Frontiers in Plant Science | Plant Biotechnology

Original Research

Published on 16 Jan 2026

Isolation and transformation of perennial ryegrass (Lolium perenne L.) protoplasts for the in vivo assessment of guide RNAs editing efficiency

in Plant Biotechnology

Ferenz Sustek-Sánchez
Erki Eelmets
Lenne Nigul
Kairi Kärblane
Martin Laasmaa
Madara Balode-Sausina
Sanda Astra Berzina
Davis Ducis
Elza Kaktina
Kristina Jaškūne

Frontiers in Plant Science

doi 10.3389/fpls.2025.1744085

885 views

Original Research

Accepted on 14 Jan 2026

Expression of the peanut diacylglycerol acyltransferase 3 increases the neutral lipid content and improves the fatty acid composition of Chlorella vulgaris

in Plant Biotechnology

Moran Topf
Anna Andreeva
Helen Saul
Tamar Tenenvorzel
Lotem Davidi-Shwarts
Zehavit Dadon
Irina Berezin
Yael Kinel-Tahan
Yaron Yehoshua
Orit Shaul

Frontiers in Plant Science

246 views

Original Research

Published on 13 Jan 2026

Thidiazuron and trans-zeatin riboside promote development of shoots and nodes in in vitro propagation of hops (Humulus lupulus L. var. lupulus)

in Plant Biotechnology

Elise R. Staats
Maria M. Jenderek

Frontiers in Plant Science

doi 10.3389/fpls.2025.1694038

671 views

Review

Accepted on 12 Jan 2026

Advances in the use of Morphogenic Regulators and peptide regenerating factors for expedited Plant Transformation and Gene Editing

in Plant Biotechnology

Mohan Murali Achary
EASTER DAVID
Simranjit Kaur
Katamreddy Sri Cindhuri
Zou Danni
Sarah J Hearne
Anindya Bandyopadhyay

Frontiers in Plant Science

324 views

Original Research

Published on 09 Jan 2026

CRISPR/Cas9-mediated inactivation of the soybean agglutinin Le1 gene to improve grain quality

in Plant Biotechnology

João Matheus Kafer
Alessandra Koltun
Rodrigo Thibes Hoshino
Larissa Girotto
Cesar Augusto Silveira
Silvana Regina Rockenbach Marin
Elibio Leopoldo Rech
Alexandre Lima Nepomuceno
Liliane Marcia Mertz-Henning

Frontiers in Plant Science

doi 10.3389/fpls.2025.1737513

1,381 views

Perspective

Published on 09 Jan 2026

Agrochemical innovation for crop health: moving forward through dynamic disorder

in Plant Biotechnology

An-Shan Hsiao

Frontiers in Plant Science

doi 10.3389/fpls.2025.1719658

1,046 views

Editorial

Published on 05 Jan 2026

Editorial: Plant molecular farming for biopharmaceutical production and beyond

in Plant Biotechnology

Kevin Yueju Wang
Yongfeng Guo

Frontiers in Plant Science

doi 10.3389/fpls.2025.1765245

1,023 views

Brief Research Report

Published on 02 Jan 2026

Production of functional human galectin-1 in transplastomic tobacco and simplified recovery via batch-mode purification

in Plant Biotechnology

Catalina Francisca Vater
Juan Manuel Pérez Sáez
Juan Carlos Stupirski
Mora Massaro
Federico Gabriel Mirkin
Fernando Félix Bravo-Almonacid
Gabriel Adrián Rabinovich
Mauro Miguel Morgenfeld

Frontiers in Plant Science

doi 10.3389/fpls.2025.1721928

1,382 views

Original Research

Published on 02 Jan 2026

Clinical-grade plant-made nanomaterials: from process design to the construction of a manufacturing facility

in Plant Biotechnology

Denise Pivotto
Anthony Rosa
Aya Maged Elsheikh
Elisa Gecchele
Roberta Zampieri
Alessia Raneri
Valentina Garonzi
Linda Avesani

Frontiers in Plant Science

doi 10.3389/fpls.2025.1724810

1,331 views
1 citation

Review

Accepted on 30 Dec 2025

CRISPR/Cas Technique: Revolutionizing Solanaceae Crop Improvement or CRISPR/Cas: A Precision tool for solanaceae crop Improvement or Genome Editing in Solanaceae: Harnessing CRISPR-Cas technology for Precision Crop Improvement

in Plant Biotechnology

Vandana Thakur
VIshal Johar
Vinita BISHT
Shalu Vyas
Akanksha Singh
Pratyksh Pandey
Bhalendra Singh Rajput
L. S. Kandari
Eshetu Yilma

Frontiers in Plant Science

461 views

Original Research

Accepted on 24 Dec 2025

Effects of acute and chronic gamma irradiations on regeneration of tissue types from selected Ugandan cassava genotypes

in Plant Biotechnology

Hellen Apio
Emmanuel Ogwok
Noralzina B. Noordin
Peter Waswa
Titus Alicai
John Odipio
Settumba B. Mukasa
Isaac Kofi Bimpong

Frontiers in Plant Science

119 views

Perspective

Published on 16 Dec 2025

Leveraging tobacco for a low-carbon biorefinery

in Plant Biotechnology

Quanyu Yin
Zhengkang Zhu
Yujia Chen
Mengquan Yang

Frontiers in Plant Science

doi 10.3389/fpls.2025.1662412

1,366 views

Original Research

Published on 12 Dec 2025

Multiplex CRISPR/Cas9-mediated editing of seven glycosyltransferase homologs in Nicotiana benthamiana to produce stable, Cas9-free, glycoengineered plants

in Plant Biotechnology

Chetan Kaur
Hayoung Song
Myungjin Lee
Seo-Young Kim
Dong-Hoon Seo
Hyangju Kang
Eun-Ju Sohn
Yidong Ran
Okjae Koo
Geung-Joo Lee

Frontiers in Plant Science

doi 10.3389/fpls.2025.1701668

1,787 views
1 citation

Original Research

Published on 09 Dec 2025

Humic field biostimulation as a sustainable agricultural practice to increase yield of main grains: evidence from on-farm trials

in Plant Biotechnology

Juan Izquierdo
Osvin Arriagada
Gustavo García-Pintos
Rodomiro Ortiz
Martín García-Pintos
Marcelo García-Pintos

Frontiers in Plant Science

doi 10.3389/fpls.2025.1709876

1,231 views

Original Research

Published on 04 Dec 2025

Genetic dissection of nutrient element enrichment in Saccharum officinarum L. for deciphering the food health

in Plant Biotechnology

Lina Fan
Zaid Chachar
Ming Chen
Yucong Li
Jiarui Chen
Huiyi He
Jiakun Wen
Saira Gul
Ruiqiang Lai
Yongwen Qi

Frontiers in Plant Science

doi 10.3389/fpls.2025.1649792

1,066 views

Review

Published on 04 Dec 2025

Advances and challenges in plant N-glycoengineering: when fucosylation matters

in Plant Biotechnology

Kavya Gauba
Vinny Kunnummel
Alexandra Castilho

Frontiers in Plant Science

doi 10.3389/fpls.2025.1734060

1,499 views

Original Research

Published on 02 Dec 2025

Comprehensive evaluation of transgenic rice lines expressing CSFV E2 protein in genetic stability, environmental safety and field adaptation

in Plant Biotechnology

Hongyan Chu
Lei Zhang
Zhen Hao
Yupeng Hua
Wenjing Hu
Ruijing Liu
Yanyue Lou
Wenming Gao
Shuangli Bian
Fei Li

Frontiers in Plant Science

doi 10.3389/fpls.2025.1650765

1,016 views

Review

Published on 01 Dec 2025

Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases

in Plant Biotechnology

Marcos Fernando Basso
Maisa de Siqueira Pinto
Juliana Vieira Almeida Nonato
Ricardo Augusto Dante
Juliana Erika de Carvalho Teixeira Yassitepe

Frontiers in Plant Science

doi 10.3389/fpls.2025.1712585

2,040 views

Original Research

Published on 28 Nov 2025

Nano-elicitation strategy to improve specialized metabolite pathways in plant cell suspension culture

in Plant Biotechnology

Kamran Ashraf
Qamar uz Zaman
Weihua Tang
Pei Jiang
Xun Wan
Khitev Yuri
Ali Mohsin
Meijin Guo

Frontiers in Plant Science

doi 10.3389/fpls.2025.1679901

1,547 views
1 citation

Original Research

Published on 27 Nov 2025

Single-cell RNA sequencing reveals cellular diversity and gene expression dynamics in maize root development

in Plant Biotechnology

Jianwen Bian
Zelong Zhuang
Rui Tang
Wanling Ta
Zhenping Ren
Yunling Peng

Frontiers in Plant Science

doi 10.3389/fpls.2025.1666531

1,941 views
1 citation

Original Research

Published on 26 Nov 2025

An innovative infection method for the accumulation of viral nanoparticles in Nicotiana benthamiana

in Plant Biotechnology

Kristina Ljumović
Anthony Rosa
Alessia Raneri
Matteo Ballottari
Linda Avesani
Nico Betterle

Frontiers in Plant Science

doi 10.3389/fpls.2025.1727190

1,460 views

Editorial

Published on 20 Nov 2025

Editorial: Plant-based solutions for sustainable agriculture and environmental remediation

in Plant Biotechnology

Carla S. Santos
Mehrdad Zarafshar
Marta Nunes da Silva

Frontiers in Plant Science

doi 10.3389/fpls.2025.1729054

1,012 views

Original Research

Published on 04 Nov 2025

Oryzalin-induced polyploidy in Borago officinalis reveals cell-wall remodelling via immunofluorescence microscopy

in Plant Biotechnology

Josef Baltazar Šenkyřík
Anna Milewska-Hendel
Daniel Král
Vladan Ondřej

Frontiers in Plant Science

doi 10.3389/fpls.2025.1676435

1,914 views

Original Research

Published on 30 Oct 2025

A WRKY transcription factor, SlWRKY75, positively regulates tomato (Solanum lycopersicum L.) resistance to Ralstonia solanacearum

in Plant Biotechnology

Na Chen
Lingzeng Lv
Lian Duan
Jiajun Wu
Qin Shao
Xiaopeng Li
Qineng Lu

Frontiers in Plant Science

doi 10.3389/fpls.2025.1704937

2,339 views
1 citation