meaning that the tangential diameter increased more with tree height than Drad (Figure 3B). As a consequence, the tangential number of tracheids was negatively related to tree height (R2 = 0.473, P < 0.001).

FIGURE 3
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Figure 3. (A) Radial diameter (Drad) of EW tracheid lumen in bright color, and tangential diameter (Dtan) of EW tracheid lumen in pale color as a function of tree height. (B) Negative relationship between the ratio of radial and tangential diameter of tracheid lumen (Drad/Dtan) and tree height in bright color for EW portion and in pale color for the LW portion. The data shown are for the last year (2012). Each symbol (circle, diamond, and triangle) corresponds to a tree from the three plots CAM-H (red), CAM-E (blue), and CAM-L (green). All relationships are significant (*P < 0.05, **P < 0.01, ***P < 0.001).

Tracheid diameter affected both cell density (CD), the number of tracheids per mm2, and their lumen area (LA). Both CD and LA were related to tree height but with opposite directions: CD decreases with tree height, while LA increases (Supplementary Figure S4).

Both mean hydraulic diameter (Dh) and specific hydraulic conductivity (Ks) were positively related to tree height (Supplementary Figure S5) while, theoretical hydraulic conductivity (Kh) was not. Although Kh is positively related to Drad (R2 = 0.503), total number of tracheids (CNO) (R2 = 0.556), EW% (R2 = 0.615), basal area increment (BAI) (R2 = 0.672), TRW (R2 = 0.707), and EW width (R2 = 0.722) (all relationships are significant, P < 0.001) (Figures 4A,B). In addition high EW% corresponded to larger values of “hydraulic carbon use efficiency” index (HCUE) (R2 = 0.600, P < 0.001) (Supplementary Figure S6). HCUE increased linearly with TRW (R2 = 0.772, P < 0.001) and decreased exponentially with age (R2 = 0.358, P < 0.001), meaning that younger trees had a high carbon investment per unit of water conductivity.

FIGURE 4
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Figure 4. (A) Total conductivity (Kh) as a function of earlywood width (EWW). The inset figures express the same relationship for each plot: CAM-H (top), CAM-E (middle), and CAM-L (bottom). A linear function was fitted, except in CAM-H where a logarithmic function shows the highest R2. (B) Total conductivity (Kh) as a function of earlywood percentage (EW%). The inset figures express the same relationship for each plot: CAM-H (top), CAM-E (middle), and CAM-L (bottom). An exponential function was fitted. Each symbol (circle, diamond, and triangle) corresponds to a tree from the three plots CAM-H (red), CAM-E (blue), and CAM-L (green). All relations are significant (***P < 0.001).

Earlywood percentage (LW%) had a negative (positive) effect on cell wall reinforcement index measured in EW tracheids ([t/b_05]2) (Figure 5A). Trees with denser wood (due to larger LW%, R2 = 0.638, P < 0.001 – Supplementary Figure S7) showed higher values of [t/b]2 in the EW portion (Figure 5B). The relative wood density (RWD) was also negatively related to Drad and Dtan (R2 = 0.618 and R2 = 0.316, respectively, P < 0.001) (Supplementary Figure S7), as well as positively related to CWT (R2 = 0.526, P < 0.001).

FIGURE 5
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Figure 5. (A) Cell wall reinforcement index ([t/b_05]2) in EW portion (5th percentile) as a function of earlywood percentage (EW%). A logarithmic function was fitted. (B) Cell wall reinforcement index ([t/b]2) in EW portion (5th percentile) as a function of relative wood density in the earlywood. Each symbol (circle, diamond, and triangle) corresponds to a tree from the three plots CAM-H (red), CAM-E (blue), and CAM-L (green). An exponential function was fitted. All relationships are significant (***P < 0.001).

Cell wall thickness in radial cell walls was positively correlated with the CWT in tangential cell walls (Figure 6). In the LW portion of the ring, this relationship showed a higher intercept, as opposed to the 1:1 relationship in the EW, with the increase in tangential direction stronger than in the radial direction. A weak but positive relation between CWT and tree age was also found (R2 = 0.281, P < 0.001), whereas no relation was observed between tree height and CWT.

FIGURE 6
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Figure 6. Cell wall thickness in radial direction (CWT rad) as a function of cell wall thickness in tangential (CWT tan) direction. The inset figures represent the same relationship for EW and LW portion. Each symbol (circle, diamond, and triangle) corresponds to trees from the three plots CAM-H (red), CAM-E (blue), and CAM-L (green). The linear regressions are significant (***P < 0.001). The dashed red line represents the 1:1 relationship.

Inter-Individual Variation of Wood Anatomical Traits and Their Relationships: Multivariate Analysis

A PCA on wood anatomical traits relative to the 2012 tree ring showed that the first three PCs explains the 36.8, 28.5, and 13.3% of variation, respectively (Figure 7). The hydraulic traits Kh and HCUE were correlated positively with PC1, while [t/b]2 and [t/b_05]2 negatively (Figure 7, Supplementary Figure S8). The remaining hydraulic traits (Dh and Ks) were positively correlated with PC2, meaning that they were orthogonal with respect to the other hydraulic traits. All mechanical and carbon-allocation traits (Table 2, Supplementary Figure S8) showed a negative correlation with PC1, whereas PC2 was highly correlated with some wood anatomical traits connected to tangential diameter in both EW and LW, and the LA of the EW portion (Figure 7, Supplementary Figure S8).

FIGURE 7
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Figure 7. Results of the PCA on all anatomical traits measured for the 2012 tree ring. Arrows represent the eigenvalue of each variable for the first two principal components (PC). Arrow colors indicate the function assigned to each anatomical trait according to the literature (see Table 2). Arrows names are written in Supplementary Figure S8. The three symbols (circle, diamond, and triangle) correspond to individual scores on PC1 (x-axis) and PC2 (y-axis) from CAM-H, CAM-E, and CAM-L plots, respectively.

The results of the multiple regression analysis between the first three PCs and tree height, age and plot were reported in Supplementary Table S1. Scores of PC1 increased with tree height (ß = 0.470, P = 0.031) and decreased with age (ß = −0.082, P = 0.006; Supplementary Figure S9A). Scores of PC2 increased with tree height (ß = 0.546, P < 0.001; Supplementary Figure S9B). Scores of PC3 increased with tree height (ß = 0.218, P = 0.037) and were significantly determined by the plot (P = 0.006; Supplementary Figure S9C). Scores of PC3 were significantly different between CAM-H and CAM-L (adj P = 0.023). Pairwise geographic distances and pairwise differences calculated on PC scores were positively correlated only in CAM-H (r = 0.357, P = 0.019) (Supplementary Figure S10). This means that, in CAM-H, trees that are spatially closer to each other have similar wood anatomical traits.

Inter-Annual Variation of Wood Anatomical Traits

Principal component analysis was performed on the dataset relative to each tree ring (from 2008 to 2012) to test the stability of wood anatomical traits through time. Procrustes tests show consistently that individual PC scores were highly correlated from year to year (r ≥ 0.8, P = 0.001; Figure 8A). Only 22 out of 65 anatomical traits displayed a mean CV larger than 10% and a range of individual CVs larger than 20% (Figure 8B). The largest inter-annual and inter-individual variation was displayed by [t/b]2 and LA of the 1st and 5th percentiles (LW portion) (Table 2, Figure 8B).

FIGURE 8
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Figure 8. (A) Results from the Procrustes test analyzing the stability of PC scores through time. Correlation coefficients and their statistical significance are shown in panels above the diagonal. Blue lines represent shifts in the position of individual trees among years. (B) Mean and range of individual coefficient of variation (CV) among years for each anatomical trait. Traits characterized by large variation among years were labeled.

Discussion

Within a Tree Ring: Ontogenetic Trend and Tracheid Anatomy

Besides confirming that taller trees have larger tracheids in the basal portion of their stem, our results take the discussion forward. EW tracheids in tall trees become larger by increasing both in radial and tangential directions proportionally. However, the tangential diameter of tracheids is partly constrained by the diameter of cambial mother cells and by adjacent tracheids. Despite previous works confirming that tracheid size increases primarily in the radial direction, whereas the tangential diameter and length remain almost constant (Skene, 1972; Cuny et al., 2014), here we suggest that the radial tracheid diameter, and even more their tangential diameter, are primarily influenced by tree height and to a lesser extent by the altitudinal position along the transect. This confirms recent results on conifers growing at the treeline: once the LA of EW tracheids is standardized for tree height, this trait is not related to temperatures (Kašpar et al., 2019).

Wood Anatomical Variation: Functional Constraints and Trade-Offs

All 65 wood anatomical traits in this study are grouped according to their wood characteristics and function in tree-ring growth, cell growth, hydraulic efficiency and safety, and mechanical support and carbon investment. PCA appears as an efficient analysis for summarizing the information embedded within a large set of wood anatomical traits and shows a high degree of variation both between and within plots (Supplementary Figure S11). We identify two main wood anatomical “strategies” associated with the first and second PCs, respectively (Figure 6). On the one hand, high PC1 scores describe trees with high EW percentages and hydraulic conductivity, whereas low PC1 scores describe trees with high LW% and high RWD, thicker tracheid walls, and high cell wall reinforcement index, traits linked to hydraulic safety. On the other hand, PC2 describes whether each tree invests in tracheid number or in their lumen diameter. High PC2 scores identify trees with few large tracheids, while low PC2 scores identify trees with numerous small ones. Since PCs are orthogonal, this second anatomical strategy is independent to the first one. PC1 is negatively related to tree age showing that older trees tend to have a higher proportion of LW, thicker cell wall in both radial and tangential direction, and higher LW RWD than younger trees, which may limit their water transport capacity, increase the investment in carbon and increase their resistance to embolism (Domec and Gartner, 2002; Rosner et al., 2014) as explained in the following section. PC2 is strongly, positively related to tree height, meaning that taller trees tend to have fewer but larger tracheids to maximize water conductivity per unit area. Tall trees have a high hydraulic tracheid diameter and Ks at breast height (Rosner et al., 2008; Anfodillo et al., 2012; Olson et al., 2014), whereas the Kh is mainly related to the amount of EW: the higher the EW percentage, the higher is the conductivity.

Recent studies have shown a plant size-dependent sensitivity to drought (Bennett et al., 2015; Fajardo et al., 2018; Stovall et al., 2019). Bennett et al. (2015) found that drought has more detrimental impacts on the stem diameter growth of large trees. Stovall et al. (2019) reported that tree height is the most important predictor of tree death during drought episodes, with tall trees (>30 m) dying at a rate of more than double than short trees (<15 m). However, tall or large trees are not always old trees, and the lack of such correlation depends on species characteristics (e.g., shade-tolerant vs light-tolerant species) and within-population structure, such as local site conditions, tree’s disturbance history, growth form, as well as individual genotype (Lindenmayer and Laurance, 2016). Finally, the site conditions can play an important role in wood anatomical trait variability. A significant plot-effect is found on PC3; in particular, a statistically significant difference between PC3 scores of CAM-H and CAM-L. This difference may suggest an altitudinal effect as these two plots are the most contrasting in terms of elevation (1,730 vs 1,475 m). However, PC3 explained just 13.3% of the total inter-individual wood anatomical variation, suggesting that the environment is, in any case, less relevant than tree height and age in affecting wood anatomical trait variation. This could be partially related to the limited extension of the altitudinal gradient explored (∼300 m) that, however, encompasses the elevation gradient of spruce in the study area. For this reason, it could be worth exploring the inter-individual variation of wood anatomical traits along larger and replicated altitudinal transects and in more limiting environmental conditions. A weak but statistically significant spatial effect is found in CAM-H, where neighbor trees display more similar anatomical traits. In this case, spatial proximity is considered as a proxy for micro-environmental heterogeneity. Avanzi et al. (2019) demonstrated the relevance of micro-environmental heterogeneity of growth patterns on a larger dendrochronological dataset of trees sampled in the same plots. In this study, we analyzed 10 trees per plot, not a high number for fully capturing the micro-environmental heterogeneity.

Wood Anatomical Traits at the Inter-Individual and Inter-Annual Level

Our analysis on inter-annual variation of wood anatomical traits investigates which traits are likely to show no or small changes through time. PCA results are highly correlated among years, meaning that overall trees have similar wood anatomical traits in their last formed five rings. This result emphasizes, over a limited temporal window, how trees exhibit globally coherent wood anatomical patterns, even with different mean temperatures and total precipitation of the growing season (Supplementary Figure S12). Nonetheless, the coefficients of variation for each single wood anatomical trait reveal that the magnitude of such inter-annual variation changes among traits and individuals. We found that the cell wall reinforcement index of the entire ring, the LA of LW, and LW% are the most variable traits both at inter- and intra-individual level. LW is probably the wood anatomical trait mostly involved in potentially adaptive responses to climate and environmental changes. According to Larson (1969), EW to LW ratio is influenced by tree crown size that, in turn, depends on stand density and competition. When growing conditions are ideal, height growth is enhanced and the LW% is lowered (Domec and Gartner, 2002). In our study site, the EW percentage is on average >80%, pointing toward a weak effect of climate on individual tree growth dynamics, as shown by a climate-growth correlation analysis performed on a larger dataset of trees sampled from the same plots (Avanzi et al., 2019).

Wood Density and Hydraulic Vulnerability

Latewood percentage affects RWD and also the hydraulic properties of coniferous wood (Hacke et al., 2001; Domec and Gartner, 2002). In Norway spruce, EW tracheids are the most effective water-conducting pipes with higher numbers of pits per conduit length unit compared to LW tracheids (Rosner, 2013). Wood density shows moderate to high values of heritability in conifers (e.g., Hannrup et al., 2004; Martinez Meier et al., 2008; Chen et al., 2018) and, despite that this trait is not directly linked to hydraulic vulnerability, it is involved in mechanisms of drought tolerance and hydraulic failure, with higher wood density being associated with lower vulnerability to cavitation (e.g., Dalla-Salda et al., 2009; Rosner, 2013; Rosner et al., 2016; Fernández et al., 2019). As in previous studies (Bouche et al., 2014; Bouriaud et al., 2015), the present results show that RWD in LW is more related to tree age than to tree height, and that trees with higher RWD in LW feature a higher cell wall reinforcement index. We used the cell wall reinforcement index as a proxy for hydraulic vulnerability (e.g., Hacke et al., 2001; Rosner, 2013), concluding that trees with high RWD are more resistant to tracheid embolism. Studies on LW, EW, and transition-wood tracheids suggest that, when a water-deficit episode takes place, cavitation first occurs and expands in the LW tracheids. Only when water deficit further increases, embolism spreads in the EW and finally reaches the transition wood (Dalla-Salda et al., 2014). However, the loss in conductivity is not related to where the initial cavitation occurs, but to the wood anatomical structure (e.g., RWD and EW to LW ratio) that shapes the species vulnerability to drought events (Luss et al., 2019).

Implications for Ecological and Biological Studies

In conclusion, our findings seem to consistently demonstrate that it is crucial in any wood anatomical, ecological, and evolutionary genetics study to record and consider tree height to correctly investigate inter-individual wood anatomical variation. By doing so, wood anatomical traits can be standardized based on tree height as opposed to tree diameter which is not always directly related to tree age. Additionally, we also consider crucial to account for age-related trends that might affect wood anatomical traits with mechanical support functions, such as CWT, RWD, and lumen diameter of LW tracheids. Such traits can ultimately affect tree resistance to embolism. We recommend that analyzing wood anatomical traits within few tree rings but from a large number of individuals is a good method for capturing most of the variance in both wood anatomical traits and microenvironmental conditions. This strategy may avoid the “noise” created by difference in tree height, and age. Finally, the creation of a common garden would be extremely useful to determine whether and how individual genotypes drive wood anatomical variation, minimizing microsite heterogeneity.

Data Availability Statement

The datasets generated for this study are available on request to the corresponding author.

Author Contributions

All authors planned and designed the research. AlP, AnP, CA, and CU conducted the fieldwork. AlP collected the data. AlP, GA, AnP, CA, and AC analyzed the data. AlP led manuscript writing with contributions from all authors. All authors contributed critically to the drafts and gave final approval for publication.

Funding

AlP has been supported by Marche Polytechnic University (Italy) for her abroad post-doc (year 2016) at the Swiss Federal Research Institute (WSL) which provided technical and scientific support. PF and GA have been supported by the Swiss contribution from the State Secretariat for Education, Research and Innovation (SERI) to the EU H2020 project GenTree (Nr. 676876). UB received funding from the project “SustES – Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions” (CZ.02.1.01/0.0/0.0/16_019/0000797). AnP and CA have been supported by the ERAnet BiodivERsA project “TipTree” (ANR-12-EBID-0003).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We would like to thank Paul J. Krusic for his comments, Ufficio per la Biodiversità of Pistoia and Italian State Forest Service of Abetone (PT) for logistic support to field activities. Thanks to Valeria Gallucci, Matteo Garbarino, Cristina Leonarduzzi, and Daniele Castagneri for helping during fieldwork and to Sandro Morganti, Maria Celeste Labriola, Catia Boggi, and Ilaria Spanu for technical assistance during lab work.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00683/full#supplementary-material

Footnotes

  1. ^ https://climexp.knmi.nl

References

Aloni, R. (2013). “The role of hormones in controlling vascular differentiation,” in Cellular Aspects of Wood Formation, ed. J. Fromm, (Berlin: Springer), 99–139. doi: 10.1007/s00425-013-1927-8

CrossRef Full Text | Google Scholar

Anfodillo, T., Deslauriers, A., Menardi, R., Tedoldi, L., Petit, G., and Rossi, S. (2012). Widening of xylem conduits in a conifer tree depends on the longer time of cell expansion downwards along the stem. J. Exp. Bot. 63, 837–845. doi: 10.1093/jxb/err309

CrossRef Full Text | Google Scholar

Anfodillo, T., Petit, G., and Crivellaro, A. (2013). Axial conduit widening in woody species: a still neglected anatomical pattern. IAWA J. 34, 352–364.

Google Scholar

Avanzi, C., Piermattei, A., Piotti, A., Büntgen, U., Heer, K., Opgenoorth, L., et al. (2019). Disentangling the effects of spatial proximity and genetic similarity on individual growth performances in Norway spruce natural populations. Sci. Total Environ. 650, 493–504. doi: 10.1016/j.scitotenv.2018.08.348

CrossRef Full Text | Google Scholar

Beeckman, H. (2016). Wood anatomy and trait-based ecology. IAWA J. 37, 127–151.

Google Scholar

Bennett, A. C., McDowell, N. G., Allen, C. D., and Anderson-Teixeira, K. J. (2015). Larger trees suffer most during drought in forests worldwide. Nat. Plants 1:15139. doi: 10.1038/nplants.2015.139

CrossRef Full Text | Google Scholar

Bouche, P. S., Larter, M., Domec, J. C., Burlett, R., Gasson, P., Jansen, S., et al. (2014). A broad survey of hydraulic and mechanical safety in the xylem of conifers. J. Exp. Bot. 65, 4419–4431. doi: 10.1093/jxb/eru218

CrossRef Full Text | Google Scholar

Bouriaud, O., Teodosiu, M., Kirdyanov, A. V., and Wirth, C. (2015). Influence of wood density in tree-ring-based annual productivity assessments and its errors in Norway spruce. Biogeosciences 12, 6205–6217.

Google Scholar

Carrer, M., Brunetti, M., and Castagneri, D. (2016). The imprint of extreme climate events in century- long time series of wood anatomical traits in high-elevation conifers. Front. Plant Sci. 7:683. doi: 10.3389/fpls.2016.00683

CrossRef Full Text | Google Scholar

Carrer, M., von Arx, G., Castagneri, D., and Petit, G. (2015). Distilling allometric and environmental information from time series of conduit size: the standardization issue and its relationship to tree hydraulic architecture. Tree Physiol. 35, 27–33. doi: 10.1093/treephys/tpu108

CrossRef Full Text | Google Scholar

Castagneri, D., Fonti, P., von Arx, G., and Carrer, M. (2017). How does climate influence xylem morphogenesis over the growing season? Insights from long-term intra-ring anatomy in Picea abies. Ann. Bot. 119, 1011–1020. doi: 10.1093/aob/mcw274

CrossRef Full Text | Google Scholar

Chen, Z. Q., Baison, J., Pan, J., Karlsson, B., Andersson, B., Westin, J., et al. (2018). Accuracy of genomic selection for growth and wood quality traits in two control-pollinated progeny trials using exome capture as the genotyping platform in Norway spruce. BMC Genomics 19:946. doi: 10.1186/s12864-018-5256-y

CrossRef Full Text | Google Scholar

Chiarugi, A. (1936). L’indigenato della “Picea excelsa” nell’Appennino Etrusco. Nuovo G. Bot. Ital. 63, 131–166.

Google Scholar

Cuny, H. E., Rathgeber, C. B., Frank, D., Fonti, P., and Fournier, M. (2014). Kinetics of tracheid development explain conifer tree-ring structure. New Phytol. 203, 1231–1241. doi: 10.1111/nph.12871

CrossRef Full Text | Google Scholar

Dalla-Salda, G., Fernández, M. E., Sergent, A. S., Rozenberg, P., Badel, E., and Martinez Meier, A. (2014). Dynamics of cavitation in a Douglas-fir tree-ring: transition-wood, the lord of the ring? J. Plant Hydr. 1:e0005.

Google Scholar

Dalla-Salda, G., Martinez-Meier, A., Cochard, H., and Rozenberg, P. (2009). Variation of wood density and hydraulic properties of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) clones related to a heat and drought wave in France. For. Ecol. Manag. 257, 182–189.

Google Scholar

De Micco, V., Carrer, M., Rathgeber, C. B. K., Camarero, J. J., Voltas, J., Cherubini, P., et al. (2019). From xylogenesis to tree rings: wood traits to investigate tree response to environmental changes. IAWA J. 40, 155–182.

Google Scholar

Denne, M. P. (1988). Definition of latewood according to Mork (1928). IAWA Bull. 10, 59–62.

Google Scholar

Domec, J. C., and Gartner, B. L. (2002). How do water transport and water storage differ in coniferous earlywood and latewood? J. Exp. Bot. 53, 2369–2379. doi: 10.1093/jxb/erf100

CrossRef Full Text | Google Scholar

Domec, J. C., Warren, J. M., Meinzer, F. C., and Lachenbruch, B. (2009). Safety for xylem failure by implosion and air-seeding within roots, trunks and branches of young and old conifer trees. IAWA J. 30, 101–120.

Google Scholar

Enquist, B. J. (2002). Universal scaling in tree and vascular plant allometry: toward a general quantitative theory linking plant form and function from cells to ecosystems. Tree Physiol. 22, 1045–1064. doi: 10.1093/treephys/22.15-16.1045

CrossRef Full Text | Google Scholar

Evans, M. E. K., Gugger, P. F., Lynch, A. M., Guiterman, C. H., Fowler, J. C., Klesse, S., et al. (2018). Dendroecology meets genomics in the common garden: new insights into climate adaptation. New Phytol. 218, 401–403. doi: 10.1111/nph.15094

CrossRef Full Text | Google Scholar

Evert, R. F. (2006). Esau’s Plant Anatomy, Meristems, Cells, and Tissues of the Plant Body – Their Structure, Function, and Development. Hoboken: Wiley.

Google Scholar

Fajardo, A., Mcintire, E. J. B., and Olson, M. E. (2018). When short stature is an asset in trees. Trends Ecol. Evol. 34, 193–199. doi: 10.1016/j.tree.2018.10.011

CrossRef Full Text | Google Scholar

Fernández, M. E., Barotto, A. J., Meier, A. M., Gyenge, J. E., Tesón, N., Quiñones Martorello, A. S., et al. (2019). New insights into wood anatomy and function relationships: how eucalyptus challenges what we already know. For. Ecol. Manag. 454:117638.

Google Scholar

Fonti, P., Bryukhanova, M. V., Myglan, V. S., Kirdyanov, A. V., Naumova, O. V., and Vaganov, E. A. (2013). Temperature-induced responses of xylem structure of Larix sibirica (Pinaceae) from the Russian Altay. Am. J. Bot. 100, 1332–1343. doi: 10.3732/ajb.1200484

CrossRef Full Text | Google Scholar

Fonti, P., and Jansen, S. (2012). Xylem plasticity in response to climate. New Phytol. 195, 734–736. doi: 10.1111/j.1469-8137.2012.04252.x

CrossRef Full Text | Google Scholar

Fox, J., and Weisberg, S. (2011). An R Companion to Applied Regression, Second Edition. Thousand Oaks, CA: Wiley.

Google Scholar

Gartner, B. L. (1995). “Patterns of xylem variations within a tree and their hydraulic and mechanical consequences,” in Plant Stems: Physiology and Functional Morphology, ed. B. L. Gartner, (San Diego: Academic Press), 125–149.

Google Scholar

Gärtner, H., Cherubini, P., Fonti, P., von Arx, G., Schneider, L., Nievergelt, D., et al. (2015a). A technical perspective in modern Tree-ring Research - how to overcome dendroecological and wood anatomical challenges. J. Vis. Exp. 97:e52337. doi: 10.3791/52337

CrossRef Full Text | Google Scholar

Gärtner, H., Lucchinetti, S., and Schweingruber, F. H. (2015b). A new sledge microtome to combine wood anatomy and tree-ring ecology. IAWA J. 36, 452–459.

Google Scholar

Gärtner, H., and Schweingruber, F. H. (2013). Microscopic Preparation Techniques for Plant Stem Analysis. Birmensdorf: Verlag-WSL.

Google Scholar

Hacke, U. G., Lachenbruch, B., Pitterman, J., Mayr, S., Domec, J. C., and Schulte, P. J. (2015). “The hydraulic architecture of conifers,” in Functional and Ecological Xylem Anatomy, ed. U. G. Hacke, (Switzerland: Springer International Publishing), 39–75.

Google Scholar

Hacke, U. G., Sperry, J. S., Pockman, W. T., Davis, S. D., and McCulloh, K. A. (2001). Trends in wood density and structure are linked to prevention of xylem implosion by negative pressure. Oecologia 126, 457–461. doi: 10.1007/s004420100628

CrossRef Full Text | Google Scholar

Hannrup, B., Cahalan, C., Chantre, G., Grabner, M., Karlsson, B., Le Bayon, I., et al. (2004). Genetic parameters of growth and wood quality traits in Picea abies. Scand. J. For. Res. 19, 14–29.

Google Scholar

Heer, K., Behringer, D., Piermattei, A., Bässler, C., Brandl, R., Fady, B., et al. (2018). Linking dendroecology and association genetics in natural popu- lations: stress responses archived in tree rings associate with SNP genotypes in silver fir (Abies alba Mill.). Mol. Ecol. 27, 1428–1438. doi: 10.1111/mec.14538

CrossRef Full Text | Google Scholar

Jyske, T., and Hölttä, T. (2015). Comparison of phloem and xylem hydraulic architecture in Picea abies stems. New Phytol. 205, 102–115. doi: 10.1111/nph.12973

CrossRef Full Text | Google Scholar

Kašpar, J., Anfodillo, T., and Treml, V. (2019). Tree size mostly drives the variation of xylem traits at the treeline ecotone. Trees 33, 1657–1665.

Google Scholar

Lachenbruch, B., Moore, J. R., and Evans, R. (2011). “Radial variation in wood structure and function in woody plants, and hypotheses for its occurrence,” in Size- and Age-Related Changes in Tree Structure and Function, eds F. C. Meinzer, B. Lachenbruch, and T. E. Dawson, (Dordrecht: Springer), 121–164.

Google Scholar

Larson, P. R. (1969). Wood Formation and the Concept of Wood Quality. New Haven, CT: Yale University.

Google Scholar

Larson, P. R. (1994). The Vascular Cambium, Development and Structure. Berlin: Springer-Verlag.

Google Scholar

Lazzarin, M., Crivellaro, A., Williams, C. B., Dawson, T. E., Mozzi, G., and Anfodillo, T. (2016). Tracheid and pit anatomy vary in tandem in a tall Sequoiadendron giganteum tree. IAWA J. 37, 172–185.

Google Scholar

Lindenmayer, D. B., and Laurance, W. F. (2016). The unique challenges of conserving large old trees. Trends Ecol. Evol. 31, 416–418. doi: 10.1016/j.tree.2016.03.003

CrossRef Full Text | Google Scholar

Luss, S., Lundqvist, S., Evans, R., Grahn, T., Olsson, L., Petit, G., et al. (2019). Within-ring variability of wood structure and its relationship to drought. IAWA J. 40, 288–310. doi: 10.1163/22941932-40190216

CrossRef Full Text | Google Scholar

Magri, D., Agrillo, E., Di Rita, F., Furlanetto, G., Pini, R., Ravazzi, C., et al. (2015). Holocene dynamics of tree taxa populations in Italy. Rev. Palaeobot. Palynol. 218, 267–284.

Google Scholar

Malpighi, M. (1675). Anatome Plantarum. London: Martyn.

Google Scholar

Martinez Meier, A. G., Sanchez, L., Salda, G., Pastorino, M. J. M., Gautry, J.-Y., Gallo, L. A., et al. (2008). Genetic control of the tree-ring response of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) to the 2003 drought and heat-wave in France. Ann. For. Sci. 65, 102–102.

Google Scholar

McCulloh, K., Sperry, J. S., Lachenbruch, B., Meinzer, F. C., Reich, P. B., and Voelker, S. (2010). Moving water well: comparing hydraulic efficiency in twigs and trunks of coniferous, ring-porous, and diffuse porous saplings from temperate and tropical forests. New Phytol. 186, 439–450. doi: 10.1111/j.1469-8137.2010.03181.x

CrossRef Full Text | Google Scholar

Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., et al. (2018). Vegan: Community Ecology Package. R Package Version 2.4–6.

Google Scholar

Olson, M. E., Anfodillo, T., Rosell, J. A., Petit, G., Crivellaro, A., Isnard, S., et al. (2014). Universal hydraulics of the flowering plants: vessel diameter scales with stem length across angiosperm lineages, habits and climates. Ecol. Lett. 17, 988–997. doi: 10.1111/ele.12302

CrossRef Full Text | Google Scholar

Plomion, C., Leprovost, G., and Stokes, A. (2001). Wood formation in trees. Plant Physiol. 127, 1513–1523.

Google Scholar

Prendin, A. L., Petit, G., Carrer, M., Fonti, P., Björklund, J., and von Arx, G. (2017). New research perspectives from a novel approach to quantify tracheid wall thickness. Tree Physiol. 37, 976–983. doi: 10.1093/treephys/tpx037

CrossRef Full Text | Google Scholar

Prendin, A. L., Petit, G., Fonti, P., Rixen, C., Dawes, M. A., and von Arx, G. (2018). Axial xylem architecture of Larix decidua exposed to CO2 enrichment and soil warming at the tree line. Funct. Ecol. 32, 273–287.

Google Scholar

R Core Team (2018). R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing.

Google Scholar

Rosell, J. A., Olson, M. E., and Anfodillo, T. (2017). Scaling of xylem vessel diameter with plant size: causes, predictions, and outstanding questions. Curr. Forest. Rep. 3, 46–59.

Google Scholar

Rosner, S. (2013). Hydraulic and biomechanical optimization in Norway spruce trunk wood: a review. IAWA J. 34, 365–390.

Google Scholar

Rosner, S., Klein, A., Müller, U., and Karlsson, B. (2008). Tradeoffs between hydraulic and mechanical stress responses of mature Norway spruce trunk wood. Tree Physiol. 28, 1179–1188. doi: 10.1093/treephys/28.8.1179

CrossRef Full Text | Google Scholar

Rosner, S., Svetlik, J., Andreassen, K., Borja, I., Dalsgaard, L., Evans, R., et al. (2014). Wood density as a screening trait for drought sensitivity in Norway spruce. Can. J. For. Res. 44, 154–161.

Google Scholar

Rosner, S., Světlík, J., Andreassen, K., Børja, I., Dalsgaard, L., Evans, R., et al. (2016). Novel hydraulic vulnerability proxies for a boreal conifer species reveal that opportunists may have lower survival prospects under extreme climatic events. Front. Plant Sci. 7:831. doi: 10.3389/fpls.2016.00831

CrossRef Full Text | Google Scholar

Rungwattana, K., and Hietz, P. (2017). Radial variation of wood functional traits reflect size-related adaptations of tree mechanics and hydraulics. Funct. Ecol. 32, 1–13.

Google Scholar

Skene, D. S. (1972). The kinetics of tracheid development in Tsuga canadensis Carr and its relation to tree vigour. Ann. Bot. 36, 179–187.

Google Scholar

Stovall, A. E. L., Shugart, H., and Xi, Y. (2019). Tree height explains mortality risk during an intense drought. Nat. Commun. 10:4385. doi: 10.1038/s41467-019-12380-6

CrossRef Full Text | Google Scholar

Tyree, M. T., and Ewers, F. W. (1991). The hydraulic architecture of trees and other woody plants. New Phytol. 119, 345–360.

Google Scholar

Vaganov, E. A., Hughes, M. K., and Shashkin, A. V. (2006). “Seasonal cambium activity and production of new xylem cells,” in Growth Dynamics of Conifer Tree Rings, Ecological Studies (Analysis and Synthesis), ed. E. A Vaganov, (Berlin: Springer), 71–104.

Google Scholar

von Arx, G., and Carrer, M. (2014). ROXAS – a new tool to build centuries-long tracheid-lumen chronologies in conifers. Dendrochronologia 32, 290–293.

Google Scholar

von Arx, G., Crivellaro, A., Prendin, A. L., Čufar, K., and Carrer, M. (2016). Quantitative wood anatomy - practical guidelines. Front. Plant Sci. 7:781. doi: 10.3389/fpls.2016.00781

CrossRef Full Text | Google Scholar

West, G. B., Brown, J. H., and Enquist, B. J. (1997). A general model for the origin of allometric scaling laws in biology. Science 276, 122–126. doi: 10.1126/science.276.5309.122

CrossRef Full Text | Google Scholar

Keywords: allometric effect, ontogenesis, quantitative wood anatomy, temporal stability, xylem hydraulic constraints

Citation: Piermattei A, von Arx G, Avanzi C, Fonti P, Gärtner H, Piotti A, Urbinati C, Vendramin GG, Büntgen U and Crivellaro A (2020) Functional Relationships of Wood Anatomical Traits in Norway Spruce. Front. Plant Sci. 11:683. doi: 10.3389/fpls.2020.00683

Received: 08 December 2019; Accepted: 30 April 2020;
Published: 26 May 2020.

Edited by:

Henry D. Adams, Oklahoma State University, United States

Reviewed by:

Peter Prislan, Slovenian Forestry Institute, Slovenia
Drew Peltier, Northern Arizona University, United States
Jean-Michel Leban, INRA Centre Nancy-Lorraine, France
Sabine Rosner, University of Natural Resources and Life Sciences Vienna, Austria

Copyright © 2020 Piermattei, von Arx, Avanzi, Fonti, Gärtner, Piotti, Urbinati, Vendramin, Büntgen and Crivellaro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Alma Piermattei, alma.piermattei@geog.cam.ac.uk