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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Plant Pathogen Interactions

Positional cloning of a peanut CC-NBS-LRR gene, AhRRS6, confers resistance to Ralstonia solanacearum

Provisionally accepted
Huiwen FuHuiwen Fu1Yuhui ZhuangYuhui Zhuang2Chong ZhangChong Zhang3Shipeng LiShipeng Li2Yongli ZhangYongli Zhang2Wenzhi LuWenzhi Lu2Lihui WangLihui Wang2Sheidu AbdullazizSheidu Abdullaziz4Yuting ChenYuting Chen2Tiecheng CaiTiecheng Cai2Qiang YangQiang Yang2XiangYu ChenXiangYu Chen2Rajeev K VarshneyRajeev K Varshney5Zujian WuZujian Wu6Hua ChenHua Chen2*Weijian ZhuangWeijian Zhuang3*
  • 1College of Plant Protection&Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute&State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, China
  • 2College of Life Science&Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
  • 3Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute, Fujian Agriculture and Forestry University, Fuzhou, China
  • 4College of Life Science&Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute&Department of Agronomy, Faculty of Agriculture, Nasarawa State University, Keffi, P.M.B 1022, Keffi Nasarawa State, Nigeria, Fujian Agriculture and Forestry University, Fuzhou, China
  • 5Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute&Centre for Crop and Food Innovation, State Agricultural Biotechnology Centre, Food Futures Institute, Murdoch University, Murdoch 6150, Australia., Fujian Agriculture and Forestry University, Fuzhou, China
  • 6State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou, China

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Bacterial wilt, caused by Ralstonia solanacearum, is a destructive disease with no effective chemical control, severely affecting global crop production. This study applied BSR-seq on 581 recombinant inbred lines (RILs), combined with linkage mapping, to identify resistance quantitative trait loci (QTL). Illumina sequencing yielded 189.6 Gb of data, identifying 70,035 high-quality SNPs from 55,840 genes. Two resistance loci were mapped on chromosome 12: a novel 1.11 Mb QTL and an adjacent 1.03 Mb region.Five CC-NBS-LRR-type resistance candidate genes were identified. The AhRRS6 alleles were cloned, and three allele-specific SNP markers were developed and validated across peanut breeding varieties. Additionally, 3,851 differentially expressed genes were detected, including key resistance-related genes.Transgenic AhRRS6y conferred strong resistance to R. solanacearum, while AhRRS6x caused susceptibility in both Nicotiana benthamiana and Arabidopsis thaliana. These alleles differentially regulated genes in HR, ETI, and PTI pathways, particularly affecting NbPDF1.2 and NbNDR1. AhRRS6y expression reduced oxidative damage, indicated by lower malondialdehyde and higher ascorbate peroxidase activity. This work provides critical genetic resources for breeding bacterial wilt-resistant peanut varieties and enhances the mechanistic understanding of plant immune responses.

Keywords: AhRRS6, Bacterial wilt resistance, BSR-seq, functional characterization, peanut, Ralstonia salanacearum

Received: 03 Oct 2025; Accepted: 19 Dec 2025.

Copyright: © 2025 Fu, Zhuang, Zhang, Li, Zhang, Lu, Wang, Abdullaziz, Chen, Cai, Yang, Chen, Varshney, Wu, Chen and Zhuang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Hua Chen
Weijian Zhuang

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material