ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Physiology
This article is part of the Research TopicInduced Resistance for a Sustainable Future of AgricultureView all 4 articles
Multi-omics analysis of molecular mechanisms driving the grafting-enhanced resistance of tea plants to Colletotrichum camelliae
Provisionally accepted
- 1Guizhou Tea Research Institute, Guizhou Province Academy of Agricultural Science, Guiyang, China
- 2Guizhou University, Guiyang, China
- 3Guizhou Academy of Agricultural Sciences, Guiyang, China
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Background: The fungal pathogen Colletotrichum camelliae causes a devastating disease that severely limits tea plant (Camellia sinensis) yield and quality. Grafting onto resistant rootstocks offers a sustainable control strategy, yet resistant rootstocks confer scion protection remains obscure. Methods: Susceptible cultivar 'Qianmei 818' was grafted onto resistant 'Qianmei 419'. Profiled systemic defenses using integrated RNA-seq, sRNA-seq, and metabolomics, complemented by phytohormone and defense-enzyme assays and qRT-PCR validation. Results: Hetero-grafting conferred near-complete resistance, reducing lesion diameters by 98.71% compared to ungrafted controls, with elevated PAL activity and accumulation of IAA, GA3, and MeSA. Multi-omics analyses identified 1205 differentially expressed genes, 157 differentially expressed miRNAs, and 791 differential metabolites. Pathway integration indicated extensive reprogramming of phenylpropanoid biosynthesis, sulfur metabolism, and plant hormone signaling. Notably, specific miRNA-mRNA regulatory modules, such as downregulation of csi-miR395b-3p and a novel miR397 paralleled up-regulation of their targets in sulfur assimilation (CsAPS1) and lignin biosynthesis (CsCCoAOMT, CsCCR2), respectively, linking miRNA control to reinforcement of structural and biochemical defenses. Conclusions: Resistant rootstocks activate scion-wide defense networks through miRNA-mediated transcriptome-metabolome remodeling, achieving robust resistance while maintaining tea quality. The elucidated modules provide actionable targets and genetic resources for breeding and grafting strategies toward sustainable disease management.
Keywords: Camellia sinensis, Colletotrichum camelliae, Disease Resistance, Grafting, multi-omics
Received: 20 Nov 2025; Accepted: 22 Dec 2025.
Copyright: © 2025 Li, Lin, Wang, Wei, Yan and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Donghai Yan
De-gang Zhao
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