Abstract
Little is known about the molecular basis of the influence of external carbon/nitrogen (C/N) ratio and other abiotic factors on phytohormones regulation during seed germination and plant developmental processes, and the identification of elements that participate in this response is essential to understand plant nutrient perception and signaling. Sugars (sucrose, glucose) and nitrate not only act as nutrients but also as signaling molecules in plant development. A connection between changes in auxin transport and nitrate signal transduction has been reported in Arabidopsis thaliana through the NRT1.1, a nitrate sensor and transporter that also functions as a repressor of lateral root growth under low concentrations of nitrate by promoting auxin transport. Nitrate inhibits the elongation of lateral roots, but this effect is significantly reduced in abscisic acid (ABA)-insensitive mutants, what suggests that ABA might mediate the inhibition of lateral root elongation by nitrate. Gibberellin (GA) biosynthesis has been also related to nitrate level in seed germination and its requirement is determined by embryonic ABA. These mechanisms connect nutrients and hormones signaling during seed germination and plant development. Thus, the genetic identification of the molecular components involved in nutrients-dependent pathways would help to elucidate the potential crosstalk between nutrients, nitric oxide (NO) and phytohormones (ABA, auxins and GAs) in seed germination and plant development. In this review we focus on changes in C and N levels and how they control seed germination and plant developmental processes through the interaction with other plant growth regulators, such as phytohormones.
Introduction
A seed needs to integrate all the signals that represent its nutritional status in order to achieve germination when the appropriate nutrients conditions are present. Only when the right hormonal response takes place, seed germination is induced and seedlings become mature plants.
Nutrients are also known to influence seedling development and some processes may be mediated by the absolute levels of a particular sugar, such as glucose () or sucrose (), whereas other processes may be related to metabolic events associated with the presence of carbohydrates at high concentrations, rather than the presence of high concentrations of carbohydrate per se (). Moreover, sugar-responsive pathways exhibit crosstalk with nitrogen-responsive pathways (; ). Therefore, C/N balance seems to be crucial for the regulation of gene expression by carbohydrates and nitrogen (; ; Zhuo et al., 1999; Zheng, 2009). In spite of the importance of C/N balance signaling, the number of genes so far implicated in this process is scarce. Among these genes, NRT2.1 and NRT2.2 are high-affinity nitrate transporters in roots, particularly important under non-limiting nitrogen growth conditions (). GLR1.1, a putative glutamate receptor 1.1, connects carbon and nitrogen metabolism, ABA metabolism and water stress response in Arabidopsis (). OSU1/QUA2/TSD2-encoded putative methyltransferase may act in cell wall biogenesis () and is necessary for a normal response to C/N balance (). Different targets of OSU1/QUA2/TSD2 would regulate pectin biosynthesis and responses to C/N balance, thus connecting cell wall biogenesis and C/N balance response (Zheng, 2009). OSU1 seems to work by down-modulating different pathways resulting in high or low C/N responses. Alternatively it could work on a single pathway regulating several transcription factors in response to C/N imbalance conditions (Zheng, 2009). Finally, it has been demonstrated the involvement of specific ABA and SnRK1s signaling pathways in C/N response under ABI1 regulation (). Therefore, for each identified molecular player it is essential to check whether there is a signal representing C/N balance signal or the C/N balance sensing network just results from the crosstalk between carbohydrates and nitrogen pathways.
Genes involved in metabolism, protein synthesis and degradation, RNA metabolism, signal transduction and hormones (auxin, gibberellin, cytokinin, ethylene, abscisic acid and brassinosteroids) pathways may play important roles in the C/N balance or ratio response, and nutrients crosstalk has also been reported (; ; ; ; ). Additionally, C/N signaling systems are subject to a “matrix effect” in which downstream responses are dependent upon cell-type plus developmental, metabolic, and/or environmental conditions ().
This review is focused on the mechanisms underlying the regulation of seed dormancy, seed germination and seedling development by carbon and nitrogen nutrient balance. Specific C- and N-regulatory pathways and C/N interaction pathways controlling these developmental processes will be described and discussed.
N-Control of Development
Seed Dormancy and Germination
Germination is classically described as a triphasic process determined by water relations. Phase I: the seed imbibes and resumes metabolism. Phase II: water uptake by the seed reaches a plateau and stays in a dormant state. Phase III: water uptake is resumed and the radicle emerges from the seed ().
The depth of seed dormancy was inversely correlated to seed nitrate content, “endogenous nitrate” (). Production of dormant seeds was inhibited in plants grown in high concentration of nitrate (50 mM) during seed maturation when compared to plants grown under standard conditions (10 mM nitrate). The nia1/nia2 double mutant, which accumulates nitrate under standard conditions, produces seeds that are less dormant than WT seeds under identical conditions (). Nitrate uptake by NRT1.1 was suggested to play a relevant role in dormancy regulation (). One of the genes encoding nitrate reductase, NR1, was highly expressed under conditions that break dormancy, including cold, light and nitrate (). Unlike NR1, expression of NiR gene may depend on nitrate. However, dormancy release was not accompanied by increased transcript abundance of none of the seven (putative) glutamine synthetase (GS1) genes. Control of nitrate assimilation at the (post) translational level was also analyzed, and the nitrate reductase mutant G′4-3 of Arabidopsis, with just 0.5% of the nitrate reductase activity of the corresponding wild type, was even less dormant than the wild type (). Thus nitrate uptake and reduction to nitrite seems to generate a signal for dormancy breaking instead of just being a source of an essential nutrient.
Nitrate can enhance ABA catabolism and inhibit ABA synthesis (). Addition of nitrate to dormant seeds resulted in less ABA content and supress de novo synthesis. Seed dormancy was broken by nitrate, nitrite and CN- through NO production and altered ABA sensitivity (). As shown by , regulation of ABA degradation is more important than regulation of ABA synthesis for the control of seed dormancy by exogenous nitrate. They proposed that CYP707A2, a gene involved in ABA catabolism, could play a role in the control of seed ABA content by endogenous nitrate (Figure 1). In addition, other genes like NCED9 must be involved in the control of dormancy by nitrate, since cyp707a2 mutant seeds behaved as the wild-type under 10 mM nitrate.
FIGURE 1
In Arabidopsis, there are two genes encoding nitrate reductase (NR), NIA1 and NIA2. NIA1, which is expressed at much lower level than NIA2, seems to be responsible for NO production in the context of ABA signaling (). Recently, have demonstrated that NO production in Arabidopsis is mostly associated to NO biosynthesis mediated by NIA/NR and AtNOA1 (Nitric Oxide-Associated1). Seed dormancy, hypersensitivity to ABA during seed germination and establishment, as well as dehydration resistance, all showed a good correlation with an increasing deficiency in NO of nia1nia2, noa1-2, and nia1nia2noa1-2 plants.
In Arabidopsis and barley, NO seems to be an endogenous regulator of seed germination (Figure 1), since it can disrupt seed dormancy (, ,; ). A similar function for NO was suggested by in warm-season C4-grasses. Switchgrass seeds responded positively to NO, and NO could disrupt the residual dormancy found in cold-stratified switchgrass seeds.
NO or KCN vapors induced germination in seeds lacking a testa, suggesting that NO was perceived in the aleurone cells or in the embryo. Aleurone layer perceived NO and responded to it because vacuolation was inhibited by the NO scavenger (), strongly suggesting that Arabidopsis aleurone cells synthesize NO. There are no evidences that NO is produced enzymatically. On the contrary, NO seems to be generated in the apoplast of the aleurone cell layer by non-enzymatic reduction of nitrite at acidic pH in germinating seeds, and catechin would also stimulate the production of NO from nitrite with a maximum at pH 3–4 (,). Nitrite either entering the grain from soil solution or released by the embryo axis, the scutellum, or the aleurone layer to the apoplast/endosperm cavity, would result in NO production (), and apoplast of both GA- and ABA-treated Hordeum vulgare aleurone layers can produce NO from nitrite. NO would be an ideal signal for coordinating the activities of the embryo axis, scutellum, and aleurone layer in real time. Removal or damage of the aleurone layer resulted in embryo growth, demonstrating that one function of the Arabidopsis aleurone layer is to maintain the dormancy of imbibed seeds.
In Arabidopsis thaliana seeds, sodium nitro-prusside, cyanide, nitrate and nitrite decreased dormancy and the NO scavenger c-PTIO effectively promoted the maintenance of seed dormancy (). NO was required to complete dormancy loss initiated by nitrate or nitrite, and cPTIO maintained dormancy in nitrite- and nitrate-treated seeds. Experimental data from showed that exogenous gaseous NO is sufficient to disrupt seed dormancy. have also suggested that, in Arabidopsis, the transcriptome anticipates growth initiation during dormancy-breaking. An increased NR1 transcript abundance would anticipate nitrate assimilation during seedling growth. In switchgrass, however, seed germination was not significantly stimulated by nitrite or nitrate alone, and ferrocyanide was more effective than cyanide ().
GAs promote germination () and GA deficient mutants fail to germinate (). The importance of ABA/GA balance for germination is supported by the fact that the reduced dormancy of some of the ABA mutants was linked to a lower GA-requirement for germination (). GAs promote rapid degradation of DELLA proteins (intracellular repressors of GA responses), which leads to activation of the signaling pathway, and the comparison of gene expression in ga1-3. In DELLA-domain protein-deficient mutants (gai, rga, rgl1, and rgl2) in the ga1-3 background allows to suggest the existence of DELLA-dependent and –independent gene expression pathways during germination (). NO is also necessary for the transcription of GA3ox1 and GA3ox2 genes, involved in the biosynthesis of GA, which is needed for the vacuolation of cells in isolated aleurone layers in the absence of NO (). This suggests the possibility that NO could coordinate the reduction of dormancy imposed by ABA with the initiation of germination stimulated by GA (Figure 1). Hydroxylamines, nitrites, and other nitrogenous compounds yielding NO under strong oxidation are the striking promotors for germination of some kinds of seeds ().
Root and Shoot Development
Plants have mechanisms for sensing nitrate as a signal for inorganic-N status, whereas metabolites derived from nitrate may serve as signals for organic-N status. It is also known than nitrogen sensing regulates phenotypic changes in plants. Nitrateactivated transcription of a set of genes is related to the positive regulation of nitrate transport, whereas ammonium or glutamine have been proposed to negatively regulate this process (). A root-specific ANR1 gene (a member of the MADS box transcription factors family), is nitrate-induced and appears to control lateral root growth (Zhang and Forde, 1998). There are examples of control by nitrogen both at the transcriptional and post-transcriptional level, and also examples of the matrix effect ().
In a nitrate re-addition experiment to Arabidopsis liquid cultures transcriptional reprogramming of hormone metabolism and sensing was identified as one of the early responses to nitrate addition (). Several genes involved in CK synthesis, including IPT3 and several downstream response elements involved in CK signaling, were induced around 30 min after adding 3 mM nitrate (KNO3), and other genes associated with CK signaling were up-regulated after 3 h. DF4, a gene involved in brassinosteroid biosynthesis and other genes involved in GA synthesis were also up-regulated after 3 h. Many 1-aminocyclopropane-1-carboxylate oxidases and other genes involved in ethylene synthesis and sensing were repressed.
ABA-insensitive mutants (abi4-1, abi4-2, and abi5) suffer a reduction of the systemic inhibitory effect of nitrate (). Among other features of their phenotype, they show an increased elongation of lateral root at high concentrations of nitrate, what suggests that ABA is an important mediator of the inhibitory effect of nitrate on lateral root elongation. In Arabidopsis, nitric oxide (NO) can reduce primary root growth and also induce lateral root development, both processes being also regulated by auxins. In A. thaliana there is also an anthocyanin biosynthesis pathway that is specifically induced by nitrate limitation, in which pathway NLA (Nitrogen Limitation Adaptation) is an essential molecular component ().
Auxins are essential for plant root development, and both shoot- and root-synthesized auxins contribute to root organization and development. Shoot-derived auxins move to root cells by diffusion, a mechanism that differs from the mechanism of directing auxin distribution through polar auxin transport (PAT) occurring in roots. The same pathway that is followed by carbohydrates from “source to sink” also enables the long-distance flow of auxin and other hormones such as ABA and cytokinins (). A good number of genes involved in auxin biosynthesis are expressed in roots, and the auxin that is generated in roots is partly responsible for the gradients that are necessary for normal root development (; ; ). Auxin signaling comprises a number of molecular components and works by stabilizing the interaction of the TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein or the AUXIN FBOX PROTEINs (AFBs) with proteins of the Aux/IAA family (; see Figure 2). TIR1 and AFB proteins contribute to substrate specificity for the E3 ubiquitin-ligase activity of Skp1-Cul1-F-box (SCF) complexes (). The IAA-stabilized interaction of SCF (TIR or AFB) enables Aux/IAA ubiquitination and the action of the 26S proteasome to remove these proteins ().
FIGURE 2
Nitric oxide treatment affects meristem size in the primary root mainly by decreasing cell-division rates and promoting cell differentiation (Figure 2; ). A reduction in meristem size was also observed in the NO overaccumulating mutant chlorophyll a/b binding protein underexpressed 1/NO overproducer 1 (cue1/nox1). Interestingly, the root apical auxin maximum is altered after NO addition. Auxin transport and the level of the auxin efflux protein PINFORMED 1 (PIN1) are reduced significantly in the cue1/nox1 double mutant. The abnormal organization of the quiescent center and surrounding cells of cue1/nox1 double mutants resembles the phenotype of pin1-mutant roots, what suggests a connection between NO and auxin signaling in maintaining the activity and the size of the root apical meristem ().
Changes in NR levels during plant growth modify NO levels and would link metabolism to gene regulation, and NO derived from NR activity modulates group VII ERF transcriptional regulators stability through targeted proteolysis (N-End Rule Pathway in plants; ). Still it is necessary to check if, similarly to seed germination, group VII ERFs regulate NO/ABA signaling by controlling the expression of ABI5 (ABA INSENSITIVE 5) in seedlings grown under different C/N regimes.
Nitrogen nutrition has significant effects on root and shoot relations (; ). Nitrogen deficiency increased root surface area, increased consumption of assimilates, reduced the amount of nitrogen translocated to shoot, diminished shoot growth, and yielded an increased root/shoot ratio. Extra nitrogen nutrition, however, caused a shoot overgrowth, reduced the amount of assimilates availability for root, and decreased the root/shoot ratio ().
All in all, root growth regulation could be summarized as follows: auxin flows from the lateral root cap to the basal meristem and returns to the root tip (). Nitrate is reduced to nitrite by nitrate reductase in the root. NIA/NR- and AtNOA1 (Nitric Oxide-Associated1)-mediated synthesis of NO is active and generates most of the NO in Arabidopsis (). NO maintains auxin levels by inhibiting IAA oxidase activity (), but high NO interfere with acropetal auxin transport through PIN1 auxin efflux carrier, which correlates well with a reduced response to auxin (; ). NO also has a positive effect on auxin signaling through S-nitrosylation of TIR1 (). In N-replete seedlings (lower C/N ratio condition), increased NO levels would nitrosylate ABI5 as a regulator to control ABA hormone signaling through its degradation (). On the other hand, ABI5 is stabilized through its phosphorylation upon ABA treatment becoming active (). Therefore, characterization of ABI5 post-translational modifications is relevant, and it bound to NO interference on acropetal auxin transport through PIN1 auxin efflux carrier, establishes a limited root development (Figure 2A). Under low nitrate conditions, because of reduced NO levels, the auxin that is generated in the root contributes to maintain the gradients and maxima required for enhanced root development (; ; ). Additionally, in N-starved seedlings, auxin and cytokinins (CKs) could increase NO production to basal levels, similar to other stresses such as iron deficiency (). CKs are known to induce NO biosynthesis depending on plant cell status (), and auxins could increase NO production similarly to other stresses such as iron deficiency (). These minimum NO levels would not be enough to repress PIN1 expression, not altering acropetal auxin transport, therefore promoting enhanced root growth (Figure 2B). It seems that low NO levels generated in N-starved root seedlings (higher C/N ratio) are determinants for enhanced lateral root growth rate (Figure 2B). Moreover, ABI5 post-translational state could regulate their downstream transcription factors in an exquisite way, causing an effect in metabolism, growth and plant development.
C-Control of Development
Seed Dormancy and Germination
In Arabidopsis seeds, dormancy release can be activated by cold, nitrate and light. The efficiency of this activation depends on the extension of the dry after-ripening period following harvest (). Light is essential for germination, though dormancy is not released by light if seeds have not been subject to an extended period of after-ripening or a combination of a shorter period of after-ripening plus imbibition on a nitrate solution or cold treatment.
Regulation of seed germination under an excess of nutrient supply has also been deeply studied. CHO1 codes for a putative transcription factor with two AP2 domains, expressed predominantly in seed, with the strongest expression 24 h after seed imbibition. Under an excess of glucose an nitrate supply, ABA signaling pathway could be influenced at different levels, and transcription factor CHOTTO1 (a double APETALA2 domain protein of Arabidopsis thaliana) is responsible for germination arrest (). Arabidopsis cho1 mutants are resistant to the ABA analog (–)-R-ABA during seed germination and seedling development (). Only weak resistance to exogenously applied (+)-S-ABA was observed during seed germination of cho1 seeds, and abi4 cho1 double mutant seeds germinated at rates comparable to those of the abi4-5 mutant seeds in the presence of exogenous (+)-S-ABA. Moreover, the induction of CHO1 expression did not occur in the abi4 mutants, suggesting that CHO1 acts in the same pathway as ABI4. ABI4 is involved in glucose signaling, and therefore operates the downstream target CHO1. As a result, both abi4 and cho1 mutants exhibit growth resistance to high concentrations of glucose. In contrast, CHO1, but not ABI4, is required for inhibition by excess of nitrate (Figure 1). The inhibitory action of different sugars on seed germination could proceed through different pathways, including complex interactions with those on phytohormone-response. A high level of glucose is responsible for the induction of ABA synthesis and also induces the expression of ABI4 and ABI5. This suggests that at least some aspects of sugar signaling may be mediated by ABA response (). Furthermore, glucose hypersensitivity results by overexpressing ABI3, ABI4, or ABI5, what suggests that these ABI genes could mediate the response to ABA and sugar (); in fact, the induction of these ABI genes is stronger when glucose is added to the medium during early stages of seedling development, right when the plant is most sensitive to the inhibitory effects of sugar () and ABA induces ABI5 accumulation (). In Lotus japonicus, at concentrations up to 200 μM, nitrite and nitrate alleviated glucose-induced delay of seed germination, in a NO mediated response (Zhao et al., 2008).
Several sugars may delay seed germination via different pathways. showed that glucose modulates a decreased in ABA concentration during germination in wildtype Arabidopsis seeds (Figure 1). Seed germination of Lotus japonicus was reported to be delayed by exogenous glucose (Zhao et al., 2008) and there was a significant inhibitory effect on seed germination with 2.5% glucose after 72 h. Inhibition of seed germination by glucose was substantially alleviated by exogenous supply of SNP (sodium nitroprusside), an exogenous donor of NO, (2.5% glucose + 200 μM SNP), and this effect was significantly reduced by cPTIO [carboxy-PTIO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide], a NO scavenger (200 μM), suggesting that the effect of SNP is likely due to NO. Germination of Lotus japonicus seeds was insensitive to KNO2 and KNO3 at concentrations up to 200 μM in the absence of glucose. At these concentrations, nitrite and nitrate alleviated glucose-induced delay of seed germination.
Auxins have been involved in cell-wall remodeling (), and imbibed after-ripened seeds, showed an enhanced expression of genes related to RNA translation, protein degradation and cell wall modification than dormant seeds (), and the expression of these genes increases within 1 and 3 h in imbibed after-ripened seeds (). Genes involved in auxin transport (AUX1, PIN1, PIN7) are highly upregulated following imbibition of after-ripened seeds (; ) or addition of gibberellin (GA; ), suggesting the initiation of root growth at the onset of phase III.
In short, seed germination is controlled by nutrient status through multiple action points. This implies a bona fide perception of nutrient balance, acting through signaling pathways either repressing or promoting seed germination.
Root and Shoot Development
C-status ultimately controls many aspects of plant development. Vegetative growth is controlled by the cellular metabolic status (). Sugars are long-distance signals and there are sugar-dependent regulatory networks in roots. SnRK1 is a growth inhibitor under nutrient stress conditions (). Sugar-phosphates regulate plant SnRK1 (). Glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), and trehalose-6-phosphate (T6P) are repressors of SnRK1 activity (Zhang et al., 2009). Sucrose promotes the accumulation of T6P by inhibiting SnRK1 activity, thereby inducing biosynthetic processes and plant growth. Plant SnRK1 controls several important enzymes, such as nitrate reductase and sucrose phosphate synthase (). This suggests that SnRK1 could play a relevant role in controlling C/N metabolism (). The ABA effect could be related to SnRK1, since phosphatases implicated in ABA signaling seem to inhibit SnRK1 activity through SnRK1 dephosphorylation (). Arabidopsis group C/S1 basic leucine zipper (bZIP) consists of nine bZIP transcription factors network and depends on the presence of bZIP proteins in a particular cellular context. The C/S1 network of bZIP factors works efficiently to integrate signals (). All these bZIP factors are expressed specifically in sink organs like young leaves, anthers and seeds. This fact suggests that the C/S1 network is involved in nutrients allocation to sink organs (,). Sucrose inhibits the translation of S1-group bZIP mRNAs. The SnRK1 pathway has been assigned the function of adjusting the growth and development of the plant to its energy status (). The SnRK1 pathway increases the transcriptional potential of S1 class bZIP proteins () and SnRK1 seems to be implicated in the down-regulation of ribosomal protein (). In a high metabolic status, T6p inhibits SnRK1, and the active TOR kinase stimulates translation and growth, being important for S1-group bZIP protein synthesis capacity via effects on ribosome biogenesis, mRNA polysome loading and mRNA translation (; ).
Sucrose-induced repression of translation (SIRT) of the S1-group bZIP transcription factors has been confirmed for all five Arabidopsis S1-group members (; ; ). Sucrose stimulates the activation of the AKIN complex in Arabidopsis () and plant SNF1 may mediate the activation of gene expression by sucrose and glucose (; ). PRL1 is a potential subunit of the Arabidopsis AKIN complex and is a putative negative regulator of AKIN, yet the mutant is hypersensitive toward glucose (). Two Arabidopsis protein kinases, KIN10 and KIN11, seem to be involved in the control of transcription convergent reprogramming as a response to darkness, sugar and stress conditions, three apparently unrelated factors; in addition, specific bZIP transcription factors partially mediate primary KIN10 signaling. KIN10 targets promote catabolism and suppress anabolism by sensing and signaling sugar and energy deprivation ().
Hexokinases play an important role in a hexokinase-dependent sugar response pathway (). Several glucose signal transduction pathways are known: a first one is AtHXK1-dependent, a second one is glycolysis-dependent and influenced by AtHXK1 activity, and a third pathway that is AtHXK1-independent (). The hexokinase-signaling pathway might play a role in cell-cycle control linked to the carbohydrate status, and sugar regulation of cycD2 seems to be mediated by hexokinase (). Arabidopsis HXK1 plays a relevant role in a number of glucose responses, namely cell proliferation, root and inflorescence growth, leaf expansion and senescence, and reproduction. HXK1 can play different functions in glucose signaling and metabolism; in fact, many of these new HXK1 functions can be performed at least partially by catalytically inactive HXK1 mutants (). Growth promotion or inhibition by HXK1 would depend on glucose concentration, cell type, developmental state, and environmental condition (). In the absence of nitrate, low glucose level signaling is HXK1 mediated but is independent of ABA and ethylene signaling. At low glucose levels, ABI4 and ABI5 gene expression is HXK1 dependent, while at high glucose levels these genes are expressed independently of HXK1 ().
Sucrose effects on auxin levels are more pronounced in roots than in shoots, suggesting that sugars may impact auxin transport and/or conjugation pathways as well (). Auxins and CKs present links to sucrose sensing and signaling, and can function as short- and long-distance signaling molecules. They can play a role in integration of growth and development between shoot and root (). The rapid response around 30 min after adding 15 mM sucrose to C-starved seedlings, of an Aux/IAA family member IAA5 () is indicative of early crosstalk with auxins (). Auxin biosynthesis is induced by soluble sugars, and daily fluctuations in sugar content are correlated with fluctuations in auxin levels (). In Arabidopsis, exogenous sucrose or glucose supply triggers an over-accumulation of auxin and increases the auxin flux in the hypocotyl notably through the up-regulation of the genes encoding auxin biosynthetic enzymes, including CYP79B2/3 and YUCCA8/9 (; ). Sucrose supplementation, required for rhythmic hypocotyl elongation, induces YUCCA9 in shoots but not roots and sucrose effects on auxin levels are more pronounced in roots than in shoots, suggesting that sugars may impact auxin transport and/or conjugation pathways as well (). Auxins and sugars can be transported from shoot to root, inducing lateral root development in order to increase water and nutrients uptake from the soil, in turn increasing shoot growth capacity ().
CKs regulate the expression of sucrose transporters involved in phloem unloading adjusting sugar partitioning and sink strength, and they also regulate invertases and hexose transporters involved in sucrose catabolism and uptake in sink tissues (; ).
Sugars like trehalose 6-phosphate (Tre6P) is both a signal of sucrose status and a negative feedback regulator of sucrose levels (; Figure 3). Tre6P can affect developmental processes such as shoot branching. The impact of sugar during the systemic regulation of bud outgrowth in response to either decapitation or light intensity has been analyzed by . Axillary buds outgrowth is driven by sugar availability independently of auxin levels. The combination of high sugar levels in shoot and high sugar sink strength in buds (high photosynthesis rate) drives to high branching. Sugars play a signaling role, and not only a trophic role ().
FIGURE 3
Slower growth at night in the starchless mutant, which shows higher sucrose levels during the day and absence of sugars at the end of the night (), requires an adjustment of GAs level to match the lower growth potential deriving from the lack of sugars at night (). The dwarfism of GA-deficient mutants is, instead, uncoupled from carbon availability (), indicating that GA is primarily required for growth. DELLA proteins seem to represent a point of convergence for the hormonal and sucrose-dependent regulatory networks and DELLA are involved in the sucrose-GA interaction (; Figure 3). Sucrose-dependent stabilization of DELLAs () seems to be a simple mechanism to connect sugars with other signaling pathways (). Cell expansion in the elongation zone of growing roots is controlled by GA, and the endodermis plays a key role in this process. Growth of these root tissues is regulated by endodermis-specific removal of DELLA repressor proteins mediated by GA, ensuring that cells from all elongation zones respond similarly to GAs expanding at the same rate ().
The Phytochrome-Interacting Factor (PIF) family of transcription factors seem to have their basic helix-loophelix in every process involving light, temperature and growth (). DELLA and PIF proteins are good candidates for molecular hubs operating at the crossroads of many pathways (; Figure 3). PIFs attenuate the light signal through negative feedback on phytochrome transcription, as well as by bringing them along when they are targeted for proteasome-mediated degradation (). A combination of circadian clock and light regulation control the activity of PIF4 and PIF5, leading to predictable daily oscillations in seedling growth rates (). These PIF-driven growth cycles depend on supplying seedlings with exogenous sucrose (; ; Figure 3).
Nitric oxide regulates GA signaling by controlling DELLA abundance and function. suggested that NO could induce the accumulation of DELLAs, despite finding no transcriptional induction of DELLA genes. There are NO and GA antagonist functions in the control of light-regulated photomorphogenesis through the balance between DELLAs and PIFs (; Figure 3).
A sugar-sensing pathway has been discovered in cultured Arabidopsis cells: protein phosphorylation, 14-3-3 binding, and regulatory proteolysis control diverse target proteins in C/N metabolism and signaling. Several cytosolic enzymes involved in C/N metabolism (nitrate reductase, glutamine synthetase, sucrose-phosphate synthase, trehalose-6-phosphate synthase and glutamyl tRNA synthetase) are relevant targets of this pathway (; ). We must consider the matrix effect, since phosphorylation and 14-3-3 binding of distinct subsets of these enzymes seem to be regulated by photosynthesis and hormones (). When cells are starved of metabolizable sugars, 14-3-3-binding is lost and its targets are clipped by a specific cysteine protease.
Further studies are required to elucidate the signals responsible for nutrient signaling pathways in the regulation of root and shoot growth. CKs, auxins, GAs and sugars function as long-distance signals and a low level of sugar in roots implies that GAs are transported from the roots to the shoots. Sucrose availability, which implies a high metabolic status, is correlated with plant T6P levels and T6P inhibits SnRK1. It is known that metabolic events associated with a high concentration of carbohydrates could be crucial rather than the high concentrations of carbohydrate (). Additionally, the balance between DELLAs and PIFs is suggested to control light-regulated photomorphogenesis ().
C and N Interaction Pathways (C/N Balance)
C/N signaling systems are influenced by the biological context such as cell-type, developmental, metabolic, and/or environmental conditions, as already suggested by .
As previously mentioned, only a few genes involved in C/N balance response have been described. NRT2.1 and NRT2.2 are the main highaffinity nitrate transporters in roots under N-replete conditions (). In their absence, an additional loss of function of NRT2.4 has a significant impact on plant fresh weight in N-starved plants suggesting a complex interaction between them in response to changes in N availability (). NRT2.1 is also known to play a role in the repression of lateral root initiation under high sucrose/low nitrate context, acting as either a sensor or a signal transduction protein in low nitrate conditions (). However, additional studies are required in order to elucidate how NRT2.1 regulates pericycle cell divisions in function of C/N external balance.
GLR1.1 (putative glutamate receptor 1.1) functions as a molecular regulator in the high C/low N response and its consequent implication in ABA metabolism and sensitivity, and response to water stress in Arabidopsis. AtGLR1.1 is a component of the HXK1-independent ABA pathway in germinating seeds and functions as a sensor pointing its true ligand to the Glu. GLR1.1 can be expressed in the presence of a N source such as nitrate or amino acids (Glu or Gln), promoting seed germination (; ).
OSU1/QUA2/TSD2 encodes a putative methyltransferase which acts in cell wall biogenesis () and is required for normal C/N nutrient balance response in plants (). OSU1/QUA2/TSD2 might have different targets which in turn regulate pectin biosynthesis and C/N balance responses, respectively. This represents a surprising link between cell wall biogenesis and C/N balance response (Zheng, 2009). OSU1 is likely to act as a negative modulator either in distinct pathways leading to high C/N ratio or low C/N ratio responses or in a single pathway regulating various transcription factors in response to these distinct C/N imbalance conditions (Zheng, 2009).
Ubiquitin ligases are known to play an essential role in C/N balance, mediating nutrient-responsive pathways. KEEP ON GOING (KEG), a RING-type ubiquitin ligase in Arabidopsis, localizes to trans-Golgi network/early endosome (TGN/EE) vesicles and adjusts ABI5 levels by poly-ubiquitination in function of C and N metabolite availability (). It has been proposed that KEG recognizes ABI5 through one or more of its ANK (Ankyrin) repeats () and its interaction with EDR1 is mediated by the HERC2-like repeats (). KEG plays a relevant role in promoting post-germinative growth (). Pointing in this direction is ATL31, a RING-H2-type ubiquitin ligase, which plays a key role in regulating the response to C/N conditions during post-germinative growth in Arabidopsis. Under low C/N conditions, ATL31 target protein degradation through 26s proteasome pathway entails post-germinative growth. The ATL31 protein localizes to the membrane and recruits target proteins for ubiquitination and degradation by the 26S proteasome, allowing seedling development proceed through the early post-germinative growth arrest checkpoint (). Protein kinases catalyze ATL31 phosphorylation, which binds to 14-3-3 proteins and mediates their stability. In low C/high N conditions, the protein kinases catalyzing ATL31 phosphorylation are mostly inactivated. In high C/low N conditions, these protein kinases are active and catalyze the phosphorylation of 14-3-3 binding sites on ATL31, which binds 14-3-3 proteins and consequently perform their ubiquitination and proteasomal degradation (). Studies carried out by demonstrated that Arabidopsis plants are equipped with a molecular mechanism to adapt to nitrate limitation in which NLA (Nitrogen Limitation Adaptation), a putative RING-type ubiquitin ligase, is involved in the ubiquitination-mediated degradation or modification of substrate protein(s) and function as a positive regulator for the adaptation response to nitrogen limitation.
There are recent experiments pointing out the involvement of specific ABA and SnRK1s signaling pathways in C/N response under ABI1 regulation. In high C/low N condition, identified genes whose expression is upregulated as RD29b, LEA3-4, and TSPO and suppressed in ABI1 over-expressing plants, whereas other genes as RAB18, AREB1, and ABF3, were not affected. Additionally, SNRK1s-responsive genes as DIN6 and SEN5 were downregulated in wild-type plants and this effect was suppressed in ABI1 over-expressing plants. SnRK1s proteins are known to phosphorylate several 14-3-3 targeting proteins, which play a role in C and N metabolism, such as nitrate reductase (NR) and sucrose 6-phosphate synthase (SPS; ).
Carbon- and nitrogen-signaling pathways interact according to the external C/N status and plants have a C/N sensing and regulatory mechanism. Genome-wide analysis due to C and N signaling interactions in Arabidopsis revealed that C is a more ubiquitous regulator of the genome than N (). These authors identified 3 MIPS FunCats corresponding to metabolism, protein synthesis and energy genes that are regulated by C/N signaling. The accurate regulation of metabolic genes is essential to cope with C/N status. The identification of putative cis-acting regulatory elements involved in C and N signaling interactions indicates the existence of multiple mechanisms for C/N status response. Two transcriptional mechanisms are suggested: (i) independent regulation of C-element and C-Nelement and (ii) dependent regulation of N-dependent enhancer of C regulation on a C-responsive transcription factor and cis element ().
Taken together, a complex picture emerges in which nitrate transporters, glutamate receptors, methyltransferases and ubiquitin ligases are acting on multiple levels to integrate C and N signaling interaction pathways and regulate energy and metabolic genes as well as protein expression. An exquisite control comprises of several transcriptional mechanisms assuring an accurate response for the C/N status.
Concluding Remarks
In this review, we have focused on how changes in C and N levels regulate the production of NO, which acts in plant developmental processes through the interaction with phytohormones and other plant growth regulators, using similar molecular elements. Recent investigations have the goal to shed light on the molecular mechanisms underlying the crosstalk of nutrients with NO and ABA response. This ambitious goal includes several tasks such as analyzing the regulation of NO and ABA production by changes in the C/N status, the characterization of mutants impaired in either NO or nutrients response (cue1, Atnoa1-2, nia1nia2, gin5, gin6…), the participation of nutrients in the regulation of lateral root growth by NO and, finally, the functional characterization of transcription factors responsive to nutrients, NO and ABA, unraveling their implication in different nutrients signaling pathways. NO is key to control many of the developmental outcomes in response to N, C and N/C. NO interference on acropetal auxin transport controls root development () and NO nitrosylates ABI5 as a regulator to control ABA hormone signaling through its degradation (). The implementation of the “omics” sciences will help to get insight into the nutrients and hormones crosstalk signaling pathways.
It is important to underline that our current understanding of C and N-dependent signaling pathways in seeds germination and plant development is mainly related to the model plant Arabidopsis. Therefore, it is necessary to elucidate nutrientsensing and signaling pathways in other plants.
As a useful application of the knowledge of these molecular mechanisms, development of perennial versions of important grain crops is crucial for the increasing worldwide food demand. Root branching order is the main determinant of root trait variation among species () and nutrients can influence root structure. Perennial crops generally have advantages over annuals in maintaining important ecosystem functions, particularly on marginal landscapes or where resources are limited (). Therefore, identification and molecular characterization of genes involved in nutrients crosstalk with root development signaling pathways could be the basis for the generation of perennial crops through crop breeding.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Statements
Acknowledgments
This research was supported by grant AGL2012-33268 from the Spanish Ministerio de Economía y Competitividad from the FP7 and The European Regional Development Fund (FEDER) from the European Union.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
carbon, nitrogen, nitric oxide, abscisic acid, auxin, gibberellin, cytokinin
Citation
Osuna D, Prieto P and Aguilar M (2015) Control of Seed Germination and Plant Development by Carbon and Nitrogen Availability. Front. Plant Sci. 6:1023. doi: 10.3389/fpls.2015.01023
Received
19 August 2015
Accepted
05 November 2015
Published
18 November 2015
Volume
6 - 2015
Edited by
Polydefkis Hatzopoulos, Agricultural University of Athens, Greece
Reviewed by
Elena A. Vidal, Pontificia Universidad Catolica de Chile, Chile; Hao Peng, Washington State University, USA
Updates
Copyright
© 2015 Osuna, Prieto and Aguilar.
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*Correspondence: Daniel Osuna, dosuna@ias.csic.es
This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science.
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