Abstract
Arabidopsis flotillin 2 (At5g25260) belongs to the group of plant flotillins, which are not well characterized. In contrast, metazoan flotillins are well known as plasma membrane proteins associated with membrane microdomains that act as a signaling hub. The similarity of plant and metazoan flotillins, whose functions most likely consist of affecting other proteins via protein–protein interactions, determines the necessity of detecting their interacting partners in plants. Nevertheless, identifying the proteins that form complexes on the plasma membrane is a challenging task due to their low abundance and hydrophobic character. Here we present an approach for mapping Arabidopsis thaliana flotillin 2 plasma membrane interactors, based on the immunoaffinity purification of crosslinked and enriched plasma membrane proteins with mass spectrometry detection. Using this approach, 61 proteins were enriched in the AtFlot-GFP plasma membrane fraction, and 19 of them were proposed to be flotillin 2 interaction partners. Among our proposed partners of Flot2, proteins playing a role in the plant response to various biotic and abiotic stresses were detected. Additionally, the use of the split-ubiquitin yeast system helped us to confirm that plasma-membrane ATPase 1, early-responsive to dehydration stress protein 4, syntaxin-71, harpin-induced protein-like 3, hypersensitive-induced response protein 2 and two aquaporin isoforms interact with flotillin 2 directly. Based on the results of our study and the reported properties of Flot2 interactors, we propose that Flot2 complexes may be involved in plant–pathogen interactions, water transport and intracellular trafficking.
Introduction
The SPFH (stomatin/prohibitin/flotillin/HflK/C) domain proteins superfamily consists of membrane proteins which exhibit 40–84% sequence homology (Li et al., 2000; Green and Young, 2008), but are divided into several groups with different functions and localizations (Browman et al., 2007). Flotillins form a group of SPFH domain-containing proteins characterized by their localization in the plasma membrane.
Flotillins were discovered in three independent studies as a human epidermal surface antigen (Schroeder et al., 1994), as proteins induced during optic nerve regeneration in the goldfish retinal ganglion (Schulte et al., 1995) and as proteins of membrane caveolae in a mouse fibroblast tissue culture (Bickel et al., 1997). To this day, the localization and function of metazoan flotillins has been intensively investigated. Metazoan flotillins are predominantly targeted to plasma membrane microdomains, where they are anchored by the SPFH domain (Morrow et al., 2002; Neumann-Giesen et al., 2004; Glebov et al., 2006; Solis et al., 2007; Langhorst et al., 2008). They were found to be involved in the endocytosis of glycophosphatidylinositol (GPI)-anchored proteins as well as caveolae-mediated endocytosis (Volonté et al., 1999; Baumann et al., 2000). Another function of flotillins likely consists of affecting other proteins via protein–protein interactions (Baumann et al., 2000), in which case various tyrosine kinases (Ullrich and Schlessinger, 1990; Neumann-Giesen et al., 2007; Amaddii et al., 2012) or proteins of the cytoskeleton (Baumann et al., 2000; Liu et al., 2005; Langhorst et al., 2008; Peremyslov et al., 2013) are prominent interactors with mammalian flotillins. The interaction with partner proteins as well as homo- and hetero-oligomerization of single metazoan flotillin isoforms is predominantly provided by the C-terminal domain of flotillins, where several coiled-coil stretches are present (Neumann-Giesen et al., 2004; Solis et al., 2007).
The coding regions of flotillin homologs were also identified in various plant genomes (Di et al., 2010). For example, the A. thaliana genome contains three homologs of flotillin, Flot1 (At5g25250), Flot2 (At5g25260), and Flot3 (At5g64870) (Gehl et al., 2014; Jarsch et al., 2014) and in this paper these three isoforms are designated Flot1/2/3 unless stated otherwise. Similarly to metazoan homologs, Arabidopsis flotillins are able to form heterooligomers via their C-terminal domain, which was reported for the direct interaction of Flot1 with Flot3 (Yu et al., 2017). However, the role of plant flotillins, as well as of most other proteins with a SPFH domain, has not been fully elucidated. Current findings about the localization and function of plant flotillins in the context of the known role of metazoan flotillins have been recently summarized by Danek et al. (2016). Similarities between the properties of plant and metazoan flotillins lead to the assumption that plant flotillins affect other proteins via protein–protein interactions, as with metazoans.
Arabidopsis thaliana flotillins differ in the localization of their transcription, because Flot1 and Flot2 are predominantly transcribed in leaves and shoots, while Flot3 is mostly transcribed in the flower parts and siliques (Danek et al., 2016). Nevertheless, the subcellular localization is similar for all known flotillins; they are most frequently localized to plasma membrane microdomains (Li et al., 2012; Hao et al., 2014; Jarsch et al., 2014; Ishikawa et al., 2015), which are enriched in sterols, sphingolipids, saturated phospholipids and GPI-anchored proteins, and play a significant role in membrane trafficking and cell signaling (Simons and Ikonen, 1997; Simons and Toomre, 2000; Borner et al., 2005; Jarsch et al., 2014; Cacas et al., 2016).
Although the anchoring of mammalian flotillins is supported by their palmitoylation as well as myristoylation (Morrow et al., 2002; Neumann-Giesen et al., 2004; Langhorst et al., 2008), no sites for palmitoylation or myristoylation were predicted in any of the three A. thaliana flotillins. This indicates that the anchoring to the membrane is provided by a different mechanism (Danek et al., 2016). This mechanism could be based on the specific interaction with sterols, since several putative CRAC/CARC motifs providing recognition and interaction with sterols were predicted in the sequence of plant flotillins (Roitbak et al., 2005; Danek et al., 2016). This hypothesis is supported by the finding that the Flot1 diffusion coefficient is decreased in plants treated with methyl-β-cyclodextrin, a sterol-depleting agent (Li et al., 2011, 2012; Hao et al., 2014). Moreover, it was also observed that the knocking-down of Flot1 affected the internalization of sterol into membranes (Li et al., 2012).
Since proteins involved in vesicular trafficking and endocytosis (e.g., ESCRT proteins, exocyst and SNARE subunits or Rab-GTPase) were proposed to be Flot2 and Flot3 interactors by Associomics, a split-ubiquitin yeast system-based database of direct protein–protein interactions1 (Jones et al., 2014), this suggests that plant flotillins could play a similar role in membrane transport to mammalian ones. Additionally, plant flotillin microdomains have been shown to be involved in clathrin-independent endocytosis, inducible by various stimuli (Li et al., 2011; Hao et al., 2014; Wang et al., 2015; Yu et al., 2017). The role of flotillins in cell communication and signal transduction is also considered, because several types of kinases were found to co-localize with Medicago truncatula Flot4 (Haney et al., 2011) and interact with all three AtFlot isoforms (Associomics). The involvement of flotillins in plant–pathogen interactions was demonstrated, as Flot1 lateral mobility in the plasma membrane was altered upon treatment with bacterial elicitor flg22, and reduced or increased flg22-induced callose deposition was observed in plants with Flot1 knocked-down or overexpressed, respectively (Yu et al., 2017). Arabidopsis amiRNA-Line with reduced Flot1/Flot2 expression were smaller in size and exhibited structural changes in apical meristems (Li et al., 2012), which points to the involvement of flotillins in plant growth and development. Moreover, functional linkage and co-localization of plant flotillins and the cytoskeleton was observed (Li et al., 2012; Peremyslov et al., 2013).
Protein interactions with other cell components are crucial to maintaining the viability of the whole organism and determining its phenotypic manifestation. Predominantly, protein–protein interactions are nowadays intensively examined by various methods. Among them, immunoprecipitation (IP) or affinity purification (AP) coupled to mass spectrometry (MS) is the method of choice (ten Have et al., 2011; Dunham et al., 2012; Dedecker et al., 2015). However, the investigation of membrane proteins is challenging due to their hydrophobic character and low abundance. Nevertheless, there is a current effort to modify standard procedures in order to facilitate analyses of membrane protein interactions (Qi and Katagiri, 2009; Smaczniak et al., 2012; Van Leene et al., 2015).
The aim of this study was to perform a screening of Flot2 protein interactors in A. thaliana leaves. For this purpose we used IP with a GFP tag followed by the MS of in vitro cross-linked membrane proteins. After we confirmed the localization of Flot2 at the plasma membrane, we showed that the enrichment of the plasma membrane prior to the IP-MS is a crucial step for the detection of low–abundance plasma membrane interactors. The direct interaction of Flot2 with several proteins involved in the plant response to biotic as well as abiotic stress was confirmed by an independent method, e.g., by a split-ubiquitin yeast system (SUS) suitable for the analysis of membrane proteins.
Materials and Methods
Plant Material
Transgenic A. thaliana lines AtFlot2-GFP with the p35S::AtFlot2:GFP construct that stably produces the Flot2-GFP protein were prepared as follows: The coding sequence was amplified from cDNA prepared from Col-0 using specific primers 1 and 2 (see Supplementary Table S1) and in-frame introduced in between the EcoRI and BamHI sites of a modified pGreen0029 vector containing the CAMV 35S promoter and 3′-terminal GFP coding sequence by restriction/ligation. Stable transformants were obtained by the Agrobacterium tumefaciens floral dip method and selected on kanamycin plates. T3 generation plants were used for microscopy and membrane fractions preparation.
Seeds of the A. thaliana wild type (WT, ecotype Col-0) and AtFlot2-GFP plants were stratified for 3 days at 4°C, placed on Jiffy 7 peat pellets and cultivated in a growth chamber at 22°C, with a 10-h day (100–130 μmol m-2 s-1) and 14-h night cycle at 70% relative humidity for 1 week. One-week-old plantlets were individually replanted to Jiffy 7 peat pellets and placed in a cultivation room with a 16-h day (100–130 μmol m-2 s-1) and 8-h night cycle and 40–50% relative humidity. During the cultivation, plants were watered with distilled water. Whole rosettes of 4-week-old plants were frozen in liquid nitrogen to be used as the material for MS analyses.
Confocal Microscopy
For microscopic observations, seeds of AtFlot2-GFP plants were surface sterilized and sown onto Murashige-Skoog basal salt (Duchefa) 1% agar plates supplemented with 1% sucrose. The seedlings were grown in a vertical position under 100 μmol m-2 s-1 in a 16/8 h and 22/20°C (light/dark) cycle. Five-day-old seedlings were observed using a Zeiss 880 laser scanning confocal microscope. Plasmolysis was induced by treatment with 0.8 M mannitol in Murashige-Skoog solution for 30 min. Subsequently, the seedlings were incubated in propidium iodide solution (20 μg/ml in Murashige-Skoog + 0.8 M mannitol) to counterstain the cell walls. GFP fluorescence was collected in the 500–550 nm range using 488 nm laser excitation and a 40× water immersion objective (NA = 1.2).
Preparation of Microsomal and Plasma Membrane Fractions
Membrane fractions were prepared from 30 g of leaves from 4-week-old A. thaliana WT and AtFlot2-GFP plants. Leaves were ground with a pestle and mortar in liquid nitrogen and further homogenized by sonication for 3 × 35 s (25 W) in 90 ml of extraction buffer (50 mM HEPES pH 7.5, 400 mM sucrose, 85 mM KCl, 100 mM MgCl2.6H2O, 0.02 mM ascorbic acid) containing cOmpleteTM EDTA-free Protease Inhibitor Cocktail according to the manufacturer’s instructions (Sigma Aldrich). The homogenate was centrifuged at 5000 ×g for 20 min at 4°C. The supernatant was filtered through Miracloth (Millipore) and centrifuged at 200,000 ×g for 1 h at 4°C. The pellet (microsomal membrane fraction) was resuspended in resuspension buffer (20 mM HEPES pH 7.5, 330 mM sucrose, 1 mM EDTA) to a total volume of 6 ml, further homogenized in a Potter-Elvehjem homogenizer and cross-linked by the addition of dithiobis (succinimidyl propionate) (DSP, Thermo Scientific) to a final concentration of 5 mM. The suspension was incubated for 30 min at 4°C with shaking. To quench the reaction, 1 M Tris (pH 7.5) was added to a final concentration of 50 mM, and the suspension was shaken again for 30 min at 4°C. The microsomal fraction with cross-linked proteins was pelleted by centrifugation at 200,000 ×g for 1 h at 4°C.
To release the protein complexes from the microsomal fraction, the pellet was resuspended in 6 ml of resuspension buffer and homogenized with a Potter-Elvehjem homogenizer, and then 10% (w/v) sodium deoxycholate was added to a final concentration of 0.5% (w/v). The suspension was incubated for 30 min at 4°C. Solubilized proteins were collected in the supernatant obtained by centrifugation at 200,000 ×g for 30 min at 4°C, and the pellet was resuspended in the same way as before. To further enrich the plasma membrane, the pellet of the cross-linked microsomal fraction was resuspended in 5 mM K/Na-phosphate buffer (pH 7.8) and homogenized with a Potter-Elvehjem homogenizer.
The plasma membrane fraction was prepared from the cross-linked membrane fraction with a PEG/dextran two-phase system (Schindler and Nothwang, 2006; Pleskot et al., 2010). After gentle mixing, the separation was carried out overnight at 4°C, and the tubes were centrifuged at 1500 ×g for 5 min at 4°C. The upper phase containing the plasma membrane was transferred to the new tubes, mixed with a blank lower phase and centrifuged again. The final upper phase was collected, diluted with three volumes of 5 mM K/Na-phosphate buffer (pH 7.8) and centrifuged again at 200,000 ×g for 1 h at 4°C. The pellet was resuspended in 600 μl of resuspension buffer, 10% (w/v) sodium deoxycholate was added to a final concentration of 0.5% (w/v), and the suspension was incubated for 30 min at 4°C. The protein content in all isolated fractions was determined by Popov’s method (Popov et al., 1975) using bovine serum albumin as the standard. Flow chart of the procedure is depicted on Figure 2A.
Western Blotting and Immunodetection
The content of Flot2-GFP in the respective fractions was investigated by western blotting and immunodetection. Proteins were separated in 10% polyacrylamide SDS-gels at 180 V and electroblotted onto nitrocellulose membranes (BioTraceTM NT Nitrocellulose Transfer Membrane, Pall Corporation) at 50 V. Membranes were rinsed in PBS and blocked in 5% (w/v) non-fat milk powder in PBS with 0.075% (w/v) Tween-20 (PBST-75) overnight. Blocked membranes were washed three times in PBST-75 and incubated with primary antibodies diluted in 5% (w/v) non-fat milk powder in PBST-75 for 1 h. Anti-GFP rabbit polyclonal serum (Thermo Scientific) 1:5000 was used as the primary antibody. Membranes were washed three times in PBST-75 and incubated for 1 h with the secondary antibody, GAR/IgG(H + L)/PO (Nordic-MUbio) 1:5000 diluted in 5% (w/v) non-fat milk powder in PBST-75. Signals were visualized with an AEC staining kit (Sigma Aldrich) or ClarityTM Western ECL Substrate (Bio-rad).
Immunoprecipitation of Microsomal and Plasma Membrane Fractions
The IP procedure was performed with Dynabeads® Protein A microbeads (Thermo Scientific) with bound anti-GFP mouse monoclonal antibody, isotype IgG2a (Thermo Scientific). Fifty microliter of pre-washed beads were mixed with 2 μg of antibodies dissolved in PBS containing 0.05% Tween-20 (PBST-5) and incubated for 1 h in the vertical rotator. Beads with bound antibodies were washed three times with 200 μl of PBST-5 and incubated in 5 mM bis(sulfosuccinimidyl)suberate (BS3, Thermo Scientific) for 30 min to cross-link the bound antibodies to protein A. The reaction was quenched by washing the beads with 200 μl of 1 M Tris/HCl (pH 7.4) three times. The beads were then equilibrated three times with 200 μl of resuspension buffer with 0.5% (w/v) sodium deoxycholate and incubated with 200 μl of the respective membrane fraction for 2 h in a vertical rotator. Protein complexes bound to the beads were washed three times with RIPA buffer (50 mM Tris/HCl pH 7.4, 1 mM EDTA, 50 mM NaCl, 0.5% (w/v) sodium deoxycholate, 1% (w/v) NP-40) and eluted by incubation of the beads with 20 μl of Laemmli buffer 2× for 10 min at 95°C.
Tryptic Digestion of Proteins
Proteins eluted from the microbeads were separated to a distance of 1.5 cm in 10% poly-acrylamide SDS-gels at 180 V. Gels were stained with ImperialTM Protein Stain (Thermo Scientific) and whole line of each elute of was collected. Each lane was further sliced into smaller gel pieces and combined into an Eppendorf tube, washed with water, destained with 0.1 M NH4HCO3/acetonitrile 1:1 (v/v) and dried with acetonitrile. To reduce and alkylate the disulphide bonds, the gel pieces were first incubated with a 10 mM solution of dithiothreitol in 0.1 M NH4HCO3 for 45 min at 56°C, and then in a 55 mM solution of iodoacetamide in 0.1 M NH4HCO3 for 30 min at room temperature. Iodoacetamide solution was discarded and the gel pieces were washed with 0.1 M NH4HCO3/acetonitrile 1:1 (v/v) for 10 min and dried with acetonitrile. MS-Grade Trypsin solution at a concentration of 12.5 μg ml-1 dissolved in cold 50 mM NH4HCO3 was added to the gel pieces in a volume equal to the volume of the pieces, and the mixture was incubated on ice for 30 min. The excess trypsin solution was then discarded; the pieces were covered with 50 mM NH4HCO3 and incubated overnight at 37°C. The peptides were extracted from the gel by two consecutive sonications in 35 and 70% solutions of acetonitrile in 0.1% trifluoroacetic acid. Both aliquots were combined and the resulting peptide solution was lyophilized. The lyophilizate was then resuspended in 0.1% trifluoroacetic acid, desalted with ZipTip pipette tips according to the manufacturer’s instructions (Millipore) and purified samples were dried in air.
LC-MS/MS Analysis
The mass spectrometric analysis was performed with a UHPLC Dionex Ultimate3000 RSLC nano (Dionex) coupled with an ESI-Q-TOF Maxis Impact (Bruker Daltonics) mass spectrometer. Dried samples were dissolved in a mixture of water:acetonitrile:formic acid (97:3:0.1%) and loaded into the trap column, an Acclaim PepMap 100 C18 (100 μm × 2 cm, particle size 5 μm, Dionex), with a mobile-phase flow rate of 5 μLmin-1 of A (0.1% formic acid in water) for 5 min. The peptides were then separated in the analytical column, an Acclaim PepMap RSLC C18 (75 μm × 150 mm, particle size 2 μm, Dionex), and eluted with mobile-phase B (0.1% formic acid in acetonitrile) using the following gradient: 0 min 3% B, 5 min 3% B, 95 min 35% B, 97 min 90% B, 110 min 90% B, 112 min 3% B, and 120 min 3% B. The flow rate during the gradient separation was set to 0.3 μLmin-1. Peptides were eluted directly to the ESI source-captive spray (Bruker Daltonics). Measurements were performed in DDA mode with precursor-ion selection in the range of 400–1400 Da; up to 10 precursor ions were selected for fragmentation from each MS spectrum.
Peak lists were extracted from the raw data with the software Data Analysis 4.1 (Bruker Daltonics). Proteins were identified in the software Proteinscape 3.1 (Bruker Daltonics) using in-house Mascot server 2.4.1 (Matrix Science) with the A. thaliana protein database downloaded from2 (October 2016). The parameters for the database search were set as follows: carbamidomethyl (C) as fixed modification, oxidation (M) and CAMthiopropanoyl (K, N-terminus) as variable modifications, tolerance 10 ppm in MS mode and 0.05 Da in MS/MS mode, enzyme trypsin one miscleavage. In MS intensity-based semiquantitative analysis the relative intensities of unique peptide signals were averaged to express individual protein abundance. Only proteins identified by two or more peptides were taken into account and the intensities of precursor ions with the best mascot score were used. Finally, the relative quantification index (RQI) representing the ratio of the resulting protein abundance between the AtFlot2-GFP plant sample and WT sample was calculated for each protein, and the proteins with RQI higher than three were considered to be enriched.
Split-Ubiquitin System
The Flot2 coding sequence was amplified from cDNA prepared from Col-0 using specific primers 3 and 4 (see Supplementary Table S1) and introduced in between the SalI and NotI restriction sites of the pENTR3c Dual Selection vector (Thermo Fisher) in a manner that allowed C-terminal protein fusion by restriction/ligation. LR recombination with the pMetYC-DEST vector encoding the C-terminal split-ubiquitin moiety as well as the LEU2 gene was then performed using GatewayTM LR ClonaseTM II Enzyme mix (Thermo Fisher) to obtain the final vector for yeast transformation.
Putative interactor coding sequences were amplified from cDNA prepared from Col-0 using specific primers 5–26 (see Supplementary Table S1) and introduced in between the KpnI and NotI (AtPIP2-6 and AtSYP71) or SalI and NotI (the rest of the sequences) restriction sites of the pENTR3c Dual Selection vector (Thermog Fisher) in a manner that allowed N-terminal protein fusion by restriction/ligation. LR recombination with a pNX35-DEST vector encoding the N-terminal split-ubiquitin moiety as well as the TRP1 gene was then performed using GatewayTM LR ClonaseTM II Enzyme mix (Thermo Fisher) to obtain the final vector for yeast transformation.
The THY.AP4 yeast strain was cotransformed (Hachez et al., 2014) with Flot2-pMetYC and X-pNX35 (X = investigated putative interactor of Flot2) vectors by the lithium acetate/single-stranded carrier DNA/PEG method (Grefen et al., 2009; Grefen, 2014) and plated on YNB + CSM (both MP Biomedicals) medium lacking Leu and Trp supplemented with 2% glucose and 50 μM Met. After a 2-day recovery at 30°C, freshly grown colonies were resuspended in milliQ water and diluted to obtain suspensions of optical densities (OD600) equal to 1.0, 0.1, and 0.01. Drops of 10 μl were placed on plates with YNB + CSM selective medium lacking Leu, Trp, Ade, and His, supplemented with 2% glucose and 50, 250, and 500 μM Met. Yeast growth was visually assessed after incubation for 2 days at 30°C.
The non-recombined pNX35 vector encoding for NubG, which was unable to reassemble with Cub co-transformed with Flot2-pMetYC, was used as the negative control, whereas the pNubWT-Xgate vector encoding for the wild-type Nub moiety spontaneously reassembling with Cub cotransformed with Flot2-pMetYC was used as the positive control in the SUS growth assay.
The pMetYC-DEST, pNX35-DEST, and pNubWT-Xgate vectors as well as the THY.AP4 yeast strain were kindly provided by Christopher Grefen, University of Tubingen, Germany. AtPIP2-7-pNX32 was kindly provided by François Chaumont and Timothée Laloux, Université catholique de Louvain, Belgium.
Results
Plasma Membrane Localization of Flot2-GFP
Since the only experimental evidence of the subcellular localization of Flot2 at the plasma membrane was found when YFP-fused A. thaliana Flot2 was transiently expressed in Nicotiana benthamiana leaf epidermal cells (Jarsch et al., 2014), we investigated the localization of Flot2-GFP directly in the epidermal cells of A. thaliana roots and cotyledons (Figure 1A). We observed that Flot2-GFP is predominantly localized at the plasma membrane in both of these diverse A. thaliana tissues. Flot2-GFP localization at plasma membrane was confirmed by subjecting root cells to the plasmolysis induced by mannitol; no Flot2-GFP signal was detected at the cell wall (Figure 1B). Therefore, not only the microsomal fraction, but also the enriched plasma membrane fraction was prepared to perform the IP-MS experiment. The lines overexpressing Flot2-GFP did not exhibit any apparent growth differences from Col-0 plants.
FIGURE 1
Enrichment of Flot2-GFP in Plasma Membrane Fractions
The microsomal fraction was isolated according to Qi and Katagiri (2009). A DSP cross-linker was used to fix the interacting proteins in the microsomal fraction before the dissolution of membranes with 0.5% (w/v) sodium deoxycholate. Due to the plasma membrane localization of Flot2, we also enriched the plasma membrane fraction with an extract from the cross-linked microsomal fraction, because the direct determination of its plasma membrane interactors could better contribute to the characterization of Flot2’s function. A flow chart of the procedure is depicted in Figure 2A.
FIGURE 2
During the isolation process, the presence of Flot2-GFP in each obtained fraction was detected by immunoblot analysis using the antibodies against GFP. Significant loss of the Flot2-GFP content caused by additional ultracentrifugation was observed between the native (line M) and cross-linked (line Mx) microsomal fraction (Figure 2B). We also analyzed the content of Flot2-GFP in fractions obtained after the dissolution of the cross-linked microsomal fraction by sodium deoxycholate; a higher amount of Flot2-GFP remained in the undissolved fraction (Figure 2C). Nevertheless, the total protein as well as Flot2-GFP content in the dissolved microsomal fraction was sufficient to perform the IP-MS analysis.
During the isolation of the plasma membrane fraction, a significantly lower yield of total proteins was obtained compared to the yield of proteins in the microsomal fraction. Finally, only approximately 2 μg of total proteins per 1 g of initial material were obtained in the enriched plasma membrane fraction. Nevertheless, the signal of Flot2-GFP in the enriched plasma membrane fraction (PMx line) was more intense than its signal in the cross-linked microsomal fractions (Mx line), which indicates that Flot2-GFP was successfully enriched in the plasma membrane fraction (Figure 2B).
Identification of Proteins Interacting With Flot2-GFP
Immunoprecipitation-MS of the microsomal as well as plasma membrane fraction was performed in nine repetitions. Only proteins which were detected at least three times in the immunoprecipitated AtFlot2-GFP membrane fractions and were not detected in the WT membrane fractions (control) were considered to be potential interactors of Flot2-GFP (Table 1). However, three additional proteins which were also detected in the control samples were included in the list of potential interactors, since they were significantly enriched in the AtFlot2-GFP sample according to MS intensity-based semiquantitative analysis. It can be seen in Table 1 that the IP-MS of the microsomal fraction provided a substantially lower number of potential interactors than the plasma membrane fraction. In total, 16 proteins were detected in this fraction, and only three of those were proposed to be Flot2 interaction partners. The majority of the detected proteins were actually only detected in one or two repetitions (Supplementary Table S2a). On the other hand, 61 proteins were enriched in the AtFlot-GFP plasma membrane fraction (Supplementary Table S2b). Of those, 19 proteins were proposed to be Flot2 interaction partners (Table 1).
Table 1
| F | Protein | Gene name | Locus | Counts | RQI | ||
|---|---|---|---|---|---|---|---|
| AtFlot2-GFP | WT | ||||||
| M | Flotillin 2 | FLOT2 | At5g25260 | 9 | – | – | |
| Ubiquitin-60S ribosomal protein L40-1 | RPL40A | At2g36170 | 5 | – | – | ||
| Aquaporin PIP2-1 | PIP2-1 | At3g53420 | 5 | – | – | ||
| Glyceraldehyde-3-phosphate dehydrogenase | GAPA1 | At3g26650 | 3 | – | – | ||
| PM | Flotillin 2 | FLOT2 | At5g25260 | 9 | – | – | |
| Photosystem I reaction center subunit II-2, chloroplastic | PSAD2 | At1g03130 | 5 | – | – | ||
| ATPase 1, plasma membrane-type | AHA1 | At2g18960 | 3 | – | – | ||
| Early-responsive to dehydration stress protein | ERD4 | At1g30360 | 3 | – | – | ||
| ABC transporter G family member 36 | ABCG36 | At1g59870 | 3 | – | – | ||
| Ubiquitin-60S ribosomal protein L40-1 | RPL40A | At2g36170 | 3 | – | – | ||
| Aquaporin PIP1-2 | PIP1-2 | At2g45960 | 3 | – | – | ||
| Syntaxin-71 | SYP71 | At3g09740 | 3 | – | – | ||
| Aquaporin PIP2-2 | PIP2-2 | At2g37170 | 3 | – | – | ||
| Harpin-induced protein-like | NHL3 | At5g06320 | 3 | – | – | ||
| Hypersensitive-induced response protein 2∗ | HIR2 | At3g01290 | 3 | – | – | ||
| Pyrophosphate-energized vacuolar membrane proton pump 1 | AVP1 | At1g15690 | 3 | – | – | ||
| Tubulin beta-5 chain | TUBB5 | At1g20010 | 3 | – | – | ||
| 5-methyltetrahydropteroyltri-glutamate-homocysteine methyltransferase 1 | MS1 | At5g17920 | 3 | – | – | ||
| Probable aquaporin PIP2-6 | PIP2-6 | At2g39010 | 3 | – | – | ||
| Probable inactive receptor kinase | – | At5g16590 | 3 | – | – | ||
| Aquaporin PIP2-1 | PIP2-1 | At3g53420 | 9 | 5 | 4.4 | ||
| Aquaporin PIP2-7 | PIP2-7 | At4g35100 | 8 | 3 | 3.5 | ||
| Carbonic anhydrase 2, chloroplastic | BCA2 | At5g14740 | 7 | 1 | 3.2 | ||
Potential interactors of Arabidopsis flotillin 2.
F, fraction; M, microsomal fraction; PM, plasma membrane fraction; Counts, number of IP repetitions (out of nine), in which the protein was detected; RQI, relative intensity index; ∗ At3g01290 is designated as HIR3 in UniProt database (www.uniprot.org).
To obtain greater insight into the proteins enriched by IP in both analyzed fractions (see Supplementary Table S2), a cluster analysis of GO annotation terms with respect to their localization and biological significance was performed using the DAVID Bioinformatics Resources annotation tool3 (Huang et al., 2009; Figure 3). Through the analysis of GO Cellular Component terms, it was found that the terms connected with the plasma membrane localization were only enriched when the plasma membrane fraction was used for the purification. This result shows the crucial importance of the appropriate fractioning of membrane proteins prior to their analysis. One of the most frequently occurring annotations of proteins purified from the plasma membrane fraction was localization in chloroplasts. When we mapped the proteins clustered within the chloroplast annotation, we found that half of them are simultaneously annotated to be localized in both, the plasma membrane and chloroplasts. Thus it is clear that the results of GO annotation cluster analysis can be influenced by the multiple annotations that exist for the proteins, and should therefore be carefully inspected.
FIGURE 3
According to the clustering of the GO Biological Process terms of proteins purified from the plasma membrane fraction, the potential interactors of Flot2 are predominantly involved in the plant response to various stress factors, such as the presence of cadmium ions, abscisic acid, bacteria, salt stress or water deprivation. Additionally, the response to water deprivation is the only process common to the proteins purified from both fractions.
Verification of Flot2 Interactions by Split-Ubiquitin Yeast System
After having determined the potential Flot2 interactors, we applied the yeast SUS to test whether some of the revealed proteins interact directly with Flot2. In the system used, Flot2 was C-terminally fused with the C-terminal moiety of ubiquitin (Cub) and hybrid transcription factor PLV (ProteinA-LexA-VP16), which enables yeast growth on the selection medium. The methionine-repressible vector allows expression tuning in order to avoid false positive results (Grefen et al., 2007). Selected potential interactors of Flot2 were N-terminally fused to the N-terminal moiety of mutated ubiquitin (NubG), which is prevented from spontaneously reassembling with Cub.
Twelve possible interactors from Table 1 were investigated by SUS, of which seven gave positive results (Figure 4). Among the five plasma membrane aquaporins, yeast growth was only observed for PIP1-2 and PIP2-6. Nevertheless, the yeast growth was relatively weak for both PIPs. This suggests a weak or very transient interaction between PIPs and Flot2. The strong yeast growth apparent for plasma membrane ATPase 1 (AHA1), early-responsive to dehydration (ERD) stress protein (ERD4), hypersensitive-induced response protein 2 (HIR2), harpin-induced protein-like (NHL3) and syntaxin-71 (SYP71) demonstrate physical interaction with Flot2. The expression of aquaporins PIP2-1, PIP2-2, and PIP2-7, pyrophosphate-energized vacuolar membrane proton pump 1 (AVP1) and probable inactive receptor kinase (At5g16590) in co-transformed yeasts was confirmed (see Supplementary Figure 1) to rule out the possibility that the lack of yeast growth observed in these cases was caused by a lack of Nub-fused prey proteins. The positive expression of all five putative interactors implies that none of these proteins would directly interact with Flot2.
FIGURE 4
Discussion
Flot2-GFP Cellular Localization
Although metazoan flotillins were found to be most frequently localized to plasma membrane microdomains or endosomes (Baumann et al., 2000; Dermine et al., 2001; Glebov et al., 2006; Neumann-Giesen et al., 2007; Langhorst et al., 2008), they were also rarely detected in the mitochondria or nucleus of human cells (Santamaria et al., 2005; Ogura et al., 2014). In plants, a similar localization was observed in several studies, where the A. thaliana Flot1, Picea meyeri Flot1, and Oryza sativa Flot1 were enriched in the plasma membrane DRM fraction (Triton X-100-insoluble plasma membrane fraction) prepared from A. thaliana calli, spruce pollen tubes, and rice cells (Borner et al., 2005; Liu P. et al., 2009; Ishikawa et al., 2015). While Flot1 localization to the plasma membrane was confirmed by the confocal microscopy of A. thaliana stably transformed with GFP-Flot1 (Li et al., 2012; Hao et al., 2014), the localization of Flot2 to the plasma membrane was only observed when YFP-fused Flot2 was transiently expressed in N. benthamiana leaf epidermal cells (Jarsch et al., 2014). Therefore, we investigated the localization of Flot2 directly in the epidermal cells of the roots and cotyledons of A. thaliana stably transformed with Flot2-GFP, and thus we confirmed its predominant localization to the plasma membrane (Figure 1).
Enrichment of Flot2-GFP Containing Complexes
Due to the many difficulties associated with the IP/AP-MS of membrane protein complexes, new approaches are being investigated (Qi and Katagiri, 2009; Smaczniak et al., 2012; Huang and Kim, 2013; Dorr et al., 2016). Because of the low abundance of membrane proteins, there is a need to use harsh conditions in order to ensure their efficient solubilization and release from the membrane. To maintain protein interactions under these conditions, chemical cross-linkers providing a covalent binding of proteins in complexes could be used. With plant cell suspension cultures or seedlings, in vivo cross-linking can be performed using membrane-permeable cross-linkers such as DSP (Jafferali et al., 2014; Kaake et al., 2014). Nevertheless, in vitro cross-linking has also been successfully applied, where full-grown plants were used as input material (Qi and Katagiri, 2009; Qi et al., 2011). The chemical cross-linking of protein complexes also enables more stringent conditions to be used during IP/AP to eliminate the non-specific background.
Whole plants or their specific tissues, organs or cell compartments need to be analyzed when the plant development pathways or pathways specific for tissue, cell type or cell compartment are being studied (Ephritikhine et al., 2004; Tan et al., 2008). Nevertheless, the presence of abundant soluble proteins during the purification step can greatly contribute to an increase in false positives and, additionally, could be also problematic in MS-based approaches, where soluble proteins are favored over hydrophobic and low-abundant membrane proteins (Gilmore and Washburn, 2010). Therefore, the isolation of the whole membrane fraction or individual membrane compartments should be performed prior to purification (Speers and Wu, 2007; Qi and Katagiri, 2009; Savas et al., 2011).
In our study, we used both the in vitro cross-linking of membrane proteins in close proximity to each other to maintain the interactions in complexes during sample preparation, and the enrichment of Flot2-GFP in microsomes and the plasma membrane fraction prior to IP-MS. Compared to the results obtained by the IP-MS of the microsomal fraction, we were able to capture a substantially higher number of interactors localized to the plasma membrane by IP-MS of the enriched plasma membrane (Figure 3). Despite the large amount of initial plant material entering the analysis, due to the low abundance of plasma membrane within plant membranes, together with the substantial loss of plant material during the isolation of the plasma membrane from whole plants, we believe that this direct approach is indispensable for a better description of plasma membrane complexes.
Determination of Flot2 Interactors
We used IP-MS to suggest potential interacting partners of Flot2. IP/AP-MS has become widely used nowadays, mainly thanks to the development of MS instrumentation that enables more efficient data acquisition. On the other hand, unfiltered IP/AP-MS data sets could give a large number of false positive interactions. To deal with this, a high number of repetitions and high number of controls should be analyzed (Pardo and Choudhary, 2012), different tags (Ho et al., 2002) as well as tag combinations (Van Leene et al., 2015) can be used, or some computational or informatics strategies can be applied for the evaluation of specific protein interactors (Collins and Choudhary, 2008; Choi H. et al., 2011; Nesvizhskii, 2012).
To identify specific interactors from the obtained IP-MS data set, some independent techniques such as Förster resonance energy transfer (FRET) or yeast two-hybrid assay can be used. In our study we suggested potential interactors by IP-MS and the specific interactors of Flot2 were then determined by SUS, a variant of yeast two-hybrid assay suitable for detecting a direct interaction between membrane-localized proteins.
Although SUS is far less used than the classical yeast two-hybrid test or bimolecular fluorescence complementation, it has been applied in more than 200 publications in major plant science journals to date (reviewed in Xing et al., 2016). Since SUS is a protein fragment complementation-based assay, there is a possibility of false positive (in comparison with e.g., FRET) as well as false negative results. To assess the possibility of false negative results (i.e., PIP2-1, PIP2-2, PIP2-7, AVP1, and At5g16590 in our study), it is necessary to keep in mind that the proper localization of both split ubiquitin moieties (to enable their reassembling at the cytoplasmatic side of the membrane) is a crucial prerequisite for the successful application of SUS. Therefore, the position of the N- or C-terminus of the investigated proteins (inside versus outside the cytoplasm) has to be considered when deciding, which protein terminus should be tagged with the split-ubiquitin moiety. In our study, all selected putative Flot2 interactors were fused at their N-terminus with NubG. In PIPs, both N- and C-terminal stretches are localized on the cytoplasmic sides of biomembranes (Murata et al., 2000; Luang and Hrmova, 2017), so tagging with NubG at each end is possible.
Nevertheless, proposing a suitable position for NubG fusion is tricky with AVP1 and At5g16590. AVP1 membrane topology prediction in tonoplasts suggests that both ends are localized inside vacuolar lumen (Pizzio et al., 2017). Hence, neither N- nor C-terminal fusion to NubG would be relevant for the interaction with cytoplasm-facing Cub. The structure of the At5g16590 protein is not published, and membrane-protein topology predictors do not provide unambiguous results (e.g., TMpred predicts the N-terminus in the cytoplasm while TMMOD predicts it on the extracellular side). On the other hand, At5g16590 belongs to a leucine-rich repeat kinase family, the majority of which have their N-terminal domains on the extracellular side of the plasma membrane (Diévart and Clark, 2003). However, several interactors were found for both NubG- and Cub-fused AVP1 and At5g16590 protein in Associomics. Thus, additional SUS assays with AVP1 and At5g16590 fused to NubG at their C-terminus will be necessary to further verify the interaction with Flot2 and potentially rule out the results found in this study as a false negative.
Flot2 Interactors Are Found in Specific Plasma Membrane Subfractions
In our study, Flot2-GFP was found to be enriched in the deoxycholate-insoluble part of the microsomal fraction and the enriched plasma membrane fraction (Figure 2). Correspondingly most of the putative interactors found in our screen were already identified in membrane fractions resistant to mild detergents. AHA1, SYP71, NHL3, ERD4, PIP1-2, PIP2-7, and HIR2 as well as other HIR homologs HIR1 and HIR4 were enriched in the plasma membrane DRM fraction obtained from Arabidopsis plants, calli or suspension cells (Shahollari et al., 2004; Borner et al., 2005; Keinath et al., 2010). Moreover, homologs of SYP71, AHA1, and several PIPs were found in the plasma membrane DRM fraction from tobacco leaves (Mongrand et al., 2004). Although Flot2 was not identified in any of those studies, its closest homolog Flot1 (Yu et al., 2017) was reported to be present in similarly prepared plasma membrane fractions (Borner et al., 2005; Ishikawa et al., 2015). Additionally, rice Flot was also enriched when plasma membrane DRM fractions were prepared from rice (Ishikawa et al., 2015). Additionally, AHA1 and HIR2 together with AHA2, HIR1, and HIR4 were identified as major proteins tightly associated with a plasma membrane resistant to NaCl and Na2CO3 washing (Marmagne et al., 2007). Such a co-occurrence in specific plasma membrane sub-compartments may suggest a functional linkage in many cellular processes.
Identified Interactors Suggest Putative Functions
Flot2 transcription is highly upregulated upon bacterial, fungal, viral and oomycetal infection (Mohr and Cahill, 2007; Danek et al., 2016). Its transcription together with that of SYP71, HIR2, NHL3 and Flot3, HIR1, HIR3, and HIR4 were increased under viral infection (Ascencio-Ibanez et al., 2008). Increased HIR2, Flot2 as well as Flot1 transcription in mutants with altered systemic acquired resistance also suggests their involvement in this type of defense mechanism (Mosher et al., 2006). Moreover, the content of direct Flot2 interactors SYP71, AHA1, NHL3, ERD4, HIR2, and the HIR1 and HIR4 content in the plasma membrane DRM fraction was increased after treatment with the bacteria-derived elicitor flg22 (Keinath et al., 2010). Several of these interactors have been reported to be involved in resistance against pathogens.
Flot2 has already been found to directly interact with SYP71, a member of a plant-specific subfamily of Qc SNARE proteins (Sanderfoot et al., 2001). SYP71 transcription is increased under viral infection (Ascencio-Ibanez et al., 2008) and it plays a role in viral protein within the cell (Wei et al., 2013). Wheat and rice SYP71 homologs confer resistance to stripe rust and blast, respectively, and their transcription is upregulated upon infection by the respective pathogens, as well as upon treatment with hydrogen peroxide (Bao et al., 2012; Liu et al., 2016), a hallmark of hypersensitive plant defense (Coll et al., 2011). Lotus japonicus SYP71 is important for proper nodulation in Mesorhizobium loti symbiosis (Hakoyama et al., 2012). Interestingly, M. truncatula Flot2 and Flot4 are involved in nodulation, probably due to an interaction with an activated nodulation factor receptor (Haney and Long, 2010; Haney et al., 2011).
HIR2, another direct interactor of Flot2, belongs to the subfamily of SPFH proteins and is thus related to flotillins (Di et al., 2010). Four Arabidopsis HIRs interact with one another and HIR2 and HIR1 directly interact with the immune receptor RPS2. The interaction participates in effector-triggered resistance against Pseudomonas syringae (Qi et al., 2011). HIR homologs in pepper, rice and barley mediate the hypersensitive response to pathogens via an interaction with leucine-rich repeat proteins (Jung and Hwang, 2007; Zhou et al., 2009, 2010; Choi H.W. et al., 2011; Cheng et al., 2017).
NHL3 (NDR1/HIN1-LIKE 3) is another directly interacting protein involved in the plant–pathogen interaction. The transcription of NHL3 is induced by salicylic acid treatment, bacterial infection (Varet et al., 2002; Ditt et al., 2006), hydrogen peroxide treatment (Davletova et al., 2005) and by spermine, a polyamine signaling molecule inducing the expression of pathogenesis-related genes (Zheng et al., 2004). The overexpression of NHL3 leads to increased resistance to P. syringae. NHL3 was shown to be tightly associated with the plasma membrane (Varet et al., 2003), where it physically interacts with the oxidation-related zinc finger one protein that is also involved in salicylic acid-mediated defense reactions to bacterial attack (Singh et al., 2018).
The plasma membrane-localized H+ATPase AHA1 is a major proton pump contributing to stomata opening (Yamauchi et al., 2016), which makes it also closely connected with pathogen resistance reactions. AHA1 activity is altered by methyl jasmonate treatment (Yan et al., 2015) and binding to the bacterial effector AvrB (Zhou et al., 2015) or RIN4, a target of bacterial effectors (Liu J. et al., 2009). AHA1 together with HIR2 and HIR4 co-immunoprecipitated with HIR1 (Lv et al., 2017) and with Bax Inhibitor 1, an ER-localized suppressor of cell death after fungal infection (Weis et al., 2013). Interestingly, the overexpression of Bax Inhibitor 1 in rice cells resulted in the depletion of rice Flot and HIR homologs from DRM (Ishikawa et al., 2015). These findings suggest the involvment of HIRs, Flots and AHA1 in a shared pathway controling cell death and/or the reaction to pathogen attack.
We observed a direct interaction with two (PIP1-2 and PIP2-6) of five PIPs co-immunoprecipitating with Flot2. PIP1-2, PIP2-1, PIP2-2, and PIP2-7 transcription is significantly decreased under drought stress, whereas PIP2-6 transcription does not change (Alexandersson et al., 2005). PIP1-2 alone contributes, but due to functional redundancy with other PIPs, it is not essential for plant growth or water transport (Postaire et al., 2010); however, it is important for CO2 permeability and thus for the net photosynthesis rate (Heckwolf et al., 2011; Uehlein et al., 2012).
Besides this, the proper cellular trafficking of PIP2-7 and PIP2-2 between endomembranes and the plasma membrane is dependent on direct interaction with SYP121 and SYP61 (Hachez et al., 2014). A similar requirement was reported for maize PIP2-5 (Besserer et al., 2012). SYP121 also directly interacts with PIP2-2 (Besserer et al., 2012) and co-immunoprecipitates with SYP71 as well as some other SYPs, and, moreover, with HIR2 (Fujiwara et al., 2014). In addition, functional aquaporins are formed as tetramers, where the hetero-tetramerization of several single PIP isoforms has been reported (Jozefkowicz et al., 2017). The hetero-oligomerization of PIP1 and PIP2 group aquaporins is necessary for the trafficking of maize PIP1s to the plasma membrane (Zelazny et al., 2007).
PIP2-1 and PIP1-2 interact with several hundred proteins. Physical interaction was confirmed for PIP2-1 and NHL3 (Bellati et al., 2016), and three HIRs were detected to co-immunoprecipitate with at least one of PIP2-1 and PIP1-2. Therefore, a putative indirect linkage between PIP2-1, PIP2-2, PIP2-7, and Flot2 may be realized via PIP2-6 and PIP1-2, via SYP71 and SYP121 (with or without the involvement of HIR2), or via NHL3 or HIR2. Moreover, PIP2-1 transcription in roots is decreased upon exposure to NaCl (Boursiac et al., 2005) and PIP2-1 was observed to be endocytosed from the plasma membrane upon NaCl treatment via Flot1-mediated endocytosis (Li et al., 2011). A similar involvement of Flot1 in clathrin-independent endocytosis was observed for the ammonium transporter AMT1-3 (Wang et al., 2015). Interestingly AMT1-3 was also found to interact with several Flot2 interactors determined in our study (Bellati et al., 2016). The function of Flot1 endocytosis remains unclear, but based on the very close similarity of both isoforms, it is possible that a Flot2-based complex can be implicated in similar processes.
AHA1 also contributes to water transport by its direct interaction with phytosulfokine receptors PSKR1 and PSKR2 and cyclic nucleotide-gated channel 17, a receptor for cyclic guanosine monophosphate. The application of both signal ligands leads to increased water influx, which is important for the volume growth of plant cells (Ladwig et al., 2015). Similarly, AHA1 is phosphorylated with the activated receptor of the peptide plant regulator PSY1R, which leads to root and hypocotyl elongation (Fuglsang et al., 2014). Proper AHA1 trafficking to the plasma membrane is important for plant growth, as plants with AHA1 accumulated in their endomembranes are smaller in size (Hashimoto-Sugimoto et al., 2013).
The participation of Flot2 in water management is also suggested by the direct interaction between Flot2 and ERD4, a member of the ERD protein family (Kiyosue et al., 1994). Enhanced tolerance to drought and salt stress was observed in Arabidopsis overexpressing maize ERD4 (Liu Y. et al., 2009).
Although the identification of Flot2 specific interactors can suggest a potential role of Flot2 in A. thaliana, further studies are required. Phenotypic analysis of Flot2 loss-of function mutants could be a valuable approach. We initially tested several abiotic and biotic treatments using a flot2 T-DNA insertion line but did not observe any major effect different from wild type plants. Since there are three isoforms of flotillins encoded in A. thaliana, functional redundancy of these single isoforms may be an explanation for this lack of phenotype. Generation of multiple mutants may thus be necessary.
Flot2 Interactome Forms a Complex Interlinked Network
As has already been pointed out, proteins found to interact with Flot2 in this study may also in many cases interact with each other, and thus it is possible that a given protein may be involved in several cellular functions. The situation gets even more complicated when direct interactors of each protein retrieved from the Associomic database are added to the list (Figure 5). Interestingly, it could be seen in Associomics that many of the Flot2 interactors found in this study also interact with other Flot2 interactors listed in Associomics. The most common interactors are NHL3, cornichon and IQD6. Since these proteins have several hundred interactors in Associomics, they could serve as real docking hubs for many proteins in the plasma membrane, and could thus be the crossroads or signposts of many pathways. On the other hand, it is possible that these proteins might be just too prone to giving false positive results. Intriguingly, the direct interactions of Flot2 that we confirmed in our study are not proposed for Flot2 in Associomics. Similarly, the direct interaction of NHL3 with PIP2-1 published in Bellati et al. (2016) is not found in Associomics. This comparison in fact demonstrates the general importance of the IP approach to membrane protein interactome determination.
FIGURE 5
Statements
Author contributions
JB, DK, and MD generated plant material. PJ, MH, RH, and OV contributed to the proteomic analysis and evaluation of results. MD, EZ, and KK prepared and performed SUS assays. PJ, MD, MJ, OV, and JM wrote the manuscript. PJ and MD contributed equally to this article.
Funding
This work was supported by the Czech Science Foundation grant no. 14-09685S and by the Program Barrande grant no. 7AMB17FR005 provided by the Czech Ministry of Education, Youth and Sports and by French Ministry for Europe and Foreign Affairs (MEAE), and Ministry of Higher Education, Research and Innovation (MESRI).
Acknowledgments
The authors would like to thank Christopher Grefen, University of Tuebingen, Germany and François Chaumont and Timothée Laloux, Université catholique de Louvain, Belgium for sharing vectors and the yeast strain used in SUS assay as well as Kateřina Vltavská, Institute of Experimental Botany, Czech Republic, for her excellent technical assistance.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2018.00991/full#supplementary-material
References
1
AlexanderssonE.FraysseL.Sjovall-LarsenS.GustavssonS.FellertM.KarlssonM.et al (2005). Whole gene family expression and drought stress regulation of aquaporins.Plant Mol. Biol.59469–484. 10.1007/s11103-005-0352-1
2
AmaddiiM.MeisterM.BanningA.TomasovicA.MoozJ.RajalingamK.et al (2012). Flotillin-1/reggie-2 protein plays dual role in activation of receptor-tyrosine kinase/mitogen-activated protein kinase signaling.J. Biol. Chem.2877265–7278. 10.1074/jbc.M111.287599
3
Ascencio-IbanezJ. T.SozzaniR.LeeT. J.ChuT. M.WolfingerR. D.CellaR.et al (2008). Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.Plant Physiol.148436–454. 10.1104/pp.108.121038
4
BaoY. M.SunS. J.LiM.LiL.CaoW. L.LuoJ.et al (2012). Overexpression of the Qc-SNARE gene OsSYP71 enhances tolerance to oxidative stress and resistance to rice blast in rice (Oryza sativa L.).Gene504238–244. 10.1016/j.gene.2012.05.011
5
BaumannC. A.RibonV.KanzakiM.ThurmondD. C.MoraS.ShigematsuS.et al (2000). CAP defines a second signalling pathway required for insulin-stimulated glucose transport.Nature407202–207. 10.1038/35025089
6
BellatiJ.ChampeyrouxC.HemS.RofidalV.KroukG.MaurelC.et al (2016). Novel aquaporin regulatory mechanisms revealed by interactomics.Mol. Cell. Proteomics153473–3487. 10.1074/mcp.M116.060087
7
BessererA.BurnotteE.BienertG. P.ChevalierA. S.ErrachidA.GrefenC.et al (2012). Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121.Plant Cell243463–3481. 10.1105/tpc.112.101758
8
BickelP. E.SchererP. E.SchnitzerJ. E.OhP.LisantiM. P.LodishH. F. (1997). Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins.J. Biol. Chem.27213793–13802. 10.1074/jbc.272.21.13793
9
BornerG. H. H.SherrierD. J.WeimarT.MichaelsonL. V.HawkinsN. D.MacAskillA.et al (2005). Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts.Plant Physiol.137104–116. 10.1104/pp.104.053041
10
BoursiacY.ChenS.LuuD. T.SorieulM.van den DriesN.MaurelC. (2005). Early effects of salinity on water transport in Arabidopsis roots. molecular and cellular features of aquaporin expression.Plant Physiol.139790–805. 10.1104/pp.105.065029
11
BrowmanD. T.HoeggM. B.RobbinsS. M. (2007). The SPFH domain-containing proteins: more than lipid raft markers.Trends Cell Biol.17394–402. 10.1016/j.tcb.2007.06.005
12
CacasJ. L.BureC.GrosjeanK.Gerbeau-PissotP.LherminierJ.RomboutsY.et al (2016). Revisiting plant plasma membrane lipids in tobacco: a focus on sphingolipids.Plant Physiol.170367–384. 10.1104/pp.15.00564
13
ChengW.XiaoZ. L.CaiH. Y.WangC. Q.HuY.XiaoY. P.et al (2017). A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection.Mol. Plant Pathol.181089–1100. 10.1111/mpp.12462
14
ChoiH.LarsenB.LinZ. Y.BreitkreutzA.MellacheruvuD.FerminD.et al (2011). SAINT: probabilistic scoring of affinity purification-mass spectrometry data.Nat. Methods870–73. 10.1038/Nmeth.1541
15
ChoiH. W.KimY. J.HwangB. K. (2011). The Hypersensitive induced reaction and leucine-rich repeat proteins regulate plant cell death associated with disease and plant immunity.Mol. Plant Microbe Interact.2468–78. 10.1094/MPMI-02-10-0030
16
CollN. S.EppleP.DanglJ. L. (2011). Programmed cell death in the plant immune system.Cell Death Differ.181247–1256. 10.1038/cdd.2011.37
17
CollinsM. O.ChoudharyJ. S. (2008). Mapping multiprotein complexes by affinity purification and mass spectrometry.Curr. Opin. Biotechnol.19324–330. 10.1016/j.copbio.2008.06.002
18
DanekM.ValentovaO.MartinecJ. (2016). Flotillins, erlins, and hirs: from animal base camp to plant new horizons.Crit. Rev. Plant Sci.35191–214. 10.1080/07352689.2016.1249690
19
DavletovaS.SchlauchK.CoutuJ.MittlerR. (2005). The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis.Plant Physiol.139847–856. 10.1104/pp.105.068254
20
DedeckerM.Van LeeneJ.De JaegerG. (2015). Unravelling plant molecular machineries through affinity purification coupled to mass spectrometry.Curr. Opin. Plant Biol.241–9. 10.1016/j.pbi.2015.01.001
21
DermineJ. F.DuclosS.GarinJ.St-LouisF.ReaS.PartonR. G.et al (2001). Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes.J. Biol. Chem.27618507–18512. 10.1074/jbc.M101113200
22
DiC.XuW. Y.SuZ.YuanJ. S. (2010). Comparative genome analysis of PHB gene family reveals deep evolutionary origins and diverse gene function.BMC Bioinformatics11(Suppl. 6):S22.10.1186/1471-2105-11-s6-s22
23
DiévartA.ClarkS. E. (2003). Using mutant alleles to determine the structure and function of leucine-rich repeat receptor-like kinases.Curr. Opin. Plant Biol.6507–516. 10.1016/S1369-5266(03)00089-X
24
DittR. F.KerrK. F.de FigueiredoP.DelrowJ.ComaiL.NesterE. W. (2006). The Arabidopsis thaliana transcriptome in response to Agrobacterium tumefaciens.Mol. Plant Microbe Interact.19665–681. 10.1094/MPMI-19-0665
25
DorrJ. M.ScheidelaarS.KoorengevelM. C.DominguezJ. J.SchaferM.van WalreeC. A.et al (2016). The styrene-maleic acid copolymer: a versatile tool in membrane research.Eur. Biophys. J.453–21. 10.1007/s00249-015-1093-y
26
DunhamW. H.MullinM.GingrasA. C. (2012). Affinity-purification coupled to mass spectrometry: basic principles and strategies.Proteomics121576–1590. 10.1002/pmic.201100523
27
EphritikhineG.FerroM.RollandN. (2004). Plant membrane proteomics.Plant Physiol. Biochem.42943–962. 10.1016/j.plaphy.2004.11.004
28
FuglsangA. T.KristensenA.CuinT. A.SchulzeW. X.PerssonJ.ThuesenK. H.et al (2014). Receptor kinase-mediated control of primary active proton pumping at the plasma membrane.Plant J.80951–964. 10.1111/tpj.12680
29
FujiwaraM.UemuraT.EbineK.NishimoriY.UedaT.NakanoA.et al (2014). Interactomics of Qa-SNARE in Arabidopsis thaliana.Plant Cell Physiol.55781–789. 10.1093/pcp/pcu038
30
GehlB.LeeC. P.BotaP.BlattM. R.SweetloveL. J. (2014). An Arabidopsis stomatin-like protein affects mitochondrial respiratory supercomplex organization.Plant Physiol.1641389–1400. 10.1104/pp.113.230383
31
GilmoreJ. M.WashburnM. P. (2010). Advances in shotgun proteomics and the analysis of membrane proteomes.J. Proteomics732078–2091. 10.1016/j.jprot.2010.08.005
32
GlebovO. O.BrightN. A.NicholsB. J. (2006). Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells.Nat. Cell Biol.846–54. 10.1038/ncb1342
33
GreenJ. B.YoungJ. P. W. (2008). Slipins: ancient origin, duplication and diversification of the stomatin protein family.BMC Evol. Biol.8:44. 10.1186/1471-2148-8-44
34
GrefenC. (2014). “The split-ubiquitin system for the analysis of three-component interactions,” inArabidopsis Protocols, edsSanchez-SerranoJ. J.SalinasJ. (Totowa, NJ: Humana Press) 659–678.
35
GrefenC.LalondeS.ObrdlikP. (2007). Split-ubiquitin system for identifying protein-protein interactions in membrane and full-length proteins.Curr. Protoc. Neurosci.415.27.1–5.27.41. 10.1002/0471142301.ns0527s41
36
GrefenC.ObrdlikP.HarterK. (2009). “The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS),” inPlant Signal Transduction: Methods and Protocols, ed.PfannschmidtT. (Totowa, NJ: Humana Press), 217–233.
37
HachezC.LalouxT.ReinhardtH.CavezD.DegandH.GrefenC.et al (2014). Arabidopsis SNAREs SYP61 and SYP121 coordinate the trafficking of plasma membrane aquaporin PIP2;7 to modulate the cell membrane water permeability.Plant Cell263132–3147. 10.1105/tpc.114.127159
38
HakoyamaT.OiR.HazumaK.SugaE.AdachiY.KobayashiM.et al (2012). The SNARE protein SYP71 expressed in vascular tissues is involved in symbiotic nitrogen fixation in Lotus japonicus nodules.Plant Physiol.160897–905. 10.1104/pp.112.200782
39
HaneyC. H.LongS. R. (2010). Plant flotillins are required for infection by nitrogen-fixing bacteria.Proc. Natl. Acad. Sci. U.S.A.107478–483. 10.1073/pnas.0910081107
40
HaneyC. H.RielyB. K.TricoliD. M.CookD. R.EhrhardtD. W.LongS. R. (2011). Symbiotic rhizobia bacteria trigger a change in localization and dynamics of the Medicago truncatula receptor kinase LYK3.Plant Cell232774–2787. 10.1105/tpc.111.086389
41
HaoH. Q.FanL. S.ChenT.LiR. L.LiX. J.HeQ. H.et al (2014). Clathrin and membrane microdomains cooperatively regulate rbohd dynamics and activity in Arabidopsis.Plant Cell261729–1745. 10.1105/tpc.113.122358
42
Hashimoto-SugimotoM.HigakiT.YaenoT.NagamiA.IrieM.FujimiM.et al (2013). A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses.Nat. Commun.4:2215. 10.1038/ncomms3215
43
HeckwolfM.PaterD.HansonD. T.KaldenhoffR. (2011). The Arabidopsis thaliana aquaporin AtPIP1;2 is a physiologically relevant CO2 transport facilitator.Plant J.67795–804. 10.1111/j.1365-313X.2011.04634.x
44
HoY.GruhlerA.HeilbutA.BaderG. D.MooreL.AdamsS. L.et al (2002). Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.Nature415180–183. 10.1038/415180a
45
HuangB. X.KimH. Y. (2013). Effective identification of Akt interacting proteins by two-step chemical crosslinking, co-immunoprecipitation and mass spectrometry.PLoS One8:e61430. 10.1371/journal.pone.0061430
46
HuangD. W.ShermanB. T.LempickiR. A. (2009). Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.Nat. Protoc.444–57. 10.1038/nprot.2008.211
47
IshikawaT.AkiT.YanagisawaS.UchimiyaH.Kawai-YamadaM. (2015). Overexpression of BAX INHIBITOR-1 links plasma membrane microdomain proteins to stress.Plant Physiol.1691333–1343. 10.1104/pp.15.00445
48
JafferaliM. H.VijayaraghavanB.FigueroaR. A.CrafoordE.GudiseS.LarssonV. J.et al (2014). MCLIP, an effective method to detect interactions of transmembrane proteins of the nuclear envelope in live cells.Biochim. Biophys. Acta18382399–2403. 10.1016/j.bbamem.2014.06.008
49
JarschI. K.KonradS. S. A.StratilT. F.UrbanusS. L.SzymanskiW.BraunP.et al (2014). Plasma membranes are subcompartmentalized into a plethora of coexisting and diverse microdomains in Arabidopsis and Nicotiana benthamiana.Plant Cell261698–1711. 10.1105/tpc.114.124446
50
JonesA. M.XuanY.XuM.WangR.-S.HoC.-H.LalondeS.et al (2014). Border control-a membrane-linked interactome of Arabidopsis.Science344711–716. 10.1126/science.1251358
51
JozefkowiczC.BernyM.C.ChaumontF.AllevaK. (2017). “Heteromerization of plant aquaporins,” inPlant Aquaporins, edsChaumontF.TyermanS. D. (Berlin: Springer International Publishing), 29–46. 10.1007/978-3-319-49395-4_2
52
JungH. W.HwangB. K. (2007). The leucine-rich repeat (LRR) protein, CaLRR1, interacts with the hypersensitive induced reaction (HIR) protein, CaHIR1, and suppresses cell death induced by the CaHIR1 protein.Mol. Plant Pathol.8503–514. 10.1111/j.1364-3703.2007.00410.x
53
KaakeR. M.WangX. R.BurkeA.YuC.KandurW.YangY. Y.et al (2014). A new in vivo cross-linking mass spectrometry platform to define protein-protein interactions in living cells.Mol. Cell. Proteomics133533–3543. 10.1074/mcp.M114.042630
54
KeinathN. F.KierszniowskaS.LorekJ.BourdaisG.KesslerS. A.Shimosato-AsanoH.et al (2010). PAMP (Pathogen-Associated Molecular Pattern)-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity.J. Biol. Chem.28539140–39149. 10.1074/jbc.M110.160531
55
KiyosueT.Yamaguchi-ShinozakiK.ShinozakiK. (1994). Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs as HSP cognate genes.Plant Mol. Biol.25791–798. 10.1007/bf00028874
56
LadwigF.DahlkeR. I.StuhrwohldtN.HartmannJ.HarterK.SauterM. (2015). Phytosulfokine regulates growth in Arabidopsis through a response module at the plasma membrane that includes cyclic nucleotide-gated CHANNEL17, H+-ATPase, and BAK1.Plant Cell271718–1729. 10.1105/tpc.15.00306
57
LanghorstM. F.ReuterA.JaegerF. A.WippichF. M.LuxenhoferG.PlattnerH.et al (2008). Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2.Eur. J. Cell Biol.87211–226. 10.1016/j.ejcb.2007.12.001
58
LiN.HuangX.ZhaoZ. L.ChenG. Y.ZhangW. P.CaoX. T. (2000). Identification and characterization of a novel gene KE04 differentially expressed by activated human dendritic cells.Biochem. Biophys. Res. Commun.279487–493. 10.1006/bbrc.2000.3935
59
LiR. L.LiuP.WanY. L.ChenT.WangQ. L.MettbachU.et al (2012). A membrane microdomain-associated protein, Arabidopsis Flot1, is involved in a clathrin-independent endocytic pathway and is required for seedling development.Plant Cell242105–2122. 10.1105/tpc.112.095695
60
LiX. J.WangX. H.YangY.LiR. L.HeQ. H.FangX. H.et al (2011). Single-molecule analysis of pip2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation.Plant Cell233780–3797. 10.1105/tpc.111.091454
61
LiuJ.DeYoungS. M.ZhangM.DoldL. H.SaltielA. R. (2005). The stomatin/prohibitin/flotillin/HflK/C domain of flotillin-1 contains distinct sequences that direct plasma membrane localization and protein interactions in 3T3-L1 adipocytes.J. Biol. Chem.28016125–16134. 10.1074/jbc.M500940200
62
LiuJ.ElmoreJ. M.FuglsangA. T.PalmgrenM. G.StaskawiczB. J.CoakerG. (2009). RIN4 functions with plasma membrane H+-atpases to regulate stomatal apertures during pathogen attack.PLoS Biol.7:e1000139. 10.1371/journal.pbio.1000139
63
LiuM. J.PengY.LiH. Y.DengL.WangX. J.KangZ. S. (2016). TaSYP71, a Qc-SNARE, contributes to wheat resistance against Puccinia striiformis f. sp tritici.Front. Plant Sci.7:544. 10.3389/fpls.2016.00544
64
LiuP.LiR. L.ZhangL.WangQ. L.NiehausK.BaluskaF.et al (2009). Lipid microdomain polarization is required for NADPH oxidase-dependent ROS signaling in Picea meyeri pollen tube tip growth.Plant J.60303–313. 10.1111/j.1365-313X.2009.03955.x
65
LiuY.LiH.ShiY.SongY.WangT.LiY. (2009). A maize early responsive to dehydration gene, ZmERD4, provides enhanced drought and salt tolerance in Arabidopsis.Plant Mol. Biol. Rep.27542–548. 10.1007/s11105-009-0119-y
66
LuangS.HrmovaM. (2017). “Structural basis of the permeation function of plant aquaporins,” inPlant Aquaporins, edsChaumontF.TyermanS. D. (Berlin: Springer International Publishing), 1–28. 10.1007/978-3-319-49395-4_1
67
LvX. Q.JingY. P.XiaoJ. W.ZhangY. D.ZhuY. F.JulianR.et al (2017). Membrane microdomains and the cytoskeleton constrain AtHIR1 dynamics and facilitate the formation of an AtHIR1-associated immune complex.Plant J.903–16. 10.1111/tpj.13480
68
MarmagneA.FerroM.MeinnelT.BruleyC.KuhnL.GarinJ.et al (2007). A high content in lipid-modified peripheral proteins and integral receptor kinases features in the Arabidopsis plasma membrane proteome.Mol. Cell. Proteomics61980–1996. 10.1074/mcp.M700099-MCP200
69
MohrP. G.CahillD. M. (2007). Suppression by ABA of salicylic acid and lignin accumulation and the expression of multiple genes, in Arabidopsis infected with Pseudomonas syringae pv.tomato. Funct. Integr. Genomics7181–191. 10.1007/s10142-006-0041-4
70
MongrandS.MorelJ.LarocheJ.ClaverolS.CardeJ. P.HartmannM. A.et al (2004). Lipid rafts in higher plant cells – purification and characterization of triton X-100-insoluble microdomains from tobacco plasma membrane.J. Biol. Chem.27936277–36286. 10.1074/jbc.M403440200
71
MorrowI. C.ReaS.MartinS.PriorI. A.ProhaskaR.HancockJ. F.et al (2002). Flotillin-1/Reggie-2 traffics to surface raft domains via a novel Golgi-independent pathway – identification of a novel membrane targeting domain and a role for palmitoylation.J. Biol. Chem.27748834–48841. 10.1074/jbc.M209082200
72
MosherR. A.DurrantW. E.WangD.SongJ. Q.DongX. N. (2006). A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.Plant Cell181750–1765. 10.1105/tpc.105.039677
73
MurataK.MitsuokaK.HiraiT.WalzT.AgreP.HeymannJ. B.et al (2000). Structural determinants of water permeation through aquaporin-1.Nature407599–605. 10.1038/35036519
74
NesvizhskiiA. I. (2012). Computational and informatics strategies for identification of specific protein interaction partners in affinity purification mass spectrometry experiments.Proteomics121639–1655. 10.1002/pmic.201100537
75
Neumann-GiesenC.FalkenbachB.BeichtP.ClaasenS.LuersG.StuermerC. A. O.et al (2004). Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression.Biochem. J.378509–518. 10.1042/bj20031100
76
Neumann-GiesenC.FernowI.AmaddiiM.TikkanenR. (2007). Role of EGF-induced tyrosine phosphorylation of reggie-1/flotillin-2 in cell spreading and signaling to the actin cytoskeleton.J. Cell Sci.120395–406. 10.1242/jcs.03336
77
OguraM.YamakiJ.HommaM. K.HommaY. (2014). Phosphorylation of flotillin-1 by mitochondrial c-Src is required to prevent the production of reactive oxygen species.FEBS Lett.5882837–2843. 10.1016/j.febslet.2014.06.044
78
PardoM.ChoudharyF. S. (2012). Assignment of protein interactions from affinity purification/mass spectrometry data.J. Proteome Res.111462–1474. 10.1021/pr2011632
79
PeremyslovV. V.MorgunE. A.KurthE. G.MakarovaK. S.KooninE. V.DoljaV. V. (2013). Identification of myosin XI receptors in Arabidopsis defines a distinct class of transport vesicles.Plant Cell253022–3038. 10.1105/tpc.113.113704
80
PizzioG. A.HirschiK. D.GaxiolaR. A. (2017). Conjecture regarding posttranslational modifications to the Arabidopsis type I Proton-pumping pyrophosphatase (AVP1). Front. Plant Sci.8:1572. 10.3389/fpls.2017.01572
81
PleskotR.PotockyM.PejcharP.LinekJ.BezvodaR.MartinecJ.et al (2010). Mutual regulation of plant phospholipase D and the actin cytoskeleton.Plant J.62494–507. 10.1111/j.1365-313X.2010.04168.x
82
PopovN.SchmittM.SchulzeckS.MatthiesH. (1975). Reliable micromethod for determining protein-content in tissue material.Acta Biol. Med. Ger.341441–1446.
83
PostaireO.Tournaire-RouxC.GrondinA.BoursiacY.MorillonR.SchaffnerA. R.et al (2010). A PIP1 aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of Arabidopsis.Plant Physiol.1521418–1430. 10.1104/pp.109.145326
84
QiY. P.KatagiriF. (2009). Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components.Plant J.57932–944. 10.1111/j.1365-313X.2008.03736.x
85
QiY. P.TsudaK.NguyenL. V.WangX.LinJ. S.MurphyA. S.et al (2011). Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2.J. Biol. Chem.28631297–31307. 10.1074/jbc.M110.211615
86
RoitbakT.SurviladzeZ.TikkanenR.Wandinger-NessA. (2005). A polycystin multiprotein complex constitutes a cholesterol-containing signalling microdomain in human kidney epithelia.Biochem. J.39229–38. 10.1042/bj20050645
87
SanderfootA. A.KovalevaV.BasshamD. C.RaikhelN. V. (2001). Interactions between syntaxins identify at least five SNARE complexes within the golgi/prevacuolar system of the Arabidopsis cell.Mol. Biol. Cell123733–3743. 10.1091/mbc.12.12.3733
88
SantamariaA.CastellanosE.GomezV.BeneditP.Renau-PiquerasJ.MoroteJ.et al (2005). PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts.Mol. Cell Biol.251900–1911. 10.1128/Mcb.25.5.1900-1911.2005
89
SavasJ. N.SteinB. D.WuC. C.YatesJ. R. (2011). Mass spectrometry accelerates membrane protein analysis.Trends Biochem. Sci.36388–396. 10.1016/j.tibs.2011.04.005
90
ShahollariB.Peskan-BerghöferT.OelmüllerR. (2004). Receptor kinases with leucine-rich repeats are enriched in Triton X-100 insoluble plasma membrane microdomains from plants.Physiol. Plant.122397–403. 10.1111/j.1399-3054.2004.00414.x
91
SchindlerJ.NothwangH. G. (2006). Aqueous polymer two-phase systems: effective tools for plasma membrane proteomics.Proteomics65409–5417. 10.1002/pmic.200600243
92
SchroederW. T.StewartgaletkaS.MandavilliS.ParryD. A. D.GoldsmithL.DuvicM. (1994). Cloning and characterization of a novel epidermal-cell surface-antigen (Esa).J. Biol. Chem.26919983–19991.
93
SchulteT.LottspeichF.StuermerC. A. O. (1995). Characterization of reggie-1 and isolation of reggie-2, cell surface proteins of the goldfish CNS.Soc. Neurosci. Abstr.21:795.
94
SimonsK.IkonenE. (1997). Functional rafts in cell membranes.Nature387569–572. 10.1038/42408
95
SimonsK.ToomreD. (2000). Lipid rafts and signal transduction.Nat. Rev. Mol. Cell Biol.131–39. 10.1038/35036052
96
SinghN.SwainS.SinghA.NandiA. K. (2018). AtOZF1 positively regulates defense against bacterial pathogens and NPR1-independent salicylic acid signaling.Mol. Plant Microbe Interact.31323–333. 10.1094/MPMI-08-17-0208-R
97
SmaczniakC.LiN.BoerenS.AmericaT.van DongenW.GoerdayalS. S.et al (2012). Proteomics-based identification of low-abundance signaling and regulatory protein complexes in native plant tissues.Nat. Protoc.72144–2158. 10.1038/nprot.2012.129
98
SolisG. P.HoeggM.MunderlohC.SchrockY.Malaga-TrilloE.Rivera-MillaE.et al (2007). Reggie/flotillin proteins are organized into stable tetramers in membrane microdomains.Biochem. J.403313–322. 10.1042/bj20061686
99
SpeersA. E.WuC. C. (2007). Proteomics of integral membrane proteins-theory and application.Chem. Rev.1073687–3714. 10.1021/cr068286z
100
TanS.TanH. T.ChungM. C. M. (2008). Membrane proteins and membrane proteomics.Proteomics83924–3932. 10.1002/pmic.200800597
101
ten HaveS.BoulonS.AhmadY.LamondA. I. (2011). Mass spectrometry-based immuno-precipitation proteomics - The user’s guide.Proteomics111153–1159. 10.1002/pmic.201000548
102
UehleinN.SperlingH.HeckwolfM.KaldenhoffR. (2012). The Arabidopsis aquaporin PIP1;2 rules cellular CO2 uptake.Plant Cell Environ.351077–1083. 10.1111/j.1365-3040.2011.02473.x
103
UllrichA.SchlessingerJ. (1990). Signal transduction by receptors with tyrosine kinase-activity.Cell61203–212. 10.1016/0092-8674(90)90801-K
104
Van LeeneJ.EeckhoutD.CannootB.De WinneN.PersiauG.Van De SlijkeE.et al (2015). An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes.Nat. Protoc.10169–187. 10.1038/nprot.2014.199
105
VaretA.HauseB.HauseG.ScheelD.LeeJ. (2003). The Arabidopsis NHL3 gene encodes a plasma membrane protein and its overexpression correlates with increased resistance to Pseudomonas syringae pv. tomato DC3000.Plant Physiol.1322023–2033. 10.1104/pp.103.020438
106
VaretA.ParkerJ.TorneroP.NassN.NürnbergerT.DanglJ. L.et al (2002). NHL25 and NHL3, two NDR1/HIN-like genes in Arabidopsis thaliana with potential role(s) in plant defense.Mol. Plant Microbe Interact.15:608. 10.1094/MPMI.2002.15.6.608
107
VolontéD.GalbiatiF.LiS.NishiyamaK.OkamotoT.LisantiM. P. (1999). Flotillins/cavatellins are differentially expressed in cells and tissues and form a hetero-oligomeric complex with caveolins in vivo: characterization and epitope-mapping of a novel flotillin-1 monoclonal antibody probe.J. Biol. Chem.27412702–12709. 10.1074/jbc.274.18.12702
108
WangL.LiH.LvX. Q.ChenT.LiR. L.XueY. Q.et al (2015). Spatiotemporal dynamics of the bri1 receptor and its regulation by membrane microdomains in living Arabidopsis cells.Mol. Plant81334–1349. 10.1016/j.molp.2015.04.005
109
WeiT. Y.ZhangC. W.HouX. L.SanfaconH.WangA. M. (2013). The SNARE protein SYP71 is essential for turnip mosaic virus infection by mediating fusion of virus-induced vesicles with chloroplasts.PLoS Pathog.9:e1003378. 10.1371/journal.ppat.1003378
110
WeisC.PfeilmeierS.GlawischnigE.IsonoE.PachlF.HahneH.et al (2013). Co-immunoprecipitation-based identification of putative BAX INHIBITOR-1-interacting proteins involved in cell death regulation and plant-powdery mildew interactions.Mol. Plant Pathol.14791–802. 10.1111/mpp.12050
111
XingS.WallmerothN.BerendzenK. W.GrefenC. (2016). Techniques for the analysis of protein-protein interactions in vivo.Plant Physiol.171727–758. 10.1104/pp.16.00470
112
YamauchiS.TakemiyaA.SakamotoT.KurataT.TsutsumiT.KinoshitaT.et al (2016). The plasma membrane H+-ATPase AHA1 plays a major role in stomatal opening in response to blue light.Plant Physiol.1712731–2743. 10.1104/pp.16.01581
113
YanS. L.McLamoreE. S.DongS. S.GaoH. B.TaguchiM.WangN. N.et al (2015). The role of plasma membrane H+-ATPase in jasmonate-induced ion fluxes and stomatal closure in Arabidopsis thaliana.Plant J.83638–649. 10.1111/tpj.12915
114
YuM.LiuH. J.DongZ. Y.XiaoJ. W.SuB. D.FanL. S.et al (2017). The dynamics and endocytosis of Flot1 protein in response to flg22 in Arabidopsis.J. Plant Physiol.21573–84. 10.1016/j.jplph.2017.05.010
115
ZelaznyE.BorstJ. W.MuylaertM.BatokoH.HemmingaM. A.ChaumontF. (2007). FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization.Proc. Natl. Acad. Sci. U.S.A.10412359–12364. 10.1073/pnas.0701180104
116
ZhengM. S.TakahashiH.MiyazakiA.HamamotoH.ShahJ.YamaguchiI.et al (2004). Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signalling pathways that differ in salicylate involvement.Planta218740–750. 10.1007/s00425-003-1169-2
117
ZhouL.CheungM. Y.ZhangQ.LeiC. L.ZhangS. H.SunS. S.et al (2009). A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1).Plant Cell Environ.321804–1820. 10.1111/j.1365-3040.2009.02039.x
118
ZhouL. A.CheungM. Y.LiM. W.FuY. P.SunZ. X.SunS. M.et al (2010). Rice hypersensitive induced reaction protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death.Bmc Plant Biol.10:290. 10.1186/1471-2229-10-290
119
ZhouZ. Y.WuY. J.YangY. Q.DuM. M.ZhangX. J.GuoY.et al (2015). An Arabidopsis plasma membrane proton ATPase modulates ja signaling and is exploited by the pseudomonas syringae effector protein avrb for stomatal invasion.Plant Cell272032–2041. 10.1105/tpc.15.00466
Summary
Keywords
Arabidopsis flotillin 2, protein–protein interactions, immunopurification, mass spectrometry, split-ubiquitin yeast system, plant–pathogen interaction, water transport, intracellular trafficking
Citation
Junková P, Daněk M, Kocourková D, Brouzdová J, Kroumanová K, Zelazny E, Janda M, Hynek R, Martinec J and Valentová O (2018) Mapping of Plasma Membrane Proteins Interacting With Arabidopsis thaliana Flotillin 2. Front. Plant Sci. 9:991. doi: 10.3389/fpls.2018.00991
Received
28 February 2018
Accepted
19 June 2018
Published
12 July 2018
Volume
9 - 2018
Edited by
Ive De Smet, Flanders Institute for Biotechnology, Belgium
Reviewed by
Thomas Ott, Albert-Ludwigs-Universität Freiburg, Germany; Xia Wu, University of Washington, United States; Jinxing Lin, Beijing Forestry University, China; Ruili Li, Beijing Forestry University, China
Updates
Copyright
© 2018 Junková, Daněk, Kocourková, Brouzdová, Kroumanová, Zelazny, Janda, Hynek, Martinec and Valentová.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Petra Junková, Petra.Junkova@vscht.cz; pjova13@gmail.com
†These authors have contributed equally to this work.
This article was submitted to Plant Proteomics, a section of the journal Frontiers in Plant Science
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.