Multi-trait and Multi-environment Genomic Prediction Enhances Yield Components Improvement in Durum Wheat

Damiano Puglisi¹José Crossa²Jaime Cuevas³Fabio Fania¹Paolo Vitale^4*Pasquale De Vita^1*

• ¹Research Centre for Cereal and Industrial Crops, Council for Agricultural and Economics Research (CREA), Foggia, Italy
• ²Colegio de Postgraduados, Campus Montecillo, Carretera México-Texcoco, Montecillo, Texcoco, Estado de México, Mexico
• ³Universidad de Quintana Roo, Chetumal, Mexico
• ⁴Centro Internacional de Mejoramiento de Maiz y Trigo, Texcoco, Mexico

Durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn.] is a staple crop for the pasta and semolina industries, particularly in Mediterranean and semi-arid regions where climate variability poses major challenges to yield stability. This study evaluates the performance of single-environment (SE), multi-trait (MT), multi-environment (ME), and multi-trait– multi-environment (MTME) genomic prediction models across seven key traits, such as grain number per spike, grain weight per spike, number of spikelets per spike, spike length, spike weight, heading date, and plant height. Using genomic (G) and target gene-based (G2) relationship matrices with two cross-validation scenarios (CV1 and CV2), MTME models achieved the highest prediction accuracies, particularly under CV2 and sowing-by-season grouping. Modeling G2 information improved predictions for morpho-phenological traits (i.e. heading date and plant height), confirming the utility of functional allele data for capturing gene effects. MTME models effectively leveraged inter-trait and inter-environment covariance, providing biologically realistic predictions of genotype performance across simulated Mediterranean environments. These findings establish MTME genomic prediction as a powerful and scalable framework for climate-resilient durum wheat improvement, supporting predictive and data-driven breeding pipelines aimed at enhancing genetic gain and stability across years and environments.