Abstract
Idioblasts are defined by abnormal shapes, sizes, and contents that are different from neighboring cells. Myrosin cells are Brassicales-specific idioblasts and accumulate a large amount of thioglucoside glucohydrolases (TGGs, also known as myrosinases) in their vacuoles. Myrosinases convert their substrates, glucosinolates, into toxic compounds when herbivories and pests attack plants. In this review, we highlight the similarities and differences between myrosin cells and vascular cells/guard cells (GCs) because myrosin cells are distributed along vascular cells, especially the phloem parenchyma, and myrosin cells share the master transcription factor FAMA with GCs for their cell differentiation. In addition, we analyzed the overlap of cell type-specific genes between myrosin cells and GCs by using published single-cell transcriptomics (scRNA-seq) data, suggesting significant similarities in the gene expression patterns of these two specialized cells.
Physiological Roles of the Myrosinase–Glucosinolate System
Idioblasts are cells with abnormal shapes, sizes, and contents (proteins and metabolites) (Foster, 1956). Myrosin cells are Brassicales-specific idioblasts. In model plants, Arabidopsis thaliana, myrosin cells are distributed along veins in the aerial parts of the plants without hypocotyls. Myrosin cells are named after storage proteins, myrosinases, which are called “myrosin grain” and thioglucoside glucohydrolase (TGG) (Rask et al., 2000; Andreasson et al., 2001). The substrates of myrosinases are glucosinolates, which are sulfur-rich compounds in plants and accumulate at other types of specialized cells called S-cells (Koroleva et al., 2000; Shroff et al., 2008; Burow and Halkier, 2017; Nintemann et al., 2018). Under normal developmental conditions, enzymes and substrates never meet each other because they are separated into different cell types. After the cell collapses by the attack of herbivories, myrosinase cleaves the bond between sulfur and glucose in glucosinolates to produce the toxic compounds, isothiocyanates (Shirakawa and Hara-Nishimura, 2018). This defense strategy was named “mustard oil bomb” and is one of the critical factors for the habitat range of Brassicales plants (Prasad et al., 2012). In a model plant, A. thaliana, two kinds of myrosinases, TGG1 and TGG2, are stored in myrosin cells. Double knockout mutants of TGG1 and TGG2 have exhibited weak resistance against herbivories compared with the wild-type (Barth and Jander, 2006). Unlike TGG2, it is well known that TGG1 is also expressed in guard cells (GCs) (Zhao et al., 2008). However, the role of the myrosinase–glucosinolate system in GCs was unknown until recently. Salehin et al. (2019) showed that the myrosinase–glucosinolate system is required for the closure of stomata under drought conditions. These results suggested that the myrosinase–glucosinolate system has different functions in two different specialized cells.
Thioglucoside glucohydrolases are defined by the conserved glutamine that is required for binding to glucosinolates. The conserved residue is replaced by glutamic acid in the atypical myrosinases, PENETRATION2 (PEN2) and PYK10 (Matsushima et al., 2003; Bednarek et al., 2009). They prefer indole glucosinolates to aliphatic glucosinolates. For more detailed information about the function and evolution of atypical myrosinases, see Nakano et al. (2014); Pastorczyk and Bednarek (2016), and Nakano et al. (2017).
Anti-Myrosinase–Glucosinolate Strategies and Reuse of Glucosinolates
During evolution, herbivories evolved the resistance and secondary use of the myrosinase–glucosinolates system. Diamondback moth is a crucifer specialist insect and produces glucosinolate sulfatase (GSS) to detoxify glucosinolates (Ratzka et al., 2002). GSS hydrolases glucosinolates to produce desulfo-glucosinolates, which myrosinases cannot cleave. The evolution of GSS during the battle between insects and plants is an open question.
Other insects accumulate plant toxins, glucosinolates, for defense against predators. The specialist herbivorous insect Phyllotreta striolata (flea beetle) ingests glucosinolates and has myrosinases that may cleave glucosinolates from plants (Beran et al., 2014). Recently, it was reported that the horseradish flea beetle Phyllotreta armoraciae uses a sugar transporter as a glucosinolate transporter to transfer glucosinolates from the excretory system to the hemolymph (Yang et al., 2021). It is an interesting question how and why flea beetles start to use glucosinolates for their defense strategies.
Myrosin Cells Versus Vascular Cells
Myrosin cells are distributed along veins, especially the phloem (Shirakawa and Hara-Nishimura, 2018; Shimada et al., 2018). Myrosin cells contact directly with phloem parenchyma. During development, myrosin cells never encounter veins, resulting in two networks, the network of veins and myrosin cells, which are wired coordinately (parallel organization and alignment). This observation provoked the question of whether myrosin cells differentiate from vascular precursor cells (procambium cells). Two groups compared the spatiotemporal expression patterns of a myrosin cell reporter and a procambium reporter and showed that myrosin cells do not differentiate from procambium cells and, rather, directly differentiate from ground meristem cells, which are stem cell-like cells in inner tissue (Li and Sack, 2014; Shirakawa et al., 2016b). Ground meristem cells are also mother cells of mesophyll cells and procambium cells. How plants coordinate the development of myrosin cells and vascular cells remains an open question. Polar auxin transport (PAT) is required for the proper development of both vascular cells and myrosin cells, suggesting that auxin may coordinate the development of both cell types. Interestingly, mutants of SYNTAXIN OF PLANTS 22 (SYP22) exhibited a less vascular network than wild-type and, in contrast, had more myrosin cells than the wild-type (Ueda et al., 2006; Shirakawa et al., 2009, 2010, 2014a). SYP22 is required for PAT in leaf primordia through the endocytosis of the auxin efflux carrier PIN-FORMED 1 (PIN1) (Shirakawa et al., 2014c). syp22 failed to canalize auxin resulting the abnormal distribution of auxin. Taken together, we hypothesized that high levels of auxin induce the vascular differentiation and low levels of auxin induce the differentiation of myrosin cells. Different dosages of auxin may regulate the development of two specialized cells.
Why do myrosin cells distribute along leaf veins? First, S-cells are distributed along the primary veins. Plants need to develop myrosin cells close to S-cells to efficiently produce toxic compounds when herbivories eat them. This may work as a costless defense system, protecting the lifeline of plants without the loss of photosynthetic organs. Second, myrosin cells may need to communicate with vascular cells, especially phloem cells, to exchange nutrients and metabolites. Consistent with this hypothesis, SUGAR TRANSPORTER PROTEIN 8 is specifically expressed in myrosin cells (Rottmann et al., 2018). Like the wiring of the vein network and myrosin cell network, two networks, the nervous system and blood vessel network, are wired in animals and are called “neurovascular links” (Walchli et al., 2015). The wiring of networks of vascular cells and myrosin cells (named myrovascular links) may be a good model for research on the coordination of the two networks. Future studies may identify new roles of myrosin cells in the context of communication with vascular cells, which may be independent of S-cells.
Myrosin Cells Versus Guard Cells
The basic helix–loop–helix transcription factor FAMA was identified as a master transcription factor for the differentiation of myrosin cells from ground meristem cells (Li and Sack, 2014; Shirakawa et al., 2014b, 2016a). FAMA is also known as the master TF for the transition from guard mother cells (GMCs) into GCs (Ohashi-Ito and Bergmann, 2006). Sister TFs, SPEECHLESS (SPCH) and MUTE, regulate the transition from protodermal cells into meristemoids and the transition from meristemoids into GMCs, respectively (MacAlister et al., 2007; Pillitteri et al., 2007; Lau and Bergmann, 2012; Han and Torii, 2016). Recently, it was shown that MUTE directly activates FAMA in stomatal lineage cells (Han et al., 2018). However, in inner tissues, MUTE is not required for the expression of FAMA, suggesting that other TF(s) activate the expression of FAMA in inner tissues (Shirakawa et al., 2014b). The distribution of FAMA-expressing cells in inner tissues was changed by treatment with PAT inhibitors and in mutants of PAT, including syp22 (Li and Sack, 2014; Shirakawa et al., 2014c). Auxin response factors (ARFs) may activate FAMA in inner tissues.
The downstream FAMA has been well studied in the stomatal lineage (Hachez et al., 2011; Weimer et al., 2018). In the stomatal lineage, FAMA inhibits the ectopic divisions of GMCs and promotes the differentiation of GCs (Ohashi-Ito and Bergmann, 2006). One of the D-type cyclins, CYCD7, is directly repressed by FAMA to inhibit ectopic divisions of GMCs (Figure 1A; Weimer et al., 2018). Although other downstream factors were identified by transcriptome analysis (Hachez et al., 2011), it was still unclear which direct targets of FAMA differentiate from GMCs to GCs. One of the candidates is DNA-binding with one finger (DOF) TF, STOMATAL CARPENTER 1. SCAP1 is upregulated in inducible FAMA-overexpression lines (Hachez et al., 2011). SCAP1 is expressed from young GCs to mature GCs, suggesting that the expression window of SCAP1 fits the later expression window of FAMA (Figure 1A; Negi et al., 2013; Lopez-Anido et al., 2021). In addition, half of GCs in scap1 mutants exhibited skewed morphologies (Negi et al., 2013). SCAP1 is a potential direct target of FAMA in young GCs. However, the stomatal phenotypes of scap1 were much weaker than those of fama. Other direct target(s) of FAMA must exist and cooperatively promote the differentiation of GCs with SCAP1.
FIGURE 1
In addition to TFs, a leucine-rich repeat receptor-like kinase (MUSTACHES/MUS), a structural protein [FUSED OUTER CUTICULAR LEDGE1 (FOCL1)] and enzymes [POLYGALACTURONASE INVOLVED IN EXPANSION3 (PGX3) and OCCLUSION OF STOMATAL PORE 1 (OSP1)] that are involved in the formation of pores were identified over a decade (Keerthisinghe et al., 2015; Hunt et al., 2017; Rui et al., 2017; Tang et al., 2020). mus exhibited skewed GC and unopen GC, suggesting that MUS receives unknown ligands that coordinate the bilateral symmetry of GC. focl1 and osp1 showed similar phenotypes in that the pores of stomata were covered by membranous cuticular materials. FOCL1 is a secreted cell wall structural protein, and OSP1 is a GDSL lipase. It is an interesting question how FOCL1 and OSP1 interact genetically and biochemically. PGX3 is required for the formation of the proper pore size. It is still an open question whether the expression of these factors is regulated by FAMA.
Compared with stomata, key factor(s) of the differentiation of myrosin cells, which are downstream of FAMA, have not yet been identified (Figure 1B). Only one of the myrosinases, TGG1, has recently been reported to be a direct target of FAMA (Feng et al., 2021). Overall, key factor(s) that promote the differentiation of two specialized cells after FAMA remain enigmatic. In addition to downstream factors of FAMA, several interaction partners of FAMA have been identified (Mair et al., 2019). One of them, SCREAMs, is required for the differentiation of stomata and myrosin cells (Kanaoka et al., 2008; Shirakawa et al., 2014b). Other factors may have specific developmental/physiological functions in one of two specialized cells.
Commonality Between Myrosin Cells and Guard Cells
The FAMA-SCRM complex is a common master regulator of the differentiation of both myrosin cells and GCs. Therefore, it is expected that the two cell types may share gene expression patterns. Very recently, transcriptome analysis of Arabidopsis leaves was performed at single-cell resolution (scRNA-seq) (Berrío et al., 2021). Surprisingly, during the analysis, the unsupervised clustering of scRNA-seq data recognized myrosin cells and GCs as a single cluster. In addition, then, combined with the known markers (an epidermal marker was only expressed at GCs, not at myrosin cells), the authors divided them into two different clusters. Consistent with the previous experiment with reporter lines, TGG1 was expressed in both cell types, and TGG2 was exclusively expressed in myrosin cells in scRNA-seq, suggesting that scRNA-seq with manual clustering successfully separated the two cell types.
These results suggest that the gene expression patterns of the two types of specialized cells may be quite similar. Indeed, we compared cell type-specific genes of two specialized cells, and the analysis revealed that the commonly expressed genes were more than 50% of genes expressed in each cell type (myrosin cells 54%; GCs 64%) (Figure 2). This result indicates that unknown common function(s) may exist in two specialized cells. In addition, some myrosin cells expressed vascular markers, suggesting that myrosin cells can be segmentized into more small groups and that some of them may have features of vascular cells (Berrío et al., 2021). Taken together, scRNA-seq is a powerful tool for research on cell fate determination and differentiation.
FIGURE 2
Perspectives
In this review, we discuss the functional and developmental link between myrosin cells and vascular cells and GCs. The link between myrosin cells and vascular cells is hypothesized from anatomical research, and the unexpected link between myrosin cells and GCs is hypothesized from the discovery of master transcription factors. These hypotheses were partly supported by recent scRNA-seq data (Figure 2). These hypotheses may be connected with findings of the new physiological role of myrosin cells.
The downstream factors of FAMA must provide important information to answer this question. During this decade, some direct targets of FAMA were identified. However, the whole network of FAMA downstream is still largely unknown. By using inducible overexpression lines, RNA-seq analysis with sampling at multiple time points may be useful because FAMA is continuously expressed during the development of GCs (Lopez-Anido et al., 2021), and by using such methods, downstream targets of SPCH and MUTE have been identified (Lau et al., 2014; Han et al., 2018). In addition to classical transcriptome analysis, scRNA-seq is a useful tool not only for the identification of cell type-specific genes but also for the reconstruction of cell lineages (Lopez-Anido et al., 2021). Combined with stage-specific fluorescent reporters, fluorescence-activated cell sorting (FACS), and scRNA-seq, it may be possible to reveal the gradual change in gene expression patterns of myrosin cells from the beginning to the maturation of cell differentiation (lineage tracing).
Moreover, in the plant research field, small molecules with various biological functions have been recently identified (Nemhauser and Torii, 2016; Ziadi et al., 2017; Shirakawa et al., 2021). For example, the chemical compound bubblin increased the number of GCs (Sakai et al., 2017). By transient treatment and dosage control, small molecules can overcome the genetic redundancy and lethality of gene functions. It could be possible to identify the molecules that manipulate the number and distribution of myrosin cells and convert the identity of myrosin cells into GCs/vascular cells and vice versa.
The physiological function and developmental program of idioblast myrosin cells are largely unknown. Future works will shed light on the comprehensive molecular network of the function and development of myrosin cells. This information may be connected with the research field of applied plant science.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Statements
Author contributions
MS and MT analyzed the scRNA-seq data and described the figures. MS and TI wrote the manuscript. All authors read and approved the final version of the manuscript.
Funding
This work was supported by a grant from the JSPS KAKENHI Grant-in-Aid for Scientific Research C (19K06722), the JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (20H05416), the Takeda Science Foundation, the Kato Memorial Bioscience Foundation, and the Ohsumi Frontier Science Foundation to MS, as well as a grant from the JSPS KAKENHI Grant-in-Aid for Scientific Research A (20H00470), the JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (20H04888), and the JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (19H04865) to TI.
Acknowledgments
We thank lab members for helpful discussions.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AndreassonE.JorgensenL. B.HoglundA. S.MeijerJ. (2001). Different myrosinase and idioblast distribution in Arabidopsis and Brassica napus.Plant Physiol.1271750–1763. 10.1104/pp.010334
2
BarthC.JanderG. (2006). Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense.Plant J.46549–562. 10.1111/j.1365-313X.2006.02716.x
3
BednarekP.Pislewska-BednarekM.SvatosA.SchneiderB.DoubskyJ.MansurovaM.et al (2009). A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense.Science323101–106. 10.1126/science.1163732
4
BeranF.PauchetY.KunertG.ReicheltM.WielschN.VogelH.et al (2014). Phyllotreta striolata flea beetles use host plant defense compounds to create their own glucosinolate-myrosinase system.Proc. Natl. Acad. Sci. U. S. A.1117349–7354. 10.1073/pnas.1321781111
5
BerríoR. T.VerstaenK.VandammeN.PevernagieJ.AchonI.DuyseJ. V.et al (2021). Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells.Plant Physiol.[Epub Online ahead of print]. 10.1093/plphys/kiab489
6
BurowM.HalkierB. A. (2017). How does a plant orchestrate defense in time and space? Using glucosinolates in Arabidopsis as case study.Curr. Opin. Plant Biol.38142–147. 10.1016/j.pbi.2017.04.009
7
FengQ.LiL.LiuY.ShaoX.LiX. (2021). Jasmonate regulates the FAMA/mediator complex subunit 8-THIOGLUCOSIDE GLUCOHYDROLASE 1 cascade and myrosinase activity.Plant Physiol.187963–980. 10.1093/plphys/kiab283
8
FosterA. S. (1956). Plant idioblasts: remarkable examples of cell specializaiton.Protoplasma46184–193.
9
HachezC.Ohashi-ItoK.DongJ.BergmannD. C. (2011). Differentiation of Arabidopsis guard cells: analysis of the networks incorporating the basic helix-loop-helix transcription factor, FAMA.Plant Physiol.1551458–1472. 10.1104/pp.110.167718
10
HanS. K.QiX.SugiharaK.DangJ. H.EndoT. A.MillerK. L.et al (2018). MUTE directly orchestrates cell-stare switch and the single symmetric division to create stomata.Dev. Cell.45303–315. 10.1016/j.devcel.2018.04.010
11
HanS. K.ToriiK. U. (2016). Lineage-specific stem cells, signals and asymmetries during stomatal development.Development1431259–1270. 10.1242/dev.127712
12
HuntL.AmsburyS.BaillieA.MovahediM.MitchellA.AfsharinafarM.et al (2017). Formation of the stomatal outer cuticular ledge requires a guard cell wall proline-rich protein.Plant Physiol.174689–699. 10.1104/pp.16.01715
13
KanaokaM. M.PillitteriL. J.FujiiH.YoshidaY.BogenschutzN. L.TakabayashiJ.et al (2008). SCREAM/ICE1 and SCREAM2 specify three cell-state transitional steps leading to Arabidopsis stomatal differentiation.Plant Cell201775–1785. 10.1105/tpc.108.060848
14
KeerthisingheS.NadeauJ. A.LucasJ. R.NakagawaT.SackF. D. (2015). The Arabidopsis leucine-rich repeat receptor-like kinase MUSTACHES enforces stomatal bilateral symmetry in Arabidopsis.Plant J.81684–694. 10.1111/tpj.12757
15
KorolevaO. A.DaviesA.DeekenR.ThorpeM. R.TomosA. D.HedrichR.et al (2000). Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk.Plant Physiol.124599–608. 10.1104/pp.124.2.599
16
LauO. S.BergmannD. C. (2012). Stomatal development: a plant’s perspective on cell polarity, cell fate transitions and intercellular communication.Development1393683–3692. 10.1242/dev.080523
17
LauO. S.DaviesK. A.ChangJ.AdrianJ.RoweM. H.BallengerC. E.et al (2014). Direct roles of SPEECHLESS in the specification of stomatal self-renewing cells.Science3451605–1609. 10.1126/science.1256888
18
LiM.SackF. D. (2014). Myrosin idioblast cell fate and development are regulated by the Arabidopsis transcription factor FAMA, the Auxin pathway, and vesicular trafficking.Plant Cell264053–4066. 10.1105/tpc.114.129726
19
Lopez-AnidoC. B.VatenA.SmootN. K.SharmaN.GuoV.GongY.et al (2021). Single-cell resolution of lineage trajectories in the Arabidopsis stomatal lineage and developing leaf.Dev. Cell561043–1055. 10.1016/j.devcel.2021.03.014
20
MacAlisterC. A.Ohashi-ItoK.BergmannD. C. (2007). Transcription factor control of asymmetric cell divisions that establish the stomatal lineage.Nature445537–540. 10.1038/nature05491
21
MairA.XuS. L.BranonT. C.TingA. Y.BergmannD. C. (2019). Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID.Elife8:e47864.
22
MatsushimaR.KondoM.NishimuraM.Hara-NishimuraI. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a b-glucosidase with an ER-retention signal in Arabidopsis.Plant Cell Physiol.33493–502. 10.1046/j.1365-313x.2003.01636.x
23
NakanoR. T.YamadaK.BednarekP.NishimuraM.Hara-NishimuraI. (2014). ER bodies in plants of the Brassicales order: biogenesis and association with innate immunity.Front. Plant Sci.5:73. 10.3389/fpls.2014.00073
24
NakanoR. T.YamadaK.BednarekP.NishimuraM.Hara-NishimuraI. (2017). PYK10 myrosinase reveals a functional coordination between endoplasmic reticulum bodies and glucosinolates in Arabidopsis thaliana.Plant J.89204–220. 10.1111/tpj.13377
25
NegiJ.MoriwakiK.KonishiM.YokoyamaR.NakanoT.KusumiK.et al (2013). A Dof transcription factor, SCAP1, is essential for the development of functional stomata in Arabidopsis.Curr. Biol.23479–484. 10.1016/j.cub.2013.02.001
26
NemhauserJ. L.ToriiK. U. (2016). Plant synthetic biology for molecular engineering of signalling and development.Nat. Plants2:16010. 10.1038/nplants.2016.10
27
NintemannS. J.HunzikerP.AndersenT. G.SchulzA.BurowM.HalkierB. A. (2018). Localization of the glucosinolate biosynthetic enzymes reveals distinct spatial patterns for the biosynthesis of indole and aliphatic glucosinolates.Physiol. Plant163138–154. 10.1111/ppl.12672
28
Ohashi-ItoK.BergmannD. C. (2006). Arabidopsis FAMA controls the final proliferation/differentiation switch during stomatal development.Plant Cell182493–2505. 10.1105/tpc.106.046136
29
PastorczykM.BednarekP. (2016). The function of glucosinolates and related metabolites in plant innate immunity.Adv. Bot. Res.80171–198.
30
PillitteriL. J.SloanD. B.BogenschutzN. L.ToriiK. U. (2007). Termination of asymmetric cell division and differentiation of stomata.Nature445501–505. 10.1038/nature05467
31
PrasadK. V.SongB. H.Olson-ManningC.AndersonJ. T.LeeC. R.SchranzM. E.et al (2012). A gain-of-function polymorphism controlling complex traits and fitness in nature.Science3371081–1084. 10.1126/science.1221636
32
RaskL.AndreassonE.EkbomB.ErikssonS.PontoppidanB.MeijerJ. (2000). Myrosinase: gene family evolution and herbivore defense in Brassicaceae.Plant Mol. Biol.4293–113. 10.1007/978-94-011-4221-2_5
33
RatzkaA.VogelH.KliebensteinD. J.Mitchell-OldsT.KroymannJ. (2002). Disarming the mustard oil bomb.Proc. Natl. Acad. Sci. U. S. A.9911223–11228. 10.1073/pnas.172112899
34
RottmannT.KleblF.SchneiderS.KischkaD.RuscherD.SauerN.et al (2018). Sugar Transporter STP7 Specificity for L-Arabinose and D-Xylose Contrasts with the Typical Hexose Transporters STP8 and STP12.Plant Physiol.1762330–2350. 10.1104/pp.17.01493
35
RuiY.XianC.YiH.KandemirB.WangJ. Z.PuriV. M.et al (2017). POLYGALACTURONASE INVOLVED IN EXPANSION3 Functions in Seedling Development, Rosette Growth, and Stomatal Dynamics in Arabidopsis thaliana.Plant Cell292413–2432. 10.1105/tpc.17.00568
36
SakaiY.SuganoS. S.KawaseT.ShirakawaM.ImaiY.KawamotoY.et al (2017). The chemical compound bubblin induces stomatal mispatterning in Arabidopsis by disrupting the intrinsic polarity of stomatal lineage cells.Development144499–506. 10.1242/dev.145458
37
SalehinM.LiB.TangM.KatzE.SongL.EckerJ. R.et al (2019). Auxin-sensitive Aux/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels.Nat. Commun.10:4021. 10.1038/s41467-019-12002-1
38
ShimadaT.TakagiJ.IchinoT.ShirakawaM.Hara-NishimuraI. (2018). Plant Vacuoles.Annu. Rev. Plant Biol.69123–145.
39
ShirakawaM.Hara-NishimuraI. (2018). Specialized vacuoles of myrosin cells: chemical defense strategy in brassicales plants.Plant Cell Physiol.591309–1316. 10.1093/pcp/pcy082
40
ShirakawaM.MorisakiY.GanE. S.SatoA.ItoT. (2021). Identification of a devernalization inducer by chemical screening approaches in Arabidopsis thaliana.Front. Plant Sci.12:634068. 10.3389/fpls.2021.634068
41
ShirakawaM.UedaH.KoumotoY.FujiK.NishiyamaC.KohchiT.et al (2014a). CONTINUOUS VASCULAR RING (COV1) is a trans-golgi network-localized membrane protein required for golgi morphology and vacuolar protein sorting.Plant Cell Physiol.55764–772. 10.1093/pcp/pct195
42
ShirakawaM.UedaH.ShimadaT.KohchiT.Hara-NishimuraI. (2014c). Myrosin Cell Development Is Regulated by Endocytosis Machinery and PIN1 Polarity in Leaf Primordia of Arabidopsis thaliana.Plant Cell264448–4461. 10.1105/tpc.114.131441
43
ShirakawaM.UedaH.NaganoA. J.ShimadaT.KohchiT.Hara-NishimuraI. (2014b). FAMA is an essential component for the differentiation of two distinct cell types, myrosin cells and guard cells, in Arabidopsis.Plant Cell264039–4052. 10.1105/tpc.114.129874
44
ShirakawaM.UedaH.ShimadaT.Hara-NishimuraI. (2016a). FAMA: a molecular link between stomata and myrosin cells.Trends Plant Sci.21861–871. 10.1016/j.tplants.2016.07.003
45
ShirakawaM.UedaH.ShimadaT.Hara-NishimuraI. (2016b). Myrosin cells are differentiated directly from ground meristem cells and are developmentally independent of the vasculature in Arabidopsis leaves.Plant Signal. Behav.11:e1150403. 10.1080/15592324.2016.1150403
46
ShirakawaM.UedaH.ShimadaT.KoumotoY.ShimadaT. L.KondoM.et al (2010). Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.Plant J.64924–935. 10.1111/j.1365-313X.2010.04394.x
47
ShirakawaM.UedaH.ShimadaT.NishiyamaC.Hara-NishimuraI. (2009). Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution.Plant Cell Physiol.501319–1328. 10.1093/pcp/pcp076
48
ShroffR.VergaraF.MuckA.SvatosA.GershenzonJ. (2008). Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense.Proc. Natl. Acad. Sci. U. S. A.1056196–6201. 10.1073/pnas.0711730105
49
TangJ.YangX.XiaoC.LiJ.ChemY.LiR.et al (2020). GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation.New Phytol.2281880–1896. 10.1111/nph.16741
50
UedaH.NishiyamaC.ShimadaT.KoumotoY.HayashiY.KondoM.et al (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells.Plant Cell Physiol.47164–175. 10.1093/pcp/pci232
51
WalchliT.WackerA.FreiK.RegliL.SchwabM. E.HoerstrupS. P.et al (2015). Wiring the vascular network with neural cues: a CNS perspective.Neuron87271–296. 10.1016/j.neuron.2015.06.038
52
WeimerA. K.MatosJ. L.SharmaN.PatellF.MurrayJ. A. H.DewitteW.et al (2018). Lineage- and stage-specific expressed CYCD7;1 coordinates the single symmetric division that creates stomatal guard cells.Development145:dev160671. 10.1242/dev.160671
53
YangZ. L.Nour-EldinH. H.HannigerS.ReicheltM.CrocollC.SeitzF.et al (2021). Sugar transporters enable a leaf beetle to accumulate plant defense compounds.Nat. Commun.12:658. 10.1038/s41467-021-22982-8
54
ZhaoZ.ZhangW.StanleyB. A.AssmannS. M. (2008). Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways.Plant Cell203210–3226. 10.1105/tpc.108.063263
55
ZiadiA.UchidaN.KatoH.HisamatsuR.SatoA.HagiharaS.et al (2017). Discovery of synthetic small molecules that enhance the number of stomata: C–H functionalization chemistry for plant biology.Chem. Commun.539632–9635. 10.1039/c7cc04526c
Summary
Keywords
cell differentiation, FAMA, glucosinolate, guard cell, myrosin cell, myrosinase, vascular cell
Citation
Shirakawa M, Tanida M and Ito T (2022) The Cell Differentiation of Idioblast Myrosin Cells: Similarities With Vascular and Guard Cells. Front. Plant Sci. 12:829541. doi: 10.3389/fpls.2021.829541
Received
05 December 2021
Accepted
17 December 2021
Published
10 January 2022
Volume
12 - 2021
Edited by
Takumi Higaki, Kumamoto University, Japan
Reviewed by
Yuki Kondo, Kobe University, Japan
Updates
Copyright
© 2022 Shirakawa, Tanida and Ito.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Makoto Shirakawa, shirakawa@bs.naist.jpToshiro Ito, itot@bs.naist.jp
This article was submitted to Plant Development and EvoDevo, a section of the journal Frontiers in Plant Science
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.