Abstract
Daylength is perceived as a seasonal cue to induce growth-phase transition at a proper time of a year. The core of the mechanism of daylength measurement in angiosperms lies in the circadian clock-controlled expression of regulators of growth-phase transition. However, the roles of the circadian clock in daylength measurement in basal land plants remain largely unknown. In this study, we investigated the contribution of circadian clock to daylength measurement in a basal land plant, the liverwort Marchantia polymorpha. In M. polymorpha, transition from vegetative to reproductive phase under long-day conditions results in differentiation of sexual branches called gametangiophores which harbor gametangia. First, we showed that a widely used wild-type accession Takaragaike-1 is an obligate long-day plant with a critical daylength of about 10 hours and requires multiple long days. Then, we compared the timing of gametangiophore formation between wild type and circadian clock mutants in long-day and short-day conditions. Mutations in two clock genes, MpTIMING OF CAB EXPRESSION 1 and MpPSEUDO-RESPONSE REGULATOR, had no significant effects on the timing of gametangiophore formation. In addition, when M. polymorpha plants were treated with a chemical which lengthens circadian period, there was no significant effect on the timing of gametangiophore formation, either. We next observed the timing of gametangiophore formation under various non-24-h light/dark cycles to examine the effect of phase alteration in circadian rhythms. The results suggest that daylength measurement in M. polymorpha is based on the relative amount of light and darkness within a cycle rather than the intrinsic rhythms generated by circadian clock. Our findings suggest that M. polymorpha has a daylength measurement system which is different from that of angiosperms centered on the circadian clock function.
Introduction
To increase reproductive fitness, it is crucial for plants to undergo the growth-phase transition from vegetative to reproductive phase at the suitable season with favorable environmental conditions. Many plants measure daylength as the most reliable seasonal cue to ensure the proper timing of the growth-phase transition. According to the external coincidence model, daylength measurement is based on the interaction of the endogenous circadian clock with the environmental light signal (; ). The circadian clock sets a light-sensitive phase by regulating the expression of some key regulators. When light signal coincides with the light-sensitive phase, daylength-dependent response is induced. The mechanism of daylength measurement based on the external and internal coincidence models in plants is best studied in Arabidopsis thaliana (Arabidopsis) (). Arabidopsis is a facultative long-day (LD) plant, in which flowering is promoted under LD conditions, but eventually occurs under short days as well. In Arabidopsis, the timing of the expression peak of CONSTANS (CO), the transcriptional activator of a florigen gene FLOWERING LOCUS T (FT), is of critical importance for daylength measurement (). The CO expression is regulated by GIGANTEA (GI) and FLAVIN-BINDING KELCH DOMAIN F-BOX PROTEIN 1 (FKF1). Both GI and FKF1 expression is regulated by the circadian clock, and the two proteins interact with each other in the presence of blue light. The GI-FKF1 complex degrades transcriptional repressors, CYCLING DOF FACTORs (CDFs), thereby allowing CO transcription (; ; ). Under LD conditions, the coincidence of high expression of CO and light induces the expression of FT (; ; ).
Mutations in the genes of the circadian-clock components often affect flowering time ( [errata, 2013]). This is explained by the change of the timing of CO expression (; ). For example, toc1-1, a mutant of TIMING OF CAB EXPRESSION 1 (TOC1) with a shorter circadian period, showed an early flowering phenotype under short-day (SD) conditions (). The shorter circadian period results in earlier (phase-advanced) CO expression, which causes the coincidence of high expression of CO and light even under SD conditions and consequently induces FT expression and flowering (). used non-24h light/dark cycles to alter the timing of circadian gene expression. They grew wild-type (WT) plants under light/dark cycles shorter or longer than 24 h comprised of a fixed light-to-dark ratio (in this case, 1 to 2) referred to as T-cycles, which caused later or earlier CO expression. Similar to the observations in the toc-1-1 mutant, flowering was accelerated when CO was expressed in the light portion of the cycle. These results together show that the interaction between the circadian clock and light signal is the basis of daylength measurement in Arabidopsis. Similarly, it has been reported that several angiosperm species measure daylength through the interaction between the circadian clock and light signal (). Hence, it has been believed that this daylength measurement system based on the external and internal coincidence mechanisms is conserved in a wide range of plants. In contrast, in non-angiosperm plants, the mechanisms of daylength measurement are poorly understood.
To expand our knowledge of the mechanism of daylength measurement, we used an LD plant, Marchantia polymorpha s.sp. ruderalis (hereafter referred to as M. polymorpha). A model liverwort, M. polymorpha is suitable for genetic studies because of its low genetic redundancy of regulatory genes and various molecular biological techniques available (; ; ). In M. polymorpha, growth-phase transition from vegetative to reproductive phase results in differentiation of gametangia and gametangiophores, Marchantiidae-specific stalked structures harboring gamatangia (; ; ). Since liverworts represent one of the earliest diverged lineages of extant land plants (), studies with M. polymorpha have an additional merit of enhancing our understanding of the evolution of daylength-measurement mechanisms.
M. polymorpha lacks the CO ortholog and the counterpart of FT is also missing. But it possesses the single-copy ortholog or counterpart of GI, FKF1, and CDF, respectively (; ; ). Recent studies demonstrated that the GI-FKF1 module is involved in the regulation of daylength-dependent growth-phase transition in M. polymorpha. Knockout of either MpFKF or MpGI suppressed the growth-phase transition irrespective of day-length conditions, while overexpression of either MpFKF or MpGI caused the growth-phase transition under non-inductive SD conditions (). Moreover, MpGI showed a diel expression pattern similar to its Arabidopsis orthologue. These imply that, as in Arabidopsis, the circadian clock may be the key element in daylength measurement in M. polymorpha. Recent studies showed that M. polymorpha has a circadian oscillator that shares many components with Arabidopsis and there is the circadian regulation of nyctinastic thallus movement and photosynthesis (; ; ). However, it still remains to be examined whether the circadian clock is essential for daylength-dependent growth-phase transition in M. polymorpha.
In this study, we aimed to examine the involvement of the circadian clock in the regulation of daylength-dependent growth-phase transition through genetic (clock mutants), pharmacological (an inhibitor of a clock component), and physiological (non-24h light/dark cycles) approaches. All the results consistently indicate that the circadian clock is not the key element in daylength measurement of M. polymorpha. Moreover, our observations suggest that M. polymorpha responds to daylength based on the relative amount of light and darkness within a cycle. Our study provides new insights into evolution and diversification of daylength measurement systems in land plants.
Materials and methods
Plant materials and growth conditions
A male accession of M. polymorpha, Takaragaike-1 (Tak-1) was used as WT. Plants were grown on half-strength Gamborg’s B5 medium () containing 1% sucrose and 1.2% agar under 50–60 µmol photons m−2 s−1 continuous white fluorescent light at 22°C, unless otherwise specified (e.g. for non-24-h light/dark cycles). Mutants generated in this study by genome editing were named in accordance with the research community’s guidelines ().
Induction of growth-phase transition
Gemmae were plated on half-strength Gamborg’s B5 medium containing 1% sucrose and 1.2% agar and kept in the dark for one day and then exposed to white fluorescent light with 50–60 μmol m−2 s−1 for an hour to synchronize germination. Then, gemmalings were transferred to non-inductive 8-hour light (L)/16-hour dark (D) (8L16D) conditions using 50–60 μmol m−2 s−1 white light supplemented with 45–50 μmol m−2 s−1 far-red light-emitting diodes (LED Profile Light for Plant, NAMOTO, Japan; peak emission at 730-735 nm). Ten days after growth under 8L16D conditions, they were transferred to various day-length conditions (see Supplementary Figure 1). The number of days required for the appearance of the first visible gametangiophore primordium after the transfer to various day-length conditions was scored.
Construction of plasmids
The genomic sequence of the MpPSEUDO-RESPONSE REGULATOR (MpPRR) promoter region (2974 bp) was amplified from Tak-1 genomic DNA using primers: 5′-ATCCGGTACCGAATTCATAAGACGTTTGATGTGGCAATGG-3′ and 5′-GTGCGGCCGCGAATTCATCAGAAGAGAAATTGCAGTACG-3′. The amplified fragment was cloned into the EcoRI site of pENTR1A vector (Thermo Fisher Scientific). The inserted fragments were then transferred into pMpGWB331 () to generate proMpPRR : LUC plasmid.
To generate mutants for MpTOC and MpPRR, oligonucleotides: MpTOC_sgRNA1, 5′-CTCGTCAACCTCGCCGAAGTGTCA-3′ and 5′-AAACTGACACTTCGGCGAGGTTGA-3′, MpTOC_sgRNA2, 5′- CTCGACACACAAGGAAACCCTGCT-3′ and 5′- AAACAGCAGGGTTTCCTTGTGTGT-3′, MpPRR_sgRNA1, 5′- CTCGTGCAGAGTCCAGTGAGAAGG-3′ and 5′- AAACCCTTCTCACTGGACTCTGCA-3′, MpPRR_sgRNA2, 5′- CTCGAGCGGACTTAACGTGAGAGG-3′ and 5′- AAACCCTCTCACGTTAAGTCCGCT-3′ were used as guide RNA (gRNA) sequences for CRISPR/Cas9-mediated genome editing. The gRNAs were subcloned into the BsaI site of pMpGE_En04. The resultant constructs were transferred to the pMpGE010 vector by the Gateway LR reaction as described previously ().
Plant transformation
Transformation of M. polymorpha (Tak-1 accession) was performed via the AgarTrap method as described previously (). Transformants were selected on the medium supplemented with 100 μg ml−1 cefotaxime and either 10 μg ml−1 hygromycin or 0.5 μM chlorsulfuron depending on the selection marker genes in the binary vectors and cultivated for about 1 month to obtain G1 gemmae ().
Bioluminescence monitoring
Gemmalings were grown on the medium at 22°C under constant light conditions for 7-10 days. Then, the plants were transferred to 12L12D conditions. For experiments examining the effect of AMI-331 (Tokyo Chemical Industry, product No. A3352) treatment on the circadian period, the plants were transferred to the medium containing various concentration of AMI-331 at the same time. 1-2 days after the transfer, plants were sprayed with a 1 mM solution of D-Luciferin (Biosynth) prepared in 0.005% (v/v) Triton X-100 (Sigma-Aldrich). After culturing the plants in 12L12D conditions for additional 2 days, bioluminescence monitoring was performed as described previously (). A time-series analysis was performed using R 3.5.0 (https://www.r-project.org/). The free-running priods (FRPs) and relative amplitude errors (RAEs) were estimated using the fast Fourier transform nonlinear least squares (FFT-NLLS) method as described previously ().
Results
M. polymorpha is an obligate long-day plant and requires multiple long days
It has long been known that reproductive transition in M. polymorpha requires LD conditions (), and recent studies demonstrated the importance of far-red light, which is abundant in sunlight but is insufficient in fluorescent lamps, in daylength response (Chiyoda et al., 2008; ; ). However, physiological details and molecular basis of day-length response remain to be explored.
To examine whether M. polymorpha has a critical daylength for reproductive transition, WT plants of a widely used accession, Takaragaike-1 (Tak-1), were grown under various daylengths from 8-h light to 16-h light in 24-h light/dark cycles (i. e., 8L16D to 16L8D), and the number of days required for the appearance of the first visible gametangiophore primordium, a convenient indicator of reproductive transition, was examined under dissecting microscope. As previously reported, Tak-1 WT plants formed the first visible gametangiophore primordium within 14 days under 16L8D conditions, and none under 8L16D conditions after 60 days (Figure 1A; ). Under 8L16D conditions, plants remained vegetative for more than 100 days (Figure 1B, see Figure 1C for plants with gametangiophores grown under 16L8D), suggesting that M. polymorpha is an obligate LD plant, in which daylength longer than a threshold is absolutely required for the induction of growth-phase transition. Under 10L14D conditions, 56% of the plants did not form any gametangiophores after 60 days, whereas all plants formed gametangiophores under 12L12D conditions (Figure 1A), indicating that the critical daylength (defined here as the daylength at which 50% of plants undergo reproductive transition; see ) of Tak-1 WT accession is about 10 hours.
Figure 1
We next examined whether a single LD is sufficient for reproductive transition or multiple LDs are required. Arabidopsis plants of accession Col-0 requires only 1 day of LD for induction of flowering (). By contrast, Tak-1 WT plants subjected to LD (16L8D) conditions for less than 6 days did not form any gametangiophores after 60 days, 8 or more days of LD were required for the induction of gametangiophore formation (Figure 1D). These differences suggest that there is the difference in the sensitivity to LD and/or in the nature of inductive signal(s) generated under LD conditions between the two species.
Alteration of circadian rhythms did not change perception of daylength
To examine whether the circadian clock is a key component in daylength measurement of M. polymorpha, we first generated clock mutants with various circadian-rhythm phenotypes and examined their daylength response. A CRISPR/Cas9 system was used with two different gRNAs to generate mutations in MpPRR or MpTOC1 in a genetic background carrying a luciferase reporter under the control of MpPRR promoter (proMpPRR : LUC) which allows bioluminescence-based rhythm assays. As previously reported, the parental proMpPRR : LUC lines showed diurnal expression patterns under constant-light (LL) conditions for several days (Figure 2B; ). We obtained four independent Mpprrge lines and two independent Mptocge lines showing altered circadian-rhythm phenotypes (Figure 2A; Supplementary Figure 2). Two Mpprrge lines, Mpprr-1ge and Mpprr-2ge, showed short-period phenotypes under LL conditions (25.3 h versus 27.9 h in WT). The rest of four lines, Mpprr-3ge, Mpprr-4ge, Mptoc-1ge and Mptoc-2ge showed arrhythmic phenotypes under LL conditions (Figures 2B, C). We then compared the timing of gametangiophore formation between WT and these 6 clock mutants under LD (16L8D) and SD (8L16D) conditions. Interestingly, there were no significant differences in the timing of gametangiophore formation between WT and clock mutants under both daylength conditions (Figure 2D; Supplementary Figure 3). In contrast to the previous observation that phase-advanced toc1-1 mutant in Arabidopsis shows early flowering under SD conditions (), neither of the short-period mutants (Mpprr-1ge and Mpprr-2ge) formed gametangiophore under SD conditions.
Figure 2
To further investigate the role of the circadian clock in daylength measurement, we attempted to use the pharmacological approach (). Recent study showed that a chemical called AMI-331, an inhibitor of casein kinase 1 (CK1), lengthens circadian period by inhibiting the phosphorylation of TOC1 and PRR5 in Arabidopsis (; ). Because a single-copy gene (Mp6g17600) for CK1, with more than 90% identity with Arabidopsis CK1 in the deduced amino-acid sequences of the kinase domain, and the ortholog or counterpart of TOC1 and PRR5 (mentioned above) are present in M. polymorpha genome, we expected that AMI-331 is also effective in lengthening the circadian period in M. polymorpha. Bioluminescence rhythms of proMpPRR : LUC under LL conditions were monitored for plants grown on the growth medium supplemented with AMI-331 at various concentrations. Although its effect was much smaller than that observed in Arabidopsis (), AMI-331 treatment lengthened the circadian period in M. polymorpha as well (Figure 3A). The most effective concentration for lengthening the circadian period was 3.6 µM with a 2-h period lengthening (Figure 3A). Based on this observation, we treated WT plants with 3.6 µM AMI-331 and investigated its effect on the timing of gametangiophore formation under LD (16L8D) and SD (8L16D) conditions. Consistent with the results of clock mutants, lengthening circadian period by AMI-331 treatment had no significant effects on the timing of gametangiophore formation (Figure 3B; Supplementary Figure 4). These results taken together indicate that, in contrast to Arabidopsis, the circadian clock is not an essential component in daylength measurement in M. polymorpha.
Figure 3
Relative duration of light and darkness within a cycle may determine daylength perception
To further confirm that the circadian clock is not a key component in daylength measurement, we performed experiments involving non-24-h light/dark cycles of various cycle period lengths (T-cycles). In general, longer T cycles advance the timing of circadian gene expression, whereas shorter T cycles delay it (). Based on this, T-cycle experiments have been used to demonstrate the involvement of the circadian clock in daylength measurement (; for Arabidopsis). Using T cycles described earlier in the analysis with Arabidopsis (), we subjected Tak-1 WT plants to T cycles ranging from 18 to 36 h comprised of light and dark periods at the ratio of 1 to 2; T = 18 h (6L12D), 24 h (8L16D), 30 h (10L20D) and 36 h (12L24D). We then analyzed the effects of T-cycles on the timing of gametangiophore formation. WT plants did not develop gemetangiophores in >60 days under any T-cycle conditions (Figure 4A; Supplementary Figures 5A–C). This is in contrast with the case of Arabidopsis in which plants showed accelerated flowering under T-cycles longer than 24 h (i.e. 28, 30, and 32 h). Taken together with the results with clock mutants, the notion that M. polymorpha has a daylength measurement system in which circadian clock does not play an essential role is supported. Additional comparisons showed that daylength measurement in M. polymorpha does not simply rely on the duration of either light or darkness, as light/dark cycles containing the same light periods (12L12D and 12L24D) or dark periods (6L12D and 12L12D) have different effects on the induction of gametangiophore formation (Figures 1A, 4A).
Figure 4
Because all T-cycles comprised of light and dark periods in a ratio of 1 to 2 had essentially the same effects on the induction of gametangiophore formation, although the total duration of a cycle differs considerably from 18 to 36 h (Figure 4A), we hypothesized that daylength measurement in M. polymorpha is based on the relative duration of light and darkness within a cycle. To test the hypothesis, we next applied Nanda-Hamner (NH) cycles comprised of a constant light period altering with a dark period of variable lengths (). If our hypothesis is correct, increasing or decreasing the hours of darkness should either inhibit or accelerate the induction of gametangiophore formation. Here we set light periods to 8 h and exposed WT plants to the following NH-cycle conditions; NH = 24 h (8L16D), 22 h (8L14D), 20 h (8L12D), 18h (8L10D) and 16h (8L8D). Although 24-h NH cycles (i.e., normal 24-h short days) were perceived as non-inductive daylength conditions, 53% of the plants formed gametangiophores under 22-h NH cycles and days to gametangiophore formation decreased as the length of dark period were further shortened (Figure 4B; Supplementary Figures 5D, E). These observations support the hypothesis that daylength perception of M. polymorpha is based on the relative duration of light and darkness within a cycle.
Additional light/dark cycles were also tested (Figure 4C). To examine whether the same conclusion holds for cycles comprised of a constant dark period altering with a light period of various lengths, we compared the timing of gametangiophore formation between 8L16D (normal 24-h short days) and 16L16D conditions. Consistent with the observation that decrease of the proportion of dark period accelerated the induction of gametangiophore formation (Figure 4B), increase of the proportion of light period from 33% (8L16D) to 50% (16L16D) has an accelerating effect (Figures 4B, C). Notably, gametangiophore formation was induced at almost the same timing under 12L12D and 16L16D conditions (24.8 and 25.1 days, respectively), both of which have a light-to-dark ratio of 1 to 1 (Figure 4C). Lastly, we investigated whether the correlation between light/dark ratios and the timing of induction is observed under cycles of extremely long durations as well. Unexpectedly, effects of 24L24D conditions on the timing of induction were critically different from those of 12L12D and 16L16D conditions (Figure 4C; Supplementary Figures 5F–H). Under 24L24D conditions, 66% of the plants did not form any gametangiophores after 60 days (Figure 4C). This implies that the correlation between light/dark ratios and the timing of induction breaks down around a certain threshold duration. At the same time, the results raise the possibility that cycles comprised of dark periods of 24 h or longer are perceived as non-inductive conditions even when combined with sufficiently long light periods. To test this possibility, we observed the timing of gametangiophore formation under 36L24D and 48L24D conditions. Under 36L24D conditions, 40% of the plants did not form any gemetangiophores after 60 days. However, days to gametangiophore formation of the induced plants were decreased compared to 24L24D (50.5 days in 24L24D vs 22.1 days in 36L24D conditions) (Figure 4C; Supplementary Figures 5I, J). Under 48L24D conditions, all the plants formed gametangiophores at earlier dates (Figure 4C). These results indicate that increase of the proportion of light is effective to accelerate the induction of gametangiophore formation even under cycles with extremely long durations of darkness.
Discussion
In the present study, we showed that alterations of circadian rhythms by genetic and pharmacological approaches have no significant effects on the timing of growth-phase transition in an obligate long-day plant M. polymorpha (accession Tak-1). Additionally, non-24h light/dark-cycle experiments suggest that there is the correlation between light-to-dark ratios in a cycle and the timing of growth-phase transition. These results taken together indicate that circadian clock is not an essential component in the daylength measurement of M. polymorpha. This is in contrast to angiosperms in which circadian clock plays a pivotal role. It is likely that daylength measurement in M. polymorpha, instead, is dependent on the light-to-dark ratio within a cycle. Inductive signal, which is somehow diminished in the dark, may be generated during light periods through phytochrome far-red high-irradiance response (). Given that M. polymorpha (accession Tak-1) requires multiple LDs (Figure 1D), presumptive inductive signal may accumulate during repeated LDs to reach a certain threshold required for growth phase transition. Hence the balance between light and dark periods will affect whether the inductive signal accumulates and/or whether the level of the accumulated inductive signal exceeds the threshold. Search for such a signal is an important project for future study.
One potential explanation for the difference of the daylength measurement may be related to the robustness of circadian oscillations. Gene numbers and complexity have generally increased during evolution of plant circadian clocks (), and complexity of the oscillators is considered to bring robustness to environmental perturbation (). The circadian clock of M. polymorpha has fewer components than Arabidopsis and has been considered to be less robust (). showed that clock gene expression immediately adapts to 6L6D conditions after transfer of plants from 12L12D conditions, which suggests that the underlying circadian clock regulation on gene expression is masked by the effect of light/dark cycles. Due to this property, the effect of the circadian clock may be overridden by the effect of light/dark cycles in daylength measurement of M. polymorpha. Whether circadian clocks of non-angiosperm plants with smaller number of components are less robust and hence they do not play a central role on daylength measurement awaits further study.
To lengthen circadian period of M. polymorpha, we used AMI-331, a period-lengthening chemical reported in Arabidopsis (). In Arabidopsis, AMI-331 inhibits the activity of CK1-family proteins, and increases PRR5 and TOC1 protein amounts (; ). In this study, we showed that AMI-331 treatment also lengthens circadian period of M. polymorpha (Figure 3A), suggesting that AMI-331 inhibits the activity of CK1 encoded by a single-copy gene (Mp6g17600), affecting MpPRR and MpTOC1 protein levels, and therby resulting in lengthening of circadian period. Further analysis of the role of CK1 in the M. polymorpha circadian clock could provide a clue to understand the structure of the M. polymorpha circadian oscillator and the evolution of the land plant circadian clocks.
In addition to contrasting difference in the involvement of the circadian clock in daylength measurement, Arabidopsis and M. polymorpha differ in their sensitivity to daylength. Arabidopsis (accession Col-0) requires only 1 day of LD for reproductive transition, whereas M. polymorpha (accession Tak-1) requires 8 or more LDs. Similar to M. polymorpha, an SD plant Chrysanthemum morifolium requires repeated SDs for inflorescence development (). In Chrysanthemum, CsFTL3 (FLOWERING LOCUS T-like 3) expression gradually increases during repeated SDs, and inflorescence development is induced when CsFTL3 signal reaches the threshold (Nakano et al., 2013). Recently, it was shown that CsFLT3 expression is positively regulated during SDs by a feedback mechanism involving the complex of CsFTL3 and its partner CsFDL1 (FD-like1) (Nakano et al., 2019). Exploration of genes whose expression gradually change during repeated LDs and possible epigenetic changes responsible for it will provide insights into the mechanisms of daylength measurement in M. polymorpha.
We showed that the circadian clock does not have the central role in daylength measurement of M. polymorpha. Further studies are required to understand how circadian clock-regulated GI-FKF1 module and a possible downstream factor CDF are involved in daylength response of M. polymorpha. Recent progress has uncovered key steps in sexual reproduction in M. polymorpha (; ). The earliest step, gametangium primordia initiation, is regulated by a basic helix-loop-helix (bHLH) gene BONOBO (BNB), whose expression is induced by LD, and another bHLH gene LOTUS JAPONICUS ROOTHAIRLESS LIKE (LRL) whose protein forms a heterodimer with BNB (; ). A conserved regulatory module of microRNA156/529 and squamosa promoter-binding-protein-like (SPL) is likely to be involved in daylength response of M. polymorpha (). A counterpart of angiosperm miR156, miR529c, prevents M. polymorpha from reproductive transition in non-inductive SD conditions by restricting MpSPL2 activity (). Other factors acting possibly downstream of daylength measurements have also been identified, such as MpDELLA and MpPIF () and MpKANADI (). It is likely that these factors control daylength-dependent reproductive transition through regulation of BNB expression. A recent work reports that a signaling pathway of unidentified gibberellin-related compounds is actually involved in BNB expression under LD condition (). The rapidly accumulating knowledge will help us to uncover the molecular mechanism underlying daylength measurement and subsequent pathway(s) leading to BNB expression. Further analysis at the molecular level in M. polymorpha should provide important insights into understanding evolution and diversification of daylength measurement systems among land plants.
Statements
Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Author contributions
YK: Conceptualization, Funding acquisition, Investigation, Writing – original draft. KI: Conceptualization, Funding acquisition, Investigation, Writing – review & editing. YT: Writing – review & editing, Supervision. SY: Supervision, Writing – review & editing, Funding acquisition. TA: Funding acquisition, Supervision, Writing – review & editing, Conceptualization.
Funding
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was funded by the Japan Society for the Promotion of Science (JSPS) (JP19H03244 to TA, JP18K06285 to SY, 20K15818 to KI, and 21J10782 to YK). YK was a JSPS research fellow.
Acknowledgments
We thank Professor Tokitaka Oyama, Kyoto University, for allowing us to use facilities for the bioluminescence monitoring.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2023.1275503/full#supplementary-material
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Summary
Keywords
circadian clock, daylength, growth-phase transition, Marchantia polymorpha, photoperiod, phytochrome
Citation
Kanesaka Y, Inoue K, Tomita Y, Yamaoka S and Araki T (2023) Circadian clock does not play an essential role in daylength measurement for growth-phase transition in Marchantia polymorpha. Front. Plant Sci. 14:1275503. doi: 10.3389/fpls.2023.1275503
Received
10 August 2023
Accepted
13 October 2023
Published
08 November 2023
Volume
14 - 2023
Edited by
Helena Fernández, University of Oviedo, Spain
Reviewed by
Chi-Lien Cheng, The University of Iowa, United States; Yogesh Mishra, Banaras Hindu University, India
Updates
Copyright
© 2023 Kanesaka, Inoue, Tomita, Yamaoka and Araki.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Takashi Araki, araki.takashi.3a@kyoto-u.ac.jp
†ORCID: Keisuke Inoue, https://orcid.org/0000-0002-9390-0432; Shohei Yamaoka, https://orcid.org/0000-0003-4154-9967; Takashi Araki, https://orcid.org/0000-0002-3616-1493
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