Unraveling Cuticle Formation, Structure, and Properties by Using Tomato Genetic Diversity

Abstract

The tomato (Solanum lycopersicum) fruit has a thick, astomatous cuticle that has become a model for the study of cuticle formation, structure, and properties in plants. Tomato is also a major horticultural crop and a long-standing model for research in genetics, fruit development, and disease resistance. As a result, a wealth of genetic resources and genomic tools have been established, including collections of natural and artificially induced genetic diversity, introgression lines of genome fragments from wild relatives, high-quality genome sequences, phenotype and gene expression databases, and efficient methods for genetic transformation and editing of target genes. This mini-review reports the considerable progresses made in recent years in our understanding of cuticle by using and generating genetic diversity for cuticle-associated traits in tomato. These include the synthesis of the main cuticle components (cutin and waxes), their role in the structure and properties of the cuticle, their interaction with other cell wall polymers as well as the regulation of cuticle formation. It also addresses the opportunities offered by the untapped germplasm diversity available in tomato and the current strategies available to exploit them.

Introduction

Cultivated tomato (Solanum lycopersicum L.) is a major horticultural crop that has long been a model for the Solanaceae crop species (tomato, potato, eggplant, pepper …) and for fleshy fruit development and disease resistance (Rothan et al., 2019). Tomato is very suitable for laboratory studies (grown in greenhouse, miniature cultivars, short life cycle, autogamy, and easy genetic transformation) has extensive genetic resources, high-quality reference genome (Tomato Genome Consortium, 2012), sequences of hundreds of accessions (Zhu et al., 2018; Gao et al., 2019; Alonge et al., 2020), and available phenotype and gene expression databases.¹ Thanks to these resources and tools, genes underlying trait variations can be identified and their relationships with phenotypic variations can be established through in planta functional analysis.

In the last decades, the use of natural diversity available in tomato, such as the ripening mutants ripening inhibitor (rin) or non-ripening (nor), has been instrumental to decipher the regulation of ripening (Klee and Giovannoni, 2011). These advances have been aided by the development of tools for functional analysis of target genes including RNA interference (RNAi) and CRISPR/Cas9 gene editing systems. The molecular determinants of fruit skin formation, which is an essential protective barrier against pests, pathogens, and water loss, have begun to be explored more recently (Vogg et al., 2004; Lemaire-Chamley et al., 2005; Hovav et al., 2007; Mintz-Oron et al., 2008).

Not surprisingly, since the cuticle is a major component of the fruit skin and is associated with a wide diversity of major breeding targets including fruit appearance (color, glossiness, regularity…) and properties (shelf-life, fungal resistance, and cracking; Bargel and Neinhuis, 2005; Petit et al., 2017; Lara et al., 2019), it has received considerable attention in recent years. In this field of study, tomato holds a prominent position among fleshy fruits because of its thick, astomatous, and easy-to-peel cuticle (Petit et al., 2017). Additionally, the wide diversity in cuticle architecture and composition found in wild tomato relatives (Yeats et al., 2012a; Halinski et al., 2015; Fernandez-Moreno et al., 2017) can be exploited for the discovery of novel cuticle-associated genes (Hovav et al., 2007; Zhang et al., 2021). Tomato has therefore become a model for the study of cuticle formation in plants. In the recent years, molecular determinants of cuticle have been identified and genetically altered lines have been produced, enabling the exploration of cuticle structure, properties, interactions with other cell wall components, and relationships with epidermal patterning. Table 1 summarizes the major findings on known tomato cuticle-associated genes, the pathway, or biological process in which they are involved and the main alterations produced by their mutation or de-regulation. This mini-review focuses on the strategies, resources, and tools used to reveal their role, providing examples, and considers future goals and developments.

Linking gene to phenotype can be done using approaches known as (i) reverse genetics in which the function of a target gene is mainly determined by analyzing the phenotype of mutant or deregulated lines and (ii) forward genetics, in which tomato genetic diversity is first screened for phenotypes-of-interest and the underlying genes and their function are then identified.

Exploring the Function of Cuticle-Associated Genes Through Reverse Genetics Approaches in Tomato

An obvious source of information on wax- and cutin-associated genes to study in tomato is the model plant Arabidopsis (Bernard and Joubes, 2013; Fich et al., 2016). Examples of the pertinence of this strategy are the conserved functions in Arabidopsis and tomato of the SHINE transcription factors (Shi et al., 2013; Al-Abdallat et al., 2014) and the recent demonstration that the SlABCG42 transporter, the tomato ortholog of the Arabidopsis ABCG PEC1 transporter, is functional and can transport various cutin precursors (Elejalde-Palmett et al., 2021).

For poorly characterized or unknown function genes, the information on where and when they are expressed provides the first cues on their possible role in cuticle formation. Gene expression has been explored in-depth in tomato fruit skin, a tissue which consists of the cuticle, the epidermis, and few layers of collenchyma cells underneath (Bargel and Neinhuis, 2005). Of special interest is the fruit expansion phase, before the onset of ripening, when rapid cuticle deposition occurs (Mintz-Oron et al., 2008; Petit et al., 2014). During the cell expansion phase, ploidy and volume of epidermal and sub-epidermal cells do not vary, whereas ploidy and cell volume undergo a dramatic increase in mesocarp cells (Joubès et al., 1999; Renaudin et al., 2017). In earlier studies, epidermis-expressed genes were identified via tissue-specific Expressed Sequence Tags (EST), microarrays (Lemaire-Chamley et al., 2005; Mintz-Oron et al., 2008), and proteome (Yeats et al., 2010). Several genes highlighted in these studies play prominent roles in cuticle formation, among which the CUTIN SYNTHASE (CUS1) catalyzing cutin polymerization (Girard et al., 2012; Yeats et al., 2012b) and the SlMIXTA-like regulating cuticle formation and epidermal patterning (Lashbrooke et al., 2015). Semi-quantitative RNA-seq coupled with laser microdissection (LMD) next allowed exhaustive inventory of gene transcripts expressed in plant tissues, including outer and inner fruit epidermis (Matas et al., 2011). Another original transcriptome-based approach used a chimera between two tomato species displaying genotype-specific E1 cell layer to provide a reference catalog of epidermis-specific genes (Filippis et al., 2013). Quantitative RNA-seq coupled with LMD later allowed the establishment of a quantitative tomato fruit gene atlas of developing fruit (Shinozaki et al., 2018). The resulting Tomato Expression Database (TEA) available at SGN² can be mined for clusters of genes co-expressed in specific developmental stages and cell types including inner and outer epidermis, which gives precious information on genes functionally linked to cuticle formation. Gene expression profiling can be further combined with various multi-omics technologies, typically metabolome (Fernandez-Moreno et al., 2016) and proteome (Szymanski et al., 2017), and with large scale sequencing of genetic diversity (Szymański et al., 2020) to assign putative functions to the genes and explore their role in cuticle formation. Novel functions revealed by biochemical approaches, e.g., the cutin:cutin-acid endo-transacylase (CCT) enzyme activity (Xin et al., 2021), may also ultimately lead to the isolation of the encoding gene.

Technologies used for the functional analysis of cuticle-associated genes (Table 1) include Virus-Induced Gene Silencing (VIGS; Ballester et al., 2010; España et al., 2014), stable gene overexpression (e.g., Shi et al., 2013) and silencing via RNA interference (RNAi; e.g., Girard et al., 2012; Lashbrooke et al., 2015) and artificial miRNA (Adato et al., 2009). More recently, the efficient CRISPR/Cas9 system, which allows genome editing of single or multiple target genes (Rothan et al., 2019), has been successfully used to validate the influence of a GA2-oxidase on cuticle formation and fruit firmness (Li et al., 2020). Recent development of genome editing technologies offers the possibility to specifically target cuticle genes in the fruit (Feder et al., 2020) when rapid cuticle deposition takes place (Mintz-Oron et al., 2008) by using the fruit- and expansion phase-specific promoter proPPC2 (Fernandez et al., 2009; Guillet et al., 2012). This further opens the perspective to specifically edit a cuticle gene in fruit epidermis, for instance by employing the promoter from the wax biosynthesis SlCER6 gene (Vogg et al., 2004) that drives reporter expression in both inner and outer fruit epidermis (Mintz-Oron et al., 2008).

Discovering Cuticle-Associated Genes Through Forward Genetics Approaches in Tomato

Though the list of cuticle-associated genes can be considerably shortened by mining existing databases and literature, their systematic analysis in planta may remain problematic because of gene redundancy, pleiotropic effect, etc. This may restrict the focus to known gene families, thus impeding the discovery of original gene functions. An alternative approach is to screen natural or artificially induced genetic diversity for phenotypes-of-interest, e.g., fruit surface defects induced by cutin deficiency (Isaacson et al., 2009; Petit et al., 2014), and then identify causal genetic variation.

Using Natural Genetic Diversity for Linking Cuticle Phenotype to Gene

Wild tomato species are an important source of genes lost during domestication of tomato (Gao et al., 2019). The main tomato germplasm repository, from which seeds can be ordered, is the Tomato Genetics Resource Center (TGRC).³ It includes the phenotypic description of wild tomato relatives and of thousands of cultivated tomato accessions carrying spontaneous or artificially induced mutations. Natural diversity can be screened for cuticle structure and composition (Yeats et al., 2012a; Halinski et al., 2015), transpirational water loss (Fich et al., 2020), and for mutations, such as positional sterile (ps), which causes organ fusion by affecting the wax decarboxylation pathway (Leide et al., 2011) or yellow (y) resulting from a SlMYB12 mutation (Adato et al., 2009; Ballester et al., 2010).

Most wild tomato relatives are easily crossed with tomato to generate segregating populations, e.g., the Solanum pimpinellifolium-derived population (Barraj Barraj et al., 2021), thus allowing the detection of cuticle Quantitative Trait Locus (QTL) and candidate genes. Genomic regions of wild species can be further fixed in an uniform S. lycopersicum genetic background to produce Introgression Lines (Ils) or Backcross Inbred Lines (BIls; Bazakos et al., 2017; Petit et al., 2017). The most extensively used ILs, derived from Solanum pennellii (Eshed and Zamir, 1995), enabled the rapid mapping of cuticle-associated QTLs and genes for leaf waxes (Ofner et al., 2016), wax alkane and amyrin content (Fernandez-Moreno et al., 2017), epidermal reticulation of green fruit (Cui et al., 2017), and fruit skin microfissuring (Zhang et al., 2021). Large scale transcriptome and metabolome analysis of fruit skin from 580 inbred lines further identified genes involved in flavonoid biosynthesis and fungal resistance (Szymański et al., 2020). Solanum habrochaites-derived lines allowed the identification of candidate genes for wax triterpenoids (Yeats et al., 2012a) and the isolation of the CWP1 gene of unknown function responsible for skin microfissuring (Hovav et al., 2007; Chechanovsky et al., 2019). Solanum chmielewskii ILs were used to identify a y mutant (Ballester et al., 2010).

The effects on cuticle formation of natural genetic variants found in cultivated tomato were also explored. STICKY PEEL encodes a HD-Zip IV protein that regulates epidermis metabolism and additional cuticle-associated traits (Kimbara et al., 2012; Nadakuduti et al., 2012) while WOOLY, a different HD-Zip IV protein, regulates trichome initiation and wax biosynthesis (Xiong et al., 2020). GA2-OXIDASE, which was identified through a fruit firmness QTL analysis, is unexpectedly related to a cuticle thickness QTL (Li et al., 2020) and connects cuticle formation with gibberellin (GA) signaling. In addition, because of the strong impact of cuticle properties on tomato postharvest storage (Saladié et al., 2007), lines harboring the well-studied non-ripening mutations rin and nor (Wang et al., 2020) as well as several long shelf-life tomato varieties [“Delayed Fruit Deterioration” (DFD) cultivar., de Penjar types] carrying allelic variants of NAC-NOR (e.g., alcobaca) have been characterized with respect to cuticle composition and properties (Saladié et al., 2007; Kosma et al., 2010; Kumar et al., 2018; Romero and Rose, 2019). The complex interplay between fruit development and ripening, epidermal patterning and metabolism, and cuticle formation and properties, which was revealed by these studies, was further supported by independent studies of the ripening regulators FUL1/FUL2 (Bemer et al., 2012) and TAGL1 (Giménez et al., 2015).

Using Artificially Induced Genetic Diversity for Linking Cuticle Phenotype to Gene

Several collections of artificially induced genetic diversity have been generated in cultivated tomato by transposon tagging (Meissner et al., 1997) or ethyl methanesulfonate (EMS) mutagenesis (Menda et al., 2004; Minoia et al., 2010; Saito et al., 2011; Just et al., 2013; Gupta et al., 2017). The two main mutagenized cultivars are the miniature Micro-Tom tomato (Meissner et al., 1997), which is well suited for laboratory use (Meissner et al., 1997; Just et al., 2013), and M82 (Menda et al., 2004), a processing tomato parent of the widely used S. pennellii ILs (Eshed and Zamir, 1995). Several cutin-deficient mutants were found in the M82 mutant collection (Isaacson et al., 2009) while more than 10 wax-altered and/or cutin-deficient glossy mutants were described in Micro-Tom (Petit et al., 2014). Many more mutants can be found by screening tomato mutant collections for obvious cuticle defects (Petit et al., 2014) and by browsing associated phenotypic databases (reviewed in Rothan et al., 2016), such as TOMATOMA⁴ (Saito et al., 2011). EMS mutant collections can also be screened by TILLING (Targeting Induced Local Lesions IN Genomes; Okabe et al., 2011) though mutated alleles of target genes are now efficiently generated by gene editing (Rothan et al., 2019).

As detailed in Table 1, mutant collections have been instrumental for: (i) discovering novel gene functions (SlCUS1; Yeats et al., 2012b); (ii) isolating allelic variants of genes implicated in wax biosynthesis (SlCER6; Vogg et al., 2004; Smirnova et al., 2013), cutin biosynthesis (SlCYP86A69; Shi et al., 2013; SlGPAT6; Petit et al., 2016) and polymerization (SlCUS1; Petit et al., 2014), and regulation of flavonoid biosynthesis (SlMYB12; Fernandez-Moreno et al., 2016); and (iii) deciphering the regulation of epidermis (HD-Zip IV; Isaacson et al., 2009).

Concluding Remarks and Perspectives

Thanks to its thick astomatous fruit cuticle that is easy to study and the wealth of mutants and lines with altered expression of cuticle-related genes already available, tomato provides an excellent model for deciphering the molecular determinants of cuticle formation, structure, and properties. As detailed in Table 1, reverse and forward genetics approaches led to the isolation of tomato genes involved in several cuticle-associated biosynthetic pathways including wax biosynthesis (SlCER6, SLTTS1, and SlTTS2); cutin biosynthesis (SlCYP86A69 and SlGPAT6), transport (SlABCG36/42), and polymerization (SlCUS1 and SlDCR); and flavonoid biosynthesis (SlCHS1 and SlCHS2). Besides genes encoding various transcription factors (SlSHN1 and SlSHN3, SlMIXTA-like, two HD-Zip IV, SlMYB31, and SlMYB12) or related to ABA (LYCOPENE CYCLASE b and SlPP2C3) and GA (GA2-OXIDASE) hormonal pathways were shown to regulate cuticle formation and its coordination with epidermal patterning.

Altogether these studies demonstrated the interest of both reverse and forward genetic approaches for discovering novel cuticle gene functions and for generating new plant material to study in-depth cuticle architecture, properties, and interaction with other cell wall components. To face new challenges in cuticle studies, several research avenues can be envisaged in the next future. Analysis of key cuticle genes should be extended to additional family members not yet analyzed for their role in cuticle deposition. An example is that of the GDSL-domain family to which belongs the cutin synthase CUS1 enzyme, whose function was discovered and confirmed through convergent studies in tomato (Table 1). The expression of different CUS1 homologs is deregulated, sometimes oppositely, in several cutin-deficient mutants (Lashbrooke et al., 2015; Petit et al., 2016). By analogy with the recently demonstrated role of GDSL-domain proteins in suberin formation (Ursache et al., 2021), CUS1 homologs (Yeats et al., 2014) may fulfill opposite functions of cutin polymerization and degradation in fruit skin, thereby adapting cuticle formation to rapid fruit growth. Particular attention should also be given to enzymes from the poorly explored phenolic pathway in the epidermis which has evolutionary conserved roles in cuticle properties (Philippe et al., 2020b; Kriegshauser et al., 2021) and to proteins involved in the modification of cell wall polysaccharides (Philippe et al., 2020a). Cell wall enzymes and other proteins likely play central roles in cuticle structure and properties and in the coordination of cuticle deposition with organ development and epidermal patterning (Philippe et al., 2020b). So far, few studies have linked cuticle formation in tomato with hormones, except for ABA (Diretto et al., 2020; Liang et al., 2021) and GA (Li et al., 2020). The role of ABA is not surprising considering the importance of ABA signaling in the response to various stresses (Curvers et al., 2010; Liang et al., 2021), as established in tomato leaves (Martin et al., 2017). More unexpected is the role of GA, the effect of which on cuticle formation is far from being understood (Li et al., 2020). In view of its numerous roles in plant defense and development (Fenn and Giovannoni, 2021), ethylene is also a likely candidate for regulating cuticle formation in tomato fruit. The selection of new “guilt-by-association” target genes for these pathways and processes can be considerably aided by literature mining and/or exploitation of various genomic data (transcriptome, proteome, and metabolome) including co-expression analyses across various fruit cell types and developmental stages (Shinozaki et al., 2018) and across various plant species (Lashbrooke et al., 2016).

In addition to the approaches described above, which often requires previous knowledge of the gene, pathway or process to be targeted, the exploration of untapped genetic diversity, for example, tomato landraces (Conesa et al., 2020), is a way to get off the beaten tracks. This can be very rewarding but also lead to the isolation of challenging genes with no obvious or demonstrated link to the cuticle (Li et al., 2020), or even unknown function (Hovav et al., 2007). One bottleneck in such approach is the phenotyping of large collections (Petit et al., 2017). It can be easy for obvious cuticular defects (Hovav et al., 2007), more challenging for less evident changes (Petit et al., 2014), and complex when low or medium throughput technologies are required, e.g., for wax and cutin monomer analyses (Fernandez-Moreno et al., 2017). In the recent years, high resolution genotyping, whole genome sequencing-based strategies, such as mapping-by-sequencing (MBS) or QTL-seq (Garcia et al., 2016; Bazakos et al., 2017), accelerated gene isolation in tomato (Rothan et al., 2019). However, though identifying the causal mutation can be relatively straightforward in EMS mutants (Garcia et al., 2016), isolating causal genetic variations from natural diversity can be complex since it usually requires crossing the genotype-of-interest with a distant genotype. The subsequent co-segregation in the progeny of numerous cuticle traits unrelated to the trait-of-interest may make phenotyping very difficult and even prohibit high resolution mapping of the genetic variant (Rothan et al., 2016). Association mapping has emerged in the last decade as a powerful tool to discover linkages between gene polymorphism and variations in fruit traits by exploring natural genetic diversity. In tomato, sequencing of hundreds of accessions combined with fruit phenotyping pinpointed genetic variations associated with changes in fruit size, flavor, and color among which SlMYB12 polymorphisms responsible for pink fruit color (Zhu et al., 2018). More recently, pan-genome analysis of hundreds of wild and cultivated tomato accessions further uncovered numerous unknown genes and genetic variations underlying fruit traits (Gao et al., 2019; Alonge et al., 2020). Phenotyping selected panels of sequenced accessions for cuticle-associated traits should yield numerous genetic variations controlling cuticle and skin formation and properties, and help identifying new functions involved in these processes. In addition to the genetic control of cuticle formation, the epigenetic regulation of wax and cutin biosynthesis will be worth exploring in the near future, as a preliminary study has shown that altering histone methylation status has a profound effect on the composition of tomato fruit cuticle (Boureau et al., 2016; Table 1).

Accession Numbers

Sequence data from this article can be found in the SGN database under accession numbers found in Table 1.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

• 1

  AdatoA.MandelT.Mintz-OronS.VengerI.LevyD.YativM.et al. (2009). Fruit-surface flavonoid accumulation in tomato is controlled by a SlMYB12-regulated transcriptional network. PLoS Genet.5:e1000777. doi: 10.1371/journal.pgen.1000777

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 2

  Al-AbdallatA. M.Al-DebeiH. S.AyadJ. Y.HasanS. (2014). Over-expression of SlSHN1 gene improves drought tolerance by increasing cuticular wax accumulation in tomato. Int. J. Mol. Sci.15, 19499–19515. doi: 10.3390/ijms151119499

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 3

  AlongeM.WangX.BenoitM.SoykS.PereiraL.ZhangL.et al. (2020). Major impacts of widespread structural variation on gene expression and crop improvement in tomato. Cell182, 145.e23–161.e23. doi: 10.1016/j.cell.2020.05.021

  • CrossRef
  • Google Scholar

• 4

  BallesterA. R.MolthoffJ.de VosR.HekkertB. L.OrzaezD.Fernández-MorenoJ. P.et al. (2010). Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit color. Plant Physiol.152, 71–84. doi: 10.1104/pp.109.147322

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 5

  BargelH.NeinhuisC. (2005). Tomato (Lycopersicon esculentum mill.) fruit growth and ripening as related to the biomechanical properties of fruit skin and isolated cuticle. J. Exp. Bot.56, 1049–1060. doi: 10.1093/jxb/eri098

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 6

  Barraj BarrajR.SegadoP.Moreno-GonzálezR.HerediaA.Fernández-MuñozR.DomínguezE. (2021). Genome-wide QTL analysis of tomato fruit cuticle deposition and composition. Hortic. Res.8:113. doi: 10.1038/s41438-021-00548-5

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 7

  BazakosC.HanemianM.TrontinC.Jiménez-GómezJ. M.LoudetO. (2017). New strategies and tools in quantitative genetics: how to go from the phenotype to the genotype. Annu. Rev. Plant Biol.68, 435–455. doi: 10.1146/annurev-arplant-042916-040820

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 8

  BemerM.KarlovaR.BallesterA. R.TikunovY. M.BovyA. G.Wolters-ArtsM.et al. (2012). The tomato FRUITFULL homologs TDR4/FUL1 and BP7/FUL2 regulate ethylene-independent aspects of fruit ripening. Plant Cell24, 4437–4451. doi: 10.1105/tpc.112.103283

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 9

  BernardA.JoubesJ. (2013). Arabidopsis cuticular waxes: advances in synthesis, export and regulation. Prog. Lipid Res.52, 110–129. doi: 10.1016/j.plipres.2012.10.002

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 10

  BoureauL.How-KitA.TeyssierE.DrevensekS.RainieriM.JoubèsJ.et al. (2016). A CURLY LEAF homologue controls both vegetative and reproductive development of tomato plants. Plant Mol. Biol.90, 485–501. doi: 10.1007/s11103-016-0436-0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 11

  BuxdorfK.RubinskyG.BardaO.BurdmanS.AharoniA.LevyM. (2014). The transcription factor SlSHINE3 modulates defense responses in tomato plants. Plant Mol. Biol.84, 37–47. doi: 10.1007/s11103-013-0117-1

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 12

  ChechanovskyN.HovavR.FrenkelR.FaigenboimA.EselsonY.PetreikovM.et al. (2019). Low temperature upregulates cwp expression and modifies alternative splicing patterns, increasing the severity of cwp-induced tomato fruit cuticular microfissures. Hortic. Res.6:122. doi: 10.1038/s41438-019-0204-9

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 13

  ConesaM. À.Fullana-PericàsM.GranellA.GalmésJ. (2020). Mediterranean long shelf-life landraces: an untapped genetic resource for tomato improvement. Front. Plant Sci.10:1651. doi: 10.3389/fpls.2019.01651

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 14

  CuiL.QiuZ.WangZ.GaoJ.GuoY.HuangZ.et al. (2017). Fine mapping of a gene (ER4.1) that causes epidermal reticulation of tomato fruit and characterization of the associated transcriptome. Front. Plant Sci.8:1254. doi: 10.3389/fpls.2017.01254

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 15

  CurversK.SeifiH.MouilleG.de RyckeR.AsselberghB.Van HeckeA. (2010). Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea. Plant Physiol.154, 847–860. doi: 10.1104/pp.110.158972

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 16

  DirettoG.FruscianteS.FabbriC.SchauerN.BustaL.WangZ.et al. (2020). Manipulation of β-carotene levels in tomato fruits results in increased ABA content and extended shelf life. Plant Biotechnol. J.18, 1185–1199. doi: 10.1111/pbi.13283

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 17

  Elejalde-PalmettC.Martinez San SegundoI.GarroumI.CharrierL.De BellisD.MuccioloA.et al. (2021). ABCG transporters export cutin precursors for the formation of the plant cuticle. Curr. Biol.31, 2111.e9–2123.e9. doi: 10.1016/j.cub.2021.02.056

  • CrossRef
  • Google Scholar

• 18

  EshedY.ZamirD. (1995). An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics141, 1147–1162. doi: 10.1093/genetics/141.3.1147

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 19

  EspañaL.Heredia-GuerreroJ. A.Reina-PintoJ. J.Fernández-MuñozR.HerediaA.DomínguezE. (2014). Transient silencing of CHALCONE SYNTHASE during fruit ripening modifies tomato epidermal cells and cuticle properties. Plant Physiol.166, 1371–1386. doi: 10.1104/pp.114.246405

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 20

  FawkeS.TorodeT. A.GoglevaA.FichE. A.SørensenI.YunusovT.et al. (2019). Glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis. New Phytol.223, 1547–1559. doi: 10.1111/nph.15846

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 21

  FederA.JensenS.WangA.CourtneyL.MiddletonL.Van EckJ.et al. (2020). Tomato fruit as a model for tissue-specific gene silencing in crop plants. Hortic. Res.7:142. doi: 10.1038/s41438-020-00363-4

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 22

  FennM. A.GiovannoniJ. J. (2021). Phytohormones in fruit development and maturation. Plant J.105, 446–458. doi: 10.1111/tpj.15112

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 23

  FernandezA. I.VironN.AlhagdowM.KarimiM.JonesM.AmsellemZ.et al. (2009). Flexible tools for gene expression and silencing in tomato. Plant Physiol.151, 1729–1740. doi: 10.1104/pp.109.147546

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 24

  Fernandez-MorenoJ. P.Levy-SamohaD.MalitskyS.MonforteA. J.OrzaezD.AharoniA.et al. (2017). Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population. J. Exp. Bot.68, 2703–2716. doi: 10.1093/jxb/erx134

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 25

  Fernandez-MorenoJ. P.TzfadiaO.FormentJ.PresaS.RogachevI.MeirS.et al. (2016). Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor. Plant Physiol.171, 1821–1836. doi: 10.1104/pp.16.00282

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 26

  FichE. A.FisherJ.ZamirD.RoseJ. (2020). Transpiration from tomato fruit occurs primarily via trichome-associated transcuticular polar pores. Plant Physiol.184, 1840–1852. doi: 10.1104/pp.20.01105

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 27

  FichE. A.SegersonN. A.RoseJ. K. (2016). The plant polyester cutin: biosynthesis, structure, and biological roles. Annu. Rev. Plant Biol.67, 207–233. doi: 10.1146/annurev-arplant-043015-111929

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 28

  FilippisI.Lopez-CobolloR.AbbottJ.ButcherS.BishopG. J. (2013). Using a periclinal chimera to unravel layer-specific gene expression in plants. Plant J.75, 1039–1049. doi: 10.1111/tpj.12250

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 29

  GaoL.GondaI.SunH.MaQ.BaoK.TiemanD. M.et al. (2019). The tomato pan-genome uncovers new genes and a rare allele regulating fruit flavor. Nat. Genet.51, 1044–1051. doi: 10.1038/s41588-019-0410-2

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 30

  GarciaV.BresC.JustD.FernandezL.TaiF. W. J.MauxionJ. P.et al. (2016). Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing. Nat. Protoc.11, 2401–2418. doi: 10.1038/nprot.2016.143

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 31

  GiménezE.DominguezE.PinedaB.HerediaA.MorenoV.LozanoR.et al. (2015). Transcriptional activity of the MADS box ARLEQUIN/TOMATO AGAMOUS-LIKE1 gene is required for cuticle development of tomato fruit. Plant Physiol.168, 1036–1048. doi: 10.1104/pp.15.00469

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 32

  GirardA. L.MounetF.Lemaire-ChamleyM.GaillardC.ElmorjaniK.VivancosJ.et al. (2012). Tomato GDSL1 is required for cutin deposition in the fruit cuticle. Plant Cell24, 3119–3134. doi: 10.1105/tpc.112.101055

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 33

  GuilletC.Aboul-SoudM. A.Le MennA.VironN.PribatA.GermainV.et al. (2012). Regulation of the fruit-specific PEP carboxylase SlPPC2 promoter at early stages of tomato fruit development. PLoS One7:e36795. doi: 10.1371/journal.pone.0036795

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 34

  GuptaP.ReddaiahB.SalavaH.UpadhyayaP.TyagiK.SarmaS.et al. (2017). Next-generation sequencing (NGS)-based identification of induced mutations in a doubly mutagenized tomato (Solanum lycopersicum) population. Plant J.92, 495–508. doi: 10.1111/tpj.13654

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 35

  HalinskiL. P.KalkowskaM.KalkowskiM.PiorunowskaJ.TopolewskaA.StepnowskiP. (2015). Cuticular wax variation in the tomato (Solanum lycopersicum L.), related wild species and their interspecific hybrids. Biochem. Syst. Ecol.60, 215–224. doi: 10.1016/j.bse.2015.04.030

  • CrossRef
  • Google Scholar

• 36

  HerediaA.Heredia-GuerreroJ. A.DomínguezE. (2015). CHS silencing suggests a negative cross-talk between wax and flavonoid pathways in tomato fruit cuticle. Plant Signal. Behav.10:e1019979. doi: 10.1080/15592324.2015.1019979

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 37

  HovavR.ChehanovskyN.MoyM.JetterR.SchafferA. A. (2007). The identification of a gene (Cwp1), silenced during Solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit. Plant J.52, 627–639. doi: 10.1111/j.1365-313X.2007.03265.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 38

  IsaacsonT.KosmaD. K.MatasA. J.BudaG. J.HeY.YuB.et al. (2009). Cutin deficiency in the tomato fruit cuticle consistently affects resistance to microbial infection and biomechanical properties, but not transpirational water loss. Plant J.60, 363–377. doi: 10.1111/j.1365-313X.2009.03969.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 39

  JoubèsJ.PhanT. H.JustD.RothanC.BergouniouxC.RaymondP.et al. (1999). Molecular and biochemical characterization of the involvement of cyclin-dependent kinase A during the early development of tomato fruit. Plant Physiol.121, 857–869. doi: 10.1104/pp.121.3.857

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 40

  JustD.GarciaV.FernandezL.BresC.MauxionJ. P.PetitJ.et al. (2013). Micro-tom mutants for functional analysis of target genes and discovery of new alleles in tomato. Plant Biotechnol.30, 1–7. doi: 10.5511/plantbiotechnology.13.0622a

  • CrossRef
  • Google Scholar

• 41

  KimbaraJ.YoshidaM.ItoH.HosoiK.KusanoM.KobayashiM.et al. (2012). A novel class of sticky peel and light green mutations causes cuticle deficiency in leaves and fruits of tomato (Solanum lycopersicum). Planta236, 1559–1570. doi: 10.1007/s00425-012-1719-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 42

  KimbaraJ.YoshidaM.ItoH.KitagawaM.TakadaW.HayashiK.et al. (2013). Inhibition of CUTIN DEFICIENT 2 causes defects in cuticle function and structure and metabolite changes in tomato fruit. Plant Cell Physiol.54, 1535–1548. doi: 10.1093/pcp/pct100

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 43

  KleeH. J.GiovannoniJ. J. (2011). Genetics and control of tomato fruit ripening and quality attributes. Annu. Rev. Genet.45, 41–59. doi: 10.1146/annurev-genet-110410-132507

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 44

  KosmaD. K.ParsonsE. P.IsaacsonT.LüS.RoseJ. K.JenksM. A. (2010). Fruit cuticle lipid composition during development in tomato ripening mutants. Physiol. Plant.139, 107–117. doi: 10.1111/j.1399-3054.2009.01342.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 45

  KriegshauserL.KnospS.GrienenbergerE.TatsumiK.GütleD. D.SørensenI.et al. (2021). Function of the HYDROXYCINNAMOYL-CoA:SHIKIMATE HYDROXYCINNAMOYL TRANSFERASE is evolutionarily conserved in embryophytes. Plant Cell.33, 1472–1491. doi: 10.1093/plcell/koab044

  • CrossRef
  • Google Scholar

• 46

  KumarR.TamboliV.SharmaR.SreelakshmiY. (2018). NAC-NOR mutations in tomato Penjar accessions attenuate multiple metabolic processes and prolong the fruit shelf life. Food Chem.259, 234–244. doi: 10.1016/j.foodchem.2018.03.135

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 47

  LaraI.HerediaA.DomínguezE. (2019). Shelf life potential and the fruit cuticle: the unexpected player. Front. Plant Sci.10:770. doi: 10.3389/fpls.2019.00770

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 48

  LashbrookeJ.AdatoA.LotanO.AlkanN.TsimbalistT.RechavK.et al. (2015). The tomato MIXTA-like transcription factor coordinates fruit epidermis conical cell development and cuticular lipid biosynthesis and assembly. Plant Physiol.169, 2553–2571. doi: 10.1104/pp.15.01145

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 49

  LashbrookeJ.CohenH.Levy-SamochaD.TzfadiaO.PanizelI.ZeislerV.et al. (2016). MYB107 and MYB9 homologs regulate suberin deposition in angiosperms. Plant Cell28, 2097–2116. doi: 10.1105/tpc.16.00490

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 50

  LeideJ.HildebrandtU.ReussingK.RiedererM.VoggG. (2007). The developmental pattern of tomato fruit wax accumulation and its impact on cuticular transpiration barrier properties: effects of a deficiency in a beta-ketoacyl-coenzyme A synthase (LeCER6). Plant Physiol.144, 1667–1679. doi: 10.1104/pp.107.099481

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 51

  LeideJ.HildebrandtU.VoggG.RiedererM. (2011). The positional sterile (ps) mutation affects cuticular transpiration and wax biosynthesis of tomato fruits. J. Plant Physiol.168, 871–877. doi: 10.1016/j.jplph.2010.11.014

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 52

  Lemaire-ChamleyM.PetitJ.GarciaV.JustD.BaldetP.GermainV.et al. (2005). Changes in transcriptional profiles are associated with early fruit tissue specialization in tomato. Plant Physiol.139, 750–769. doi: 10.1104/pp.105.063719

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 53

  LiR.SunS.WangH.WangK.YuH.ZhouZ.et al. (2020). FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato. Nat. Commun.11:5844. doi: 10.1038/s41467-020-19705-w

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 54

  LiangB.SunY.WangJ.ZhengY.ZhangW.XuY.et al. (2021). Tomato protein phosphatase 2C influences the onset of fruit ripening and fruit glossiness. J. Exp. Bot.72, 2403–2418. doi: 10.1093/jxb/eraa593

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 55

  MartinL.RomeroP.FichE. A.DomozychD. S.RoseJ. (2017). Cuticle biosynthesis in tomato leaves is developmentally regulated by abscisic acid. Plant Physiol.174, 1384–1398. doi: 10.1104/pp.17.00387

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 56

  MartinL. B.SherwoodR. W.NicklayJ. J.YangY.Muratore-SchroederT. L.AndersonE. T.et al. (2016). Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor. Proteomics16, 2081–2094. doi: 10.1002/pmic.201500450

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 57

  MatasA. J.YeatsT. H.BudaG. J.ZhengY.ChatterjeeS.TohgeT.et al. (2011). Tissue- and cell-type specific transcriptome profiling of expanding tomato fruit provides insights into metabolic and regulatory specialization and cuticle formation. Plant Cell23, 3893–3910. doi: 10.1105/tpc.111.091173

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 58

  MeissnerR.JacobsonY.MelamedS.LevyatuvS.ShalevG.AshriA.et al. (1997). A new model system for tomato genetics. Plant J.12, 1465–1472. doi: 10.1046/j.1365-313x.1997.12061465.x

  • CrossRef
  • Google Scholar

• 59

  MendaN.SemelY.PeledD.EshedY.ZamirD. (2004). In silico screening of a saturated mutation library of tomato. Plant J.38, 861–872. doi: 10.1111/j.1365-313X.2004.02088.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 60

  MinoiaS.PetrozzaA.D'OnofrioO.PironF.MoscaG.SozioG.et al. (2010). A new mutant genetic resource for tomato crop improvement by TILLING technology. BMC Res. Notes3:69. doi: 10.1186/1756-0500-3-69

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 61

  Mintz-OronS.MandelT.RogachevI.FeldbergL.LotanO.YativM.et al. (2008). Gene expression and metabolism in tomato fruit surface tissues. Plant Physiol.147, 823–851. doi: 10.1104/pp.108.116004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 62

  MoreiraC.BentoA.PaisJ.PetitJ.EscórcioR.CorreiaV. G.et al. (2020). An ionic liquid extraction that preserves the molecular structure of cutin shown by nuclear magnetic resonance. Plant Physiol.184, 592–606. doi: 10.1104/pp.20.01049

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 63

  NadakudutiS. S.PollardM.KosmaD. K.AllenC.Jr.OhlroggeJ. B.BarryC. S. (2012). Pleiotropic phenotypes of the sticky peel mutant provide new insight into the role of CUTIN DEFICIENT2 in epidermal cell function in tomato. Plant Physiol.159, 945–960. doi: 10.1104/pp.112.198374

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 64

  OfnerI.LashbrookeJ.PlebanT.AharoniA.ZamirD. (2016). Solanum pennellii backcross inbred lines (BILs) link small genomic bins with tomato traits. Plant J.87, 151–160. doi: 10.1111/tpj.13194

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 65

  OkabeY.AsamizuE.SaitoT.MatsukuraC.AriizumiT.BrèsC.et al. (2011). Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from micro-tom mutant libraries. Plant Cell Physiol.52, 1994–2005. doi: 10.1093/pcp/pcr134

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 66

  PetitJ.BresC.JustD.GarciaV.MauxionJ. P.MarionD.et al. (2014). Analyses of tomato fruit brightness mutants uncover both cutin-deficient and cutin-abundant mutants and a new hypomorphic allele of GDSL lipase. Plant Physiol.164, 888–906. doi: 10.1104/pp.113.232645

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 67

  PetitJ.BresC.MauxionJ. P.BakanB.RothanC. (2017). Breeding for cuticle-associated traits in crop species: traits, targets, and strategies. J. Exp. Bot.68, 5369–5387. doi: 10.1093/jxb/erx341

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 68

  PetitJ.BresC.MauxionJ. P.TaiF. W.MartinL. B.FichE. A.et al. (2016). The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis. Plant Physiol.171, 894–913. doi: 10.1104/pp.16.00409

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 69

  PhilippeG.GaillardC.PetitJ.GeneixN.DalgalarrondoM.BresC.et al. (2016). Ester cross-link profiling of the cutin polymer of wild-type and cutin synthase tomato mutants highlights different mechanisms of polymerization. Plant Physiol.170, 807–820. doi: 10.1104/pp.15.01620

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 70

  PhilippeG.GeneixN.PetitJ.GuillonF.SandtC.RothanC.et al. (2020a). Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides. New Phytol.226, 809–822. doi: 10.1111/nph.16402

  • CrossRef
  • Google Scholar

• 71

  PhilippeG.SørensenI.JiaoC.SunX.FeiZ.DomozychD. S.et al. (2020b). Cutin and suberin: assembly and origins of specialized lipidic cell wall scaffolds. Curr. Opin. Plant Biol.55, 11–20. doi: 10.1016/j.pbi.2020.01.008

  • CrossRef
  • Google Scholar

• 72

  RenaudinJ. P.DelucheC.ChenicletC.ChevalierC.FrangneN. (2017). Cell layer-specific patterns of cell division and cell expansion during fruit set and fruit growth in tomato pericarp. J. Exp. Bot.68, 1613–1623. doi: 10.1093/jxb/erx058

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 73

  RomeroP.RoseJ. (2019). A relationship between tomato fruit softening, cuticle properties and water availability. Food Chem.295, 300–310. doi: 10.1016/j.foodchem.2019.05.118

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 74

  RothanC.BresC.GarciaV.JustD. (2016) in Tomato Resources for Functional Genomics. eds. CausseM.GiovannoniJ.BouzayenM.ZouineM. (Berlin Heidelberg: Springer), 75–94.

  • Google Scholar

• 75

  RothanC.DioufI.CausseM. (2019). Trait discovery and editing in tomato. Plant J.97, 73–90. doi: 10.1111/tpj.14152

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 76

  SaitoT.AriizumiT.OkabeY.AsamizuE.Hiwasa-TanaseK.FukudaN. (2011). TOMATOMA: a novel tomato mutant database distributing micro-tom mutant collections. Plant Cell Physiol.52, 283–296. doi: 10.1093/pcp/pcr004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 77

  SaladiéM.MatasA. J.IsaacsonT.JenksM. A.GoodwinS. M.NiklasK. J.et al. (2007). A reevaluation of the key factors that influence tomato fruit softening and integrity. Plant Physiol.144, 1012–1028. doi: 10.1104/pp.107.097477

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 78

  SegadoP.Heredia-GuerreroJ. A.HerediaA.DomínguezE. (2020). Cutinsomes and CUTIN SYNTHASE1 function sequentially in tomato fruit cutin deposition. Plant Physiol.183, 1622–1637. doi: 10.1104/pp.20.00516

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 79

  ShiJ. X.AdatoA.AlkanN.HeY.LashbrookeJ.MatasA. J.et al. (2013). The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning. New Phytol.197, 468–480. doi: 10.1111/nph.12032

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 80

  ShinozakiY.NicolasP.Fernandez-PozoN.MaQ.EvanichD. J.ShiY.et al. (2018). High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening. Nat. Commun.9, 364. doi: 10.1038/s41467-017-02782-9

  • CrossRef
  • Google Scholar

• 81

  SmirnovaA.LeideJ.RiedererM. (2013). Deficiency in a very-long-chain fatty acid β-ketoacyl-coenzyme a synthase of tomato impairs microgametogenesis and causes floral organ fusion. Plant Physiol.161, 196–209. doi: 10.1104/pp.112.206656

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 82

  SzymańskiJ.BocobzaS.PandaS.SonawaneP.CárdenasP. D.LashbrookeJ.et al. (2020). Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response. Nat. Genet.52, 1111–1121. doi: 10.1038/s41588-020-0690-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 83

  SzymanskiJ.LevinY.SavidorA.BreitelD.Chappell-MaorL.HeinigU.et al. (2017). Label-free deep shotgun proteomics reveals protein dynamics during tomato fruit tissues development. Plant J.90, 396–417. doi: 10.1111/tpj.13490

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 84

  Tomato Genome Consortium (2012). The tomato genome sequence provides insights into fleshy fruit evolution. Nature485, 635–641. doi: 10.1038/nature11119

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 85

  UrsacheR.De Jesus Vieira TeixeiraC.Dénervaud TendonV.GullyK.De BellisD.Schmid-SiegertE.et al. (2021). GDSL-domain proteins have key roles in suberin polymerization and degradation. Nat. Plants7, 353–364. doi: 10.1038/s41477-021-00862-9

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 86

  VoggG.FischerS.LeideJ.EmmanuelE.JetterR.LevyA. A.et al. (2004). Tomato fruit cuticular waxes and their effects on transpiration barrier properties: functional characterization of a mutant deficient in a very-long-chain fatty acid beta-ketoacyl-CoA synthase. J. Exp. Bot.55, 1401–1410. doi: 10.1093/jxb/erh149

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 87

  WangR.AngenentG. C.SeymourG.de MaagdR. A. (2020). Revisiting the role of master regulators in tomato ripening. Trends Plant Sci.25, 291–301. doi: 10.1016/j.tplants.2019.11.005

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 88

  XinA.FeiY.MolnarA.FryS. C. (2021). Cutin:cutin-acid endo-transacylase (CCT), a cuticle-remodelling enzyme activity in the plant epidermis. Biochem. J.478, 777–798. doi: 10.1042/BCJ20200835

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 89

  XiongC.XieQ.YangQ.SunP.GaoS.LiH.et al. (2020). WOOLLY, interacting with MYB transcription factor MYB31, regulates cuticular wax biosynthesis by modulating CER6 expression in tomato. Plant J.103, 323–337. doi: 10.1111/tpj.14733

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 90

  YeatsT. H.BudaG. J.WangZ.ChehanovskyN.MoyleL. C.JetterR.et al. (2012a). The fruit cuticles of wild tomato species exhibit architectural and chemical diversity, providing a new model for studying the evolution of cuticle function. Plant J.69, 655–666. doi: 10.1111/j.1365-313X.2011.04820.x

  • CrossRef
  • Google Scholar

• 91

  YeatsT. H.HoweK. J.MatasA. J.BudaG. J.ThannhauserT. W.RoseJ. K. (2010). Mining the surface proteome of tomato (Solanum lycopersicum) fruit for proteins associated with cuticle biogenesis. J. Exp. Bot.61, 3759–3771. doi: 10.1093/jxb/erq194

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 92

  YeatsT. H.HuangW.ChatterjeeS.ViartH. M.ClausenM. H.StarkR. E.et al. (2014). Tomato cutin deficient 1 (CD1) and putative orthologs comprise an ancient family of cutin synthase-like (CUS) proteins that are conserved among land plants. Plant J.77, 667–675. doi: 10.1111/tpj.12422

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 93

  YeatsT. H.MartinL. B.ViartH. M.IsaacsonT.HeY.ZhaoL.et al. (2012b). The identification of cutin synthase: formation of the plant polyester cutin. Nat. Chem. Biol.8, 609–611. doi: 10.1038/nchembio.960

  • CrossRef
  • Google Scholar

• 94

  ZhangC.WangT.LiJ.ZhangD.XieQ.MunirS.et al. (2021). Functional gain of fruit netted-cracking in an introgression line of tomato with higher expression of the FNC gene. Front. Agric. Sci. Eng.8, 280–291. doi: 10.15302/J-FASE-2020374

  • CrossRef
  • Google Scholar

• 95

  ZhuG.WangS.HuangZ.ZhangS.LiaoQ.ZhangC.et al. (2018). Rewiring of the fruit metabolome in tomato breeding. Cell172, 249.e12–261.e12. doi: 10.1016/j.cell.2017.12.019

  • CrossRef
  • Google Scholar