Regulatory networks and molecular mechanisms underlying salt stress tolerance in rice

Wuyun Fang¹Ali Raza²Qian Zhu¹Wang Qiming¹Ren Qun³Liu Mengyang³Shimei Wang^1*Muhammad Ahmad Hassan^4*

• ¹Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China
• ²School of Plant Protection, Anhui Agricultural University, Hefei, China
• ³College of Agriculture, Anhui Science and Technology University, Bengbu, China
• ⁴Anhui Agriculture University School of Resources and Environment, Hefei, China

Salinity and alkaline stress severely restrict rice productivity by disrupting ionic balance, generating oxidative damage, and impairing growth across developmental stages. Despite the significant advances in the salt tolerance knowledge, rice is very sensitive in contrast to other cereals, which demonstrates gaps in mechanistic understanding and breeding efficiency. This review incorporates the progress in the salt perception, signaling, and stress adaptation, and introduces limitations that slow down the practical improvement. Rice senses salinity using receptor-like kinases and Ca2+-dependent signaling pathway but the initial stages of the response and down-stream phosphorylation cascades have not been characterized well. The reactive oxygen species (ROS) triggered by salinity activate antioxidant mechanisms like AsA-GSH, but it is still not clear how they are spatially and organelle-specifically controlled. Proteomic analyses show extensive reorganization of proteins in signaling, cytoskeleton dynamics, metabolism and protein turnover, but most of the identified candidates have not been validated functionally. Na+ exclusion, vacuolar sequestration, and K+ retention through HKTs, NHXs, and V-ATPases are involved in ion homeostasis, but the interactions between them in tissues have not been fully understood yet. QTL studies have also reported important loci like Saltol and qSKC1 but there are slow advances made in using them in elite cultivars. New multi-omics techniques and CRISPR-based genome editing are currently providing a chance to uncover knowledge gaps. All in all, this review presents an overall framework to develop mechanistic knowledge and speed up breeding salt-resistant varieties of rice.