Tissue- and Cell-Specific Cytokinin Activity in Populus × canescens Monitored by ARR5::GUS Reporter Lines in Summer and Winter

Abstract

Cytokinins play an important role in vascular development. But knowledge on the cellular localization of this growth hormone in the stem and other organs of woody plants is lacking. The main focus of this study was to investigate the occurrence and cellular localization of active cytokinins in leaves, roots, and along the stem of Populus × canescens and to find out how the pattern is changed between summer and winter. An ARR5::GUS reporter construct was used to monitor distribution of active cytokinins in different tissues of transgenic poplar lines. Three transgenic lines tested under outdoor conditions showed no influence of ARR5::GUS reporter construct on the growth performance compared with the wild-type, but one line lost the reporter activity. ARR5::GUS activity indicated changes in the tissue- and cell type-specific pattern of cytokinin activity during dormancy compared with the growth phase. ARR5::GUS activity, which was present in the root tips in the growing season, disappeared in winter. In the stem apex ground tissue, ARR5::GUS activity was higher in winter than in summer. Immature leaves from tissue-culture grown plants showed inducible ARR5::GUS activity. Leaf primordia in summer showed ARR5::GUS activity, but not the expanded leaves of outdoor plants or leaf primordia in winter. In stem cross sections, the most prominent ARR5::GUS activity was detected in the cortex region and in the rays of bark in summer and in winter. In the cambial zone the ARR5::GUS activity was more pronounced in the dormant than in growth phase. The pith and the ray cells adjacent to the vessels also displayed ARR5::GUS activity. In silico analyses of the tissue-specific expression patterns of the whole PtRR type-A family of poplar showed that PtRR10, the closest ortholog to the Arabidopsis ARR5 gene, was usually the most highly expressed gene in all tissues. In conclusion, gene expression and tissue-localization indicate high activity of cytokinins not only in summer, but also in winter. The presence of the signal in meristematic tissues supports their role in meristem maintenance. The reporter lines will be useful to study the involvement of cytokinins in acclimation of poplar growth to stress.

Introduction

Cytokinins are adenine derivatives that act as master regulators of plant growth and development. They are synthesized mainly in the root tips (Dieleman et al., 1997; Miyawaki et al., 2004; Aloni et al., 2005), but also locally in shoot tissues (Sakakibara, 2006; Tanaka et al., 2006; Hirose et al., 2008; Kamada-Nobusada and Sakakibara, 2009). Root-derived cytokinins are transported acropetally through xylem sap by the transpirational pull (Aloni et al., 2005), while shoot-derived cytokinins are transported through phloem (Bishopp et al., 2011). Active and inactive forms of cytokinins occur as free bases and as ribosides, ribotides, or glucose conjugates, respectively (Mok and Mok, 2001; Romanov et al., 2006).

Cytokinins have roles in almost all aspects of plant growth and development including cell division, shoot initiation and growth, sink/source relationships, nutrient uptake, breaking of bud dormancy, delay of leaf senescence, and regulation of vascular development (Hwang et al., 2012; Kieber and Schaller, 2014). Cytokinins determine vascular cell identities, except those of the protoxylem (Mähönen et al., 2000; Hutchison et al., 2006; Yokoyama et al., 2007; Argyros et al., 2008) and promote the development of vascular cambium (Matsumoto-Kitano et al., 2008; Nieminen et al., 2008). Cytokinins specify the vascular pattern by regulating the level of PIN auxin efflux proteins (Bishopp et al., 2011). Cytokinins increase the sensitivity of the cambium to the auxin signal thereby determining wood quantity and quality (Aloni, 1991, 2001).

Cytokinin perception and signaling in plants has been extensively studied in Arabidopsis and involves a His-Asp phosphorelay that mediates the signal transmission (Mizuno, 2005; Schaller et al., 2008). Among the response regulators in this pathway, type-A ARRs (Arabidopsis Response Regulators), i.e., genes which contain the highly conserved Lys and two Asp residues in their receiver domains, are the primary response genes for cytokinins (D’Agostino et al., 2000). Ten type-A ARR genes are described in Arabidopsis (D’Agostino et al., 2000; Schaller et al., 2008; Pils and Heyl, 2009) and eleven in Populus trichocarpa (Ramírez-Carvajal et al., 2008; Immanen et al., 2013). The ARR genes are transcriptionally regulated and can be induced by exogenous cytokinin treatment (D’Agostino et al., 2000; Taniguchi et al., 1998).

In trees, changes in endogenous cytokinin levels in relation to seasonality have been studied for a long time. Most of these studies focused on the endogenous cytokinin levels in xylem or phloem sap of the trees (Hewett and Wareing, 1973; Alvim et al., 1976; Weiler and Ziegler, 1981; Tromp and Ovaa, 1990; Cook et al., 2001) or reported the endogenous cytokinin concentrations in different organs (Hewett and Wareing, 1973; Van Staden and Dimalla, 1981; Cook et al., 2001). Furthermore active and inactive forms of cytokinins were distinguished (Hewett and Wareing, 1973; Van Staden and Dimalla, 1981; Tromp and Ovaa, 1990) and their changes were related to seasonal fluctuations (Tromp and Ovaa, 1990). For example, in the xylem sap of apple trees, the active trans-zeatin type (tZ) levels were high during the growing season, dropped during dormancy and showed an increase during bud burst, whereas continued to increase during the growing season (Tromp and Ovaa, 1990). Despite the importance of cytokinins in vascular development, knowledge on the cellular localization of this growth hormone in the stem and other organs of woody plants is still lacking. Furthermore, it is unclear how the tissue-specific distribution of active cytokinins is influenced by dormancy.

The goal of this study was to investigate the occurrence and cellular localization of active cytokinins in leaves, roots and along the stem of poplar and to find out how the pattern is changed between the active growth phase in summer and dormancy in winter. Our hypothesis was that cytokinin activity was present in actively growing tissues in summer and lacking in winter, except in those tissues, where cells have to be kept in the meristematic stage. Tissue-specific localization patterns of cytokinin activity were also compared with expression of genes belonging to the type-A Response Regulator (RR) family in poplar. In Arabidopsis, the ARR5::GUS (β-glucuronidase) reporter construct has been used to monitor the distribution of active cytokinins in different tissues (D’Agostino et al., 2000). ARR5 has high homology to the cytokinin-inducible gene PtRR10 of Populus trichocarpa (Ramírez-Carvajal et al., 2008; Immanen et al., 2013). Here, we employed the ARR5::GUS construct as a tool to investigate the localization pattern of active cytokinins in poplar. The transgenic poplar cytokinin reporter lines were grown outdoors under ambient conditions and used to map ARR5 activity in summer and winter.

Materials and Methods

Plant Transformation

The ARR5::GUS construct described in D’Agostino et al. (2000) was provided by Prof. Kieber (University of North Carolina, Chapel Hill, NC, USA), cloned, transformed into Agrobacterium tumefaciens strain C58C1/MP90 and then used to transform Populus × canescens [INRA (Institut National de la Recherche Agronomique) clone 717-1B4] as described by Teichmann et al. (2008). Plantlets were regenerated, maintained on Murashige and Skoog (MS) medium containing 50 mg l^-1 kanamycin and micropropagated after Leple et al. (1992).

Selection of Transgenic Reporter Lines

Leaves from 3-week-old transformed plantlets were collected and GUS staining was performed according to Jefferson et al. (1987) as modified by Teichmann et al. (2008). Briefly, the presence of GUS activity was investigated in intact leaves that were vacuum-infiltrated with GUS buffer (100 mM NaH₂PO₄, pH 7.0, 10 mM Na₄EDTA, 0.05% Triton X-100) containing 1 mg ml^-1 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid (Duchefa, Haarlem, The Netherlands). The leaves were then incubated in the dark at 37 °C for 24 h and chlorophyll was removed by ethanol treatment. The ARR5::GUS activity was observed mainly in the petiole and primary veins of these leaves. The leaves were viewed and photographed directly. From the regenerated plantlets which were maintained on MS medium containing kanamycin, 17 lines showed GUS activity after GUS staining, mainly in the veins (Figures 1A,B). The pattern was similar to that of mock treated leaves (Figures 1C,D).

FIGURE 1

All plantlets from these 17 lines showed similar morphology and growth in vitro when compared to the wild-type (WT). Plantlets from each line were propagated in vitro. The leaves from these lines were also treated with an exogenous cytokinin supply in order to monitor the response of ARR5::GUS construct to cytokinin. For this purpose, leaves from 3-week-old in vitro micropropagated plantlets were fed by the petioles with a solution containing 5 μM thidiazuron (Duchefa, Haarlem, The Netherlands) or 5 μM 6-benzylaminopurine (BAP, Sigma–Aldrich Chemie, Steinheim, Germany) in 0.1% DMSO (Merck KGaA, Darmstadt, Germany), both active cytokinin analogs or 5 μM adenine (Sigma–Aldrich Chemie, Steinheim, Germany), which is an inactive cytokinin analog. A solution of 0.1 % DMSO (Merck KGaA, Darmstadt, Germany) was used as the solvent control. During the treatment the leaves were kept inside a sealed glass jar at high humidity to avoid desiccation stress. The leaves were allowed to transpire under 200 μmol quanta m^-2 s^-1 photosynthetically active radiation (PAR) for 24 h. Afterward, the leaves were directly used for GUS staining as above. Examples for cytokinin induction of the ARR5 promoter in thidiazuron- or BAP-fed leaves are shown in Figures 1E,F. When compared to controls, the thidiazuron-treated leaves showed a strong induction in ARR5::GUS activity over the leaf blade and in tertiary veins (Figure 1E). On the other hand, the leaves treated with the inactive cytokinin analog adenine did not show an induction (Figures 1G,H). For documentation of staining pattern, the leaves were laid flat in a petri dish filled with distilled water and were scanned (Canoscan 4400F, Canon Inc., China).

From the lines that showed an increased ARR5::GUS activity under exogenous cytokinin treatment, three lines (line 9, 32, and 80) were selected for the study.

Plant Cultivation

In vitro micropropagated plantlets of the lines 9, 32, and 80 along with WT, were grown in hydroponics for 3 weeks and then transferred into pots with soil (Fruhstorfer Erde Type N, Hawite Gruppe GmbH, Vechta, Germany) as described by Müller et al. (2013). The plants were grown in a greenhouse for 3 months under controlled environmental conditions: 16 h day length, 200 μmol quanta m^-2 s^-1 PAR, 20 °C air temperature and 55% relative air humidity. Afterward, the potted plants were transferred from the greenhouse to a caged area outdoors (Göttingen, Germany, 51.55739°N, 9.95857°E, 293 m above sea level) and acclimated to ambient light and temperature (after Müller et al., 2013). On 18th July 2011, the poplars were planted in four boxes (3.5 m length × 3 m width × 0.7 m height) filled with a compost soil and sand mixture (1:1) (Vogteier Erdenwerk GmbH, Niederdorla, Germany). The WT and transgenic lines were planted in a mixed design. Each box was equipped with a total of 42 plants comprising 10 plants each of WT, line 9 and 80, and 12 plants of line 32. The first row of plants near to the box edges was not included in any of the analyses to avoid edge effects.

During the growing season, plants were watered with tap water every second day or daily on warm days. Air temperature, relative humidity, and PAR for every hour per day were recorded during the whole study period using MeteoLOG TDL 14 data logger (Adolf Thies GmbH & Co. KG, Göttingen, Germany).

Harvest

Harvests were conducted in the growing season (August, 2012) when the mean temperature was 22.4 °C and during dormancy (January, 2013) when the mean temperature was -5.0 °C. In the growing season harvest, four plants each from WT, line 9, 32, and 80 were harvested. In the dormancy harvest, one plant from WT and two plants from each line 9, 32, and 80 were harvested. Roots, bark, wood, and leaves were separated and fresh mass was determined for each fraction. Aliquots of these plant tissues were oven dried at 60 °C for 7 days for measurement of dry mass. Tissue dry mass (g) was calculated as:

During harvest, the following fresh tissues were collected for GUS staining: one half of the apical bud, leaf disks (diameter 5 mm) from the first fully developed leaf from the apex, stem cross sections (2 mm thickness) at three positions: top (50 mm beneath the stem apex), middle (the position in the stem exactly in the center between the apex and the shoot–root junction) and bottom (50 mm above the root–shoot junction), and fine root tips. The stem cross sections were cut using a micro-saw (Proxxon, Föhren, Germany). The materials were directly transferred into the GUS buffer and GUS staining was performed as described above.

GUS Activity Analyses at Tissue and Cellular Level

Tissue staining patterns were documented by photos, which were taken with a digital camera (DFC420 C, Leica Microsystems Ltd., Germany) attached to a stereomicroscope (M205 FA, Leica Microsystems Ltd., Wetzlar, Germany). The stained tissues were fixed in a solution of 1 part of 37% formaldehyde, 1 part of 100% acetic acid, and 18 parts of 70% ethanol. Subsequently, the fixed tissues were dehydrated in a series of ethanol solutions (70, 80, 90, and 96% (v/v)) for 2 h each at room temperature. The tissues were embedded in Technovit 7100 resin (Heraeus Kulzer GmbH & Co. KG, Germany) according to manufacturer’s instructions with the following modifications: The dehydrated samples were infiltrated in 1:1 (v/v) solutions of 96% ethanol and Technovit 7100 basic solution for 5 h. Then the samples were infiltrated in 1:2 and then in 1:3 (v/v) solutions of 96% ethanol and Technovit 7100 basic solution for 12 and 5 h, respectively. Thereafter, the samples were treated with Technovit 7100 infiltration medium consisting of 1 g Hardner I in 100 ml Technovit 7100 basic solution (provided by the manufacturer) for 24 h. A reduced pressure of 20 kPa for 15 min was applied at each step during infiltration. Finally, the tissues were embedded in the embedding medium (prepared by mixing 30 ml infiltration medium and 1.5 ml Hardner II provided by the manufacturer). Sections of 15 μm thickness were cut with a rotarymicrotome (RM 2265, Leica Microsystems, Wetzlar, Germany) and viewed under a microscope (Axioplan Observer.Z1, Carl Zeiss GmbH, Germany). Photographs were taken at 100× and 200× magnification with a digital camera (Axio Cam MRC, Carl Zeiss Microimaging GmbH, Göttingen, Germany) attached to the microscope (Axioplan Observer.Z1, Carl Zeiss GmbH, Germany).

Gene Expression Analyses of PtRR Type-A Family

For the analysis of tissue specific expression patterns of poplar genes belonging to the two component RR type-A gene family of cytokinin signaling pathway, the gene list as reported by Ramírez-Carvajal et al. (2008) was used. The genomic sequence of each gene was obtained from Joint Genome Institute, JGI¹ and the respective gene IDs were obtained by blasting the genomic sequences in Phytozome v10.1² (Goodstein et al., 2012). The homolog of each gene in Arabidopsis was obtained by blasting the protein sequence taken from Phytozome, in TAIR³. The gene names and gene IDs of the genes used for the expression analyses have been compiled in Table 1. Microarrays were downloaded from the EMBL-EBI ArrayExpress database (Kolesnikov et al., 2015). For P. × canescens: E-GEOD-16495 (shoot apex; Yordanov et al., 2014), E-MEXP-1928 (mature leaves; Janz et al., 2010), E-MEXP-2120 (mature leaves), E-MEXP-3741(bark; He et al., 2013), E-MEXP-2031 (developing xylem; Janz et al., 2012), E-GEOD-33977 (rays- summer and winter; Larisch et al., 2012), E-MEXP-1874 (fine roots; Luo et al., 2009), E-GEOD-43162 (fine roots; Wei et al., 2013), and for P. trichocarpa: E-GEOD-30507 (stem, shoot and leaf primordia, mature leaves, developing xylem, cambium, bark; Ko et al., 2012), E-MEXP-3910 (young leaves; Bai et al., 2013), E-GEOD-49983 (bark), E-MTAB-1483 (developing xylem and elongation zone; Euring et al., 2014), E-GEOD-21480 (stem- summer and winter); E-MEXP-3909 (young roots; Bai et al., 2013).

For the annotation of the microarray ID to the best gene model, the annotation file downloaded from Aspen Database (Tsai et al., 2011) was used.

Statistical Analyses

Statistical analyses were performed using the free statistical software R (version 3.1.1, R Core Team, 2014). One-way ANOVA was conducted for dry biomass data with plant lines (transgenic reporter lines and WT) as factor. Normality and homogeneity of variance were tested visually by plotting residuals and the data was transformed logarithmically (log₂) if needed. Data shown are mean ± SE. Means were considered to be significantly different with a p-value ≤ 0.05.

For the analyses of expression data, to summarize and normalize the array probes, ‘rma’ function from the R package ‘affy’ (Gautier et al., 2004) obtained from Bioconductor (Kauffmann et al., 2009) was used. Mean transcript abundance of the biological replicates was calculated for each gene in each tissue. When more than one probe set was present for one gene, all probe sets were used to calculate the mean value. The means were used for creating a heatmap with the ‘heatmap.2’ function from the R package ‘gplots’ (Warnes et al., 2012).

Results

The ARR5::GUS Reporter Lines Showed No Growth Differences Compared to Wild-type Poplars

The poplar lines 9, 32, and 80 were grown in ambient conditions, along with WT plants for 1.5 years. The determination of the dry mass did not show any significant difference among the lines (Table 2). There were no apparent visual differences neither in summer nor in winter (Figure 2) suggesting that the transformation with the ARR5::GUS gene construct did not hit any gene that was relevant for growth and that the expression of the construct had no influence on the plant stature.

FIGURE 2

ARR5::GUS Activity Reports a Tissue- and Cell-type Specific Pattern of Cytokinin Activity in Poplar in the Growth Phase

The localization pattern of ARR5::GUS activity during growing season was investigated in apical buds, leaf disks, root tips and in stem cross sections at three positions, i.e., top, middle, and bottom. Plants from line 9 did not display a GUS signal in any of the samples from the outdoor grown plants, suggesting that silencing had occurred. Therefore, the pictures of these samples were not considered.

In the growing season, line 32 and 80 exhibited ARR5::GUS activity in all tissues (Figure 3), except in mature leaves (not shown). In the apical buds, the ARR5::GUS activity was localized in the leaf primordia and also in the apical bud base from where the leaf primordia started (Figure 3A). ARR5::GUS was also expressed in root tips (Figure 3E). Higher magnification showed that the staining was concentrated in the root cap region, decreased in the cell division zone and was stronger again at the onset of the cell elongation zone (Figure 4). The signal showed a gradual decrease toward the direction of the shoot (Figure 4).

FIGURE 3

FIGURE 4

Examination of cytokinin activity along the stem revealed strong staining in pith in the region of the stem elongation zone, while the signal in the pith disappeared in the stem middle and at the stem bottom, where the pith was compressed by secondary growth (Figures 3B–D). In the stem middle and at the bottom the bark region below the periderm also showed a strong GUS staining (Figures 3C,D).

To investigate the cellular localization pattern of ARR5::GUS activity, stem cross sections were analyzed at a higher magnification. In the elongation zone strong ARR5::GUS activity in the pith was confirmed, but no staining was detected in the cortex or primary xylem (Figure 5A). In the middle of the stem strong GUS staining was observed only in the cortex between strands of phloem fiber cells (Figure 5B). The stem bottom sections showed a strong staining in the cortex and also in the phloem, especially at the position of the primary rays (Figure 5C). Detailed analysis in the mature xylem showed ARR5::GUS activity in the ray cells adjacent to vessels (Figure 6A). ARR5::GUS activity was also detected in the cambial zone (Figure 6C).

FIGURE 5

FIGURE 6

ARR5::GUS Activity Reports Changes in the Tissue- and Cell-type Specific Pattern of Cytokinin Activity during Dormancy Compared with the Growth Phase

During winter dormancy the apical buds showed no ARR5::GUS activity in the leaf primordia, but a very strong signal in the ground tissue below the bud base (Figure 7A). ARR5::GUS activity below the bud base extended into a larger area of the ground tissue of the stem than that observed during the growing season.

FIGURE 7

Below the apex, at the stem top ARR5::GUS activity was detected in the pith, however, with weaker intensity than in summer (Figure 7B). This signal disappeared in the stem middle and bottom (Figures 7C,D). The stem middle and bottom cross sections showed a strong staining in the bark region (Figures 7C,D). In the root tips ARR5::GUS activity was absent in winter (Figure 7E).

Cellular localization of ARR5::GUS activity was also monitored along the dormant stem (Figure 8). The stem top section, which was collected at the same position below the apex as in summer, showed a fully developed circular ring of secondary xylem, indicating that secondary growth had already started in this zone (Figure 8A). The reason is that after bud set in fall, elongation growth stops and the undifferentiated ground tissues continue to develop for some time. In winter, ARR5::GUS activity was present mainly in the outer pith region, the perimedullary zone (Figure 8A), whereas in the stem top sections from the growth phase most of the ARR5::GUS activity was localized in the center of the pith. In the stem middle, anatomy and the pattern of ARR5::GUS activity were similar to that at the stem bottom (Figures 8B,C). The ARR5::GUS activity extended across the whole cortex region of the bark and therefore, was stronger than in summer in this tissue (Figures 8B,C). Similar as in summer, the staining was pronounced in cell files that were connected with xylem rays (Figures 8B,C). ARR5::GUS activity was also localized in the cambial zone at the stem bottom during dormancy with a stronger signal than that detected in summer (Figure 6D).

FIGURE 8

Similar as in summer, the ray cells adjacent to vessels showed ARR5::GUS activity (Figure 6B), but the stain was less pronounced than in summer (Figure 6A). The ARR5::GUS activity in distinct locations of the xylem rays was only present at the stem bottom.

Tissue Specific Expression Pattern of PtRR Type-A Genes

The expression of genes belonging to the type-A RR family in poplar was analyzed in different tissues employing microarray data (Figure 9). Each of the 11 genes identified in poplar (Ramírez-Carvajal et al., 2008, cf. Table 1) had a probe set on the microarrays and therefore could be included here. There was no clustering of the PtRR transcriptional pattern according to tissues (not shown), but all tissues showed an expression of all PtRR type-A genes (Figure 9). The PtRR transcriptional pattern clearly clustered the genes in two categories, one comprising genes with low expression (PtRR8, 9, and 11) and the other with genes that showed variable expression across the tissues and season (PtRR1, 2, 3, 4, 5, 6, 7, and 10). PtRR10, the ortholog of ARR5, was expressed in all tissues under study, especially in the phloem and elongation zone in the growth phase (Figure 9), thus supporting consistency between PtRR10 expression and our reporter lines. In the fine roots from the growth phase, mainly PtRR10 was expressed while in the young roots PtRR5 was also expressed. PtRR5, 3, and 1 showed strong expression in the phloem during the growth phase. In the cambium tissues during summer, PtRR10 and 5 were mainly expressed. In developing xylem, during growth phase, PtRR10, 5, and 6 showed strong expression. PtRR5 showed a strong expression in the developing xylem in summer followed by PtRR10 and 4. In the summer rays, PtRR10 and 1 showed strong expression. PtRR7 was also expressed in summer ray cells. But in winter rays, only PtRR1 showed strong expression. In the elongation zone of the stem, during the growth phase, PtRR10, 5, 3, 6, 1, and 5 were strongly expressed. In the shoot apex only PtRR10 was strongly expressed.

FIGURE 9

Discussion

The ARR5::GUS Construct Is Functional in Poplar

Many of the biological and developmental phenomena shared by herbaceous and woody plants are regulated by the same molecular mechanism. Besides having the same cytokinin signal transduction components, the type-ARR gene family found in Arabidopsis and poplar is well conserved in these two plant species (Immanen et al., 2013) with the highest similarity between PtRR10 and ARR5 (Ramírez-Carvajal et al., 2008; Immanen et al., 2013). Here, we show that the ARR5::GUS reporter construct was functional in poplar because it was inducible by the cytokinin analogs, thidiazuron and BAP and not by adenine, an inactive cytokinin analog. Thus, ARR5::GUS transformed poplar lines record the distribution of active cytokinins selectively. However, quantification of the signal is not possible because of the unknown turnover of GUS and the produced indigo dye.

Normal growth of the transgenic poplars indicated that there was no significant non-target effect of the biotechnological modification on plant performance. However, in one of the three reporter lines, ARR5::GUS construct was apparently silenced during long-term growth under ambient conditions. Silencing is not uncommon in transgenic plants and can have a number of different reasons (Stam et al., 1997; Fagard and Vaucheret, 2000). The synthetic promoter construct TCS::GFP for monitoring cytokinin in Arabidopsis was also subjected to silencing (Zürcher et al., 2013). Here, the two active reporter lines showed similar patterns of the ARR5::GUS activity in those tissues that also showed PtRR10 expression, thus, supporting that they confidently recorded cytokinin activity.

The Localization of ARR5::GUS Identifies Novel Cytokinin-Active Cell Types in Poplar

In the growing phase, the main tissues with strong ARR5::GUS activity included the apical bud base, the root tips, pith in stem elongation zone, and bark in the stem middle and bottom. The localization of active cytokinins in the apical bud base and in root tips shows strong similarity to that observed in ARR5::GUS expressing Arabidopsis seedlings (D’Agostino et al., 2000). In Arabidopsis seedlings, the primary and lateral root tips showed strong ARR5::GUS activity in the root cap region as well as in the cell division region and elongation zone with a gradual decrease toward the direction of the shoot apex (D’Agostino et al., 2000). This staining pattern was also evident in the growing season in poplar root tips in our study. Aloni et al. (2004) reported that the ARR5::GUS signal in Arabidopsis roots was produced in the statocytes.

In the stem top, the observation of ARR5::GUS activity in the pith was unexpected, but similarly Teichmann et al. (2008) also had found auxin activity in this tissue. This finding suggests that the pith may have an important function for the hormone supply in the young stem, where the vascular system is not yet fully differentiated. Active cytokinins were also detected in the cambial zone of poplar, in agreement with studies showing that cytokinins are important regulators of cambial activity in growing poplars (Matsumoto-Kitano et al., 2008; Nieminen et al., 2008).

The presence of active cytokinins in the xylem ray cells, which was detected here, has not been reported so far, but was underpinned by high expression of poplar PtRR10, PtRR1, and PtRR7 in this cell type. A noteworthy finding was that the ARR5::GUS activity in the ray cells was seen only in parts associated with vessels. The biological significance of high cytokinin activity close to the vessels is unknown. However, root-derived cytokinin that are transported with the xylem sap through the vessel, are likely to be supplied by this route to the rays.

The ARR5::GUS staining pattern observed in the bark cortical cells, primary rays and in the cambium support previous studies reporting that cytokinins are necessary for determining vascular cell identities (Mähönen et al., 2000; Hutchison et al., 2006; Yokoyama et al., 2007; Argyros et al., 2008) and stimulate growth (Werner et al., 2003). The positional pattern of active cytokinin found here agrees with strong expression of the type-ARR genes PtRR3, PtRR5, and PtRR10) in the phloem of greenhouse grown P. trichocarpa (Ramírez-Carvajal et al., 2008). Furthermore, we found strong expression of PtRR10, PtRR5, PtRR3, and PtRR1 in bark tissues. In the cambium PtRR10 and PtRR5 were highly expressed indicating responsiveness to cytokinins.

The reporter lines generally show expected localization pattern, but an exception was also noted. Although PtRR10 was expressed in mature leaves, no ARR5::GUS activity was found in these tissues. One possibility is that PtRR10 transcription is regulated by further signals to which ARR5 is not responsive or the ARR5::GUS reporter construct is insensitive to cytokinins in fully expanded leaves in summer. Similar cases have been reported for poplar auxin reporter lines transformed with GH3::GUS (Teichmann et al., 2008) and DR5::GUS (Chen et al., 2013), where no activity of these constructs was noted in the cambium, a tissue in which an auxin maximum is expected. However, in our study ARR5::GUS activity was detected in the leaf primordia, where cytokinin levels determine leaf size (Holst et al., 2011)

A comparison of ARR5::GUS activity along the stem with that of auxin reporter lines (GH3::GUS, DR5::GUS, Teichmann et al., 2008; Chen et al., 2013) shows overlap of the hormone activities in the elongation zone, but contrasting intensities in the bark. In ARR5::GUS poplars, the staining in bark was stronger toward the stem base, whereas that of auxin reporter lines decreased toward the base. These observations indicate that the phytohormone reporter lines also truly reflect the hormone gradients installed by the opposite apical production sites in roots for cytokinins and in the stem for auxin and the inverse transport pattern of these phytohormones along the stem (Jones and Ljung, 2011).

Cytokinin Activity Is Subject to Seasonal Fluctuations in Distinct Tissues

Seasonal fluctuation of cytokinin activity was most notable in the root tips, the major site of cytokinin synthesis (Aloni et al., 2004, 2005). The absence of ARR5::GUS activity in the root tips during dormancy together with a strong presence of ARR5::GUS activity in the apical bud base, in the pith, and in the bark suggests that the active cytokinins in these shoot tissues may be shoot-derived rather than root-derived. Cytokinins from different production sites have been distinguished by their chemical composition. Root-derived cytokinins are mainly of the trans-zeatin (tZ) type (Aloni et al., 2005), whereas phloem-transported isopentenyladenine (iP) type cytokinins are considered to be shoot-derived (Bishopp et al., 2011). In winter, the concentration of tZ is low in the xylem sap of willows, probably because of their decreased root production (Alvim et al., 1976), which corresponds to the lacking ARR5::GUS signal in our study. At the start of dormancy very high levels of the iP type are present in the phloem sap of 14 different tree species (Weiler and Ziegler, 1981). The studies with excised twigs of Populus × robusta and rootless almond shoots also confirm the presence of cytokinin after chilling (Hewett and Wareing, 1973; Van Staden and Dimalla, 1981). Increased cytokinin levels of the bark and in buds before the bud burst were further reported in artificially chilled, excised apple shoots (Cook et al., 2001). All these studies suggested that shoot derived cytokinins play a role in dormancy and the following bud burst in spring. The source of these cytokinins could be de novo biosynthesis or conversion of storage forms to their active forms (Skene, 1972; Kannangara and Booth, 1974; Van Staden and Brown, 1978; Van Staden, 1979; Van Staden and Dimalla, 1981). Collectively, these studies show that cytokinins are present in the dormant phase and together with our results on ARR5::GUS activity, it is clear that they are active in distinct cell types such as cortical and ray cells in the bark, pith, and ray cells next to vessels and in the shoot apex. In the cambial zone, a strong staining was also detected during dormancy. This observation may suggest a role of cytokinins in cambial cell maintenance in winter.

Conclusion

Employing an ARR5::GUS reporter, we monitored seasonal differences and similarities of cytokinin activity at the tissue and cellular level in poplar. Since cytokinins increase the sensitivity of the cambium to the auxin signal, they are important regulators of wood quantity and quality (Aloni, 1991, 2001). Therefore, the reporter lines can be used to investigate the involvement of cytokinins in mediating growth constraints and growth-promoting treatments for vascular development and cell type identities in the future. Thereby, these poplars may become an important tool to enhance our understanding of woody biomass production.

References

• 1

  AloniR. (1991). “Wood formation in deciduous hardwood trees,” inPhysiology of Treesed.RaghavendraA. S. (New York, NY: Wiley) 175–197.

  • Google Scholar
  • View reference in article

• 2

  AloniR. (2001). Foliar and axial aspects of vascular differentiation: hypotheses and evidence.J. Plant Growth Regul.2022–34. 10.1007/s003440010001

  • CrossRef
  • Google Scholar
  • View reference in article

• 3

  AloniR.LanghansM.AloniE.DreieicherE.UllrichC. I. (2005). Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream.J. Exp. Bot.561535–1544. 10.1093/jxb/eri148

  • CrossRef
  • Google Scholar
  • View reference in article

• 4

  AloniR.LanghansM.AloniE.UllrichC. I. (2004). Role of cytokinin in the regulation of root gravitropism.Planta220177–182. 10.1007/s00425-004-1381-8

  • CrossRef
  • Google Scholar
  • View reference in article

• 5

  AlvimR.HewettE. W.SaundersP. F. (1976). Seasonal variation in the hormone content of Willow: I. changes in abscisic acid content and cytokinin activity in the xylem Sap 1.Plant physiol.57474–476. 10.1104/pp.57.4.474

  • CrossRef
  • Google Scholar
  • View reference in article

• 6

  ArgyrosR. D.MathewsD. E.ChiangY.-H.PalmerC. M.ThibaultD. M.EtheridgeN.et al (2008). Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development.The Plant Cell Online202102–2116. 10.1105/tpc.108.059584

  • CrossRef
  • Google Scholar
  • View reference in article

• 7

  BaiH.EuringD.VolmerK.JanzD.PolleA. (2013). The nitrate transporter (NRT) gene family in poplar.PLoS ONE8:e72126. 10.1371/journal.pone.0072126

  • CrossRef
  • Google Scholar
  • View reference in article

• 8

  BishoppA.HelpH.El-ShowkS.WeijersD.ScheresB.FrimlJ.et al (2011). A mutually inhibitory interaction between auxin and cytokinin specifies vascular pattern in roots.Curr. Biol.21917–926. 10.1016/j.cub.2011.04.017

  • CrossRef
  • Google Scholar
  • View reference in article

• 9

  ChenY.YordanovY. S.MaC.StraussS.BusovV. B. (2013). DR5 as a reporter system to study auxin response in Populus.Plant Cell Rep.32453–463. 10.1007/s00299-012-1378-x

  • CrossRef
  • Google Scholar
  • View reference in article

• 10

  CookN. C.BellstedtD. U.JacobsG. (2001). Endogenous cytokinin distribution patterns at budburst in Granny Smith and Braeburn apple shoots in relation to bud growth.Sci. Hortic.8753–63. 10.1016/S0304-4238(00)00161-8

  • CrossRef
  • Google Scholar
  • View reference in article

• 11

  D’AgostinoI. B.DeruèreJ.KieberJ. J. (2000). Characterization of the response of the Arabidopsis response regulator gene family to cytokinin.Plant Physiol.1241706–1717. 10.1104/pp.124.4.1706

  • CrossRef
  • Google Scholar
  • View reference in article

• 12

  DielemanJ. A.VerstappenF. W. A.NicanderB.KuiperD.TillbergE.TrompJ. (1997). Cytokinins in Rosa hybrida in relation to bud break.Physiol. Plant.99456–464. 10.1111/j.1399-3054.1997.tb00560.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 13

  EuringD.BaiH.JanzD.PolleA. (2014). Nitrogen-driven stem elongation in poplar is linked with wood modification and gene clusters for stress, photosynthesis and cell wall formation.BMC Plant Biol.14:391. 10.1186/s12870-014-0391-3

  • CrossRef
  • Google Scholar
  • View reference in article

• 14

  FagardM.VaucheretH. (2000). (Trans) gene silencing in plants: how many mechanisms?Annu. Rev. Plant Physiol. Plant Mol. Biol.51167–194. 10.1146/annurev.arplant.51.1.167

  • CrossRef
  • Google Scholar
  • View reference in article

• 15

  GautierL.CopeL.BolstadB. M.IrizarryR. A. (2004). Affy-analysis of Affymetrix GeneChip data at the probe level.Bioinformatics20307–315. 10.1093/bioinformatics/btg405

  • CrossRef
  • Google Scholar
  • View reference in article

• 16

  GoodsteinD. M.ShuS.HowsonR.NeupaneR.HayesR. D.FazoJ.et al (2012). Phytozome: a comparative platform for green plant genomics.Nucleic Acids Res.40D1178–D1186. 10.1093/nar/gkr944

  • CrossRef
  • Google Scholar
  • View reference in article

• 17

  HeJ.LiH.LuoJ.MaC.LiS.QuL.et al (2013). A transcriptomic network underlies microstructural and physiological responses to cadmium in Populus × canescens.Plant Physiol.162424–439. 10.1104/pp.113.215681

  • CrossRef
  • Google Scholar
  • View reference in article

• 18

  HewettE. W.WareingP. F. (1973). Cytokinins in Populus × robusta Schneid: a complex in leaves.Planta112225–233. 10.1007/BF00385326

  • CrossRef
  • Google Scholar
  • View reference in article

• 19

  HiroseN.TakeiK.KurohaT.Kamada-NobusadaT.HayashiH.SakakibaraH. (2008). Regulation of cytokinin biosynthesis, compartmentalization and translocation.J. Exp. Bot.5975–83. 10.1093/jxb/erm157

  • CrossRef
  • Google Scholar
  • View reference in article

• 20

  HolstK.SchmüllingT.WernerT. (2011). Enhanced cytokinin degradation in leaf primordia of transgenic Arabidopsis plants reduces leaf size and shoot organ primordia formation.J. Plant Physiol.1681328–1334. 10.1016/j.jplph.2011.03.003

  • CrossRef
  • Google Scholar
  • View reference in article

• 21

  HutchisonC. E.LiJ.ArguesoC.GonzalezM.LeeE.LewisM. W.et al (2006). The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling.Plant Cell Online183073–3087. 10.1105/tpc.106.045674

  • CrossRef
  • Google Scholar
  • View reference in article

• 22

  HwangI.SheenJ.MüllerB. (2012). Cytokinin signaling networks.Annu. Rev. Plant Biol.63353–380. 10.1146/annurev-arplant-042811-105503

  • CrossRef
  • Google Scholar
  • View reference in article

• 23

  ImmanenJ.NieminenK.SilvaH. D.RojasF. R.MeiselL. A.SilvaH.et al (2013). Characterization of cytokinin signaling and homeostasis gene families in two hardwood tree species: Populus trichocarpa and Prunus persica.BMC Genomics14:885. 10.1186/1471-2164-14-885

  • CrossRef
  • Google Scholar
  • View reference in article

• 24

  JanzD.BehnkeK.SchnitzlerJ.-P.KanawatiB.Schmitt-KopplinP.PolleA. (2010). Pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms.BMC Plant Biol.10:150. 10.1186/1471-2229-10-150

  • CrossRef
  • Google Scholar
  • View reference in article

• 25

  JanzD.LautnerS.WildhagenH.BehnkeK.SchnitzlerJ.-P.RennenbergH.et al (2012). Salt stress induces the formation of a novel type of pressure wood in two Populus species.New Phytol.194129–141. 10.1111/j.1469-8137.2011.03975.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 26

  JeffersonR. A.KavanaghT. A.BevanM. W. (1987). GUS fusions: β-Glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J.63901–3907.

  • Google Scholar
  • View reference in article

• 27

  JonesB.LjungK. (2011). Auxin and cytokinin regulate each other’s levels via a metabolic feedback loop.Plant Signal. Behav.6901–904. 10.4161/psb.6.6.15323

  • CrossRef
  • Google Scholar
  • View reference in article

• 28

  Kamada-NobusadaT.SakakibaraH. (2009). Molecular basis for cytokinin biosynthesis.Phytochemistry70444–449. 10.1016/j.phytochem.2009.02.007

  • CrossRef
  • Google Scholar
  • View reference in article

• 29

  KannangaraT.BoothA. (1974). Diffusible cytokinins in shoot apices of Dahlia variabilis.J. Exp. Bot.25459–467. 10.1093/jxb/25.2.459

  • CrossRef
  • Google Scholar
  • View reference in article

• 30

  KauffmannA.RaynerT. F.ParkinsonH.KapusheskyM.LukkM.BrazmaA.et al (2009). Importing ARRAYEXPRESS datasets into R/ bioconductor.Bioinformatics252092–2094. 10.1093/bioinformatics/btp354

  • CrossRef
  • Google Scholar
  • View reference in article

• 31

  KieberJ. J.SchallerG. E. (2014). Cytokinins.Arabidopsis Book12:e0168. 10.1199/tab.0168

  • CrossRef
  • Google Scholar
  • View reference in article

• 32

  KoJ.-H.KimH.-T.HwangI.HanK.-H. (2012). Tissue-type-specific transcriptome analysis identifies developing xylem-specific promoters in poplar: xylem-specificutility promoters in poplar.Plant Biotechnol. J.10587–596. 10.1111/j.1467-7652.2012.00690.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 33

  KolesnikovN.HastingsE.KeaysM.MelnichukO.TangY. A.WilliamsE.et al (2015). ArrayExpress update-simplifying data submissions.Nucleic Acids Res.43D1113–D1116. 10.1093/nar/gku1057

  • CrossRef
  • Google Scholar
  • View reference in article

• 34

  LarischC.DittrichM.WildhagenH.LautnerS.FrommJ.PolleA.et al (2012). Poplar wood rays are involved in seasonal remodeling of tree physiology.Plant Physiol.1601515–1529. 10.1104/pp.112.202291

  • CrossRef
  • Google Scholar
  • View reference in article

• 35

  LepleJ. C.BrasileiroA. C. M.MichelM. F.DelmotteF.JouaninL. (1992). Transgenic poplars: expression of chimeric genes using four different constructs.Plant Cell Rep.11137–141. 10.1007/BF00232166

  • CrossRef
  • Google Scholar
  • View reference in article

• 36

  LuoZ.-B.JanzD.JiangX.GobelC.WildhagenH.TanY.et al (2009). Upgrading root physiology for stress tolerance by ectomycorrhizas: insights from metabolite and transcriptional profiling into reprogramming for stress anticipation.Plant Physiol.1511902–1917. 10.1104/pp.109.143735

  • CrossRef
  • Google Scholar
  • View reference in article

• 37

  MähönenA. P.BonkeM.KauppinenL.RiikonenM.BenfeyP. N.HelariuttaY. (2000). A novel two-component hybrid molecule regulates vascular morphogenesis of the Arabidopsis root.Genes Dev.142938–2943. 10.1101/gad.189200

  • CrossRef
  • Google Scholar
  • View reference in article

• 38

  Matsumoto-KitanoM.KusumotoT.TarkowskiP.Kinoshita-TsujimuraK.VáclavíkováK.MiyawakiK.et al (2008). Cytokinins are central regulators of cambial activity.Proc. Natl. Acad. Sci. U.S.A.10520027–20031. 10.1073/pnas.0805619105

  • CrossRef
  • Google Scholar
  • View reference in article

• 39

  MiyawakiK.Matsumoto-KitanoM.KakimotoT. (2004). Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate.Plant J.37128–138. 10.1046/j.1365-313X.2003.01945.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 40

  MizunoT. (2005). Two-component phosphorelay signal transduction systems in plants: from hormone responses to circadian rhythms.Biosci. Biotechnol. Biochem.692263–2276. 10.1271/bbb.69.2263

  • CrossRef
  • Google Scholar
  • View reference in article

• 41

  MokD. W.MokM. C. (2001). Cytokinin metabolism and action.Annu. Rev. Plant Physiol. Plant Mol. Biol.5289–118. 10.1146/annurev.arplant.52.1.89

  • CrossRef
  • Google Scholar
  • View reference in article

• 42

  MüllerA.VolmerK.Mishra-KnyrimM.PolleA. (2013). Growing poplars for research with and without mycorrhizas.Front. Plant Sci.4:332. 10.3389/fpls.2013.00332

  • CrossRef
  • Google Scholar
  • View reference in article

• 43

  NieminenK.ImmanenJ.LaxellM.KauppinenL.TarkowskiP.DolezalK.et al (2008). Cytokinin signaling regulates cambial development in poplar.Proc. Natl. Acad. Sci. U.S.A.10520032–20037. 10.1073/pnas.0805617106

  • CrossRef
  • Google Scholar
  • View reference in article

• 44

  PilsB.HeylA. (2009). Unraveling the evolution of cytokinin signaling.Plant Physiol.151782–791. 10.1104/pp.109.139188

  • CrossRef
  • Google Scholar
  • View reference in article

• 45

  R Core Team (2014). R: A Language and Environment for Statistical Computing.Vienna: R foundation for statistical computing. Available at: http://www.R-project.org/

  • Google Scholar
  • View reference in article

• 46

  Ramírez-CarvajalG. A.MorseA. M.DavisJ. M. (2008). Transcript profiles of the cytokinin response regulator gene family in Populus imply diverse roles in plant development.New Phytol.17777–89. 10.1111/j.1469-8137.2007.02240.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 47

  RomanovG. A.LominS. N.SchmullingT. (2006). Biochemical characteristics and ligand-binding properties of Arabidopsis cytokinin receptor AHK3 compared to CRE1/AHK4 as revealed by a direct binding assay.J. Exp. Bot.574051–4058. 10.1093/jxb/erl179

  • CrossRef
  • Google Scholar
  • View reference in article

• 48

  SakakibaraH. (2006). Cytokinins: activity, biosynthesis, and translocation.Annu. Rev. Plant Biol.57431–449. 10.1146/annurev.arplant.57.032905.105231

  • CrossRef
  • Google Scholar
  • View reference in article

• 49

  SchallerG. E.KieberJ. J.ShiuS.-H. (2008). Two-component signaling elements and histidyl-aspartyl phosphorelays.Arabidopsis Book6:e0112. 10.1199/tab.0112

  • CrossRef
  • Google Scholar
  • View reference in article

• 50

  SkeneK. G. (1972). Cytokinins in the xylem sap of grape vine canes: changes in activity during cold-storage.Planta10489–92. 10.1007/BF00387686

  • CrossRef
  • Google Scholar
  • View reference in article

• 51

  StamM.MolJ. N.KooterJ. M. (1997). The silence of genes in transgenic plants.Ann. Bot.793–12. 10.1006/anbo.1996.0295

  • CrossRef
  • Google Scholar
  • View reference in article

• 52

  TanakaM.TakeiK.KojimaM.SakakibaraH.MoriH. (2006). Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance.Plant J.451028–1036. 10.1111/j.1365-313X.2006.02656.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 53

  TaniguchiM.KibaT.SakakibaraH.UeguchiC.MizunoT.SugiyamaT. (1998). Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate.FEBS Lett.429259–262. 10.1016/S0014-5793(98)00611-5

  • CrossRef
  • Google Scholar
  • View reference in article

• 54

  TeichmannT.Bolu-AriantoW. H.OlbrichA.Langenfeld-HeyserR.GöbelC.GrzeganekP.et al (2008). GH3::GUS reflects cell-specific developmental patterns and stress-induced changes in wood anatomy in the poplar stem.Tree Physiol.281305–1315. 10.1093/treephys/28.9.1305

  • CrossRef
  • Google Scholar
  • View reference in article

• 55

  TrompJ.OvaaJ. C. (1990). Seasonal changes in the cytokinin composition of xylem sap of Apple.J. Plant Physiol.136606–610. 10.1016/S0176-1617(11)80221-X

  • CrossRef
  • Google Scholar
  • View reference in article

• 56

  TsaiC. J.RanjanP.DiFazioS. P.TuskanG. A.JohnsonV. (2011). “Poplar genome microarrays,” inGenetics, Genomics and Breeding of PoplarsedsJoshiC. P.DiFazioS. P.KoleC. (Enfield: Science Publishers) 112–127.

  • Google Scholar
  • View reference in article

• 57

  Van StadenJ. (1979). Changes in the endogenous cytokinin levels of excised buds of Salix babylonica L., cultured aseptically.Bot. Gaz.140138–141. 10.1086/337069

  • CrossRef
  • Google Scholar
  • View reference in article

• 58

  Van StadenJ.BrownN. A. C. (1978). Changes in the endogenous cytokinins of bark and buds of Salix babylonica as a result of stem girdling.Physiol. Plant.43148–153. 10.1111/j.1399-3054.1978.tb01583.x

  • CrossRef
  • Google Scholar
  • View reference in article

• 59

  Van StadenJ.DimallaG. G. (1981). The production and utilisation of cytokinins in rootless, dormant Almond shoots maintained at low temperature.Z. Pflanzenphysiol.103121–129. 10.1016/S0044-328X(81)80141-9

  • CrossRef
  • Google Scholar
  • View reference in article

• 60

  WarnesG. R.BolkerB.BonebakkerL.GentlemanR.LiawW. H. A.LumleyT.et al (2012). Gplots: Various R Programming Tools for Plotting Data.Available at: http://CRAN.R-project.org/package=gplots

  • Google Scholar
  • View reference in article

• 61

  WeiH.YordanovY. S.GeorgievaT.LiX.BusovV. (2013). Nitrogen deprivation promotes Populus root growth through global transcriptome reprogramming and activation of hierarchical genetic networks.New Phytol.200483–497. 10.1111/nph.12375

  • CrossRef
  • Google Scholar
  • View reference in article

• 62

  WeilerE. W.ZieglerH. (1981). Determination of phytohormones in phloem exudate from tree species by radioimmunoassay.Planta152168–170. 10.1007/BF00391189

  • CrossRef
  • Google Scholar
  • View reference in article

• 63

  WernerT.MotykaV.LaucouV.SmetsR.Van OnckelenH.SchmüllingT. (2003). Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity.Plant Cell Online152532–2550. 10.1105/tpc.014928

  • CrossRef
  • Google Scholar
  • View reference in article

• 64

  YokoyamaA.YamashinoT.AmanoY.-I.TajimaY.ImamuraA.SakakibaraH.et al (2007). Type-B ARR transcription factors, ARR10 and ARR12, are implicated in cytokinin-mediated regulation of protoxylem differentiation in roots of Arabidopsis thaliana.Plant Cell Physiol.4884–96. 10.1093/pcp/pcl040

  • CrossRef
  • Google Scholar
  • View reference in article

• 65

  YordanovY. S.MaC.StraussS. H.BusovV. B. (2014). EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees.Proc. Natl. Acad. Sci. U.S.A.11110001–10006. 10.1073/pnas.1405621111

  • CrossRef
  • Google Scholar
  • View reference in article

• 66

  ZürcherE.Tavor-DeslexD.LituievD.EnkerliK.TarrP. T.MüllerB. (2013). A robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin signaling network in planta.Plant Physiol.1611066–1075. 10.1104/pp.112.211763

  • CrossRef
  • Google Scholar
  • View reference in article