Iron-Mediated Remediation of Arsenic-Induced Suppression of Root Morphogenesis and Radial Oxygen Loss in Wetland Plant

Sitong Jiang¹Zhengyu Zhu¹Yitong Pan²Rongrong Shi³Zhixi Wang³Mohammad Mazbah Uddin⁴Song Wang¹Jingchun Liu³Kang Mei^1*

• ¹Jiangsu Ocean Universiity, Lianyungang, China
• ²Princeton University, Princeton, United States
• ³Xiamen University, Xiamen, China
• ⁴Peking University, Beijing, China

Arsenic (As) contamination in paddy soils disrupts root morphogenesis and radial oxygen loss (ROL), yet the dynamic interplay between iron plaque formation and plant physiological adaptation remains poorly understood across temporal scales. This study investigated iron (Fe)- mediated mitigation of inorganic As toxicity through hydroponic experiments with varying As (0, 10 and 30 μmol/L) and Fe (0–100 μmol/L) concentrations over exposure periods ranging from acute to chronic exposure scenarios. Pot experiments revealed that 1-week high-As (HAs) exposure reduced chlorophyll content by 28% and suppressed ROL by 50% compared with the control group (CK), whereas prolonged low-As (LAs) exposure induced partial chlorophyll recovery (LAs > CK > HAs). Fe supplementation enhanced root biomass by 1.18–2.39 times under chronic As stress and significantly increased ROL rates by 1.38–2.83 times through Fe-plaque formation, which sequestered 38–52% of root As. Root porosity peaked at 9.84% under As-Fe co-treatment but showed an inverse correlation with ROL. Anatomical analysis revealed Fe-driven restoration of aerenchyma (46.32% cross-sectional area) and reinforcement of lignification barriers. These findings highlight Fe's dual role in alleviating As toxicity through physical immobilization and physiological adaptation, offering insights into iron-based remediation strategies for As-contaminated wetlands.