ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1567781
This article is part of the Research TopicEvolutionary Adaptations of Plant Genes: A Comprehensive Study of Phylogenomics, Epigenetic Changes, and Protein DynamicsView all 13 articles
Genome-wide identification and characterization of DNA methyltransferases and demethylases in Siraitia grosvenorii
Provisionally accepted
- 1State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, Beijing Municipality, China
- 2Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China
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DNA methylation and demethylation play a crucial role in plant development, fruit ripening, and the accumulation of secondary metabolites. It is primarily catalyzed and regulated by cytosine-5 DNA methyltransferases (C5-MTases) and DNA demethylases (dMTases). In our study, six C5-MTase and four dMTase genes were identified in Siraitia grosvenorii genome. Phylogenetic analysis demonstrated that the six SgC5-MTase were divided into four categories, SgMET1, SgCMTs, SgDRMs, and SgDNMT2. The four SgdMTase were grouped into SgROS1, SgDML3, SgDME subfamilies. Transcript abundance levels of SgC5-MTase and SgdMTase genes revealed changes during vegetative and reproductive development. Furthermore, the expression of SgdMTase genes was upregulated during fruit ripening, while SgCMT2/3 genes were downregulated. This indicates a potential rise in demethylation, aligning with the accumulation pattern of mogroside V. Our results suggest a role for DNA methylation modifications in the growth, development, maturation, and accumulation of mogrosides, which will also facilitate future epigenetic studies in S. grosvenorii.
Keywords: Siraitia grosvenorii, C5-MTase, dMTase, Fruits, Gene Expression
Received: 28 Jan 2025; Accepted: 03 Oct 2025.
Copyright: © 2025 Zang, Wang, Su, Xie, Mo, Luo and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zuliang Luo, zuliangluo@163.com
Xiaojun Ma, xjma@implad.ac.cn
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