REVIEW article
Front. Plant Sci.
Sec. Plant Biotechnology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1563711
This article is part of the Research TopicEngineering Future Crops Through Genome EditingView all 13 articles
Unlocking the Potential of CRISPR Tools and Databases for Precision Genome Editing
Provisionally accepted- Dayalbagh Educational Institute, Agra, India
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Recent breakthroughs in CRISPR/Cas genome editing have transformed molecular biology research and offer significant potential across biotechnology and medicine. This has created a broad spectrum of computational tools and databases that aim to optimize each phase of the genome-edited workflow, from guide RNA design and off-target prediction through screening analysis and biological validation. Here, we survey major CRISPR tools and analyze their features in the context of precision genome editing. CRISPOR and CHOPCHOP versatile platform that provides robust guide RNA design for several species, integrated off-target scoring, and intuitive genomic locus visualization. This review gives an overview of these new resources that have been developed, grouped based on their functionalities like design of guide RNA, off-target predictions, genome-wide screens, and visualizations of the data. Furthermore, we discuss new trends in database development like their integration with genome browsers and implementation of machine learning. This review thus gives a useful overview of the dynamic field of CRISPR/Cas genome editing tools. It also serves as a helpful guide for researchers looking to utilize these tools in their research.
Keywords: CRiSPR/Cas, Genome editing, computational tools, databases, Guide RNA design, Offtarget prediction
Received: 20 Jan 2025; Accepted: 30 Jun 2025.
Copyright: © 2025 SARASWAT and Ranjan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Rajiv Ranjan, Dayalbagh Educational Institute, Agra, India
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.