ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Photosynthesis and Photobiology
Effects of saturation flash protocols on photosynthetic induction and assessment of mesophyll conductance and maximum carboxylation rate sensitivity to parameter inputs in Ficus carica
Provisionally accepted
- 1Zhoukou Normal University, Zhoukou, China
- 2Beijing Chest Hospital Affiliated to Capital Medical University, Beijing, China
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Improving photosynthetic efficiency during the induction phase is a key strategy for enhancing plant productivity. A critical prerequisite for achieving this goal is identifying the limitation factors that affect photosynthesis during induction. While stomatal conductance to water vapor (gsw) can be quantified using gas exchange measurements, the quantification of mesophyll conductance (gm) and maximum carboxylation rate (Vcmax) remains uncertain and underexplored. This study, using Ficus carica as plant material, aims to investigate whether saturation flashes influence the photosynthetic induction process and assess the sensitivity of gm, Vcmax, and photosynthetic limitation analysis to key photosynthetic parameters. The results revealed no significant differences in the dynamics of photosynthesis and stomatal conductance between treatments with and without saturation flashes, indicating that saturation flashes did not interfere with the photosynthetic induction process. Further analysis of the photosynthetic induction process showed that gm and Vcmax exhibited a gradual increase during induction. Moreover, sensitivity analysis demonstrated that variations in the leaf absorptance parameter (αβ) and CO₂ compensation point (Γ*) significantly impacted the estimation of gm and Vcmax, while changes in the Rubisco affinity constant (Km) also notably influenced Vcmax. Finally, photosynthetic limitation analysis revealed that stomatal limitation (SL) was the primary factor limiting photosynthesis in Ficus carica, accounting for 41.4% of the total limitation, followed by mesophyll conductance limitation (MCL) and biochemical limitation (BL), which accounted for 30.9% and 27.7%, respectively. Statistical analysis revealed no significant changes in the overall pattern of photosynthetic limitations across different sensitivity analysis scenarios. Overall, this study demonstrates that saturation flashes do not interfere with photosynthetic induction in Ficus carica, while changes in αβ, Γ*, and Km affect the estimation of gm and Vcmax but have no significant impact on the estimation of photosynthetic limitations. This study provides a theoretical basis for improving photosynthetic efficiency during the induction phase in Ficus carica and enhances our understanding of photosynthetic induction measurement and calculation methods.
Keywords: Biochemical capacity, Chlorophyll Fluorescence, mesophyll conductance, Photosynthetic induction, Photosynthetic limitations
Received: 04 Dec 2025; Accepted: 06 Feb 2026.
Copyright: © 2026 Jin, Li and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Guo-Yin Li
Jinping Wang
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