ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Biotechnology
Establishment of an Industrialized Micro-propagation System for Cerasus campanulata Maxim
Provisionally accepted
- 1Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- 2Ningbo City College of Vocational Technology, Ningbo, China
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Cerasus campanulata Maxim. possesses high ornamental value owing to its early flowering period, holding considerable application potential. However, research on its propagation techniques is still in the preliminary stage. To establish an efficient regeneration system for C. campanulata, this study used tissue-cultured seedlings as explants and employed an orthogonal experimental design to investigate the effects of key factors at different culture stages on plant regeneration. The results indicated that the suitable proliferation medium was ½MS supplemented with 0.1 mg/L NAA, 0.5 mg/L 6-BA, and 2.5 g/L banana powder. With this medium, the survival rate reached 100%, the proliferation rate was 96.67%, the proliferation coefficient exceeded 4, and the average bud height was 4.57 cm. Furthermore, the regenerated buds exhibited vigorous and healthy growth. The suitable rooting medium was ½MS, which yielded a rooting rate of over 88% and a survival rate of over 88% after greenhouse acclimatization. After 120 days of pot cultivation, the regenerated plants achieved an average plant height of 57.83 cm, an average leaf length of 15.03 cm, an average leaf width of 6.31 cm, and an average of 17 leaves per plant. Genetic stability analysis of the regenerated plantlets after three subculture cycles showed no obvious genetic variation. In conclusion, this study successfully established a practical regeneration system for C. campanulata, covering proliferation, rooting, acclimatization, pot cultivation, and preliminary genetic stability verification, which provides a reliable technical basis for the large-scale propagation and application of this species.
Keywords: Cerasus campanulata Maxim., In vitro Propagation, ISSR, Medium optimization, orthogonaldesign, woody plants
Received: 12 Dec 2025; Accepted: 03 Feb 2026.
Copyright: © 2026 Ding, He, Lv, Mou, Yu, Wang, Chen and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhi Chen
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