Seasonal drought shapes the relationship between stem CO 2 efflux and belowground respiration in an even-age rubber plantation on Hainan Island, China

Xinwei Guo^1,2*Bo Song³Rui Sun^1,2Guoyu LAN^1,2Bangqian Chen^1,2Chuan Yang^1,2Zhixiang WU^1,2*

• ¹Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
• ²Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou, China
• ³School of Accounting, Hainan Vocational University of Science and Technology, Haikou, Hainan Province, China

Stem CO 2 efflux (E c ) reflects the amount of photo-assimilated carbon released back into the atmosphere and plays a critical role in the carbon balance of tree and forest ecosystems. Despite previous studies indicating that a portion of stem CO 2 originates from root respiration (R root ), the seasonal dynamics of E c and its relationship with belowground respiration remain poorly understood, particularly in tropical regions characterized by distinct dry and wet seasons. To address this gap, we investigated E c in tapping and non-tapped rubber trees, along with environmental factors and physiological traits (sap flow flux density, root respiration, and leaf area index) from 2018 to 2021.Our results showed that tapping activity increased the E c of rubber trees compared to non-tapped trees, with increases ranging from 10.37% to 233.66%. However, the magnitude of this increase varied between the dry and wet seasons. Although tapping enhanced the E c , it did not alter the E c seasonal pattern. Consequently, E c in both tapped and non-tapped rubber trees displayed an overall single-peak pattern, with significantly lower values during the dry season compared to the wet season, suggesting growth phenology primarily regulates E c seasonal dynamics. Structural equation modeling revealed that root respiration (R root ), sap flow flux density (F d ), and soil moisture at 50 cm depth as the primary drivers of the E c variations during the dry season. In contrast, soil moisture at 5 cm depth and air temperature (T a ) were identified as dominant factors influencing E c in the wet season, with belowground respiration having a negligible influence. These results suggest that the relationship between E c and belowground respiration is environmentally sensitive and exhibits seasonal dependency.